• Principal Investigator
  • Male

Dr. M.J.B. van den Hoff

Position: Associate professor (UHD)
Main activities: Research, Teaching
Specialisation: cell and molecular biology, embryology, cardiac development
foto M.J.B. van den Hoff is one of the AMC Principal Investigators
Focus of research:

Cardiac development

Growth factor signalling

Cardiac malformations

AMC themes: Cardiovascular Diseases, Reproduction and development
Departments: Medical Biology
de Bakker BS, Driessen S, Boukens BJD, van den Hoff MJH, Oostra RJ, Single-site neural tube closure in human embryos revisited. CLIN ANAT 2017;30 (7):988-999 [PubMed]
Prakash S , Borreguero LJJ, Sylva M, Flores Ruiz L, Rezai F, Gunst QD, de la Pompa JL, Ruijter JM, van den Hoff MJB, Deletion of Fstl1 (Follistatin-Like 1) From the Endocardial/Endothelial Lineage Causes Mitral Valve Disease. ARTERIOSCL THROM VAS 2017;37 (9):E116-+ [PubMed]
Ruiz-Villalba A, Mattiotti A , Gunst QD, Cano-Ballesteros S, van den Hoff MJB, Ruijter JM, Reference genes for gene expression studies in the mouse heart. SCI REP-UK 2017;7 (1):24 [PubMed]
Tania NP, Maarsingh H, T Bos IS, Mattiotti A , Prakash S , Timens W, Gunst QD, Jimenez-Borreguero LJ, Schmidt M, van den Hoff MJB, Gosens R, Endothelial follistatin-like-1 regulates the postnatal development of the pulmonary vasculature by modulating BMP/Smad signaling. PULM CIRC 2017;7 (1):219-231 [PubMed]
Briggs LE, Burns TA, Lockhart MM, Phelps AL, van den Hoff MJB, Wessels A, Wnt/β-catenin and sonic hedgehog pathways interact in the regulation of the development of the dorsal mesenchymal protrusion. DEV DYNAM 2016;245 (2):103-113 [PubMed]
Hibender S, Franken R, van Roomen C, ter Braake A, van der Made I, Schermer EE, Gunst Q, van den Hoff MJ, Lutgens E, Pinto YM, Groenink M, Zwinderman AH, Mulder BJM, de Vries CJM, de Waard V, Resveratrol Inhibits Aortic Root Dilatation in the Fbn1C1039G/+ Marfan Mouse Model. ARTERIOSCL THROM VAS 2016;36 (8):1618-1626 [PubMed]
Lockhart MM, van den Hoff M, Wessels A. The Role of the Epicardium in the Formation of the Cardiac Valves in the Mouse in: Nakanishi T, Markwald RR, Baldwin HS, Keller BB, Srivastava D, Yamagishi H, editors. Etiology and Morphogenesis of Congenital Heart Disease: From Gene Function and Cellular Interaction to Morphology. S.l.: Springer Open; 2016., p. 161-170
Maruyama S, Nakamura K, Papanicolaou KN, Sano S, Shimizu I, Asaumi Y, van den Hoff MJ, Ouchi N, Recchia FA, Walsh K, Follistatin-like 1 promotes cardiac fibroblast activation and protects the heart from rupture. EMBO MOL MED 2016;8 (8):949-966 [PubMed]
Pérez-Pomares JM, de la Pompa JL, Franco D, Henderson D, Ho SY, Houyel L, Kelly RG, Sedmera D, Sheppard M, Sperling S, Thiene G, van den Hoff M, Basso C, Congenital coronary artery anomalies: a bridge from embryology to anatomy and pathophysiology--a position statement of the development, anatomy, and pathology ESC Working Group. CARDIOVASC RES 2016;109 (2):204-216 [PubMed]
Ruiz-Villalba A, Hoppler S, van den Hoff MJB, Wnt signaling in the heart fields: Variations on a common theme. DEV DYNAM 2016;245 (3):294-306 [PubMed]
Tanaka K, Valero-Muñoz M, Wilson RM, Essick EE, Fowler CT, Nakamura K, van den Hoff M, Ouchi N, Sam F, Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction. JACC BASIC TRANSL SCI 2016;1 (4):207-221 [PubMed]
Hayakawa S, Ohashi K, Shibata R, Kataoka Y, Miyabe M, Enomoto T, Joki Y, Shimizu Y, Kambara T, Uemura Y, Yuasa D, Ogawa H, Matsuo K, Hiramatsu-Ito M, van den Hoff MJB, Walsh K, Murohara T, Ouchi N, Cardiac myocyte-derived follistatin-like 1 prevents renal injury in a subtotal nephrectomy model. J AM SOC NEPHROL 2015;26 (3):636-646 [PubMed]
