• Principal Investigator
  • Male

Prof. dr. V.M. Christoffels

Position: Professor
Main activities: Research, Teaching
Specialisation: developmental biology, molecular biology, heart development
foto V.M. Christoffels is one of the AMC Principal Investigators
Focus of research:
  • Developmental biology
  • Heart development
  • Transcriptional regulation
  • Epigenetics
  • Congenital heart defects
  • Conduction system
  • Sinoatrial node
  • Arrhythmias
  • Transgenic mice
  • Lineage analysis
AMC themes: Cardiovascular Diseases, Reproduction and development
Departments: Medical Biology
Anas AA, Claushuis TAM, Mohan RA, Christoffels VM, Aidinis V, Florquin S, van't Veer C, Hou B, de Vos AF, van der Poll T, Epithelial Myeloid-Differentiation Factor 88 Is Dispensable during Klebsiella Pneumonia. AM J RESP CELL MOL 2017;56 (5):648-656 [PubMed]
Jensen B, van der Wal AC, Moorman AFM, Christoffels VM, Excessive trabeculations in noncompaction do not have the embryonic identity. INT J CARDIOL 2017;227:325-330 [PubMed]
Jensen B, Vesterskov S, Boukens BJ, Nielsen JM, Moorman AFM, Christoffels VM, Wang T, Morpho-functional characterization of the systemic venous pole of the reptile heart. SCI REP-UK 2017;7 (1):6644 [PubMed]
van den Boogaard M. Transcriptional control of ion channel genes in arrhythmogenesis. S.l.: s.n.; 2017. 247p. ISBN 978-90-824887-2-2 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Christoffels VM; copromotor(s): Barnett P)
Boukens BJ, Coronel R, Christoffels VM, Embryonic development of the right ventricular outflow tract and arrhythmias. HEART RHYTHM 2016;13 (2):616-622 [PubMed]
Burger NB. Developmental origins of increased nuchal translucency. S.l.: s.n.; 2016. 193p. ISBN 9789462993846 Proefschrift Vrije Universiteit Amsterdam, Faculteit Geneeskunde. (Promotor(s): de Groot CJM, Christoffels VM; copromotor(s): Bekker MN, Haak MC)
Burger NB, Haak MC, Kok E, de Groot CJM, Shou W, Scambler PJ, Lee Y, Cho E, Christoffels VM, Bekker MN, Cardiac defects, nuchal edema and abnormal lymphatic development are not associated with morphological changes in the ductus venosus. EARLY HUM DEV 2016;101:39-48 [PubMed]
Burger NB, Matias A, Kok E, de Groot CJM, Christoffels VM, Bekker MN, Haak MC, Absence of an anatomical origin for altered ductus venosus flow velocity waveforms in first-trimester human fetuses with increased nuchal translucency. PRENATAL DIAG 2016;36 (6):537-544 [PubMed]
de Bakker BS, de Jong KH, Hagoort J, de Bree K, Besselink CT, de Kanter FEC, Veldhuis T, Bais B, Schildmeijer R, Ruijter JM, Oostra RJ, Christoffels VM, Moorman AFM, An interactive three-dimensional digital atlas and quantitative database of human development. SCIENCE 2016;354 (6315):1019 aag0053-+ [PubMed]
Greulich F, Rudat C, Farin HF, Christoffels VM, Kispert A, Lack of Genetic Interaction between Tbx18 and Tbx2/Tbx20 in Mouse Epicardial Development. PLOS ONE 2016;11 (6):e0156787 [PubMed]
Lüdtke TH, Rudat C, Wojahn I, Weiss AC, Kleppa MJ, Kurz J, Farin HF, Moon A, Christoffels VM, Kispert A, Tbx2 and Tbx3 Act Downstream of Shh to Maintain Canonical Wnt Signaling during Branching Morphogenesis of the Murine Lung. DEV CELL 2016;39 (2):239-253 [PubMed]
Nadadur RD, Broman MT, Boukens B, Mazurek SR, Yang X, van den Boogaard M, Bekeny J, Gadek M, Ward T, Zhang M, Qiao Y, Martin JF, Seidman CE, Seidman J, Christoffels V, Efimov IR, McNally EM, Weber CR, Moskowitz IP, Pitx2 modulates a Tbx5-dependent gene regulatory network to maintain atrial rhythm. SCI TRANSL MED 2016;8 (354):354ra115 [PubMed]
Postma AV, Bezzina CR, Christoffels VM, Genetics of congenital heart disease: the contribution of the noncoding regulatory genome. J HUM GENET 2016;61 (1):13-19 [PubMed]
Sergeeva IA, Hooijkaas IB, Ruijter JM, van der Made I, de Groot NE, van de Werken HJG, Creemers EE, Christoffels VM, Identification of a regulatory domain controlling the Nppa-Nppb gene cluster during heart development and stress. DEVELOPMENT 2016;143 (12):2135-2146 [PubMed]
van der Harst P, van Setten J, Verweij N, Vogler G, Franke L, Maurano MT, Wang X, Mateo Leach I, Eijgelsheim M, Sotoodehnia N, Hayward C, Sorice R, Meirelles O, Lyytikäinen LP, Polašek O, Tanaka T, Arking DE, Ulivi S, Trompet S, Müller-Nurasyid M, Smith AV, Dörr M, Kerr KF, Magnani JW, del Greco M F, Zhang W, Nolte IM, Silva CT, Padmanabhan S, Tragante V, Esko T, Abecasis GR, Adriaens ME, Andersen K, Barnett P, Bis JC, Bodmer R, Buckley BM, Campbell H, Cannon MV, Chakravarti A, Chen LY, Delitala A, Devereux RB, Doevendans PA, Dominiczak AF, Ferrucci L, Ford I, Gieger C, Harris TB, Haugen E, Heinig M, Hernandez DG, Hillege HL, Hirschhorn JN, Hofman A, Hubner N, Hwang SJ, Iorio A, Kähönen M, Kellis M, Kolcic I, Kooner IK, Kooner JS, Kors JA, Lakatta EG, Lage K, Launer LJ, Levy D, Lundby A, Macfarlane PW, May D, Meitinger T, Metspalu A, Nappo S, Naitza S, Neph S, Nord AS, Nutile T, Okin PM, Olsen JV, Oostra BA, Penninger JM, Pennacchio LA, Pers TH, Perz S, Peters A, Pinto YM, Pfeufer A, Pilia MG, Pramstaller PP, Prins BP, Raitakari OT, Raychaudhuri S, Rice KM, Rossin EJ, Rotter JI, Schafer S, Schlessinger D, Schmidt CO, Sehmi J, Silljé HHW, Sinagra G, Sinner MF, Slowikowski K, Soliman EZ, Spector TD, Spiering W, Stamatoyannopoulos JA, Stolk RP, Strauch K, Tan ST, Tarasov KV, Trinh B, Uitterlinden AG, van den Boogaard M, van Duijn CM, van Gilst WH, Viikari JS, Visscher PM, Vitart V, Völker U, Waldenberger M, Weichenberger CX, Westra HJ, Wijmenga C, Wolffenbuttel BH, Yang J, Bezzina CR, Munroe PB, Snieder H, Wright AF, Rudan I, Boyer LA, Asselbergs FW, van Veldhuisen DJ, Stricker BH, Psaty BM, Ciullo M, Sanna S, Lehtimäki T, Wilson JF, Bandinelli S, Alonso A, Gasparini P, Jukema JW, Kääb S, Gudnason V, Felix SB, Heckbert SR, de Boer RA, Newton-Cheh C, Hicks AA, Chambers JC, Jamshidi Y, Visel A, Christoffels VM, Isaacs A, Samani NJ, de Bakker PIW, 52 Genetic Loci Influencing Myocardial Mass. J AM COLL CARDIOL 2016;68 (13):1435-1448 [PubMed]
