• Principal Investigator
  • Female

Dr. K.C. Wolthers MD PhD

Position: Associate professor (UHD)
Main activities: Research, Patient care
Specialisation: Medical Microbiology/virology
foto K.C. Wolthers is one of the AMC Principal Investigators
Focus of research:

Molecular epidemiology and pathogenesis of human parechoviruses and enteroviruses

AMC themes: Infection & Immunity
Departments: Medical Microbiology
Bruning AHL. Catching the Common Cold - Rapid detection and epidemiology of respiratory viruses. S.l.: s.n.; 2017. -p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Kuijpers TW, de Jong MD; copromotor(s): Wolthers KC, Pajkrt D)
Bruning AHL, de Kruijf WB, van Weert HCPM, Willems WLM, de Jong MD, Pajkrt D, Wolthers KC, Diagnostic performance and clinical feasibility of a point-of-care test for respiratory viral infections in primary health care. FAM PRACT 2017;34 (5):558-563 [PubMed]
Bruning AHL, Leeflang MMG, Vos JMBW, Spijker R, de Jong MD, Wolthers KC, Pajkrt D, Rapid Tests for Influenza, Respiratory Syncytial Virus, and Other Respiratory Viruses: A Systematic Review and Meta-analysis. CLIN INFECT DIS 2017;65 (6):1026-1032 [PubMed]
Bruning AHL, Leeflang MMG, Wolthers KC, Pajkrt D, Reply to Vos et al. CLIN INFECT DIS 2017;ahead of print [PubMed]
de Jong EP, van den Beuken MGA, van Elzakker EPM, Wolthers KC, Sprij AJ, Lopriore E, Walther FJ, Brus F, Epidemiology of Sepsis-like Illness in Young Infants: Major Role of Enterovirus and Human Parechovirus. PEDIATR INFECT DIS J 2017;ahead of print [PubMed]
de Kort H, Heutinck KM, Ruben JM, Ede V Silva A, Wolthers KC, Hamann J, ten Berge IJM, Primary Human Renal-Derived Tubular Epithelial Cells Fail to Recognize and Suppress BK Virus Infection. TRANSPLANTATION 2017;101 (8):1820-1829 [PubMed]
Karelehto E, van der Sanden S, Geraets JA, Domanska A, van der Linden L, Hoogendoorn D, Koen G, van Eijk H, Shakeel S, Beaumont T, de Jong M, Pajkrt D, Butcher SJ, Wolthers KC, Strain-dependent neutralization reveals antigenic variation of human parechovirus 3. SCI REP-UK 2017;7 (1):12075 [PubMed]
Benschop KSM, Geeraedts F, Beuvink B, Spit SA, Fanoy EB, Claas ECJ, Pas SD, Schuurman R, Verweij JJ, Bruisten SM, Wolthers KC, Niesters HGM, Koopmans M, Duizer E, Increase in ECHOvirus 6 infections associated with neurological symptoms in the Netherlands, June to August 2016. EUROSURVEILLANCE 2016;21 (39):30351-- [PubMed]
Benschop KSM, Rahamat-Langendoen JC, van der Avoort HGAM, Claas ECJ, Pas SD, Schuurman R, Verweij JJ, Wolthers KC, Niesters HGM, Koopmans MPG, VIRO-TypeNed, systematic molecular surveillance of enteroviruses in the Netherlands between 2010 and 2014. EUROSURVEILLANCE 2016;21 (39):30352-- [PubMed]
Ludlow M, Kortekaas J, Herden C, Hoffmann B, Tappe D, Trebst C, Griffin DE, Brindle HE, Solomon T, Brown AS, van Riel D, Wolthers KC, Pajkrt D, Wohlsein P, Martina BEE, Baumgärtner W, Verjans GM, Osterhaus ADME, Neurotropic virus infections as the cause of immediate and delayed neuropathology. ACTA NEUROPATHOL 2016;131 (2):159-184 [PubMed]
Shakeel S, Westerhuis BM, Domanska A, Koning RI, Matadeen R, Koster AJ, Bakker AQ, Beaumont T, Wolthers KC, Butcher SJ, Multiple capsid-stabilizing interactions revealed in a high-resolution structure of an emerging picornavirus causing neonatal sepsis. NAT COMMUN 2016;7:11387 [PubMed]
van der Linden L, Bruning AHL, Thomas XV, Minnaar RP, Rebers SPH, Schinkel J, de Jong MD, Pajkrt D, Wolthers KC, A molecular epidemiological perspective of rhinovirus types circulating in Amsterdam from 2007 to 2012. CLIN MICROBIOL INFEC 2016;22 (12):1002.e9-1002.e14 [PubMed]
van der Sanden SMG, Koen G, van Eijk H, Koekkoek SM, de Jong MD, Wolthers KC, Prediction of Protection against Asian Enterovirus 71 Outbreak Strains by Cross-neutralizing Capacity of Serum from Dutch Donors, the Netherlands. EMERG INFECT DIS 2016;22 (9):1562-1569 [PubMed]
Wildenbeest JG, Benschop KSM, Bouma-de Jongh S, Wolthers KC, Pajkrt D, Prolonged Shedding of Human Parechovirus in Feces of Young Children after Symptomatic Infection. PEDIATR INFECT DIS J 2016;35 (5):580-583 [PubMed]