Ruijter JM, Ruiz Villalba A, Hellemans J, Untergasser A, van den Hoff MJB, Removal of between-run variation in a multi-plate qPCR experiment. BIOMOL DETECT QUANTIF 2015;5:10-14 [PubMed]
Wei K, Serpooshan V, Hurtado C, Diez-Cuñado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider MD, van den Hoff MJB, Butte MJ, Yang PC, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P, Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. NATURE 2015;525 (7570):479-485 [PubMed]
Briggs LE. Molecular and morphological assessment of second heart field derivatives involved in atrioventricular septation on the dorsal mesenchymal protrusion. s.l.: s.n.; 2014. 135p. Proefschrift Medical University of South Carolina, Regenerative Medicine and Cell Biology. (Promotor(s): Wessel A; copromotor(s): van den Hoff MJB)
Lockhart MM, Boukens BJD, Phelps AL, Brown CLM, Toomer KA, Burns TA, Mukherjee RD, Norris RA, Trusk TC, van den Hoff MJB, Wessels A, Alk3 mediated Bmp signaling controls the contribution of epicardially derived cells to the tissues of the atrioventricular junction. DEV BIOL 2014;396 (1):8-18 [PubMed]
Lockhart MM, Phelps AL, van den Hoff MJB, Wessels A, The Epicardium and the Development of the Atrioventricular Junction in the Murine Heart. J DEV BIOL 2014;2 (1):1-17 [PubMed]
Lockhart MM, Phelps AL, van den Hoff MJB, Wessels A, The Epicardium and the Development of the Atrioventricular Junction in the Murine Heart. J DEV BIOL 2014;2 (2):2221-3759
Miyabe M, Ohashi K, Shibata R, Uemura Y, Ogura Y, Yuasa D, Kambara T, Kataoka Y, Yamamoto T, Matsuo K, Joki Y, Enomoto T, Hayakawa S, Hiramatsu-Ito M, Ito M, van den Hoff MJB, Walsh K, Murohara T, Ouchi N, Muscle-derived follistatin-like 1 functions to reduce neointimal formation after vascular injury. CARDIOVASC RES 2014;103 (1):111-120 [PubMed]
Ruijter JM, Lorenz P, Tuomi JM, Hecker M, van den Hoff MJB, Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency. MICROCHIM ACTA 2014;181 (13-14):1689-1696 [PubMed]
Sylva M. On the connective tissue regulator Follistatin-like 1. S.l.: s.n.; 2014. 184p. ISBN 9789491602276 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): van den Hoff MJB)
Sylva M, van den Hoff MJB, Moorman AFM, Development of the human heart. AM J MED GENET A 2014;164A (6):1347-1371 [PubMed]
Briggs LE, Phelps AL, Brown E, Kakarla J, Anderson RH, van den Hoff MJB, Wessels A, Expression of the BMP receptor Alk3 in the second heart field is essential for development of the dorsal mesenchymal protrusion and atrioventricular septation. CIRC RES 2013;112 (11):1420-1432 [PubMed]
Sylva M, Moorman AFM, van den Hoff MJB, Follistatin-like 1 in vertebrate development. BIRTH DEFECTS RES C 2013;99 (1):61-69 [PubMed]
Velecela V, Lettice LA, Chau YY, Slight J, Berry RL, Thornburn A, Gunst QD, van den Hoff M, Reina M, Martínez FO, Hastie ND, Martínez-Estrada OM, WT1 regulates the expression of inhibitory chemokines during heart development. HUM MOL GENET 2013;22 (25):5083-5095 [PubMed]
de Boer BA, van den Berg G, Soufan AT, de Boer PAJ, Hagoort J, van den Hoff MJB, Moorman AFM, Ruijter JM, Measurement and 3D-Visualization of Cell-Cycle Length Using Double Labelling with Two Thymidine Analogues Applied in Early Heart Development. PLOS ONE 2012;7 (10):e47719 [PubMed]
Jensen B, Boukens BJD, Postma AV, Gunst QD, van den Hoff MJB, Moorman AFM, Wang T, Christoffels VM, Identifying the Evolutionary Building Blocks of the Cardiac Conduction System. PLOS ONE 2012;7 (9):e44231 [PubMed]
van Oppenraaij RHF, Koning AHJ, van den Hoff MJB, van der Spek PJ, Steegers EAP, Exalto N, The effect of smoking on early chorionic villous vascularisation. PLACENTA 2012;33 (8):645-651 [PubMed]