van Duijvenboden K, de Boer BA, Capon N, Ruijter JM, Christoffels VM, EMERGE: a flexible modelling framework to predict genomic regulatory elements from genomic signatures. NUCLEIC ACIDS RES 2016;44 (5):e42 [PubMed]
van Weerd JH, Christoffels VM. Regulation of Vertebrate Conduction System Development in: Nakanishi T, Markwald RR, Baldwin HS, Keller BB, Srivastava D, Yamagishi H, editors. Etiology and Morphogenesis of Congenital Heart Disease: From Gene Function and Cellular Interaction to Morphology. S.l.: Springer Open; 2016., p. 269-280
van Weerd JH, Christoffels VM, The formation and function of the cardiac conduction system. DEVELOPMENT 2016;143 (2):197-210 [PubMed]
Boink GJJ, Christoffels VM, Robinson RB, Tan HL, The past, present, and future of pacemaker therapies. TRENDS CARDIOVAS MED 2015;25 (8):661-673 [PubMed]
Burger NB, Bekker MN, de Groot CJM, Christoffels VM, Haak MC, Why increased nuchal translucency is associated with congenital heart disease: a systematic review on genetic mechanisms. PRENATAL DIAG 2015;35 (6):517-528 [PubMed]
Burger NB, Bekker MN, Kok E, de Groot CJM, Martin JF, Shou W, Scambler PJ, Lee Y, Christoffels VM, Haak MC, Increased nuchal translucency origins from abnormal lymphatic development and is independent of the presence of a cardiac defect. PRENATAL DIAG 2015;35 (13):1278-1286 [PubMed]
Gillers BS, Chiplunkar A, Aly H, Valenta T, Basler K, Christoffels VM, Efimov IR, Boukens BJ, Rentschler S, Canonical wnt signaling regulates atrioventricular junction programming and electrophysiological properties. CIRC RES 2015;116 (3):398-406 [PubMed]
Sergeeva IA. Functional organization of the natriuretic peptide gene cluster in development and disease. S.l.: s.n.; 2015. 141p. ISBN 978-94-6182-594-0 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Christoffels VM; copromotor(s): )
Stefanovic S, Christoffels VM, GATA-dependent transcriptional and epigenetic control of cardiac lineage specification and differentiation. CELL MOL LIFE SCI 2015;72 (20):3871-3881 [PubMed]
Burger NB, Stuurman KE, Kok E, Konijn T, Schooneman D, Niederreither K, Coles M, Agace WW, Christoffels VM, Mebius RE, van de Pavert SA, Bekker MN, Involvement of neurons and retinoic acid in lymphatic development: new insights in increased nuchal translucency. PRENATAL DIAG 2014;34 (13):1312-1319 [PubMed]
Burggren WW, Christoffels VM, Crossley DA 2nd, Enok S, Farrell AP, Hedrick MS, Hicks JW, Jensen B, Moorman AFM, Mueller CA, Skovgaard N, Taylor EW, Wang T, Comparative cardiovascular physiology: future trends, opportunities and challenges. ACTA PHYSIOL 2014;210 (2):257-276 [PubMed]
Davies L, Jin J, Shen W, Tsui H, Shi Y, Wang Y, Zhang Y, Hao G, Wu J, Chen S, Fraser JA, Dong N, Christoffels V, Ravens U, Huang CLH, Zhang H, Cartwright EJ, Wang X, Lei M, Mkk4 is a negative regulator of the transforming growth factor beta 1 signaling associated with atrial remodeling and arrhythmogenesis with age. J AM HEART ASSOC 2014;3 (2):e000340 [PubMed]
de Boer BA, Le Garrec JF, Christoffels VM, Meilhac SM, Ruijter JM, Integrating multi-scale knowledge on cardiac development into a computational model of ventricular trabeculation. WIRES SYST BIOL MED 2014;6 (6):389-397 [PubMed]
de Boer BA, van Duijvenboden K, van den Boogaard M, Christoffels VM, Barnett P, Ruijter JM, OccuPeak: ChIP-Seq peak calling based on internal background modelling. PLOS ONE 2014;9 (6):e99844 [PubMed]
Franco D, Christoffels VM, Campione M, Homeobox transcription factor Pitx2: The rise of an asymmetry gene in cardiogenesis and arrhythmogenesis. TRENDS CARDIOVAS MED 2014;24 (1):23-31 [PubMed]
Jensen B, Boukens BJD, Wang T, Moorman AFM, Christoffels VM, Evolution of the Sinus Venosus from Fish to Human. J CARDIOVASC DEV DIS 2014;1:14-28
Kunasegaran K, Ho V, Chang THT, de Silva D, Bakker ML, Christoffels VM, Pietersen AM, Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium. PLOS ONE 2014;9 (10):e110191 [PubMed]
Mohan RA, van Engelen K, Stefanovic S, Barnett P, Ilgun A, Baars MJH, Bouma BJ, Mulder BJM, Christoffels VM, Postma AV, A mutation in the Kozak sequence of GATA4 hampers translation in a family with atrial septal defects. AM J MED GENET A 2014;164AA (11):2732-2738 [PubMed]
Osterwalder M, Speziale D, Shoukry M, Mohan R , Ivanek R, Kohler M, Beisel C, Wen X, Scales SJ, Christoffels VM, Visel A, Lopez-Rios J, Zeller R, HAND2 targets define a network of transcriptional regulators that compartmentalize the early limb bud mesenchyme. DEV CELL 2014;31 (3):345-357 [PubMed]
Rana M. S. Molecular and genetic basis of congenital conotruncal heart defects. s.l.: s.n.; 2014. 178p. ISBN 9789491014291 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Christoffels VM, Moorman AFM; copromotor(s): )
Rana MS, Sizarov A, Christoffels VM, Moorman AFM, Development of the human aortic arch system captured in an interactive three-dimensional reference model. AM J MED GENET A 2014;164A (6):1372-1383 [PubMed]
Rana MS, Théveniau-Ruissy M, de Bono C, Mesbah K, Francou A, Rammah M, Domínguez JN, Roux M, Laforest B, Anderson RH, Mohun T, Zaffran S, Christoffels VM, Kelly RG, Tbx1 coordinates addition of posterior second heart field progenitor cells to the arterial and venous poles of the heart. CIRC RES 2014;115 (9):790-799 [PubMed]
Sergeeva IA, Hooijkaas IB, van der Made I, Jong WMC, Creemers EE, Christoffels VM, A transgenic mouse model for the simultaneous monitoring of ANF and BNP gene activity during heart development and disease. CARDIOVASC RES 2014;101 (1):78-86 [PubMed]
Stefanovic S, Barnett P, van Duijvenboden K, Weber D, Gessler M, Christoffels VM, GATA-dependent regulatory switches establish atrioventricular canal specificity during heart development. NAT COMMUN 2014;5:3680 [PubMed]
van den Boogaard M, Barnett P, Christoffels VM, From GWAS to function: genetic variation in sodium channel gene enhancer influences electrical patterning. TRENDS CARDIOVAS MED 2014;24 (3):99-104 [PubMed]
