• Male

Dr. ir. E. van der Pol

Position: Postdoc
Main activities: Research
Specialisation: Detection of exosomes, microparticles and other extracellular vesicles as biomarkers for disease
foto
Focus of research:

Background. Clinical decisions for diagnosis, prognosis, and therapy are often based on information from biochemical and cellular analysis of blood and urine. It is becoming increasingly clear that these body fluids also contain numerous small (<0.001 mm) extracellular vesicles, often referred to as exosomes or microvesicles. Vesicles originate from a variety of cells and functions of vesicles include intercellular communication, coagulation, and inflammation. The vesicle profile (size, concentration, biochemical composition, and cellular origin) and function are altered in e.g. cancer and cardiovascular disease. Accordingly, the ability to measure this vesicle profile strongly adds to understanding the development of major diseases and provides a way for early detection of these diseases.

Problem. Although the clinical relevance of vesicles is increasingly recognized, the detection of exosomes, microparticles, and other extracellular vesicles is still cumbersome. Due to their small size and low refractive index vesicles are below the detection range of modern techniques. Consequently, worldwide no device is available that can determine the vesicle profile directly in body fluids to obtain clinical information.

Goal. The goal of my research is to develop a device that is suitable for clinical use and capable of accurately measuring the size, morphology (shape and ultrastructure), biochemical composition (proteins, lipids, RNA, DNA), and cellular origin of single vesicles at a rate of thousands per second.

For more information and a complete list of peer-reviewed articles to which I contributed, please visit www.edwinvanderpol.com.

