• Male

G.B. Afink PhD

Position: Scientific staff member (UD)
Main activities: Research
Specialisation: Molecular Biology
foto
Focus of research:

About 25% of all pregnancies are complicated by gestational disease, of which severe hypertension (i.e. early preeclampsia and HELLP syndrome) and preterm labour are the leading cause of pregnancy-related maternal morbidity and mortality and poor neonatal outcome. Although there is consensus that the causal organ for these gestational diseases is the placenta the underlying multifactorial molecular causes and early and reliable clinical biomarkers that explain and predict development of obstetrical disease during the course of pregnancy are not available.

AMC themes: Other research
Departments: Center for Reproductive Medicine, Obstetrics and Gynaecology
Ho L, van Dijk M, Chye STJ, Messerschmidt DM, Chng SC, Ong S, Yi LK, Boussata S, Goh GHY, Afink GB, Lim CY, Dunn NR, Solter D, Knowles BB, Reversade B, ELABELA deficiency promotes preeclampsia and cardiovascular malformations in mice. SCIENCE 2017;357 (6352):707-+ [PubMed]
Prins JR, van der Hoorn MLP, Keijser R, Ris-Stalpers C, van Beelen E, Afink GB, Claas FHJ, van der Post JAM, Scherjon SA, Higher decidual EBI3 and HLA-G mRNA expression in preeclampsia: Cause or consequence of preeclampsia. HUM IMMUNOL 2016;77 (1):68-70 [PubMed]
Amraoui F, Hassani Lahsinoui H, Boussata S, Keijser R, Veenboer GJM, Middeldorp S, van der Post JAM, Ris-Stalpers C, Afink GB, van den Born BJH, Placental expression of heparan sulfate 3-O-sulfotransferase-3A1 in normotensive and pre-eclamptic pregnancies. PLACENTA 2015;36 (11):1218-1224 [PubMed]
Fernando F, Keijser R, Henneman P, van der Kevie-Kersemaekers AMF, Mannens MM, van der Post JA, Afink GB, Ris-Stalpers C, The idiopathic preterm delivery methylation profile in umbilical cord blood DNA. BMC GENOMICS 2015;16 (1):736 [PubMed]
Jebbink JM, Boot RG, Keijser R, Moerland PD, Aten J, Veenboer GJM, van Wely M, Buimer M, Ver Loren van Themaat E, Aerts JMFG, van der Post JAM, Afink GB, Ris-Stalpers C, Increased glucocerebrosidase expression and activity in preeclamptic placenta. PLACENTA 2015;36 (2):160-169 [PubMed]
Jebbink J, Veenboer G, Boussata S, Keijser R, Kremer AE, Elferink RO, van der Post J, Afink G, Ris-Stalpers C, Total bile acids in the maternal and fetal compartment in relation to placental ABCG2 expression in preeclamptic pregnancies complicated by HELLP syndrome. BBA-MOL BASIS DIS 2015;1852 (1):131-136 [PubMed]
van Uitert M, Moerland PD, Enquobahrie DA, Laivuori H, van der Post JAM, Ris-Stalpers C, Afink GB, Meta-Analysis of Placental Transcriptome Data Identifies a Novel Molecular Pathway Related to Preeclampsia. PLOS ONE 2015;10 (7):e0132468 [PubMed]
Vissenberg R, Manders VD, Mastenbroek S, Fliers E, Afink GB, Ris-Stalpers C, Goddijn M, Bisschop PH, Pathophysiological aspects of thyroid hormone disorders/thyroid peroxidase autoantibodies and reproduction. HUM REPROD UPDATE 2015;21 (3):378-387 [PubMed]
Amraoui F, Spijkers L, Hassani Lahsinoui H, Vogt L, van der Post J, Peters S, Afink G, Ris-Stalpers C, van den Born BJ, SFlt-1 elevates blood pressure by augmenting endothelin-1-mediated vasoconstriction in mice. PLOS ONE 2014;9 (3):e91897 [PubMed]
Jebbink JM. Preeclamptic disorders of pregnancy; Novel molecular insights. S.l.: s.n.; 2014. 159p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): van der Post JAM; copromotor(s): Afink GB, Ris-Stalpers C)
Jebbink J, Tabbers M, Afink G, Beuers U, Oude Elferink R, Ris-Stalpers C, van der Post J, HELLP syndrome preceded by intrahepatic cholestasis of pregnancy: one serious itch. BMJ CASE REP 2014;2014:bcr2013203208 [PubMed]