van den Boogaard M, Smemo S, Burnicka-Turek O, Arnolds DE, van de Werken HJG, Klous P, McKean D, Muehlschlegel JD, Moosmann J, Toka O, Yang XH, Koopmann TT, Adriaens ME, Bezzina CR, de Laat W, Seidman C, Seidman JG, Christoffels VM, Nobrega MA, Barnett P, Moskowitz IP, A common genetic variant within SCN10A modulates cardiac SCN5A expression. J CLIN INVEST 2014;124 (4):1844-1852 [PubMed]
van Duijvenboden K, Ruijter JM, Christoffels VM, Gene regulatory elements of the cardiac conduction system. BRIEF FUNCT GENOMICS 2014;13 (1):28-38 [PubMed]
van Weerd JH, Badi I, van den Boogaard M, Stefanovic S, van de Werken HJG, Gomez-Velazquez M, Badia-Careaga C, Manzanares M, de Laat W, Barnett P, Christoffels VM, A large permissive regulatory domain exclusively controls Tbx3 expression in the cardiac conduction system. CIRC RES 2014;115 (4):432-441 [PubMed]
Verweij N, Mateo Leach I, van den Boogaard M, van Veldhuisen DJ, Christoffels VM, Hillege HL, van Gilst WH, Barnett P, de Boer RA, van der Harst P, Genetic determinants of P wave duration and PR segment. CIRC-CARDIOVASC GENE 2014;7 (4):475-481 [PubMed]
Bezzina CR, Barc J, Mizusawa Y, Remme CA, Gourraud JB, Simonet F, Verkerk AO, Schwartz PJ, Crotti L, Dagradi F, Guicheney P, Fressart V, Leenhardt A, Antzelevitch C, Bartkowiak S, Borggrefe M, Schimpf R, Schulze-Bahr E, Zumhagen S, Behr ER, Bastiaenen R, Tfelt-Hansen J, Olesen MS, Kääb S, Beckmann BM, Weeke P, Watanabe H, Endo N, Minamino T, Horie M, Ohno S, Hasegawa K, Makita N, Nogami A, Shimizu W, Aiba T, Froguel P, Balkau B, Lantieri O, Torchio M, Wiese C, Weber D, Wolswinkel R, Coronel R, Boukens BJ, Bézieau S, Charpentier E, Chatel S, Despres A, Gros F, Kyndt F, Lecointe S, Lindenbaum P, Portero V, Violleau J, Gessler M, Tan HL, Roden DM, Christoffels VM, Le Marec H, Wilde AA, Probst V, Schott JJ, Dina C, Redon R, Common variants at SCN5A-SCN10A and HEY2 are associated with Brugada syndrome, a rare disease with high risk of sudden cardiac death. NAT GENET 2013;45 (9):1044-1049 [PubMed]
Bezzina CR, Barc J, Mizusawa Y, Remme CA, Gourraud JB, Simonet F, Verkerk AO, Schwartz PJ, Crotti L, Dagradi F, Guicheney P, Fressart V, Leenhardt A, Antzelevitch C, Bartkowiak S, Schulze-Bahr E, Zumhagen S, Behr ER, Bastiaenen R, Tfelt-Hansen J, Olesen MS, Kääb S, Beckmann BM, Weeke P, Watanabe H, Endo N, Minamino T, Horie M, Ohno S, Hasegawa K, Makita N, Nogami A, Shimizu W, Aiba T, Froguel P, Balkau B, Lantieri O, Torchio M, Wiese C, Weber D, Wolswinkel R, Coronel R, Boukens BJ, Bézieau S, Charpentier E, Chatel S, Despres A, Gros F, Kyndt F, Lecointe S, Lindenbaum P, Portero V, Violleau J, Gessler M, Tan HL, Roden DM, Christoffels VM, Le Marec H, Wilde AA, Probst V, Schott JJ, Dina C, Redon R, Common variants at SCN5A-SCN10A and HEY2 are associated with Brugada syndrome, a rare disease with high risk of sudden cardiac death. NAT GENET 2013;45 (11):1409 [PubMed]
Boogerd CJJ, Christoffels VM, Barnett P, Nuclear recruitment assay as a tool to validate transcription factor interactions in Mammalian cells. METHODS MOL BIOL 2013;977:243-248 [PubMed]
Boukens BJ, Hoogendijk MG, Verkerk AO, Linnenbank A, van Dam P, Remme CA, Fiolet JW, Opthof T, Christoffels VM, Coronel R, Early repolarization in mice causes overestimation of ventricular activation time by the QRS duration. CARDIOVASC RES 2013;97 (1):182-191 [PubMed]
Boukens BJ, Sylva M, de Gier-de Vries C, Remme CA, Bezzina CR, Christoffels VM, Coronel R, Reduced sodium channel function unmasks residual embryonic slow conduction in the adult right ventricular outflow tract. CIRC RES 2013;113 (2):137-141 [PubMed]
Burger NB, Haak MC, de Bakker BS, Al Shaibani Z, de Groot CJM, Christoffels VM, Bekker MN, Systematic analysis of the development of the ductus venosus in wild type mouse and human embryos. EARLY HUM DEV 2013;89 (12):1067-1073 [PubMed]
Christoffels VM, Pu WT, Developing insights into cardiac regeneration. DEVELOPMENT 2013;140 (19):3933-3937 [PubMed]
de Laat MWM, Pieper PG, Oudijk MA, Mulder BJM, Christoffels VM, Afink GB, Postma AV, Ris-Stalpers C, The clinical and molecular relations between idiopathic preterm labor and maternal congenital heart defects. REPROD SCI 2013;20 (2):190-201 [PubMed]
Farin HF, Lüdtke THW, Schmidt MK, Placzko S, Schuster-Gossler K, Petry M, Christoffels VM, Kispert A, Tbx2 terminates shh/fgf signaling in the developing mouse limb bud by direct repression of gremlin1. PLOS GENET 2013;9 (4):e1003467 [PubMed]
Francou A, Saint-Michel E, Mesbah K, Théveniau-Ruissy M, Rana MS, Christoffels VM, Kelly RG, Second heart field cardiac progenitor cells in the early mouse embryo. BBA-MOL CELL RES 2013;1833 (4):795-798 [PubMed]
Jensen B, Wang T, Christoffels VM, Moorman AFM, Evolution and development of the building plan of the vertebrate heart. BBA-MOL CELL RES 2013;1833 (4):783-794 [PubMed]
Lüdtke THW, Farin HF, Rudat C, Schuster-Gossler K, Petry M, Barnett P, Christoffels VM, Kispert A, Tbx2 controls lung growth by direct repression of the cell cycle inhibitor genes Cdkn1a and Cdkn1b. PLOS GENET 2013;9 (1):e1003189 [PubMed]
Mommersteeg MTM, Andrews WD, Ypsilanti AR, Zelina P, Yeh ML, Norden J, Kispert A, Chédotal A, Christoffels VM, Parnavelas JG, Slit-roundabout signaling regulates the development of the cardiac systemic venous return and pericardium. CIRC RES 2013;112 (3):465-475 [PubMed]
Rana MS, Christoffels VM, Moorman AFM, A molecular and genetic outline of cardiac morphogenesis. ACTA PHYSIOL 2013;207 (4):588-615 [PubMed]
Sergeeva IA, Christoffels VM, Regulation of expression of atrial and brain natriuretic peptide, biomarkers for heart development and disease. BBA-MOL BASIS DIS 2013;1832 (12):2403-2413 [PubMed]
Spruijt NE, Rana MS, Christoffels VM, Mink van der Molen AB, Exploring a neurogenic basis of velopharyngeal dysfunction in Tbx1 mutant mice: no difference in volumes of the nucleus ambiguus. INT J PEDIATR OTORHI 2013;77 (6):1002-1007 [PubMed]
Trowe MO, Zhao L, Weiss AC, Christoffels V, Epstein DJ, Kispert A, Inhibition of Sox2-dependent activation of Shh in the ventral diencephalon by Tbx3 is required for formation of the neurohypophysis. DEVELOPMENT 2013;140 (11):2299-2309 [PubMed]
