• Principal Investigator
  • Male

Dr. R.H. Houtkooper

Position: Scientific staff member (UD)
Main activities: Research
Specialisation: Mitochondrial metabolism
foto R.H. Houtkooper is one of the AMC Principal Investigators
Focus of research:
  • Mitochondria in health and disease
  • Metabolic aging
AMC themes: Metabolic disorders and gastrointestinal diseases
Departments: Laboratory Genetic Metabolic Diseases, Paediatrics: Metabolic Diseases
Chatzispyrou IA, Alders M, Guerrero-Castillo S, Zapata Perez R, Haagmans MA, Mouchiroud L, Koster J, Ofman R, Baas F, Waterham HR, Spelbrink JN, Auwerx J, Mannens MM, Houtkooper RH, Plomp AS, A homozygous missense mutation in ERAL1, encoding a mitochondrial rRNA chaperone, causes Perrault syndrome. HUM MOL GENET 2017;ahead of print [PubMed]
Gao AW, Chatzispyrou IA, Kamble R, Liu YJ, Herzog K, Smith RL, van Lenthe H, Vervaart MAT, van Cruchten A, Luyf AC, van Kampen A, Pras-Raves ML, Vaz FM, Houtkooper RH, A sensitive mass spectrometry platform identifies metabolic changes of life history traits in C-elegans. SCI REP-UK 2017;7 (1):2408 [PubMed]
Lachmandas E, Boutens L, Ratter JM, Hijmans A, Hooiveld GJ, Joosten LAB, Rodenburg RJ, Fransen JAM, Houtkooper RH, van Crevel R, Netea MG, Stienstra R, Microbial stimulation of different Toll-like receptor signalling pathways induces diverse metabolic programmes in human monocytes. NAT MICROBIOL 2017;2 (3):16246 [PubMed]
Lankelma JM, Birnie E, Weehuizen TAF, Scicluna BP, Belzer C, Houtkooper RH, Roelofs JJTH, de Vos AF, van der Poll T, Budding AE, Wiersinga WJ, The gut microbiota as a modulator of innate immunity during melioidosis. PLOS NEGLECT TROP D 2017;11 (4):e0005548 [PubMed]
Palir N, Ruiter JPN, Wanders RJA, Houtkooper RH, Identification of enzymes involved in oxidation of phenylbutyrate. J LIPID RES 2017;58 (5):955-961 [PubMed]
Paolini NA, Attwood M, Sondalle SB, Vieira CMDS, van Adrichem AM, di Summa FM, O'Donohue MF, Gleizes PE, Rachuri S, Briggs JW, Fischer R, Ratcliffe PJ, Wlodarski MW, Houtkooper RH, von Lindern M, Kuijpers TW, Dinman JD, Baserga SJ, Cockman ME, MacInnes AW, A Ribosomopathy Reveals Decoding Defective Ribosomes Driving Human Dysmorphism. AM J HUM GENET 2017;100 (3):506-522 [PubMed]
Wüst RCI, Visser G, Wanders RJA, Houtkooper RH, Ketones and inborn errors of metabolism: old friends revisited. J INHERIT METAB DIS 2017;40 (1):3-4 [PubMed]
Bleeker JC, Houtkooper RH, Sirtuin activation as a therapeutic approach against inborn errors of metabolism. J INHERIT METAB DIS 2016;39 (4):565-572 [PubMed]
Cantó C, Houtkooper RH. Sirtuins and Aging in: Houtkooper R, editor. Sirtuins. Amsterdam: Springer; 2016., p. 213-227
Hornberger TA, Carter HN, Hood DA, Figueiredo VC, Dupont-Versteegden EE, Peterson CA, McCarthy JJ, Camera DM, Hawley JA, Chaillou T, Cheng AJ, Nader GA, Wüst RCI, Houtkooper RH, Commentaries on Viewpoint: The rigorous study of exercise adaptations: Why mRNA might not be enough. J APPL PHYSIOL 2016;121 (2):597-600 [PubMed]
Houtkooper R, editor, Sirtuins. Amsterdam: Springer; 2016. 288p. ISBN 978-94-024-0961-1 (print); 978-94-024-0962-8 (electronic) (Proteins and Cell Regulation; 10)
Houtkooper RH. NAD+ as a Pharmacological Tool to Boost Sirtuin Activity in: Houtkooper R, editor. Sirtuins. Amsterdam: Springer; 2016., p. 29-40
Koopman M, Michels H, Dancy BM, Kamble R, Mouchiroud L, Auwerx J, Nollen EAA, Houtkooper RH, A screening-based platform for the assessment of cellular respiration in Caenorhabditis elegans. NAT PROTOC 2016;11 (10):1798-1816 [PubMed]