Karamat FA, Oudman I, Ris-Stalpers C, Afink GB, Keijser R, Clark JF, van Montfrans GA, Brewster LM, Resistance artery creatine kinase mRNA and blood pressure in humans. HYPERTENSION 2014;63 (1):68-73 [PubMed]
Böing AN, Stap J, Hau CM, Afink GB, Ris-Stalpers C, Reits EA, Sturk A, van Noorden CJF, Nieuwland R, Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles. BBA-MOL CELL RES 2013;1833 (8):1844-1852 [PubMed]
de Laat MWM, Pieper PG, Oudijk MA, Mulder BJM, Christoffels VM, Afink GB, Postma AV, Ris-Stalpers C, The clinical and molecular relations between idiopathic preterm labor and maternal congenital heart defects. REPROD SCI 2013;20 (2):190-201 [PubMed]
Kleinrouweler CE, van Uitert M, Moerland PD, Ris-Stalpers C, van der Post JAM, Afink GB, Differentially expressed genes in the pre-eclamptic placenta: a systematic review and meta-analysis. PLOS ONE 2013;8 (7):e68991 [PubMed]
Burniat A, Pirson I, Vilain C, Kulik W, Afink G, Moreno-Reyes R, Corvilain B, Abramowicz M, Iodotyrosine Deiodinase Defect Identified via Genome-Wide Approach. J CLIN ENDOCR METAB 2012;97 (7):E1276-E1283 [PubMed]
Jebbink J, Wolters A, Fernando F, Afink G, van der Post J, Ris-Stalpers C, Molecular genetics of preeclampsia and HELLP syndrome - A review. BBA-MOL BASIS DIS 2012;1822 (12):1960-1969 [PubMed]
Liu Z, Afink GB, ten Dijke P, Soluble fms-like tyrosine kinase 1 and soluble endoglin are elevated circulating anti-angiogenic factors in pre-eclampsia. PREGNANCY HYPERTENS 2012;2 (4):358-367
van den Akker J, van Weert A, Afink G, Bakker ENTP, van der Pol E, Böing AN, Nieuwland R, VanBavel E, Transglutaminase 2 is secreted from smooth muscle cells by transamidation-dependent microparticle formation. AMINO ACIDS 2012;42 (2-3):961-973 [PubMed]
Jebbink J, Keijser R, Veenboer G, van der Post J, Ris-Stalpers C, Afink G, Expression of Placental FLT1 Transcript Variants Relates to Both Gestational Hypertensive Disease and Fetal Growth. HYPERTENSION 2011;58 (1):70-U129 [PubMed]
Nazarenko I. Modeling PDGF-driven gliomagenesis in the mouse. s.l.: s.n.; 2011. -p. ISBN 9789174574517 Proefschrift Karolinska Institute, Stockholm, Sweden, Oncology-Pathology. (Promotor(s): Nistér M; copromotor(s): Lindström MS, Afink GB)
Nazarenko I, Hedrén A, Sjödin H, Orrego A, Andrae J, Afink GB, Nistér M, Lindström MS, Brain abnormalities and glioma-like lesions in mice overexpressing the long isoform of PDGF-A in astrocytic cells. PLOS ONE 2011;6 (4):e18303 [PubMed]
Afink GB, Veenboer G, de Randamie J, Keijser R, Meischl C, Niessen H, Ris-Stalpers C, Initial Characterization of C16orf89, A Novel Thyroid-Specific Gene. THYROID 2010;20 (7):811-821 [PubMed]
Hede SM, Hansson I, Afink GB, Eriksson A, Nazarenko I, Andrae J, Genove G, Westermark B, Nistér M, GFAP promoter driven transgenic expression of PDGFB in the mouse brain leads to glioblastoma in a Trp53 null background. GLIA 2009;57 (11):1143-1153 [PubMed]
Afink G, Kulik W, Overmars H, de Randamie J, Veenboer T, van Cruchten A, Craen M, Ris-Stalpers C, Molecular characterization of iodotyrosine dehalogenase deficiency in patients with hypothyroidism. J CLIN ENDOCR METAB 2008;93 (12):4894-4901 [PubMed]
Toepoel M, Joosten PHLJ, Knobbe CB, Afink GB, Zotz RB, Steegers-Theunissen RPM, Reifenberger G, van Zoelen EJJ, Haplotype-specific expression of the human PDGFRA gene correlates with the risk of glioblastomas. INT J CANCER 2008;123 (2):322-329 [PubMed]
de Bustos C, Smits A, Strömberg B, Collins VP, Nistér M, Afink G, A PDGFRA promoter polymorphism, which disrupts the binding of ZNF148, is associated with primitive neuroectodermal tumours and ependymomas. J MED GENET 2005;42 (1):31-37 [PubMed]
Zhang XQ, Afink GB, Hu XR, Forsberg-Nilsson K, Nistér M, Gli1 is not required for Pdgfralpha expression during mouse embryonic development. DIFFERENTIATION 2005;73 (2-3):109-119 [PubMed]