van den Boogaard M, Wong LYE, Christoffels VM, Barnett P, Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation. METHODS MOL BIOL 2013;977:53-64 [PubMed]
Wong LYE. Genomic mapping of Tbx3 interactions in the heart. s.l.: s.n.; 2013. 187p. ISBN 9789461822376 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM, Christoffels VM; copromotor(s): Barnett P)
Bakker ML. Role of Tbx3 in conduction system development. s.l.: s.n.; 2012. 192p. ISBN 9789461909879 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Christoffels VM, Moorman AFM; copromotor(s): )
Bakker ML, Boink GJJ, Boukens BJ, Verkerk AO, van den Boogaard M, Den Haan AD, Hoogaars WMH, Buermans HP, de Bakker JMT, Seppen J, Tan HL, Moorman AFM, 't Hoen PAC, Christoffels VM, T-box transcription factor TBX3 reprogrammes mature cardiac myocytes into pacemaker-like cells. CARDIOVASC RES 2012;94 (3):439-449 [PubMed]
Barnett P, van den Boogaard M, Christoffels V, LOCALIZED AND TEMPORAL GENE REGULATION IN HEART DEVELOPMENT. CURR TOP DEV BIOL 2012;100:171-201 [PubMed]
Boukens BJ, Christoffels VM, Electrophysiological Patterning of the Heart. PEDIATR CARDIOL 2012;33 (6):900-906 [PubMed]
Boukens BJ, Christoffels VM, Popeye proteins: muscle for the aging sinus node. J CLIN INVEST 2012;122 (3):810-813 [PubMed]
Boukens BJD. Electrophysiological patterning of the heart. s.l.: s.n.; 2012. 167p. ISBN 9789461909978 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Christoffels VM; copromotor(s): Coronel R)
Frank DU, Carter KL, Thomas KR, Burr RM, Bakker ML, Coetzee WA, Tristani-Firouzi M, Bamshad MJ, Christoffels VM, Moon AM, Lethal arrhythmias in Tbx3-deficient mice reveal extreme dosage sensitivity of cardiac conduction system function and homeostasis. P NATL ACAD SCI USA 2012;109 (3):E154-E163 [PubMed]
Hyde AS, Farmer EL, Easley KE, van Lammeren K, Christoffels VM, Barycki JJ, Bakkers J, Simpson MA, UDP-glucose Dehydrogenase Polymorphisms from Patients with Congenital Heart Valve Defects Disrupt Enzyme Stability and Quaternary Assembly. J BIOL CHEM 2012;287 (39):32708-32716 [PubMed]
Jensen B, Boukens BJD, Postma AV, Gunst QD, van den Hoff MJB, Moorman AFM, Wang T, Christoffels VM, Identifying the Evolutionary Building Blocks of the Cardiac Conduction System. PLOS ONE 2012;7 (9):e44231 [PubMed]
Mesbah K, Rana MS, Francou A, van Duijvenboden K, Papaioannou VE, Moorman AF, Kelly RG, Christoffels VM, Identification of a Tbx1/Tbx2/Tbx3 genetic pathway governing pharyngeal and arterial pole morphogenesis. HUM MOL GENET 2012;21 (6):1217-1229 [PubMed]
Norden J, Grieskamp T, Christoffels VM, Moorman AFM, Kispert A, Partial Absence of Pleuropericardial Membranes in Tbx18- and Wt1-Deficient Mice. PLOS ONE 2012;7 (9):e45100 [PubMed]
Singh R, Hoogaars WM, Barnett P, Grieskamp T, Rana MS, Buermans H, Farin HF, Petry M, Heallen T, Martin JF, Moorman AFM, 't Hoen PAC, Kispert A, Christoffels VM, Tbx2 and Tbx3 induce atrioventricular myocardial development and endocardial cushion formation. CELL MOL LIFE SCI 2012;69 (8):1377-1389 [PubMed]
Tessadori F, van Weerd JH, Burkhard SB, Verkerk AO, de Pater E, Boukens BJ, Vink A, Christoffels VM, Bakkers J, Identification and Functional Characterization of Cardiac Pacemaker Cells in Zebrafish. PLOS ONE 2012;7 (10):e47644 [PubMed]
van den Boogaard M, Wong LYE, Tessadori F, Bakker ML, Dreizehnter LK, Wakker V, Bezzina CR, 't Hoen PAC, Bakkers J, Barnett P, Christoffels VM, Genetic variation in T-box binding element functionally affects SCN5A/SCN10A enhancer. J CLIN INVEST 2012;122 (7):2519-2530 [PubMed]
van Engelen K, Mommersteeg MTM, Baars MJH, Lam J, Ilgun A, van Trotsenburg ASP, Smets AMJB, Christoffels VM, Mulder BJM, Postma AV, The Ambiguous Role of NKX2-5 Mutations in Thyroid Dysgenesis. PLOS ONE 2012;7 (12):e52685 [PubMed]
Aanhaanen WTJ. The role of Tbx2 in the development of the atrioventriculair canal and conduction system to the heart. 'Making the beat go on and on'. s.l.: s.n.; 2011. 151p. ISBN 9789461910837 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM, Christoffels VM; copromotor(s): )
Aanhaanen WTJ, Boukens BJD, Sizarov A, Wakker V, de Gier-de Vries C, van Ginneken AC, Moorman AFM, Coronel R, Christoffels VM, Defective Tbx2-dependent patterning of the atrioventricular canal myocardium causes accessory pathway formation in mice. J CLIN INVEST 2011;121 (2):534-544 [PubMed]
Aanhaanen WTJ, Moorman AFM, Christoffels VM, Origin and development of the atrioventricular myocardial lineage: insight into the development of accessory pathways. BIRTH DEFECTS RES A 2011;91 (6):565-577 [PubMed]
Boogerd CJJ, Wong LYE, van den Boogaard M, Bakker ML, Tessadori F, Bakkers J, 't Hoen PAC, Moorman AF, Christoffels VM, Barnett P, Sox4 mediates Tbx3 transcriptional regulation of the gap junction protein Cx43. CELL MOL LIFE SCI 2011;68 (23):3949-3961 [PubMed]
Cai X, Nomura-Kitabayashi A, Cai W, Yan J, Christoffels VM, Cai CL, Myocardial Tbx20 regulates early atrioventricular canal formation and endocardial epithelial-mesenchymal transition via Bmp2. DEV BIOL 2011;360 (2):381-390 [PubMed]
Christoffels V, REGENERATIVE MEDICINE Muscle for a damaged heart. NATURE 2011;474 (7353):585-586 [PubMed]
Postma AV, Christoffels VM, Bezzina CR, Developmental aspects of cardiac arrhythmogenesis. CARDIOVASC RES 2011;91 (2):243-251 [PubMed]
Sizarov A, Devalla HD, Anderson RH, Passier R, Christoffels VM, Moorman AFM, Molecular analysis of patterning of conduction tissues in the developing human heart. CIRC-ARRHYTHMIA ELEC 2011;4 (4):532-542 [PubMed]
Sizarov A, Ya J, de Boer BA, Lamers WH, Christoffels VM, Moorman AFM, Formation of the Building Plan of the Human Heart Morphogenesis, Growth, and Differentiation. CIRCULATION 2011;123 (10):1125-U285 [PubMed]
Verhoeven MC, Haase C, Christoffels VM, Weidinger G, Bakkers J, Wnt signaling regulates atrioventricular canal formation upstream of BMP and Tbx2. BIRTH DEFECTS RES A 2011;91 (6):435-440 [PubMed]
