van Leeuwenhoek Centre for Advanced Microscopy LCAM-AMC

The core facility Cellular Imaging is embedded in the Department of Cell Biology & Histology, where it is linked with various research themes and PI groups, and the teaching of students and researchers via a variety of imaging courses.

The main techniques are:

Click on the titles below to few the details of these techniques.

Flow cytometry: cell sorting and analysis

The cell sorting and analyzing facility is located at the department of Cell Biology at L3-139, and is the central core facility for all AMC users. Two experienced operators can help with carrying out sorting experiments. The daily management is in hands of Berend Hooibrink.

  • Analysis instruments:
    • LSR-Fortessa (5 laser excitation, 18 color fluorescence detection)
    • FacscantoII (2 laser excitation, 8 color fluorescence, automated multiplate sample loader for high throughput).
  • Cellsorters:
    • Facsaria (3 laser excitation, 9 color, 4 population sorting and clone provision)
    • Facsaria-sorp (4 laser excitation, 13 color, 4 population sorting and clone provision)
    • Influx (4 laser excitation, 14 color fluorescence detection, 6 population sorting and clone provision)

What should you do if you want to make use of the facility?
You can make an appointment to use the cell sorters via the website http://www.lcam-amc.nl/
For advice you can contact the operators Berend Hooibrink, b.hooibrink@amc.nl and Toni van Capel, t.m.vancapel@amc.uva.nl.

Advanced fluorescence microscopy

The advanced fluorescence microscopy is mainly located within the department of Cell Biology at M3, including the confocal, superresolution and upright microscopes. The inverted fluorescence microscopes for automated live cell imaging and micro-injection are located at  L3-140.

The advanced fluorescence microscopy is embedded in the Van Leeuwenhoek Centre for Advanced Microscopy (LCAM), a collaboration between the AMC, the Faculty of Science (FNWI) of the University of Amsterdam and the Netherlands Cancer Institute (NKI). The LCAM-AMC offers visualization and microscopic analysis of cell organelles, cells and tissues. It houses a wide range of instrumentation for electron-, fluorescence-, confocal- and living cell microscopy.

Microscopes:

  • Confocal microscope, Leica SP8 (white light laser, small incubator, resonance scanner)
  • Confocal microscope, Leica SP8 (white light laser, incubator, FRET-FLIM SMD)
  • Leica SR GSD Super-resolution microscope (includes TIRF)
  • 2 upright fluorescence microscopes, Leica DMRA & Leica DMRXA equipped with a high sensitive CCD camera
  • Upright histology microscope, Leica
  • Inverted phase-contrast/fluorescence microscope for long time monitoring of living cells, Leica IRBE
  • Inverted fluorescence microscope with micro-injector, Leica IRBE

What should you do if you want to make use of the facility?
You can make an appointment to use the microscopes via the website http://www.lcam-amc.nl/

For advice you can contact the operators:

  • Confocal: Ron Hoebe, r.a.hoebe@amc.nl or Daisy Picavet, d.i.picavet@amc.uva.nl
  • Wide-field (live-cell) fluorescence microscopy: Jan Stap, j.stap@amc.nl
  • How to translate your research project to microscopy: Eric Reits, e.a.reits@amc.uva.nl

Other techniques offered by allied institutes include fluorescence correlation spectroscopy (FCS), spinning disk confocal, 2-photon and confocal high-content screening microscopy.

Electron microscopy

The electron microscopy of the AMC is located within the department of Cell Biology at M3, and is used both for diagnosis (pathology) and research projects.

Microscopes and techniques available are:

  • transmission electron microscopes: Fei Technai-12 & Philips CM-10
  • scanning electron microscope, Philips
  • freeze fracturing and EM tomography
  • immuno EM

What should you do if you want to make use of the facility?
You can make an appointment via the website http://www.lcam-amc.nl/
Contact for electron microscopy: Nicole van der Wel, n.n.vanderwel@amc.uva.nl
Operators include Henk van Veen, h.a.vanveen@amc.uva.nl and Marius van den Bergh Weerman,m.a.vdberghweerman@amc.uva.nl

The facility includes various microscopic and cytometry techniques and instrumentation, and the software for analysis, restoration and reconstruction related to electron tomography and confocal microscopy as well as for the analysis of the movement of/in living cells. The facility also organizes courses (both basic and advanced) for PhD students and other scientists, and is involved in various courses for bachelor and master students.

The facility is open for all researchers from within the AMC, as well as from outside the AMC (especially within the alliance with the VUmc, but also for the UvA, NIN, ACTA, NKI and Sanquin), who can also use the central workstation unit to work on the acquired data. People can contact operators and schedule their experiments via the website and intranet. Central contactperson is Eric Reits e.a.reits@amc.uva.nl.

Contact

Confocal microscopy
Ron Hoebe
Tel: +31(0)20-5664743
Daisy Picavet
Tel: +31(0)20-5660080

Electron microscopy
Nicole van der Wel
Tel: +31(0)20-5664757
Henk van Veen
Tel: +31(0)20-5664703

Fluorescence microscopy
Jan Stap
Tel: +31(0)20-5664771
Ron Hoebe
Tel: +31(0)20-5664743

Flow cytometry and cell sorting
Berend Hooibrink
Toni van Capel
Tel: +31(0)20-5666804

Central contactperson
Eric Reits
Tel: +31(0)20-5666259

For more information visit us at AMC, location M3.