van Wijk B, Gunst QD, Moorman AFM, van den Hoff MJB, Cardiac Regeneration from Activated Epicardium. PLOS ONE 2012;7 (9):e44692 [PubMed]
Wessels A, van den Hoff MJB, Adamo RF, Phelps AL, Lockhart MM, Sauls K, Briggs LE, Norris RA, van Wijk B, Perez-Pomares JM, Dettman RW, Burch JBE, Epicardially derived fibroblasts preferentially contribute to the parietal leaflets of the atrioventricular valves in the murine heart. DEV BIOL 2012;366 (2):111-124 [PubMed]
Barnett P, van den Hoff MJB, Cardiac regeneration: different cells same goal. MED BIOL ENG COMPUT 2011;49 (7):723-732 [PubMed]
de Boer BA, Soufan AT, Hagoort J, Mohun TJ, van den Hoff MJB, Hasman A, Voorbraak FPJM, Moorman AFM, Ruijter JM, The interactive presentation of 3D information obtained from reconstructed datasets and 3D placement of single histological sections with the 3D portable document format. DEVELOPMENT 2011;138 (1):159-167 [PubMed]
Shimano M, Ouchi N, Nakamura K, van Wijk B, Ohashi K, Asaumi Y, Higuchi A, Pimentel DR, Sam F, Murohara T, van den Hoff MJB, Walsh K, Cardiac myocyte follistatin-like 1 functions to attenuate hypertrophy following pressure overload. P NATL ACAD SCI USA 2011;108 (43):E899-E906 [PubMed]
Sylva M, Li VSW, Buffing AAA, van Es JH, van den Born M, van der Velden S, Gunst Q, Koolstra JH, Moorman AFM, Clevers H, van den Hoff MJB, The BMP antagonist follistatin-like 1 is required for skeletal and lung organogenesis. PLOS ONE 2011;6 (8):e22616 [PubMed]
Buermans HPJ, van Wijk B, Hulsker MA, Smit NCH, den Dunnen JT, van Ommen GB, Moorman AF, van den Hoff MJ, 't Hoen PAC, Comprehensive Gene-Expression Survey Identifies Wif1 as a Modulator of Cardiomyocyte Differentiation. PLOS ONE 2010;5 (12):e15504 [PubMed]
Norden J, Grieskamp T, Lausch E, van Wijk B, van den Hoff MJB, Englert C, Petry M, Mommersteeg MTM, Christoffels VM, Niederreither K, Kispert A, Wt1 and Retinoic Acid Signaling in the Subcoelomic Mesenchyme Control the Development of the Pleuropericardial Membranes and the Sinus Horns. CIRC RES 2010;106 (7):1212-U97 [PubMed]
van Wijk A. The myocardial potential of proepicardial cells. From development to cardiac regeneration. s.l.: s.n.; 2010. 216p. ISBN 9789461081117 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): van den Hoff MJB)
van Wijk B, van den Hoff M, Epicardium and myocardium originate from a common cardiogenic precursor pool. TRENDS CARDIOVAS MED 2010;20 (1):1-7 [PubMed]
Campian ME, Verberne HJ, Hardziyenka M, de Bruin K, Selwaness M, van den Hoff MJB, Ruijter JM, van Eck-Smit BLF, de Bakker JMT, Tan HL, Serial Noninvasive Assessment of Apoptosis During Right Ventricular Disease Progression in Rats. J NUCL MED 2009;50 (8):1371-1377 [PubMed]
Pilichou K, Remme CA, Basso C, Campian ME, Rizzo S, Barnett P, Scicluna BP, Bauce B, van den Hoff MJB, de Bakker JMT, Tan HL, Valente M, Nava A, Wilde AAM, Moorman AFM, Thiene G, Bezzina CR, Myocyte necrosis underlies progressive myocardial dystrophy in mouse dsg2-related arrhythmogenic right ventricular cardiomyopathy. J EXP MED 2009;206 (8):1787-1802 [PubMed]
Remme CA, Verkerk AO, Hoogaars WMH, Aanhaanen WTJ, Scicluna BP, Annink C, van den Hoff MJB, Wilde AAM, van Veen TAB, Veldkamp MW, de Bakker JMT, Christoffels VM, Bezzina CR, The cardiac sodium channel displays differential distribution in the conduction system and transmural heterogeneity in the murine ventricular myocardium. BASIC RES CARDIOL 2009;104 (5):511-522 [PubMed]
Ruijter JM, Ramakers C, Hoogaars WMH, Karlen Y, Bakker O, van den Hoff MJB, Moorman AFM, Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. NUCLEIC ACIDS RES 2009;37 (6):e45 [PubMed]