Aanhaanen WTJ, Mommersteeg MTM, Norden J, Wakker V, de Gier-de Vries C, Anderson RH, Kispert A, Moorman AFM, Christoffels VM, Developmental Origin, Growth, and Three-Dimensional Architecture of the Atrioventricular Conduction Axis of the Mouse Heart. CIRC RES 2010;107 (6):728-U98 [PubMed]
Bakker ML, Christoffels VM, Moorman AFM, The Cardiac Pacemaker and Conduction System Develops From Embryonic Myocardium that Retains Its Primitive Phenotype. J CARDIOVASC PHARM 2010;56 (1):6-15 [PubMed]
Bakker ML, Moorman AFM, Christoffels VM, The atrioventricular node: origin, development, and genetic program. TRENDS CARDIOVAS MED 2010;20 (5):164-171 [PubMed]
Christoffels VM, Smits GJ, Kispert A, Moorman AFM, Development of the Pacemaker Tissues of the Heart. CIRC RES 2010;106 (2):240-254 [PubMed]
Lage K, Møllgård K, Greenway S, Wakimoto H, Gorham JM, Workman CT, Bendsen E, Hansen NT, Rigina O, Roque FS, Wiese C, Christoffels VM, Roberts AE, Smoot LB, Pu WT, Donahoe PK, Tommerup N, Brunak S, Seidman CE, Seidman JG, Larsen LA, Dissecting spatio-temporal protein networks driving human heart development and related disorders. MOL SYST BIOL 2010;6:381 [PubMed]
Mommersteeg MTM, Domínguez JN, Wiese C, Norden J, de Gier-de Vries C, Burch JBE, Kispert A, Brown NA, Moorman AFM, Christoffels VM, The sinus venosus progenitors separate and diversify from the first and second heart fields early in development. CARDIOVASC RES 2010;87 (1):92-101 [PubMed]
Norden J, Grieskamp T, Lausch E, van Wijk B, van den Hoff MJB, Englert C, Petry M, Mommersteeg MTM, Christoffels VM, Niederreither K, Kispert A, Wt1 and Retinoic Acid Signaling in the Subcoelomic Mesenchyme Control the Development of the Pleuropericardial Membranes and the Sinus Horns. CIRC RES 2010;106 (7):1212-U97 [PubMed]
Sizarov A, Anderson RH, Christoffels VM, Moorman AFM, Three-Dimensional and Molecular Analysis of the Venous Pole of the Developing Human Heart. CIRCULATION 2010;122 (8):798-U104 [PubMed]
Wakker V, Brons JF, Aanhaanen WTJ, van Roon MA, Moorman AFM, Christoffels VM, Generation of Mice With a Conditional Null Allele For Tbx2. GENESIS 2010;48 (3):195-199 [PubMed]
Aanhaanen WTJ, Brons JF, Domínguez JN, Rana MS, Norden J, Airik R, Wakker V, de Gier-de Vries C, Brown NA, Kispert A, Moorman AFM, Christoffels VM, The Tbx2+ primary myocardium of the atrioventricular canal forms the atrioventricular node and the base of the left ventricle. CIRC RES 2009;104 (11):1267-1274 [PubMed]
Boukens BJD, Christoffels VM, Coronel R, Moorman AFM, Developmental basis for electrophysiological heterogeneity in the ventricular and outflow tract myocardium as a substrate for life-threatening ventricular arrhythmias. CIRC RES 2009;104 (1):19-31 [PubMed]
Christoffels VM, Grieskamp T, Norden J, Mommersteeg MTM, Rudat C, Kispert A, Tbx18 and the fate of epicardial progenitors. NATURE 2009;458 (7240):E8-E9 [PubMed]
Christoffels VM, Moorman AFM, Development of the cardiac conduction system: why are some regions of the heart more arrhythmogenic than others?. CIRC-ARRHYTHMIA ELEC 2009;2 (2):195-207 [PubMed]
Horsthuis T. Regulation of the diversification of the nodal and chamber myocardium. s.l.: s.n.; 2009. 143p. ISBN 9789490122300 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Horsthuis T, Buermans HPJ, Brons JF, Verkerk AO, Bakker ML, Wakker V, Clout DEW, Moorman AFM, 't Hoen PAC, Christoffels VM, Gene expression profiling of the forming atrioventricular node using a novel tbx3-based node-specific transgenic reporter. CIRC RES 2009;105 (1):61-69 [PubMed]
Horsthuis T, Christoffels VM, Anderson RH, Moorman AFM, Can recent insights into cardiac development improve our understanding of congenitally malformed hearts?. CLIN ANAT 2009;22 (1):4-20 [PubMed]
Lüdtke THW, Christoffels VM, Petry M, Kispert A, Tbx3 Promotes Liver Bud Expansion During Mouse Development by Suppression of Cholangiocyte Differentiation. HEPATOLOGY 2009;49 (3):969-978 [PubMed]
Mommersteeg MTM. The development of the venous pole of the heart. s.l.: s.n.; 2009. 160p. ISBN 9789081451116 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Mommersteeg MTM, Christoffels VM, Anderson RH, Moorman AFM, Atrial fibrillation: A developmental point of view. HEART RHYTHM 2009;6 (12):1818-1824 [PubMed]
Remme CA, Verkerk AO, Hoogaars WMH, Aanhaanen WTJ, Scicluna BP, Annink C, van den Hoff MJB, Wilde AAM, van Veen TAB, Veldkamp MW, de Bakker JMT, Christoffels VM, Bezzina CR, The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium. BASIC RES CARDIOL 2009;104 (5):511-522 [PubMed]
Singh R, Horsthuis T, Farin HF, Grieskamp T, Norden J, Petry M, Wakker V, Moorman AFM, Christoffels VM, Kispert A, Tbx20 interacts with smads to confine tbx2 expression to the atrioventricular canal. CIRC RES 2009;105 (5):442-452 [PubMed]
Wiese C, Grieskamp T, Airik R, Mommersteeg MTM, Gardiwal A, de Gier-de Vries C, Schuster-Gossler K, Moorman AFM, Kispert A, Christoffels VM, Formation of the sinus node head and differentiation of sinus node myocardium are independently regulated by Tbx18 and Tbx3. CIRC RES 2009;104 (3):388-397 [PubMed]
Zirzow S, Lüdtke THW, Brons JF, Petry M, Christoffels VM, Kispert A, Expression and requirement of T-box transcription factors Tbx2 and Tbx3 during secondary palate development in the mouse. DEV BIOL 2009;336 (2):145-155 [PubMed]
Anderson RH, Boyett MR, Dobrzynski H, Yanni J, Christoffels VM, Moorman AFM, Letter by anderson et al regarding article, "abnormal conduction and morphology in the atrioventricular node of mice with atrioventricular canal-targeted deletion of Alk3/Bmpr1a receptor". CIRCULATION 2008;118 (6):e105; author reply e107 [PubMed]
Bakker ML, Boukens BJ, Mommersteeg MTM, Brons JF, Wakker V, Moorman AFM, Christoffels VM, Transcription factor Tbx3 is required for the specification of the atrioventricular conduction system. CIRC RES 2008;102 (11):1340-1349 [PubMed]
Boogerd KJ, Wong LYE, Christoffels VM, Klarenbeek M, Ruijter JM, Moorman AFM, Barnett P, Msx1 and Msx2 are functional interacting partners of T-box factors in the regulation of Connexin43. CARDIOVASC RES 2008;78 (3):485-493 [PubMed]
