• Male

Dr. ir. F.A.W. Coumans

Position: Postdoc
Main activities: Research
Specialisation: Flow cytometry, Detection of sub micron particles, cancer
foto
Focus of research:

Background Microparticles and exosomes are extracellular vesicles (EV) that are present in all bodily fluids. EV are part of the intercellular communication system. Changes in EV concentration and composition are associated with e.g. coagulation disorders and metastatic carcinomas. Consequently, concentration, size distribution and origin of these EV are currently explored for medical applications such as diagnosis, prognosis, and monitoring of treatment.

Problem Typically, the concentration is 10^10 EV per mL and their diameter range is 30-1000 nm. Because current instruments are optimised for analysing cells, and the median diameter (~60 nm) of EV is ~100-fold smaller than cells, EV detection is a major challenge.

Research Vesicle research is a young field. The Aim of my research is to develop, impelement and understand techniques that can determine the concentration of a single vesicle type in complex samples (e.g. plasma). These techniques include flow cytometry, surface plasmon resonance imaging, electron microscopy, resistive pulse sensing and nanoparticle tracking.

My focus is on flow cytometry. The flow cytometer is capable of measuring concentration, size and multiple fluorescence signals on thousands of individual EV/s. However, (1) size determination by flow cytometry is inaccurate and imprecise, (2) the presence of impurities like protein aggregates reduces specificity of measurements, and (3) sensitivity to detect EV < 250 nm is lacking. Through smart innovations on commercial flow cytometers, we can improve these systems for researchers worldwide.