van den Berg G, Abu-Issa R, de Boer BA, Hutson MR, de Boer PAJ, Soufan AT, Ruijter JM, Kirby ML, van den Hoff MJB, Moorman AFM, A caudal proliferating growth center contributes to both poles of the forming heart tube. CIRC RES 2009;104 (2):179-188 [PubMed]
van Oppenraaij RHF, Koning AHJ, Lisman BA, Boer K, van den Hoff MJB, van der Spek PJ, Steegers EAP, Exalto N, Vasculogenesis and Angiogenesis in the First Trimester Human Placenta: An Innovative 3D Study Using an Immersive Virtual Reality System. PLACENTA 2009;30 (3):220-222 [PubMed]
van Wijk B, van den Berg G, Abu-Issa R, Barnett P, van der Velden S, Schmidt M, Ruijter JM, Kirby ML, Moorman AFM, van den Hoff MJB, Epicardium and Myocardium Separate From a Common Precursor Pool by Crosstalk Between Bone Morphogenetic Protein- and Fibroblast Growth Factor-Signaling Pathways. CIRC RES 2009;105 (5):431-U71 [PubMed]
Barendrecht MM, Mulders ACM, van der Poel H, van den Hoff MJB, Schmidt M, Michel MC, Role of transforming growth factor beta in rat bladder smooth muscle cell proliferation. J PHARMACOL EXP THER 2007;322 (1):117-122 [PubMed]
Barnett P, Moorman AFM, van den Hoff MJB. Origins of cardiac differentiation and morphogenesis in: Wollert KC, Field LJ, editors. Rebuilding the infarcted heart. London: Informa Healthcare; 2007., p. 1-12
Lisman BAM, van den Hoff MJB, Boer K, Bleker OP, van Groningen K, Exalto N, The architecture of first trimester chorionic villous vascularization: a confocal laser scanning microscopical study. HUM REPROD 2007;22 (8):2254-2260 [PubMed]
Moorman AFM, Christoffels VM, Anderson RH, van den Hoff MJB, The heart-forming fields: one or multiple?. PHILOS T R SOC B 2007;362 (1484):1257-1265 [PubMed]
Rana MS, Horsten NCA, Tesink-Taekema S, Lamers WH, Moorman AFM, van den Hoff MJB, Trabeculated right ventricular free wall in the chicken heart forms by ventricularization of the myocardium initially forming the outflow tract. CIRC RES 2007;100 (7):1000-1007 [PubMed]
Soufan AT, van den Berg G, Moerland PD, Massink MMG, van den Hoff MJB, Moorman AFM, Ruijter JM, Three-dimensional measurement and visualization of morphogenesis applied to cardiac embryology. J MICROSC-OXFORD 2007;225 (Part 3):269-274 [PubMed]
van den Berg G, Somi S, Buffing AAM, Moorman AFM, van den Hoff MJB, Patterns of expression of the Follistatin and Follistatin-like1 genes during chicken heart development: a potential role in valvulogenesis and late heart muscle cell formation. ANAT REC 2007;290 (7):783-787 [PubMed]
van Wijk B, Moorman AFM, van den Hoff MJB, Role of bone morphogenetic proteins in cardiac differentiation. CARDIOVASC RES 2007;74 (2):244-255 [PubMed]
Kruithof BPT, van Wijk B, Somi S, Kruithof-de Julio M, Pérez Pomares JM, Weesie F, Wessels A, Moorman AFM, van den Hoff MJB, BMP and FGF regulate the differentiation of multipotential pericardial mesoderm into the myocardial or epicardial lineage. DEV BIOL 2006;295 (2):507-522 [PubMed]
Mommersteeg MTM, Soufan AT, de Lange FJ, van den Hoff MJB, Anderson RH, Christoffels VM, Moorman AFM, Two distinct pools of mesenchyme contribute to the development of the atrial septum. CIRC RES 2006;99 (4):351-353 [PubMed]
Moorman AFM, van den Hoff MJB, Heartrepair legt fundament onder stamcelonderzoek bij hartschade. HEALTHDIRECT CARDIOL 2006;2:9-12
Moralez I, Phelps A, Riley B, Raines M, Wirrig E, Snarr B, Jin JP, van den Hoff M, Hoffman S, Wessels A, Muscularizing tissues in the endocardial cushions of the avian heart are characterized by the expression of h1-calponin. DEV DYNAM 2006;235 (6):1648-1658 [PubMed]