Hoogaars WMH, Barnett P, Rodriguez M, Clout DE, Moorman AFM, Goding CR, Christoffels VM, TBX3 and its splice variant TBX3 + exon 2a are functionally similar. PIGM CELL MELANOMA R 2008;21 (3):379-387 [PubMed]
Horsthuis T, Houweling AC, Habets PEMH, de Lange FJ, El Azzouzi H, Clout DEW, Moorman AFM, Christoffels VM, Distinct regulation of developmental and heart disease-induced atrial natriuretic factor expression by two separate distal sequences. CIRC RES 2008;102 (7):849-859 [PubMed]
Postma AV, Christoffels VM, Moorman AFM. Developmental aspects of the electrophysiology of the heart: function follows form in: Gussak I, Antzelevitch C, Wilde AAM, Friedman PA, Ackerman MJ, Shen WK, editors. Electrical diseases of the heart. Genetics, mechanisms, treatment, prevention.. London: Springer; 2008., p. 24-36
Postma AV, van de Meerakker JBA, Mathijssen IB, Barnett P, Christoffels VM, Ilgun A, Lam J, Wilde AAM, Lekanne Deprez RH, Moorman AFM, A gain-of-function TBX5 mutation is associated with atypical Holt-Oram syndrome and paroxysmal atrial fibrillation. CIRC RES 2008;102 (11):1433-1442 [PubMed]
de Lange FJ. Genetic lineage analysis of the developing heart. Universiteit van Amsterdam: s.n.; 2007. 184p. ISBN 90-9019156-9 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Hoogaars WMH. The role of Tbx3 in the formation of the cardiac conduction system. Universiteit van Amsterdam: s.n.; 2007. 202p. ISBN 9789090223193 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Hoogaars WMH, Barnett P, Moorman AFM, Christoffels VM, T-box factors determine cardiac design. CELL MOL LIFE SCI 2007;64 (6):646-660 [PubMed]
Hoogaars WMH, Christoffels VM, Moorman AFM. Morphogenesisiof the vertebrate heart in: Bodmer R, editors. Cardiovascular development. Amsterdam: Academic Press, Elsevier; 2008. 18, p. 31-68
Hoogaars WMH, Engel A, Brons JF, Verkerk AO, de Lange FJ, Wong LYE, Bakker ML, Clout DE, Wakker V, Barnett P, Ravesloot JH, Moorman AFM, Verheijck EE, Christoffels VM, Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria. GENE DEV 2007;21 (9):1098-1112 [PubMed]
Mommersteeg MTM, Brown NA, Prall OWJ, de Gier-de Vries C, Harvey RP, Moorman AFM, Christoffels VM, Pitx2c and Nkx2-5 are required for the formation and identity of the pulmonary myocardium. CIRC RES 2007;101 (9):902-909 [PubMed]
Mommersteeg MTM, Hoogaars WMH, Prall OWJ, de Gier-de Vries C, Wiese C, Clout DEW, Papaioannou VE, Brown NA, Harvey RP, Moorman AFM, Christoffels VM, Molecular pathway for the localized formation of the sinoatrial node. CIRC RES 2007;100 (3):354-362 [PubMed]
Moorman AFM, Christoffels VM, Anderson RH, van den Hoff MJB, The heart-forming fields: one or multiple?. PHILOS T R SOC B 2007;362 (1484):1257-1265 [PubMed]
Stoykov I, van Beeren HC, Moorman AFM, Christoffels VM, Wiersinga WM, Bakker O, Effect of amiodarone and dronedarone administration in rats on thyroid hormone-dependent gene expression in different cardiac components. EUR J ENDOCRINOL 2007;156 (6):695-702 [PubMed]
Christoffels VM, Mommersteeg MTM, Trowe MO, Prall OWJ, de Gier-de Vries C, Soufan AT, Bussen M, Schuster-Gossler K, Harvey RP, Moorman AFM, Kispert A, Formation of the venous pole of the heart from an Nkx2-5-negative precursor population requires Tbx18. CIRC RES 2006;98 (12):1555-1563 [PubMed]
Franco D, Meilhac SM, Christoffels VM, Kispert A, Buckingham M, Kelly RG, Left and right ventricular contributions to the formation of the interventricular septum in the mouse heart. DEV BIOL 2006;294 (2):366-375 [PubMed]
Houweling AC. Regulation of regionalized gene expression in the developing heart. S.l.: s.n.; 2006. 171p. ISBN 9789090205854 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Mommersteeg MTM, Soufan AT, de Lange FJ, van den Hoff MJB, Anderson RH, Christoffels VM, Moorman AFM, Two distinct pools of mesenchyme contribute to the development of the atrial septum. CIRC RES 2006;99 (4):351-353 [PubMed]
Stoykov I, Zandieh-Doulabi B, Moorman AFM, Christoffels V, Wiersinga WM, Bakker O, Expression pattern and ontogenesis of thyroid hormone receptor isoforms in the mouse heart. J ENDOCRINOL 2006;189 (2):231-245 [PubMed]
Fiore R, Rahim B, Christoffels VM, Moorman AFM, Püschel AW, Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system. MOL CELL BIOL 2005;25 (6):2310-2319 [PubMed]
Houweling AC, Somi S, Massink MPG, Groenen MA, Moorman AFM, Christoffels VM, Comparative analysis of the natriuretic peptide precursor gene cluster in vertebrates reveals loss of ANF and retention of CNP-3 in chicken. DEV DYNAM 2005;233 (3):1076-1082 [PubMed]
Houweling AC, van Borren MM, Moorman AFM, Christoffels VM, Expression and regulation of the atrial natriuretic factor encoding gene Nppa during development and disease. CARDIOVASC RES 2005;67 (4):583-593 [PubMed]
Moorman AFM, Christoffels VM. Concepts of cardiac development. Why some parts of the heart are more arrhythmogenic than other parts in: van Hemel NM, Vermeulen FEE, de Bakker JMT, editors. Exclusion or Targetting?. Nieuwegein: Grafische Producties Budde-Elinkwijk; 2005., p. 103-112
Moorman AFM, Christoffels VM, Anderson RH, Anatomic substrates for cardiac conduction. HEART RHYTHM 2005;2 (8):875-886 [PubMed]
Moorman AFM, de Boer PAJ, Small EM, Krieg PA, Christoffels VM. Evolutionary conservation of atrial natriureyic factor (Anf) expression, cardiac chamber formation, and the heart-forming region in: Artman M, Benson DW, Srivastava D, Nakazawa M, editors. Cardiovascular development and congenital malformations: molecular & genetic mechanisms. Maldem, MA: Blackwell Futura; 2005., p. 84-87
Singh MK, Christoffels VM, Dias JM, Trowe MO, Petry M, Schuster-Gossler K, Bürger A, Ericson J, Kispert A, Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2. DEVELOPMENT 2005;132 (12):2697-2707 [PubMed]
Anderson RH, Christoffels VM, Moorman AFM, Controversies concerning the anatomical definition of the conduction tissues. ANAT REC B NEW ANAT 2004;280 (1):8-14 [PubMed]