Wildenbeest JG, van der Schee MP, Hashimoto S , Benschop KSM, Minnaar RP, Sprikkelman AB, Haarman EG, van Aalderen WMC, Sterk PJ, Pajkrt D, Wolthers KC, Prevalence of rhinoviruses in young children of an unselected birth cohort from the Netherlands. CLIN MICROBIOL INFEC 2016;22 (8):736.e9-736.e15 [PubMed]
Benschop KSM, Wildenbeest JG, Koen G, Minnaar RP, van Hemert FJ, Westerhuis BM, Pajkrt D, van den Broek PJ, Vossen ACTM, Wolthers KC, Genetic and antigenic structural characterization for resistance of echovirus 11 to pleconaril in an immunocompromised patient. J GEN VIROL 2015;96 (Part 3):571-579 [PubMed]
Bruning AHL, Thomas XV, van der Linden L, Wildenbeest JG, Minnaar RP, Jansen RR, de Jong MD, Sterk PJ, van der Schee MP, Wolthers KC, Pajkrt D, Clinical, virological and epidemiological characteristics of rhinovirus infections in early childhood: A comparison between non-hospitalised and hospitalised children. J CLIN VIROL 2015;73:120-126 [PubMed]
Bruning AHL, van der Sanden SMG, ten Hoedt AE, Wolthers KC, van Kaam AH, Pajkrt D, An atypical course of coxsackievirus A6 associated hand, foot and mouth disease in extremely low birth weight preterm twins. J CLIN VIROL 2015;65:20-22 [PubMed]
Hofstra JJ, Matamoros S, van de Pol MA, de Wever B, Tanck MW, Wendt-Knol H, Deijs M, van der Hoek L, Wolthers KC, Molenkamp R, Visser CE, Sterk PJ, Lutter R, de Jong MD, Changes in microbiota during experimental human Rhinovirus infection. BMC INFECT DIS 2015;15:336 [PubMed]
Shakeel S, Westerhuis BM, Ora A, Koen G, Bakker AQ, Claassen Y, Wagner K, Beaumont T, Wolthers KC, Butcher SJ, Structural Basis of Human Parechovirus Neutralization by Human Monoclonal Antibodies. J VIROL 2015;89 (18):9571-9580 [PubMed]
van der Linden L, Wolthers KC, van Kuppeveld FJM, Replication and Inhibitors of Enteroviruses and Parechoviruses. VIRUSES-BASEL 2015;7 (8):4529-4562 [PubMed]
Westerhuis BM, Benschop KSM, Koen G, Claassen YB, Wagner K, Bakker AQ, Wolthers KC, Beaumont T, Human Memory B Cells Producing Potent Cross-Neutralizing Antibodies against Human Parechovirus: Implications for Prevalence, Treatment, and Diagnosis. J VIROL 2015;89 (15):7457-7464 [PubMed]
Benschop KSM, Wolthers KC, The need for concerted action against the cousins of poliovirus. FUTURE VIROL 2014;9 (6):541-543
Bruning AHL, van Dijk K, van Eijk HWM, Koen G, van Woensel JBM, Kruisinga FH, Pajkrt D, Wolthers KC, Evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the Netherlands. DIAGN MICR INFEC DIS 2014;80 (4):292-293 [PubMed]
Janes VA, Minnaar R, Koen G, van Eijk H, Dijkman-de Haan K, Pajkrt D, Wolthers KC, Benschop KS, Presence of human non-polio enterovirus and parechovirus genotypes in an Amsterdam hospital in 2007 to 2011 compared to national and international published surveillance data: a comprehensive review. EUROSURVEILLANCE 2014;19 (46):15-23 [PubMed]
Majoor CJ , van de Pol MA, Kamphuisen PW, Meijers JCM, Molenkamp R, Wolthers KC, van der Poll T, Nieuwland R, Johnston SL, Sterk PJ, Bel EHD, Lutter R, van der Sluijs KF, Evaluation of coagulation activation after rhinovirus infection in patients with asthma and healthy control subjects: an observational study. RESP RES 2014;15 (1):14 [PubMed]
Murk JL, de Vries AC, GeurtsvanKessel CH, Aron G, Osterhaus AD, Wolthers KC, Fraaij PL, Persistent spiking fever in a child with acute myeloid leukemia and disseminated infection with enterovirus. J CLIN VIROL 2014;61 (3):453-455 [PubMed]
Westerhuis BM. The 3-dimensional play of human parechovirus infection: Cell, virus and antibody. S.l.: s.n.; 2014. 177p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): de Jong MD; copromotor(s): Wolthers KC)
Wildenbeest JG. Human enteroviruses and parechoviruses: Disease spectrum and need for treatment in young children. S.l.: s.n.; 2014. 237p. ISBN 9789461088567 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): de Jong MD, Kuijpers TW; copromotor(s): Pajkrt D, Wolthers KC)
Wildenbeest JG, Benschop KSM, Minnaar RP, Bouma-de Jongh S, Wolthers KC, Pajkrt D, Clinical relevance of positive human parechovirus type 1 and 3 PCR in stool samples. CLIN MICROBIOL INFEC 2014;20 (10):O640-O647 [PubMed]