Somi S, Klein ATJ, Houweling AC, Ruijter JM, Buffing AAM, Moorman AFM, van den Hoff MJB, Atrial and ventricular myosin heavy-chain expression in the developing chicken heart: strengths and limitations of non-radioactive in situ hybridization. J HISTOCHEM CYTOCHEM 2006;54 (6):649-664 [PubMed]
Soufan AT. Three dimensional visualization & analysis of cardiac development. S.l.: s.n.; 2006. 95p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): Ruijter JM, van den Hoff MJB)
Soufan AT, van den Berg G, Ruijter JM, de Boer PAJ, van den Hoff MJB, Moorman AFM, Regionalized sequence of myocardial cell growth and proliferation characterizes early chamber formation. CIRC RES 2006;99 (5):545-552 [PubMed]
van den Hoff MJB, Postma AV, Michel MC, Candidate genes for the hereditary component of cardiac hypertrophy. J HYPERTENS 2006;24 (2):273-277 [PubMed]
Schimmel K, Bennink R, de Bruin K, Leen R, Sand K, van den Hoff M, van Kuilenburg A, Vanderheyden JL, Steinmetz N, Pfaffendorf M, Verschuur A, Guchelaar HJ, Absence of cardiotoxicity of the experimental cytotoxic drug cyclopentenyl cytosine (CPEC) in rats. ARCH TOXICOL 2005;79 (5):268-276 [PubMed]
Somi S. Bone morphogenetica proteins in late heart muscle cell formation. S.l.: s.n.; 2005. 208p. ISBN 90-9018218-7 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): van den Hoff MJB)
van den Hoff MJB, Kruithof BPT, Wessels A, Markwald RR, Moorman AFM. Regulation of myocardium formation after the initial development of the linear heart tube in: Artman M, Benson DW, Srivastava D, Nakazawa M, editors. Cardiovascular development and congenital malformations: molecular & genetic mechanisms. Malden, MA: Blackwell Futura; 2005., p. 37-40
van den Hoff MJB, Moorman AFM, Wnt, a driver of myocardialization?. CIRC RES 2005;96 (3):274-276 [PubMed]
Bennink RJ, van den Hoff MJ, van Hemert FJ, de Bruin KM, Spijkerboer AL, Vanderheyden JL, Steinmetz N, van Eck-Smit BL, Annexin V imaging of acute doxorubicin cardiotoxicity (apoptosis) in rats. J NUCL MED 2004;45 (5):842-848 [PubMed]
de Lange FJ, Moorman AFM, Anderson RH, Männer J, Soufan AT, de Gier-de Vries C, Schneider MD, Webb S, van den Hoff MJB, Christoffels VM, Lineage and morphogenetic analysis of the cardiac valves. CIRC RES 2004;95 (6):645-654 [PubMed]
Somi S, Buffing AAM, Moorman AFM, van den Hoff MJB, Dynamic patterns of expression of BMP isoforms 2, 4, 5, 6, and 7 during chicken heart development. ANAT REC PART A 2004;279 (1):636-651 [PubMed]
Somi S, Buffing AAM, Moorman AFM, van den Hoff MJB, Expression of bone morphogenetic protein-10 mRNA during chicken heart development. ANAT REC PART A 2004;279 (1):579-582 [PubMed]
Soufan AT, van den Hoff MJB, Ruijter JM, de Boer PAJ, Hagoort J, Webb S, Anderson RH, Moorman AFM, Reconstruction of the patterns of gene expression in the developing mouse heart reveals an architectural arrangement that facilitates the understanding of atrial malformations and arrhythmias. CIRC RES 2004;95 (12):1207-1215 [PubMed]
van den Hoff MJB, Deprez RHL, Ruijter JM, de Boer PAJ, Tesink-Taekema S, Buffing AA, Lamers WH, Moorman AFM, Increased cardiac workload by closure of the ductus arteriosus leads to hypertrophy and apoptosis rather than to hyperplasia in the late fetal period. N-S ARCH PHARMACOL 2004;370 (3):193-202 [PubMed]
van den Hoff MJB, Kruithof BPT, Moorman AFM, Making more heart muscle. BIOESSAYS 2004;26 (3):248-261 [PubMed]
Kruithof BPT. Myocardium formation after the initial development of the linear heart tube. S.l.: s.n.; 2003. 185p. ISBN 90-9016024-8 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Moorman AFM; copromotor(s): van den Hoff MJB)