Christoffels VM, Burch JBE, Moorman AFM, Architectural plan for the heart: early patterning and delineation of the chambers and the nodes. TRENDS CARDIOVAS MED 2004;14 (8):301-307 [PubMed]
Christoffels VM, Hoogaars WMH, Tessari A, Clout DEW, Moorman AFM, Campione M, T-box transcription factor Tbx2 represses differentiation and formation of the cardiac chambers. DEV DYNAM 2004;229 (4):763-770 [PubMed]
de Lange FJ, Moorman AFM, Anderson RH, Männer J, Soufan AT, de Gier-de Vries C, Schneider MD, Webb S, van den Hoff MJB, Christoffels VM, Lineage and morphogenetic analysis of the cardiac valves. CIRC RES 2004;95 (6):645-654 [PubMed]
Hoogaars WMH, Tessari A, Moorman AFM, de Boer PAJ, Hagoort J, Soufan AT, Campione M, Christoffels VM, The transcriptional repressor Tbx3 delineates the developing central conduction system of the heart. CARDIOVASC RES 2004;62 (3):489-499 [PubMed]
Moorman AFM, Soufan AT, Hagoort J, de Boer PAJ, Christoffels VM, Development of the building plan of the heart. ANN NY ACAD SCI 2004;1015:171-181 [PubMed]
Streefkerk JO, Hoogaars WMH, Christoffels VM, Sand C, Pfaffendorf M, Peters SLM, van Zwieten PA, Vasopressin-induced vasoconstriction is dependent on MAPK(erk1/2) phosphorylation. FUND CLIN PHARMACOL 2004;18 (1):45-50 [PubMed]
de Lange FJ, Moorman AFM, Christoffels VM, Atrial cardiomyocyte-specific expression of Cre recombinase driven by an Nppa gene fragment. GENESIS 2003;37 (1):1-4 [PubMed]
Fijnvandraat AC, Lekanne Deprez RH, Christoffels VM, Ruijter JM, Moorman AFM, TBX5 overexpression stimulates differentiation of chamber myocardium in P19C16 embryonic carcinoma cells. J MUSCLE RES CELL M 2003;24 (2-3):211-218 [PubMed]
Fijnvandraat AC, van Ginneken ACG, de Boer PAJ, Ruijter JM, Christoffels VM, Moorman AFM, Lekanne Deprez RH, Cardiomyocytes derived from embryonic stem cells resemble cardiomyocytes of the embryonic heart tube. CARDIOVASC RES 2003;58 (2):399-409 [PubMed]
Fijnvandraat AC, van Ginneken ACG, Schumacher CA, Boheler KR, Lekanne Deprez RH, Christoffels VM, Moorman AFM, Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium. J MOL CELL CARDIOL 2003;35 (12):1461-1472 [PubMed]
Habets PEMH, Clout DEW, Lekanne Deprez RH, van Roon MA, Moorman AFM, Christoffels VM, Cardiac expression of Gal4 causes cardiomyopathy in a dose-dependent manner. J MUSCLE RES CELL M 2003;24 (2-3):205-209 [PubMed]
Habets PEMH, Moorman AFM, Christoffels VM. Expression systems to analyze transgenes in the heart in: Ince C, editor. The physiological genomics of the critically ill mouse. Amsterdam: Kluwer Academic Publishers; 2003., p. 201-210
Habets PEMH, Moorman AFM, Christoffels VM, Regulatory modules in the developing heart. CARDIOVASC RES 2003;58 (2):246-263 [PubMed]
Moorman AFM, Christoffels VM, Cardiac chamber formation: development, genes, and evolution. PHYSIOL REV 2003;83 (4):1223-1267 [PubMed]
Moorman AFM, Christoffels VM. Development of the cardiac conduction system: a matter of chamber development in: Chadwick DJ, Goode J, editors. Development of the cardiac conduction system. Chichester: John Wiley; 2003., p. 25-43
Moorman AFM, Christoffels VM, Development of the cardiac conduction system: a matter of chamber development. NOVART FDN SYMP 2003;250:25-34; discussion 34-43, 276-9 [PubMed]
Habets PEMH. The regulation of chamber-specific gene expression in the developing heart. S.l.: s.n.; 2002. 183p. ISBN 90-9015992-4 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Christoffels VM)
Habets PEMH, Moorman AFM, Clout DEW, van Roon MA, Lingbeek M, van Lohuizen M, Campione M, Christoffels VM, Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formation. GENE DEV 2002;16 (10):1234-1246 [PubMed]
Houweling AC, Somi S, van den Hoff MJB, Moorman AFM, Christoffels VM, Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken. ANAT REC 2002;266 (2):93-102 [PubMed]
Campione M, Ros MA, Icardo JM, Piedra E, Christoffels VM, Schweickert A, Blum M, Franco D, Moorman AF, Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. DEV BIOL 2001;231 (1):252-264 [PubMed]
Houweling AC, Dildrop R, Peters T, Mummenhoff J, Moorman AF, Rüther U, Christoffels VM, Gene and cluster-specific expression of the Iroquois family members during mouse development. MECH DEVELOP 2001;107 (1-2):169-174 [PubMed]
Moorman AF, Houweling AC, de Boer PA, Christoffels VM, Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol. J HISTOCHEM CYTOCHEM 2001;49 (1):1-8 [PubMed]
Mummenhoff J, Houweling AC, Peters T, Christoffels VM, Rüther U, Expression of Irx6 during mouse morphogenesis. MECH DEVELOP 2001;103 (1-2):193-195 [PubMed]
Christoffels VM, Habets PE, Das AT, Clout DE, van Roon MA, Moorman AF, Lamers WH, A single regulatory module of the carbamoylphosphate synthetase I gene executes its hepatic program of expression. J BIOL CHEM 2000;275 (51):40020-40027 [PubMed]
Christoffels VM, Habets PE, Franco D, Campione M, de Jong F, Lamers WH, Bao ZZ, Palmer S, Biben C, Harvey RP, Moorman AF, Chamber formation and morphogenesis in the developing mammalian heart. DEV BIOL 2000;223 (2):266-278 [PubMed]
Christoffels VM, Keijser AG, Houweling AC, Clout DE, Moorman AF, Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart. DEV BIOL 2000;224 (2):263-274 [PubMed]
Moorman AF, Schumacher CA, de Boer PA, Hagoort J, Bezstarosti K, van den Hoff MJ, Wagenaar GT, Lamers JM, Wuytack F, Christoffels VM, Fiolet JW, Presence of functional sarcoplasmic reticulum in the developing heart and its confinement to chamber myocardium. DEV BIOL 2000;223 (2):279-290 [PubMed]
Abdullah Abu Musa DM, Kobayashi K, Yasuda I, Iijima M, Christoffels VM, Tomomura M, Horiuchi M, Ohnishi T, Kajihara T, Daikuhara Y, Lamers WH, Saheki T, Involvement of a cis-acting element in the suppression of carbamoyl phosphate synthetase I gene expression in the liver of carnitine-deficient mice. MOL GENET METAB 1999;68 (3):346-356 [PubMed]
Christoffels VM, Sassi H, Ruijter JM, Moorman AF, Grange T, Lamers WH, A mechanistic model for the development and maintenance of portocentral gradients in gene expression in the liver. HEPATOLOGY 1999;29 (4):1180-1192 [PubMed]