Afink G, Westermark UK, Lammerts E, Nistér M, C/EBP is an essential component of PDGFRA transcription in MG-63 cells. BIOCHEM BIOPH RES CO 2004;315 (2):313-318 [PubMed]
Andrae J, Afink G, Zhang XQ, Wurst W, Nistér M, Forced expression of platelet-derived growth factor B in the mouse cerebellar primordium changes cell migration during midline fusion and causes cerebellar ectopia. MOL CELL NEUROSCI 2004;26 (2):308-321 [PubMed]
Joosten PHLJ, Toepoel M, van Oosterhout D, Afink GB, van Zoelen EJJ, A regulating element essential for PDGFRA transcription is recognized by neural tube defect-associated PRX homeobox transcription factors. BBA-MOL BASIS DIS 2002;1588 (3):254-260 [PubMed]
Andrae J, Hansson I, Afink GB, Nistér M, Platelet-derived growth factor receptor-alpha in ventricular zone cells and in developing neurons. MOL CELL NEUROSCI 2001;17 (6):1001-1013 [PubMed]
Hu X, Pang T, Guo Z, Pontén J, Nistér M, Afink GB, Oncogene lineages of human papillomavirus type 16 E6, E7 and E5 in preinvasive and invasive cervical squamous cell carcinoma. J PATHOL 2001;195 (3):307-311 [PubMed]
Ling G, Ahmadian A, Persson A, Undén AB, Afink G, Williams C, Uhlén M, Toftgård R, Lundeberg J, Pontén F, PATCHED and p53 gene alterations in sporadic and hereditary basal cell cancer. ONCOGENE 2001;20 (53):7770-7778 [PubMed]
Guo Z, Hu X, Afink G, Pontén F, Wilander E, Pontén J, Comparison of chromosome 3p deletions between cervical precancers synchronous with and without invasive cancer. INT J CANCER 2000;86 (4):518-523 [PubMed]
Hu X, Guo Z, Pang T, Li Q, Afink G, Pontén J, Immunohistochemical and DNA sequencing analysis on human mismatch repair gene MLH1 in cervical squamous cell carcinoma with LOH of this gene. ANTICANCER RES 2000;20 (1A):171-175 [PubMed]
Sun T, Jayatilake D, Afink GB, Ataliotis P, Nistér M, Richardson WD, Smith HK, A human YAC transgene rescues craniofacial and neural tube development in PDGFRalpha knockout mice and uncovers a role for PDGFRalpha in prenatal lung growth. DEVELOPMENT 2000;127 (21):4519-4529 [PubMed]
Joosten PH, Hol FA, van Beersum SE, Peters H, Hamel BC, Afink GB, van Zoelen EJ, Mariman EC, Altered regulation of platelet-derived growth factor receptor-alpha gene-transcription in vitro by spina bifida-associated mutant Pax1 proteins. P NATL ACAD SCI USA 1998;95 (24):14459-14463 [PubMed]
Zhang XQ, Afink GB, Svensson K, Jacobs JJ, Günther T, Forsberg-Nilsson K, van Zoelen EJ, Westermark B, Nistér M, Specific expression in mouse mesoderm- and neural crest-derived tissues of a human PDGFRA promoter/lacZ transgene. MECH DEVELOP 1998;70 (1-2):167-180 [PubMed]
Afink GB, Nistér M, Stassen BH, Joosten PH, Rademakers PJ, Bongcam-Rudloff E, van Zoelen EJ, Mosselman S, Molecular cloning and functional characterization of the human platelet-derived growth factor alpha receptor gene promoter. ONCOGENE 1995;10 (8):1667-1672 [PubMed]
Afink GB, van Alewijk DC, de Roos AD, van Zoelen EJ, Lysophosphatidic acid and bradykinin have opposite effects on phenotypic transformation of normal rat kidney cells. J CELL BIOCHEM 1994;56 (4):480-489 [PubMed]
Lahaye DH, Afink GB, Bleijs DA, van Alewijk DC, van Zoelen EJ, Effect of bradykinin on loss of density-dependent growth inhibition of normal rat kidney cells. CELL MOL BIOL 1994;40 (5):717-721 [PubMed]
van Zoelen EJ, Peters PH, Afink GB, van Genesen S, de Roos DG, van Rotterdam W, Theuvenet AP, Bradykinin-induced growth inhibition of normal rat kidney (NRK) cells is paralleled by a decrease in epidermal-growth-factor receptor expression. BIOCHEM J 1994;298 ( Pt 2):335-340 [PubMed]
Singh AK, Afink GB, Venglarik CJ, Wang RP, Bridges RJ, Colonic Cl channel blockade by three classes of compounds. AM J PHYSIOL 1991;261 (1 Part 1):C51-C63 [PubMed]
Key publications