Kruithof BPT, van den Hoff MJB, Tesink-Taekema S, Moorman AFM, Recruitment of intra- and extracardiac cells into the myocardial lineage during mouse development. ANAT REC PART A 2003;271 (2):303-314 [PubMed]
Kruithof BPT, van den Hoff MJB, Wessels A, Moorman AFM, Cardiac muscle cell formation after development of the linear heart tube. DEV DYNAM 2003;227 (1):1-13 [PubMed]
Lombardi MP, van den Hoff MJB, Ruijter JM, Luijerink M, Buffing AA, Markman MW, Moorman AFM, Lekanne Deprez RH, Expression analysis of subtractively enriched libraries (EASEL): a widely applicable approach to the identification of differentially expressed genes. J BIOCHEM BIOPH METH 2003;57 (1):17-33 [PubMed]
Somi S, Houweling AC, Buffing AAM, Moorman AFM, van den Hoff MJB, Expression of cVg1 mRNA during chicken embryonic development. ANAT REC PART A 2003;273 (1):603-608 [PubMed]
Soufan AT, Ruijter JM, van den Hoff MJB, de Boer PAJ, Hagoort J, Moorman AFM, Three-dimensional reconstruction of gene expression patterns during cardiac development. PHYSIOL GENOMICS 2003;13 (3):187-195 [PubMed]
Houweling AC, Somi S, van den Hoff MJB, Moorman AFM, Christoffels VM, Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken. ANAT REC 2002;266 (2):93-102 [PubMed]
Demolombe S, Lande G, Charpentier F, van Roon MA, van den Hoff MJ, Toumaniantz G, Baro I, Guihard G, Le Berre N, Corbier A, de Bakker J, Opthof T, Wilde A, Moorman AF, Escande D, Transgenic mice overexpressing human KvLQT1 dominant-negative isoform. Part I: Phenotypic characterisation. CARDIOVASC RES 2001;50 (2):314-327 [PubMed]
Remme CA, Lombardi MP, van den Hoff MJ, Lekanne dit Deprez RH, CDNA arrays: the ups and downs. CARDIOVASC DRUG THER 2001;15 (2):99-101 [PubMed]
Spijkers JA, van den Hoff MJ, Hakvoort TB, Vermeulen JL, Tesink-Taekema S, Lamers WH, Foetal rise in hepatic enzymes follows decline in c-met and hepatocyte growth factor expression. J HEPATOL 2001;34 (5):699-710 [PubMed]
van den Eijnde SM, van den Hoff MJ, Reutelingsperger CP, van Heerde WL, Henfling ME, Vermeij-Keers C, Schutte B, Borgers M, Ramaekers FC, Transient expression of phosphatidylserine at cell-cell contact areas is required for myotube formation. J CELL SCI 2001;114 (20):3631-3642 [PubMed]
van den Hoff MJ, Kruithof BP, Moorman AF, Markwald RR, Wessels A, Formation of myocardium after the initial development of the linear heart tube. DEV BIOL 2001;240 (1):61-76 [PubMed]
Moorman AF, Schumacher CA, de Boer PA, Hagoort J, Bezstarosti K, van den Hoff MJ, Wagenaar GT, Lamers JM, Wuytack F, Christoffels VM, Fiolet JW, Presence of functional sarcoplasmic reticulum in the developing heart and its confinement to chamber myocardium. DEV BIOL 2000;223 (2):279-290 [PubMed]
van den Hoff MJ, Moorman AF, Cardiac neural crest: the holy grail of cardiac abnormalities?. CARDIOVASC RES 2000;47 (2):212-216 [PubMed]
van den Hoff MJ, van den Eijnde SM, Virágh S, Moorman AF, Programmed cell death in the developing heart. CARDIOVASC RES 2000;45 (3):603-620 [PubMed]
Mohammad-Panah R, Demolombe S, Neyroud N, Guicheney P, Kyndt F, van den Hoff M, Baró I, Escande D, Mutations in a dominant-negative isoform correlate with phenotype in inherited cardiac arrhythmias. AM J HUM GENET 1999;64 (4):1015-1023 [PubMed]
van den Hoff MJ, Moorman AF, Measure is treasure. CARDIOVASC RES 1999;43 (2):288-290 [PubMed]
van den Hoff MJ, Moorman AF, Ruijter JM, Lamers WH, Bennington RW, Markwald RR, Wessels A [Contributors: Markwald RR]] , Myocardialization of the cardiac outflow tract. DEV BIOL 1999;212 (2):477-490 [PubMed]