Christoffels VM, Grange T, Kaestner KH, Cole TJ, Darlington GJ, Croniger CM, Lamers WH, Glucocorticoid receptor, C/EBP, HNF3, and protein kinase A coordinately activate the glucocorticoid response unit of the carbamoylphosphate synthetase I gene. MOL CELL BIOL 1998;18 (11):6305-6315 [PubMed]
Kimura T, Christoffels VM, Chowdhury S, Iwase K, Matsuzaki H, Mori M, Lamers WH, Darlington GJ, Takiguchi M, Hypoglycemia-associated hyperammonemia caused by impaired expression of ornithine cycle enzyme genes in C/EBPalpha knockout mice. J BIOL CHEM 1998;273 (42):27505-27510 [PubMed]
Christoffels VM, van den Hoff MJ, Lamers MC, van Roon MA, de Boer PA, Moorman AF, Lamers WH, The upstream regulatory region of the carbamoyl-phosphate synthetase I gene controls its tissue-specific, developmental, and hormonal regulation in vivo. J BIOL CHEM 1996;271 (49):31243-31250 [PubMed]
Christoffels VM, van den Hoff MJ, Moorman AF, Lamers WH, The far-upstream enhancer of the carbamoyl-phosphate synthetase I gene is responsible for the tissue specificity and hormone inducibility of its expression. J BIOL CHEM 1995;270 (42):24932-24940 [PubMed]
van den Hoff MJ, Christoffels VM, Labruyère WT, Moorman AF, Lamers WH, Electrotransfection with "intracellular" buffer. METHODS MOL BIOL 1995;48:185-197 [PubMed]
KOK RG, Christoffels VM, VOSMAN B, Hellingwerf KJ, A gene of Acinetobacter calcoaceticus BD413 encodes a periplasmic peptidyl-prolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth. BBA-GENE STRUCT EXPR 1994;1219 (3):601-606 [PubMed]
KOK RG, Christoffels VM, VOSMAN B, Hellingwerf KJ, Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases. J GEN MICROBIOL 1993;139 (10):2329-2342 [PubMed]
Key publications

van Duijvenboden K, de Boer BA, Capon N, Ruijter JM, Christoffels VM, EMERGE: a flexible modelling framework to predict genomic regulatory elements from genomic signatures. NUCLEIC ACIDS RES 2016;44 (5):e42 [PubMed]
Stefanovic S, Barnett P, van Duijvenboden K, Weber D, Gessler M, Christoffels VM, GATA-dependent regulatory switches establish atrioventricular canal specificity during heart development. NAT COMMUN 2014;5:3680 [PubMed]
van Weerd JH, Badi I, van den Boogaard M, Stefanovic S, van de Werken HJG, Gomez-Velazquez M, Badia-Careaga C, Manzanares M, de Laat W, Barnett P, Christoffels VM, A large permissive regulatory domain exclusively controls Tbx3 expression in the cardiac conduction system. CIRC RES 2014;115 (4):432-441 [PubMed]
Aanhaanen WTJ, Boukens BJD, Sizarov A, Wakker V, de Gier-de Vries C, van Ginneken AC, Moorman AFM, Coronel R, Christoffels VM, Defective Tbx2-dependent patterning of the atrioventricular canal myocardium causes accessory pathway formation in mice. J CLIN INVEST 2011;121 (2):534-544 [PubMed]
van den Boogaard M, Wong LYE, Tessadori F, Bakker ML, Dreizehnter LK, Wakker V, Bezzina CR, 't Hoen PAC, Bakkers J, Barnett P, Christoffels VM, Genetic variation in T-box binding element functionally affects SCN5A/SCN10A enhancer. J CLIN INVEST 2012;122 (7):2519-2530 [PubMed]


All Publications

Research programmes

Transcriptional mechanism controlling heart development, regeneration and rhythm

From 2001 I was taken on as a group leader to unravel the molecular basis of localized gene regulation and cell specification in the developing heart. In 2010 I was appointed professor of developmental biology in the department of Anatomy, Embryology & Physiology. My first breakthrough was the discovery that the location of development of the chambers and the atrioventricular canal in the heart tube is regulated by locally active T-box transcriptional repressors (Genes Dev. 2002). In 2005 I received an NWO VIDI grant to further study the role of T-box factors in the heart. A second breakthrough was the discovery that conduction system development is controlled by Tbx2 and Tbx3 (Genes Dev. 2007, Circ Res. 2008, PNAS 2012). Using genetic lineage analysis tools we addressed the contributions of different progenitor cells to the developing heart, and the role of T-box and other transcription factors in the formation of the pacemaker and conduction system components (Circ Res 2007-2010, Nature 2009). Our work on the development of the heart and conduction system has become internationally well recognized (Physiol Rev 2003, Circ Res. 2010). Recently, we uncovered a mechanism linking developmental defects of the heart and ventricular pre-excitation (JCI 2011), and, using 4C-seq and transgenesis, identified a common genetic variant in the population that disrupts T-box factor binding and sodium channel gene enhancer function in vivo (JCI 2012, Nat Genet 2013, JCI 2014). Furthermore, using in vivo ChIP-seq, we uncovered a transcriptional and epigenetic mechanism for the specification of the atrioventricular canal of the heart involving GATA transcription factors and tissue-specific histone (de)acetylation (Nat Commun). Genetic variants in the human genome influence cardiac development and heart rhythm/arrhythmia risk. Current research focuses on identifying the transcriptional mechanisms underlying this phenomenon. Furthermore, we are exploring the transcriptional mechanisms that (re)program the pacemaker and conduction system cells of the heart.

Theme: Cardiovascular Diseases

This research group participates in Heart Failure Research Center

Faculty
Prof. dr. V.M. Christoffels
Dr. P. Barnett
Dr. A.V. Postma

Postdocs
Dr. S. Ahuja
Dr. F.M. Bosada - Musselwhite PhD
Dr. J.A. Janssen
Dr. B.J. Jensen MSc (Aarhus University, Denmark)

PhD Students
Drs. K. van Duijvenboden
Drs. V.W.W. van Eif
M. Gunthel
Drs. I.B. Hooijkaas
Drs. J.C.K. Man (ICIN)
A. Mattiotti MSc
Drs. R.A. Mohan
A.F. van Ouwerkerk
S. Prakash MSc
K.T. Scholman
Drs. J.H. van Weerd

Others
C. de Gier-de Vries
Ing. V. Wakker

Other research related activities
  • None reported
Current research funding
  • AMC
  • AMC (Vrijgesteld)
  • Fondation Leducq
  • Hubrecht Inst. voor Ontwikkelings- biologie en Stamcelonderzoek
  • Nederlandse Hartstichting
  • TNO