Wolthers KC, de Jong MD, Is enterovirus 71-vaccinatie ook nodig in Nederland?. NED TIJDSCHR GENEESKD 2014;158:A7674 [PubMed]
Baan E, van der Sluis RM, Bakker ME, Bekker V, Pajkrt D, Jurriaans S, Kuijpers TW, Berkhout B, Wolthers KC, Paxton WA, Pollakis G, Human immunodeficiency virus type 1 gp120 envelope characteristics associated with disease progression differ in family members infected with genetically similar viruses. J GEN VIROL 2013;94 (1):20-29 [PubMed]
Brouwer MC, Jim KK, Benschop KS, Wolthers KC, van der Ende A, de Jong MD, van de Beek D, No evidence of viral coinfection in cerebrospinal fluid from patients with community-acquired bacterial meningitis. J INFECT DIS 2013;208 (1):182-184 [PubMed]
Koopmans M, Niesters HG, Benschop K, Wolthers K, Schuurman R, Pas SD, Claas EC, Kroneman A, Rahamat-Langendoen JC, Vennema H, van der Avoort H, Authors' reply: Application of Bayesian methods to the inference of phylogeny for enterovirus surveillance. EUROSURVEILLANCE 2013;18 (9):21 20410-22
Leendertse M, van Vugt M, Benschop KS, van Dijk K, Minnaar RP, van Eijk HWM, Hodiamont CJ, Wolthers KC, Pleurodynia caused by an echovirus 1 brought back from the tropics. J CLIN VIROL 2013;58 (2):490-493 [PubMed]
Niesters HG, Rossen JW, van der Avoort H, Baas D, Benschop K, Claas EC, Kroneman A, van Maarseveen N, Pas S, van Pelt W, Rahamat-Langendoen JC, Schuurman R, Vennema H, Verhoef L, Wolthers K, Koopmans M, Laboratory-based surveillance in the molecular era: the TYPENED model, a joint data-sharing platform for clinical and public health laboratories. EUROSURVEILLANCE 2013;18 (4):20387-56 [PubMed]
van der Sluijs KF, van de Pol MA, Kulik W, Dijkhuis A, Smids BS, van Eijk HW, Karlas JA, Molenkamp R, Wolthers KC, Johnston SL, van der Zee JS, Sterk PJ, Lutter R [Contributors: Berger M, Fens N, Yick D, Wagener AH , Amelink M, Reesink HJ, Bresser P, Kunst PWA , Venekamp LN, Majoor CJ , van Steenwijk RP, Jonkers RE]] , Systemic tryptophan and kynurenine catabolite levels relate to severity of rhinovirus-induced asthma exacerbation: a prospective study with a parallel-group design. THORAX 2013;68 (12):1122-1130 [PubMed]
Westerhuis B, Kolehmainen P, Benschop K, Nurminen N, Koen G, Koskiniemi M, Simell O, Knip M, Hyöty H, Wolthers K, Tauriainen S, Human parechovirus seroprevalence in Finland and the Netherlands. J CLIN VIROL 2013;58 (1):211-215 [PubMed]
Westerhuis BM, Jonker SCM, Mattao S, Benschop KSM, Wolthers KC, Growth characteristics of human parechovirus 1 to 6 on different cell lines and cross- neutralization of human parechovirus antibodies: a comparison of the cytopathic effect and real time PCR. VIROL J 2013;10:146 [PubMed]
Wildenbeest JG, Wolthers KC, Straver B, Pajkrt D, Successful IVIG Treatment of Human Parechovirus-Associated Dilated Cardiomyopathy in an Infant. PEDIATRICS 2013;132 (1):E243-E247 [PubMed]
Benschop K, Wildenbeest J, Pajkrt D, Wolthers K. Human Parechoviruses, New Players in the Pathogenesis of Viral Meningitis in: Wireko-Brobby G, editor. Meningitis. S.l.: InTech; 2012., p. 145-162
de Vries JJC, van der Eijk AA, Wolthers KC, Rusman LG, Pas SD, Molenkamp R, Claas EC, Kroes ACM, Vossen ACTM, Real-time PCR versus viral culture on urine as a gold standard in the diagnosis of congenital cytomegalovirus infection. J CLIN VIROL 2012;53 (2):167-170 [PubMed]
McLeish NJ, Witteveldt J, Clasper L, McIntyre C, McWilliam Leitch EC, Hardie A, Bennett S, Gunson R, Carman WF, Feeney SA, Coyle PV, Vipond B, Muir P, Benschop K, Wolthers K, Waris M, Osterback R, Johannessen I, Templeton K, Harvala H, Simmonds P, Development and Assay of RNA Transcripts of Enterovirus Species A to D, Rhinovirus Species A to C, and Human Parechovirus: Assessment of Assay Sensitivity and Specificity of Real-Time Screening and Typing Methods. J CLIN MICROBIOL 2012;50 (9):2910-2917 [PubMed]
Minnaar RP, Koen G, de Haan K, Wolthers KC, Benschop KSM, Evaluation of two (semi-)nested VP1 based-PCRs for typing enteroviruses directly from cerebral spinal fluid samples. J VIROL METHODS 2012;185 (2):228-233 [PubMed]
