Advanced Microscopy

This course is organized by the Leeuwenhoek Centre for Advanced Microscopy (LCAM) and provides an in-depth view of specific advanced imaging techniques used at imaging centers of AMC, NKI, VUmc and FNWI.

This course runs once a year for three consecutive days.
The course focuses fully on intensive hands-on practical sessions and interactive discussions with experts in the various advanced fluorescence microscope techniques. On the final day participants present their own research topic and discuss which techniques should be implemented in their project.

The main part of the course will take place at the AMC (core facility Cellular Imaging) with also lectures and demonstrations from three other institutes in Amsterdam: the NKI (Dutch Cancer Institute), the FNWI (UvA Science Park), and the VUmc. The participants will focus in depth on specific advanced imaging techniques, including:

1. Measuring intra- and inter-molecular protein interactions using FRET and FLIM

2. Confocal microscopy methods (CLSM)

3. Super resolution microscopy

4. Confocal quality control

5. Correlative microscopy (bridging fluorescence microscopy and electron microscopy)

6. Advanced electron microscopy

7. Usage of fluorophores such as FlAsH/ReAsH.

Scheduled dates:

Target audience:
PhD candidates and postdocs. An introduction course like the Basic Microscopy Course is a prerequisite for the Advanced Microscopy Course.

Study load:
44 hours, which is comparable to 1.6 ECTS points.

Course organizers:
Eric Reits (AMC) and Mark Hink (FNWI).

Application and more information:
For information on OOA courses, and to register, please visit the OOA website:

Eric Reits / Department of Celbiology & Histology / / tel. +31 (0)20 566 6259


AMC Graduate School
Tel: +31 (0)20 - 5663108