Korver W, Schilham MW, Moerer P, van den Hoff MJ, Dam K, Lamers WH, Medema RH, Clevers H, Uncoupling of S phase and mitosis in cardiomyocytes and hepatocytes lacking the winged-helix transcription factor Trident. CURR BIOL 1998;8 (24):1327-1330 [PubMed]
LekanneDeprez RH, van den Hoff MJ, de Boer PA, Ruijter PM, Maas AA, Chamuleau RA, Lamers WH, Moorman AF, Changing patterns of gene expression in the pulmonary trunk-banded rat heart. J MOL CELL CARDIOL 1998;30 (9):1877-1888 [PubMed]
Ya J, van den Hoff MJ, de Boer PA, Tesink-Taekema S, Franco D, Moorman AF, Lamers WH, Normal development of the outflow tract in the rat. CIRC RES 1998;82 (4):464-472 [PubMed]
Drewnowska K, Labruyere WT, van den Hoff MJ, Lamers WH, Schoolwerth AC, Stimulation of phosphoenolpyruvate carboxykinase gene expression in cultured LLC-PK1-F+ cells. CONTRIB NEPHROL 1997;121:25-30 [PubMed]
Jonker A, de Boer PA, van den Hoff MJ, Lamers WH, Moorman AF, Towards quantitative in situ hybridization. J HISTOCHEM CYTOCHEM 1997;45 (3):413-423 [PubMed]
van den Hoff MJ, Deprez RH, Monteiro M, de Boer PA, Charles R, Moorman AF, Developmental changes in rat cardiac DNA, RNA and protein tissue base: implications for the interpretation of changes in gene expression. J MOL CELL CARDIOL 1997;29 (2):629-639 [PubMed]
Christoffels VM, van den Hoff MJ, Lamers MC, van Roon MA, de Boer PA, Moorman AF, Lamers WH, The upstream regulatory region of the carbamoyl-phosphate synthetase I gene controls its tissue-specific, developmental, and hormonal regulation in vivo. J BIOL CHEM 1996;271 (49):31243-31250 [PubMed]
Christoffels VM, van den Hoff MJ, Moorman AF, Lamers WH, The far-upstream enhancer of the carbamoyl-phosphate synthetase I gene is responsible for the tissue specificity and hormone inducibility of its expression. J BIOL CHEM 1995;270 (42):24932-24940 [PubMed]
van den Hoff MJ, Christoffels VM, Labruyère WT, Moorman AF, Lamers WH, Electrotransfection with "intracellular" buffer. METHODS MOL BIOL 1995;48:185-197 [PubMed]
van den Hoff MJ, Jonker A, Beintema JJ, Lamers WH, Evolutionary relationships of the carbamoylphosphate synthetase genes. J MOL EVOL 1995;41 (6):813-832 [PubMed]
van den Hoff MJ, van de Zande LP, Dingemanse MA, Das AT, Labruyère W, Moorman AF, Charles R, Lamers WH, Isolation and characterization of the rat gene for carbamoylphosphate synthetase I. EUR J BIOCHEM 1995;228 (2):351-361 [PubMed]
van den Hoff MJ, Vermeulen JL, de Boer PA, Lamers WH, Moorman AF, Developmental changes in the expression of the liver-enriched transcription factors LF-B1, C/EBP, DBP and LAP/LIP in relation to the expression of albumin, alpha-fetoprotein, carbamoylphosphate synthase and lactase mRNA. HISTOCHEM J 1994;26 (1):20-31 [PubMed]
van den Hoff MJ, Labruyère WT, Moorman AF, Lamers WH, Mammalian gene expression is improved by use of a longer SV40 early polyadenylation cassette. NUCLEIC ACIDS RES 1993;21 (21):4987-4988 [PubMed]
van den Hoff MJ, Moorman AF, Lamers WH, Electroporation in 'intracellular' buffer increases cell survival. NUCLEIC ACIDS RES 1992;20 (11):2902 [PubMed]
Moorman AF, van den Hoff MJ, de Boer PA, Charles R, Lamers WH, The dynamics of the expression of C/EBP mRNA in the adult rat liver lobulus qualifies it as a pericentral mRNA. FEBS LETT 1991;288 (1-2):133-137 [PubMed]
van den Hoff MJ, Geerts WJ, Das AT, Moorman AF, Lamers WH, cDNA sequence of the long mRNA for human glutamine synthase. BBA-GENE STRUCT EXPR 1991;1090 (2):249-251 [PubMed]
van den Hoff MJ, Labruyère WT, Moorman AF, Lamers WH, The osmolarity of the electroporation medium affects the transient expression of genes. NUCLEIC ACIDS RES 1990;18 (21):6464 [PubMed]
Key publications