van den Berg-van de Glind GJ, de Vries JJC, Wolthers KC, Wiggers-de Bruine FT, Peeters-Scholte CMPCD, van den Hende M, van Wezel-Meijler G, A fatal course of neonatal meningo-encephalitis. J CLIN VIROL 2012;55 (2):91-94 [PubMed]
Westerhuis BM, Koen G, Wildenbeest JG, Pajkrt D, de Jong MD, Benschop KSM, Wolthers KC, Specific cell tropism and neutralization of human parechovirus types 1 and 3: implications for pathogenesis and therapy development. J GEN VIROL 2012;93 (11):2363-2370 [PubMed]
Wildenbeest JG, van den Broek PJ, Benschop KSM, Koen G, Wierenga PC, Vossen ACTM, Kuijpers TW, Wolthers KC, Pleconaril revisited: clinical course of chronic enteroviral meningoencephalitis after treatment correlates with in vitro susceptibility. ANTIVIR THER 2012;17 (3):459-466 [PubMed]
Jurriaans S, Back NKT, Wolthers KC, Ten years of HIV testing with fourth generation assays: the Amsterdam experience. J CLIN VIROL 2011;52 (Suppl. 1):S67-S69 [PubMed]
Menting S, Thai KTD, Nga TTT, Phuong HL, Klatser P, Wolthers KC, Binh TQ, de Vries PJ, Beld M, Internally controlled, generic real-time PCR for quantification and multiplex real-time PCR with serotype-specific probes for serotyping of dengue virus infections. ADV VIROL 2011;2011:514681 [PubMed]
Benschop K, Minnaar R, Koen G, van Eijk H, Dijkman K, Westerhuis B, Molenkamp R, Wolthers K, Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping. DIAGN MICR INFEC DIS 2010;68 (2):166-173 [PubMed]
Benschop KSM, de Vries M, Minnaar RP, Stanway G, van der Hoek L, Wolthers KC, Simmonds P, Comprehensive full-length sequence analyses of human parechoviruses: diversity and recombination. J GEN VIROL 2010;91 (Part 1):145-154 [PubMed]
Calvert J, Chieochansin T, Benschop KS, McWilliam Leitch EC, Drexler JF, Grywna K, da Costa Ribeiro H Jr, Drosten C, Harvala H, Poovorawan Y, Wolthers KC, Simmonds P, Recombination dynamics of human parechoviruses: investigation of type-specific differences in frequency and epidemiological correlates. J GEN VIROL 2010;91 (Part 5):1229-1238 [PubMed]
Harvala H, Wolthers KC, Simmonds P, Parechoviruses in children: understanding a new infection. CURR OPIN INFECT DIS 2010;23 (3):224-230 [PubMed]
van Houdt R, Bruisten SM, Geskus RB, Bakker M, Wolthers KC, Prins M, Coutinho RA, Ongoing transmission of a single hepatitis B virus strain among men having sex with men in Amsterdam. J VIRAL HEPATITIS 2010;17 (2):108-114 [PubMed]
Wildenbeest JG, Harvala H, Pajkrt D, Wolthers KC, The need for treatment against human parechoviruses: how, why and when?. EXPERT REV ANTI-INFE 2010;8 (12):1417-1429 [PubMed]
Benschop KSM. Clinical and molecular insights into human parechovirus infection. s.l.: s.n.; 2009. 217p. ISBN 9789088911125 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): de Jong MD, Vandenbroucke-Grauls CMJE; copromotor(s): Wolthers KC)
Pajkrt D, Benschop KSM, Westerhuis B, Molenkamp R, Spanjerberg L, Wolthers KC, Clinical Characteristics of Human Parechoviruses 4-6 Infections in Young Children. PEDIATR INFECT DIS J 2009;28 (11):1008-1010 [PubMed]
van Houdt R, van den Berg CHSB, Stolte IG, Bruisten SM, Dukers NHTM, Bakker M, Wolthers KC, Prins M, Coutinho RA, Two Decades of Hepatitis B Infections Among Drug Users in Amsterdam: Are They Still a High-Risk Group?. J MED VIROL 2009;81 (7):1163-1169 [PubMed]
Benschop K, Molenkamp R, van der Ham A, Wolthers K, Beld M, Rapid detection of human parechoviruses in clinical samples by real-time PCR. J CLIN VIROL 2008;41 (2):69-74 [PubMed]
Benschop KSM, Williams CH, Wolthers KC, Stanway G, Simmonds P, Widespread recombination within human parechoviruses: analysis of temporal dynamics and constraints. J GEN VIROL 2008;89 (Part 4):1030-1035 [PubMed]
Benschop KSM, Wolthers KC, Humane Parechovirussen, niet te missen. NED TIJDSCHR MED MICROBIOL 2008;16 (4):9-12
Benschop K, Stanway G, Wolthers KC. New Human Parechoviruses: six and counting in: Scheld WM, Hammer SM, Hughes JM, editors. Emerging Infections 8. S.l.: ASM; 2008. 4, p. 53-74
