Mass Spectrometry, Proteomics and Protein Research

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This course trains the participants, both practically and theoretically, in advanced techniques for the isolation and analysis of proteins.

This intensive five-day course runs once or twice a year for a full week (Monday to Friday, 9.00 - 17.00 hrs).

The ratio of practical to theory work is about 60:40. Participants receive a course manual describing the experimental protocols, as well as the theoretical background to the experimental steps. The theory component includes short lectures and self-training sessions using computer simulation programs. Seminars on related subjects are also provided.

This course is organized by the AMC Department of Medical Biochemistry, with members of its scientific staff providing the lectures and practical assistance to participants. Other departments, such as Bio-Informatics, are collaborating in specific subject areas.

Scheduled dates:
October 30 thru November 3, 2017

April 9 thru 13, 2018

Important improvements have recently been made in their mass spectrometric analysis. Participants are provided with background information, with a feel for the pitfalls and possibilities of state-of-the-art proteomic research, and with ‘hands­-on’ experience in a number of important experimental procedures used in modern protein research.

The subjects covered are:
1. Isolation of proteins with column chromatography
2. Gel electrophoresis and protein staining
3. Tryptic in gel digestion of proteins
4. MALDI/ SELDI­-TOF technology
5. Protein sequencing: LC-ESI-ms/ms technology
6. Biomarker discovery.

Target audience:
AMC PhD candidates and other participants who have experience with life science studies at the molecular level, as well as some basic knowledge of physics, chemistry and molecular biology. For those in any doubt as to whether they possess sufficient knowledge of these topics, the intensive one-day crash course is available.

The course is given in English.

To qualify for the certificate, a participant must attend all lectures and workshops, and complete all assignments satisfactorily. Attendance is registered.

Study load:
60 hours, which is comparable to 2.1 ECTS points.

Number of participants:
Maximum 8 per course.

No charge for registered AMC PhD candidates and employees of the AMC or AMC Medical Research BV. Other participants are charged a fee of 750 euro (non-­profit sector) or 1,500 euro (commercial sector).

Please note that this course requires considerable investment of time and money, including the purchase of expensive chemicals. For that reason, the expenses incurred will be charged to the participant in the event of cancellation within two weeks prior to the start of the course.

Course coordinator:
Dr. D. Speijer / Dep. of Medical Biochemistry / / tel. +31 (0)20 566 5134

More information:
From the course coordinator or A. van de Woestijne / Dep. of Medical Biochemistry / / tel. +31 (0)20 566 5159


AMC Graduate School
Tel: +31 (0)20 - 5663108