Ruiz-Villalba A, Hoppler S, van den Hoff MJB, Wnt signaling in the heart fields: Variations on a common theme. DEV DYNAM 2016;245 (3):294-306 [PubMed]
Ruijter JM, Lorenz P, Tuomi JM, Hecker M, van den Hoff MJB, Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency. MICROCHIM ACTA 2014;181 (13-14):1689-1696 [PubMed]
Sylva M, Moorman AFM, van den Hoff MJB, Follistatin-like 1 in vertebrate development. BIRTH DEFECTS RES C 2013;99 (1):61-69 [PubMed]
van Wijk B, Gunst QD, Moorman AFM, van den Hoff MJB, Cardiac Regeneration from Activated Epicardium. PLOS ONE 2012;7 (9):e44692 [PubMed]
Sylva M, Li VSW, Buffing AAA, van Es JH, van den Born M, van der Velden S, Gunst Q, Koolstra JH, Moorman AFM, Clevers H, van den Hoff MJB, The BMP antagonist follistatin-like 1 is required for skeletal and lung organogenesis. PLOS ONE 2011;6 (8):e22616 [PubMed]


All Publications

Research programmes

Heart muscle cell formation

The research line heart muscle cell formation focuses on heart muscle cell formation after development of the linear heart tube. A 3-dimensional gel lattice in vitro explant system has been developed in which heart muscle and epicardial development can be mimicked, manipulated and analyzed using confocal laser scanning microscopy. In vitro and in vivo heart development is influenced by applying growth factors and/or using recombinant adenoviruses, which are produced within the group, in the pericardial cavity. An extensive collection of constitutive active and dominant negative acting components of the BMP signaling pathway have been developed. The influences on heart development are analyzed using non-radioactive in situ hybridization, immunohistochemistry and quantitative 3D automated reconstruction methods. Heart muscle formation by myocardialization in the outflow tract is analyzed using a combined PCR-based subtraction and array method and heart muscle cell formation at the level of the proepicardium is investigated using in vitro arrays and microarrays.
Currently research focuses on heart muscle cell formation at the inflow of the heart, at the level of the proepicardium. Candidate gene analysis have identified an integrated role of BMP and FGF signalling, which is being pursued. An extensive genome wide analysis using chicken microarrays has been initiated to identify genes involved in heart muscle cell formation. The initial genome wide analysis has identified a potential regulator of myocardialization of which a global and conditional knock-out mouse has been made and is being analyzed.
 

Faculty
Dr. M.J.B. van den Hoff

Postdocs
Dr. A. Ruiz-Villalba

PhD Students
Drs. B.S. de Bakker MD
Drs. I.B. Hooijkaas
A. Mattiotti MSc
S. Prakash MSc
N.P. Tania MSc (University of Groningen)

Others
Ing. Q.D. Gunst

Other research related activities
  • None reported
Current research funding
  • AMC
  • Crediteurenadm. VUmc, p/a Profource Service Center