Benschop K, Thomas X, Serpenti C, Molenkamp R, Wolthers K, High prevalence of Human Parechovirus (HPeV) genotypes in the Amsterdam region and the identification of specific HPeV variants by direct genotyping of stool samples. J CLIN MICROBIOL 2008;46 (12):3965-3970 [PubMed]
Harvala H, Robertson I, Leitch ECM, Benschop K, Wolthers KC, Templeton K, Simmonds P, Epidemiology and clinical associations of human parechovirus respiratory infections. J CLIN MICROBIOL 2008;46 (10):3446-3453 [PubMed]
van Binnendijk RS, Hahné S, Timen A, van Kempen G, Kohl RHG, Boot HJ, Wolthers KC, Wetsteijn JCFM, de Vries A, Westert K, Brown KE, de Swart RL, Air travel as a risk factor for introduction of measles in a highly vaccinated population. VACCINE 2008;26 (46):5775-5777 [PubMed]
Wetsteyn JCFM, de Rond WMJ, Schreuder MCJ, de Boer HEL, van Binnendijk RS, Wolthers KC, Een uitbraak van mazelen op de Spoedeisende Hulp. NED TIJDSCHR GENEESKD 2008;152 (37):2032-2036 [PubMed]
Wolthers KC, Benschop KSM, Schinkel J, Molenkamp R, Bergevoet RM, Spijkerman IJB, Kraakman HC, Pajkrt D, Human parechoviruses as an important viral cause of sepsislike illness and meningitis in young children. CLIN INFECT DIS 2008;47 (3):358-363 [PubMed]
van de Laar TJW, van der Bij AK, Prins M, Bruisten SM, Brinkman K, Ruys TA, van der Meer JTM, de Vries HJC, Mulder JW, van Agtmael M, Jurriaans S, Wolthers KC, Coutinho RA, Increase in HCV incidence among men who have sex with men in Amsterdam most likely caused by sexual transmission. J INFECT DIS 2007;196 (2):230-238 [PubMed]
van den Berg CHSB, Smit C, Bakker M, Geskus RB, Berkhout B, Jurriaans S, Coutinho RA, Wolthers KC, Prins M, Major decline of hepatitis C virus incidence rate over two decades in a cohort of drug users. EUR J EPIDEMIOL 2007;22 (3):183-193 [PubMed]
Wolthers KC, Jurriaans S, Valkuilen in de diagnostiek van het humaan immunodeficiëntievirus (HIV). NED TIJDSCHR MED MICROBIOL 2007;15:7-11
Bekker V, Scherpbier HJ, Steingrover R, Jurriaans S, Lange JMA, Wolthers KC, Kuijpers TW, Viral dynamics after starting first-line HAART in HIV-1-infected children. AIDS 2006;20 (4):517-523 [PubMed]
Benschop KSM, Schinkel J, Luken ME, van den Broek PJM, Beersma MFC, Menelik N, van Eijk HWM, Zaaijer HL, Vandenbroucke-Grauls CMJE, Beld MGHM, Wolthers KC, Fourth human parechovirus serotype. EMERG INFECT DIS 2006;12 (10):1572-1575 [PubMed]
Benschop KSM, Schinkel J, Minnaar RP, Pajkrt D, Spanjerberg L, Kraakman HC, Berkhout B, Zaaijer HL, Beld MGHM, Wolthers KC, Human parechovirus infections in Dutch children and the association between serotype and disease severity. CLIN INFECT DIS 2006;42 (2):204-210 [PubMed]
Prins JM, Wolthers KC, Human metapneumovirus: a new pathogen in children and adults. NETH J MED 2004;62 (6):177-179 [PubMed]
van der Hoek L, Pyrc K, Jebbink MF, Vermeulen-Oost W, Berkhout RJM, Wolthers KC, Wertheim-van Dillen PME, Kaandorp J, Spaargaren J, Berkhout B, Identification of a new human coronavirus. NAT MED 2004;10 (4):368-373 [PubMed]
Verhave M, Krasinski SD, Christian SI, van Schaik S, van den Brink GR, Doting EMH, Maas SM, Wolthers KC, Grand RJ, Montgomery RK, Regulatory regions in the rat lactase-phlorizin hydrolase gene that control cell-specific expression. J PEDIATR GASTR NUTR 2004;39 (3):275-285 [PubMed]
Fraaij PL, van Rossum AM, Wolthers KC, Vossen AC, Jeucken YM, Kuijpers JH, van Lochem EG, Roos MT, de Groot R, Hartwig NG, Initation of highly active antiretroviral therapy leads to an HIV-specific immune response in a seronegative infant. AIDS 2003;17 (1):138-140 [PubMed]
Wolthers KC, Kornelisse RF, Platenkamp GJJM, Schuurman-van der Lem MI, van der Schee C, Hartwig NG, Verduin CM, A case of Mycoplasma hominis meningo-encephalitis in a full-term infant: rapid recovery after start of treatment with ciprofloxacin. EUR J PEDIATR 2003;162 (7-8):514-516 [PubMed]
van Baarle D, Wolthers KC, Hovenkamp E, Niesters HGM, Osterhaus ADME, Miedema F, van Oers MHJ, Absolute level of Epstein-Barr virus DNA in human immunodeficiency virus type 1 infection is not predictive of AIDS-related non-Hodgkin lymphoma. J INFECT DIS 2002;186 (3):405-409 [PubMed]