Kleinrouweler CE, van Uitert M, Moerland PD, Ris-Stalpers C, van der Post JAM, Afink GB, Differentially expressed genes in the pre-eclamptic placenta: a systematic review and meta-analysis. PLOS ONE 2013;8 (7):e68991 [PubMed]
Jebbink J, Keijser R, Veenboer G, van der Post J, Ris-Stalpers C, Afink G, Expression of Placental FLT1 Transcript Variants Relates to Both Gestational Hypertensive Disease and Fetal Growth. HYPERTENSION 2011;58 (1):70-U129 [PubMed]
Afink G, Kulik W, Overmars H, de Randamie J, Veenboer T, van Cruchten A, Craen M, Ris-Stalpers C, Molecular characterization of iodotyrosine dehalogenase deficiency in patients with hypothyroidism. J CLIN ENDOCR METAB 2008;93 (12):4894-4901 [PubMed]
de Bustos C, Smits A, Strömberg B, Collins VP, Nistér M, Afink G, A PDGFRA promoter polymorphism, which disrupts the binding of ZNF148, is associated with primitive neuroectodermal tumours and ependymomas. J MED GENET 2005;42 (1):31-37 [PubMed]
van Uitert M, Moerland PD, Enquobahrie DA, Laivuori H, van der Post JAM, Ris-Stalpers C, Afink GB, Meta-Analysis of Placental Transcriptome Data Identifies a Novel Molecular Pathway Related to Preeclampsia. PLOS ONE 2015;10 (7):e0132468 [PubMed]


All Publications

Research programmes

Prof. dr. J.A.M. van der Post MD PhD (Reproductive Health, placental disease and maternal and fetal diseases)

Prof. dr. S. Repping (Reproduction & Development -- Human Reproduction)

Other research related activities
  • None reported
Current research funding
  • AMC