AMC themes: Other research
Departments: Biomedical Engineering and Physics, Clinical Chemistry: Laboratory for Experimental Clinical Chemistry
Coumans FAW, Brisson AR, Buzas EI, Dignat-George F, Drees EEE, El-Andaloussi S, Emanueli C, Gasecka A, Hendrix A, Hill AF, Lacroix R, Lee Y, van Leeuwen TG, Mackman N, Mäger I, Nolan JP, van der Pol E, Pegtel DM, Sahoo S, Siljander PRM, Sturk G, de Wever O, Nieuwland R, Methodological Guidelines to Study Extracellular Vesicles. CIRC RES 2017;120 (10):1632-1648 [PubMed]
Gasecka A, van der Pol E, Nieuwland R, Stępień E, Extracellular vesicles in post-infarct ventricular remodelling. KARDIOL POL 2017;ahead of print [PubMed]
Valkonen S, van der Pol E, Böing A, Yuana Y, Yliperttula M, Nieuwland R, Laitinen S, Siljander PRM, Biological reference materials for extracellular vesicle studies. EUR J PHARM SCI 2017;98:4-16 [PubMed]
van der Pol E, Harrison P, From platelet dust to gold dust: physiological importance and detection of platelet microvesicles. PLATELETS 2017;28 (3):211-213 [PubMed]
Cernohorsky P, Jansen SM, de Bruin DM, van der Pol E, Savci-Heijink CD, Strackee SD, Faber DJ, van Leeuwen TG, Quantitative assessment of optical properties in healthy cartilage and repair tissue by optical coherence tomography and histology.. IEEE J SEL TOP QUANT 2016;22 (3):6801407
Nicolet A, Meli F, van der Pol E, Yuana Y, Gollwitzer C, Krumrey M, Cizmar P, Buhr E, Petry J, Sebaihi N, de Boeck B, Fokkema V, Bergmans R, Nieuwland R, Inter-laboratory comparison on the size and stability of monodisperse and bimodal synthetic reference particles for standardization of extracellular vesicle measurements. MEAS SCI TECHNOL 2016;27 (3):035701
van der Pol E, Böing AN, Gool EL, Nieuwland R, Recent developments in the nomenclature, presence, isolation, detection and clinical impact of extracellular vesicles. J THROMB HAEMOST 2016;14 (1):48-56 [PubMed]
van der Pol E, Mudde YD, Coumans FAW, van Leeuwen TG, Sturk A, Nieuwland R, Wound scabs protect regenerating tissue against harmful ultraviolet radiation. MED HYPOTHESES 2016;96:39-41 [PubMed]
van der Pol E. Detection of extracellular vesicles: size does matter. S.l.: s.n.; 2015. 222p. ISBN 9789462950696 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): van Leeuwen AGJM, Sturk A; copromotor(s): Nieuwland R)
Yuana Y, Böing AN, Grootemaat AE, van der Pol E, Hau CM, Cizmar P, Buhr E, Sturk A, Nieuwland R, Handling and storage of human body fluids for analysis of extracellular vesicles. J EXTRACELL VESICLES 2015;4:29260 [PubMed]
Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R, Single-step isolation of extracellular vesicles by size-exclusion chromatography. J EXTRACELL VESICLES 2014;3:23430 [PubMed]
Coumans FAW, van der Pol E, Böing AN, Hajji N, Sturk G, van Leeuwen TG, Nieuwland R, Reproducible extracellular vesicle size and concentration determination with tunable resistive pulse sensing. J EXTRACELL VESICLES 2014;3:25922 [PubMed]
van der Pol E, Coumans FAW, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, Sturk A, van Leeuwen TG, Nieuwland R, Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J THROMB HAEMOST 2014;12 (7):1182-1192 [PubMed]
van der Pol E, Coumans FAW, Sturk A, Nieuwland R, van Leeuwen TG, Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis. NANO LETT 2014;14 (11):6195-6201 [PubMed]
Varga Z, Yuana Y, Grootemaat AE, van der Pol E, Gollwitzer C, Krumrey M, Nieuwland R, Towards traceable size determination of extracellular vesicles. J EXTRACELL VESICLES 2014;3:23298 [PubMed]
Nguyen VD, Faber DJ, van der Pol E, van Leeuwen TG, Kalkman J, Dependent and multiple scattering in transmission and backscattering optical coherence tomography. OPT EXPRESS 2013;21 (24):29145-29156 [PubMed]
van der Pol E, Coumans F, Varga Z, Krumrey M, Nieuwland R, Innovation in detection of microparticles and exosomes. J THROMB HAEMOST 2013;11 (Suppl. 1):36-45 [PubMed]
Coumans FAW, van der Pol E, Terstappen LWMM, Flat-top illumination profile in an epifluorescence microscope by dual microlens arrays. CYTOM PART A 2012;81A (4):324-331 [PubMed]
Nieuwland R, van der Pol E, Gardiner C, Sturk A. Platelet-Derived Microparticles in: Michelson AD, editor. Platelets. 3rd ed. San Diego: Academic Press; 2012., p. 453-467
van den Akker J, van Weert A, Afink G, Bakker ENTP, van der Pol E, Böing AN, Nieuwland R, VanBavel E, Transglutaminase 2 is secreted from smooth muscle cells by transamidation-dependent microparticle formation. AMINO ACIDS 2012;42 (2-3):961-973 [PubMed]
van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R, Classification, Functions, and Clinical Relevance of Extracellular Vesicles. PHARMACOL REV 2012;64 (3):676-705 [PubMed]
van der Pol E, van Gemert MJC, Sturk A, Nieuwland R, van Leeuwen TG, Single vs. swarm detection of microparticles and exosomes by flow cytometry. J THROMB HAEMOST 2012;10 (5):919-930 [PubMed]
van der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG, Nieuwland R, Optical and non-optical methods for detection and characterization of microparticles and exosomes. J THROMB HAEMOST 2010;8 (12):2596-2607 [PubMed]
Key publications

van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R, Classification, Functions, and Clinical Relevance of Extracellular Vesicles. PHARMACOL REV 2012;64 (3):676-705 [PubMed]
van der Pol E, Hoekstra AG, Sturk A, Otto C, van Leeuwen TG, Nieuwland R, Optical and non-optical methods for detection and characterization of microparticles and exosomes. J THROMB HAEMOST 2010;8 (12):2596-2607 [PubMed]
van der Pol E, van Gemert MJC, Sturk A, Nieuwland R, van Leeuwen TG, Single vs. swarm detection of microparticles and exosomes by flow cytometry. J THROMB HAEMOST 2012;10 (5):919-930 [PubMed]
van der Pol E, Coumans FAW, Sturk A, Nieuwland R, van Leeuwen TG, Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis. NANO LETT 2014;14 (11):6195-6201 [PubMed]


All Publications

Curriculum Vitae

Please see: nl.linkedin.com/in/edwinvanderpol

Research programmes

Prof. dr. A.G.J.M. van Leeuwen MSc PhD (Biomedical photonics for functional and molecular imaging)

Other research related activities
  • Board of directors / Trustees / Supervisory board, Exometry B.V.
Current research funding
  • NWO Vrijgesteld