van Baarle D, Hovenkamp E, Callan MF, Wolthers KC, Kostense S, Tan LC, Niesters HG, Osterhaus AD, McMichael AJ, van Oers MH, Miedema F, Dysfunctional Epstein-Barr virus (EBV)-specific CD8(+) T lymphocytes and increased EBV load in HIV-1 infected individuals progressing to AIDS-related non-Hodgkin lymphoma. BLOOD 2001;98 (1):146-155 [PubMed]
van Esser JW, Niesters HG, Thijsen SF, Meijer E, Osterhaus AD, Wolthers KC, Boucher CA, Gratama JW, Budel LM, van der Holt B, van Loon AM, Löwenberg B, Verdonck LF, Cornelissen JJ, Molecular quantification of viral load in plasma allows for fast and accurate prediction of response to therapy of Epstein-Barr virus-associated lymphoproliferative disease after allogeneic stem cell transplantation. BRIT J HAEMATOL 2001;113 (3):814-821 [PubMed]
van Rossum AM, Scherpbier HJ, van Lochem EG, Pakker NG, Slieker WA, Wolthers KC, Roos MT, Kuijpers JH, Hooijkaas H, Hartwig NG, Geelen SP, Wolfs TF, Lange JM, Miedema F, de Groot R, Therapeutic immune reconstitution in HIV-1-infected children is independent of their age and pretreatment immune status. AIDS 2001;15 (17):2267-2275 [PubMed]
Wolthers KC, de Roda Husman AM. The Amsterdam Cohort Studies on HIV infection and AIDS: a summary of the results 1996-2000. Amsterdam: s.n.; 2001. 74p. ISBN 90-9015506-6
Clark DR, Wolthers KC. T cell dynamics and renewal in HIV-1 infection in: Schuitemaker H, Miedema F, editors. AIDS pathogenesis. Amsterdam: Kluwer; 2000., p. 55-64
Niesters HG, van Esser J, Fries E, Wolthers KC, Cornelissen J, Osterhaus AD, Development of a real-time quantitative assay for detection of Epstein-Barr virus. J CLIN MICROBIOL 2000;38 (2):712-715 [PubMed]
Clark DR, de Boer RJ, Wolthers KC, Miedema F, T cell dynamics in HIV-1 infection. ADV IMMUNOL 1999;73:301-327 [PubMed]
Hamann D, Kostense S, Wolthers KC, Otto SA, Baars PA, Miedema F, van Lier RA, Evidence that human CD8+CD45RA+CD27- cells are induced by antigen and evolve through extensive rounds of division. INT IMMUNOL 1999;11 (7):1027-1033 [PubMed]
Wolthers KC, Noest AJ, Otto SA, Miedema F, de Boer RJ, Normal telomere lengths in naive and memory CD4+ T cells in HIV type 1 infection: a mathematical interpretation. AIDS RES HUM RETROV 1999;15 (12):1053-1062 [PubMed]
Wolthers KC, Otto SA, Wisman GB, Fleury S, Reiss P, ten Kate RW, van der Zee AG, Miedema F, Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. BLOOD 1999;93 (3):1011-1019 [PubMed]
Fleury S, de Boer RJ, Rizzardi GP, Wolthers KC, Otto SA, Welbon CC, Graziosi C, Knabenhans C, Soudeyns H, Bart PA, Gallant S, Corpataux JM, Gillet M, Meylan P, Schnyder P, Meuwly JY, Spreen W, Glauser MP, Miedema F, Pantaleo G, Limited CD4+ T-cell renewal in early HIV-1 infection: effect of highly active antiretroviral therapy. NAT MED 1998;4 (7):794-801 [PubMed]
Wolthers KC, Miedema F, Telomeres and HIV-1 infection: in search of exhaustion. TRENDS MICROBIOL 1998;6 (4):144-147 [PubMed]
Wolthers KC, Schuitemaker H, Miedema F, Rapid CD4+ T-cell turnover in HIV-1 infection: a paradigm revisited. IMMUNOL TODAY 1998;19 (1):44-48 [PubMed]
Wolthers KC, Otto SA, Lens SM, van Lier RA, Miedema F, Meyaard L, Functional B cell abnormalities in HIV type 1 infection: role of CD40L and CD70. AIDS RES HUM RETROV 1997;13 (12):1023-1029 [PubMed]
Wolthers KC, Bea G, Wisman A, Otto SA, de Roda Husman AM, Schaft N, de Wolf F, Goudsmit J, Coutinho RA, van der Zee AG, Meyaard L, Miedema F, T cell telomere length in HIV-1 infection: no evidence for increased CD4+ T cell turnover. SCIENCE 1996;274 (5292):1543-1547 [PubMed]
Wolthers KC, Otto SA, Lens SM, Kolbach DN, van Lier RA, Miedema F, Meyaard L, Increased expression of CD80, CD86 and CD70 on T cells from HIV-infected individuals upon activation in vitro: regulation by CD4+ T cells. EUR J IMMUNOL 1996;26 (8):1700-1706 [PubMed]
Meyaard L, Kuiper H, Otto SA, Wolthers KC, van Lier RA, Miedema F, Evidence for intact costimulation via CD28 and CD27 molecules in hyporesponsive T cells from human immunodeficiency virus-infected individuals. EUR J IMMUNOL 1995;25 (1):232-237 [PubMed]
Key publications