AMC themes: Cardiovascular Diseases, Oncology
Departments: Biomedical Engineering and Physics
Coumans FAW, Brisson AR, Buzas EI, Dignat-George F, Drees EEE, El-Andaloussi S, Emanueli C, Gasecka A, Hendrix A, Hill AF, Lacroix R, Lee Y, van Leeuwen TG, Mackman N, Mäger I, Nolan JP, van der Pol E, Pegtel DM, Sahoo S, Siljander PRM, Sturk G, de Wever O, Nieuwland R, Methodological Guidelines to Study Extracellular Vesicles. CIRC RES 2017;120 (10):1632-1648 [PubMed]
Coumans FAW, Gool EL, Nieuwland R, Bulk immunoassays for analysis of extracellular vesicles. PLATELETS 2017;28 (3):242-248 [PubMed]
Gool EL, Stojanovic I, Schasfoort RBM, Sturk A, van Leeuwen TG, Nieuwland R, Terstappen LWMM, Coumans FAW, Surface Plasmon Resonance is an Analytically Sensitive Method for Antigen Profiling of Extracellular Vesicles. CLIN CHEM 2017;63 (10):1633-1641 [PubMed]
van der Pol E, Mudde YD, Coumans FAW, van Leeuwen TG, Sturk A, Nieuwland R, Wound scabs protect regenerating tissue against harmful ultraviolet radiation. MED HYPOTHESES 2016;96:39-41 [PubMed]
Vogel R, Coumans FAW, Maltesen RG, Böing AN, Bonnington KE, Broekman ML, Broom MF, Buzás EI, Christiansen G, Hajji N, Kristensen SR, Kuehn MJ, Lund SM, Maas SLN, Nieuwland R, Osteikoetxea X, Schnoor R, Scicluna BJ, Shambrook M, de Vrij J, Mann SI, Hill AF, Pedersen S, A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing. J EXTRACELL VESICLES 2016;5:31242 [PubMed]
Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R, Single-step isolation of extracellular vesicles by size-exclusion chromatography. J EXTRACELL VESICLES 2014;3:23430 [PubMed]
Coumans FAW, van der Pol E, Böing AN, Hajji N, Sturk G, van Leeuwen TG, Nieuwland R, Reproducible extracellular vesicle size and concentration determination with tunable resistive pulse sensing. J EXTRACELL VESICLES 2014;3:25922 [PubMed]
van der Pol E, Coumans FAW, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, Sturk A, van Leeuwen TG, Nieuwland R, Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J THROMB HAEMOST 2014;12 (7):1182-1192 [PubMed]
van der Pol E, Coumans FAW, Sturk A, Nieuwland R, van Leeuwen TG, Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis. NANO LETT 2014;14 (11):6195-6201 [PubMed]
Coumans FAW, Siesling S, Terstappen LWMM, Detection of cancer before distant metastasis. BMC CANCER 2013;13:283 [PubMed]
Coumans FAW, van Dalum G, Beck M, Terstappen LWMM, Filter Characteristics Influencing Circulating Tumor Cell Enrichment from Whole Blood. PLOS ONE 2013;8 (4):e61770 [PubMed]
Coumans FAW, van Dalum G, Beck M, Terstappen LWMM, Filtration Parameters Influencing Circulating Tumor Cell Enrichment from Whole Blood. PLOS ONE 2013;8 (4):e61774 [PubMed]
Ligthart ST, Coumans FAW, Bidard FC, Simkens LHJ, Punt CJA, de Groot MR, Attard G, de Bono JS, Pierga JY, Terstappen LWMM, Circulating Tumor Cells Count and Morphological Features in Breast, Colorectal and Prostate Cancer. PLOS ONE 2013;8 (6):e67148 [PubMed]
van der Pol E, Coumans F, Varga Z, Krumrey M, Nieuwland R, Innovation in detection of microparticles and exosomes. J THROMB HAEMOST 2013;11 (Suppl. 1):36-45 [PubMed]
Coumans FAW, Ligthart ST, Uhr JW, Terstappen LWMM, Challenges in the Enumeration and Phenotyping of CTC. CLIN CANCER RES 2012;18 (20):5711-5718 [PubMed]
Coumans FAW, van der Pol E, Terstappen LWMM, Flat-top illumination profile in an epifluorescence microscope by dual microlens arrays. CYTOM PART A 2012;81A (4):324-331 [PubMed]
Swennenhuis JF, Foulk B, Coumans FAW, Terstappen LWMM, Construction of repeat-free fluorescence in situ hybridization probes. NUCLEIC ACIDS RES 2012;40 (3):e20 [PubMed]
Ligthart ST, Coumans FAW, Attard G, Cassidy AM, de Bono JS, Terstappen LWMM, Unbiased and Automated Identification of a Circulating Tumour Cell Definition That Associates with Overall Survival. PLOS ONE 2011;6 (11):e27419 [PubMed]
Rao C, Bui T, Connelly M, Doyle G, Karydis I, Middleton MR, Clack G, Malone M, Coumans FAW, Terstappen LWMM, Circulating melanoma cells and survival in metastatic melanoma. INT J ONCOL 2011;38 (3):755-760 [PubMed]
Coumans FAW, Doggen CJM, Attard G, de Bono JS, Terstappen LWMM, All circulating EpCAM+CK+CD45-objects predict overall survival in castration-resistant prostate cancer. ANN ONCOL 2010;21 (9):1851-1857 [PubMed]
Key publications

Coumans FAW, Doggen CJM, Attard G, de Bono JS, Terstappen LWMM, All circulating EpCAM+CK+CD45-objects predict overall survival in castration-resistant prostate cancer. ANN ONCOL 2010;21 (9):1851-1857 [PubMed]
Coumans FAW, Ligthart ST, Uhr JW, Terstappen LWMM, Challenges in the Enumeration and Phenotyping of CTC. CLIN CANCER RES 2012;18 (20):5711-5718 [PubMed]
Ligthart ST, Coumans FAW, Attard G, Cassidy AM, de Bono JS, Terstappen LWMM, Unbiased and Automated Identification of a Circulating Tumour Cell Definition That Associates with Overall Survival. PLOS ONE 2011;6 (11):e27419 [PubMed]
Coumans FAW, Siesling S, Terstappen LWMM, Detection of cancer before distant metastasis. BMC CANCER 2013;13:283 [PubMed]
Böing AN, van der Pol E, Grootemaat AE, Coumans FAW, Sturk A, Nieuwland R, Single-step isolation of extracellular vesicles by size-exclusion chromatography. J EXTRACELL VESICLES 2014;3:23430 [PubMed]


All Publications

Research programmes

Prof. dr. A.G.J.M. van Leeuwen MSc PhD (Biomedical photonics for functional and molecular imaging)

Dr. R. Nieuwland (Extracellular vesicles and platelets in health and disease)

Other research related activities
  • None reported
Current research funding
  • None reported