Shakeel S, Westerhuis BM, Domanska A, Koning RI, Matadeen R, Koster AJ, Bakker AQ, Beaumont T, Wolthers KC, Butcher SJ, Multiple capsid-stabilizing interactions revealed in a high-resolution structure of an emerging picornavirus causing neonatal sepsis. NAT COMMUN 2016;7:11387 [PubMed]
van der Sanden SMG, Koen G, van Eijk H, Koekkoek SM, de Jong MD, Wolthers KC, Prediction of Protection against Asian Enterovirus 71 Outbreak Strains by Cross-neutralizing Capacity of Serum from Dutch Donors, the Netherlands. EMERG INFECT DIS 2016;22 (9):1562-1569 [PubMed]
van der Linden L, Bruning AHL, Thomas XV, Minnaar RP, Rebers SPH, Schinkel J, de Jong MD, Pajkrt D, Wolthers KC, A molecular epidemiological perspective of rhinovirus types circulating in Amsterdam from 2007 to 2012. CLIN MICROBIOL INFEC 2016;22 (12):1002.e9-1002.e14 [PubMed]
Wildenbeest JG, van der Schee MP, Hashimoto S , Benschop KSM, Minnaar RP, Sprikkelman AB, Haarman EG, van Aalderen WMC, Sterk PJ, Pajkrt D, Wolthers KC, Prevalence of rhinoviruses in young children of an unselected birth cohort from the Netherlands. CLIN MICROBIOL INFEC 2016;22 (8):736.e9-736.e15 [PubMed]
Wildenbeest JG, Benschop KSM, Minnaar RP, Bouma-de Jongh S, Wolthers KC, Pajkrt D, Clinical relevance of positive human parechovirus type 1 and 3 PCR in stool samples. CLIN MICROBIOL INFEC 2014;20 (10):O640-O647 [PubMed]


All Publications

Curriculum Vitae

1984-1993   Medical School University of Groningen (1984-1989) and University of Amsterdam (1989-1993)

1992-1993   Glaxo Fellowship, duration 1 year, New England Medical Center, Boston
                       “Transcriptional Regulation of the Rat Lactase Gene” Project leaders: Prof. Dr. H.A. Büller/ Prof. Dr. R.A Grand
 

1993              Medical Degree at the University of Amsterdam1993-1998    PhD studies at the Central Laboratory of the Blood Transfusion Service (Sanquin). Thesis on        T cell function and T cell dynamics in HIV-1 infection. 
                       Promotor Prof. F. Miedema

1998-2003   Medical Microbiology/Virology training at the Erasmus University Rotterdam, Laboratory of Virology, Head Prof A. Osterhaus

2003-present    Staff member Clinical Virologist at Dept Medical Microbiology, AMC

2014-present    Coordinator FP7 Marie Curie IAPP consortium "AIROPIco"

Other activities

• Review Editor for the Journal of Clinical Virology 2008- present
• Board member Antibiotics Committee AMC, Academic Medical Center, Amsterdam, 2010 – 2014
• Board member Dutch Society for Clinical Virology, 2010 – 2014
• Board member National Quality Control Committee for Medical Microbiological Laboratories (SKML) division Cell Culture, 2010- present
• Review Editor for the Editorial Board of Frontiers in Virology 2010- present
*Member Subcommittee Medical and Veterinary Aspects of COGEM (Commissie genetische Modificatie) 2013- 2016
 

Research programmes

Molecular epidemiology and pathogenesis of human picornaviruses

In 2006, I started a new research line on human picornaviruses within the Dept of Medical Microbiology. We established the role of human parechoviruses (HPeVs) as serious and frequently occurring pathogens in young infants, and two new types HPeV4 and HPeV14 were discovered. We defined classification rules for HPeVs in good agreement with the Picornavirus Studygroup; nowadays, 16 types are known. We were the first to show significant differences in clinical symptoms and age of infection between HPeV3 and HPeV1, and the importance of HPeV3 in viral CNS infections.

Our laboratory is specialized in fast and reliable laboratory diagnosis for entero- rhino- and parechovirus infections, all members of the Picornavirus family. We use virus genotyping to show the widespreadness of the different virus genoypes and their relation with clinical symptoms. We have expertise in molecular evolution studies.  

Our research within this area is translational. The role of maternal antibody protection in picornavirus infection in neonates is studied, as well as differences in cell tropism between picornavirus types in relation to disease severity. Furthermore we focus on treatment possibilities and resistance mechanisms.

In 2013, I became coordinator of an EU FP7 IAPP grant called AIROPico, bringing together researchers from the picornavirus field, evolutionary biologists, structural biologists, and scientists as well as SMEs developing antiviral compounds and new diagnostic methods. The coming four years we will collaborate on increasing knowledge on picornavirus pathogenesis, development of fast diagnostic techniques and development of antiviral therapy for this large virus group.  

 

Faculty
Dr. K.C. Wolthers MD PhD
D. Pajkrt MD PhD

Postdocs
K.S.M. Benschop PhD
Dr. L. van der Linden PhD
Dr. S.M.G. van der Sanden PhD
X. Thomas PhD
Drs. B.M. Westerhuis

PhD Students
L. Brouwer
S.E. Karelehto

Others
D. de Blauw BSc
L. Bosch BSc
H.W.M. van Eijk
G. Koen
Dr. E.A. Koppel PhD
R. Minnaar BSc
R. Venkatachalam PhD

D. Pajkrt MD PhD (Impact of pediatric viral infections, focus on hiv and picornavirus infection)

Other research related activities
  • None reported
Current research funding
  • Europese Unie
  • ZonMw