Kulkarni SS, Joffraud M, Boutant M, Ratajczak J, Gao AW, Maclachlan C, Hernandez-Alvarez MI, Raymond F, Metairon S, Descombes P, Houtkooper RH, Zorzano A, Cantó C, Mfn1 Deficiency in the Liver Protects Against Diet-Induced Insulin Resistance and Enhances the Hypoglycemic Effect of Metformin. DIABETES 2016;65 (12):3552-3560 [PubMed]
Schooneman MG, Houtkooper RH, Hollak CEM, Wanders RJA, Vaz FM, Soeters MR, Houten SM, The impact of altered carnitine availability on acylcarnitine metabolism, energy expenditure and glucose tolerance in diet-induced obese mice. BBA-MOL BASIS DIS 2016;1862 (8):1375-1382 [PubMed]
Thiels C, Fleger M, Huemer M, Rodenburg RJ, Vaz FM, Houtkooper RH, Haack TB, Prokisch H, Feichtinger RG, Lücke T, Mayr JA, Wortmann SB, Atypical Clinical Presentations of TAZ Mutations: An Underdiagnosed Cause of Growth Retardation?. JIMD REPORTS 2016;29:89-93 [PubMed]
Wust RCI, Houtkooper RH, EXERCISE ADAPTATIONS: THE WHOLE IS GREATER THAN THE SUM OF ITS PARTS. J APPL PHYSIOL 2016;121 (2):599-600
Chatzispyrou IA, Held NM, Mouchiroud L, Auwerx J, Houtkooper RH, Tetracycline antibiotics impair mitochondrial function and its experimental use confounds research. CANCER RES 2015;75 (21):4446-4449 [PubMed]
Diekman EF, Ferdinandusse S, van der Pol L, Waterham HR, Ruiter JPN, Ijlst L, Wanders RJ, Houten SM, Wijburg FA, Blank AC, Asselbergs FW, Houtkooper RH, Visser G, Fatty acid oxidation flux predicts the clinical severity of VLCAD deficiency. GENET MED 2015;17 (12):989-994 [PubMed]
Doddaballapur A, Michalik KM, Manavski Y, Lucas T, Houtkooper RH, You X, Chen W, Zeiher AM, Potente M, Dimmeler S, Boon RA, Laminar shear stress inhibits endothelial cell metabolism via KLF2-mediated repression of PFKFB3. ARTERIOSCL THROM VAS 2015;35 (1):137-145 [PubMed]
Held NM, Houtkooper RH, Mitochondrial quality control pathways as determinants of metabolic health. BIOESSAYS 2015;37 (8):867-876 [PubMed]
Kooijman S, van den Berg R, Ramkisoensing A, Boon MR, Kuipers EN, Loef M, Zonneveld TCM, Lucassen EA, Sips HCM, Chatzispyrou IA, Houtkooper RH, Meijer JH, Coomans CP, Biermasz NR, Rensen PCN, Prolonged daily light exposure increases body fat mass through attenuation of brown adipose tissue activity. P NATL ACAD SCI USA 2015;112 (21):6748-6753 [PubMed]
Moullan N, Mouchiroud L, Wang X, Ryu D, Williams EG, Mottis A, Jovaisaite V, Frochaux MV, Quiros PM, Deplancke B, Houtkooper RH, Auwerx J, Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research. CELL REP 2015;10 (10):1681-1691 [PubMed]
Vatner DE, Yan L, Lai L, Yuan C, Mouchiroud L, Pachon RE, Zhang J, Dillinger JG, Houtkooper RH, Auwerx J, Vatner SF, Type 5 adenylyl cyclase disruption leads to enhanced exercise performance. AGING CELL 2015;14 (6):1075-1084 [PubMed]
Wang X, Ryu D, Houtkooper RH, Auwerx J, Antibiotic use and abuse: a threat to mitochondria and chloroplasts with impact on research, health, and environment. BIOESSAYS 2015;37 (10):1045-1053 [PubMed]
Andreux PA, Mouchiroud L, Wang X, Jovaisaite V, Mottis A, Bichet S, Moullan N, Houtkooper RH, Auwerx J, A method to identify and validate mitochondrial modulators using mammalian cells and the worm C. elegans. SCI REP-UK 2014;4:5285 [PubMed]
Chatzispyrou IA, Houtkooper RH, The two-faced progeria gene and its implications in aging and metabolism. EMBO REP 2014;15 (5):470-471 [PubMed]
Gao AW, Cantó C, Houtkooper RH, Mitochondrial response to nutrient availability and its role in metabolic disease. EMBO MOL MED 2014;6 (5):580-589 [PubMed]
Gao AW, Houtkooper RH, Mitochondrial fission: firing up mitochondria in brown adipose tissue. EMBO J 2014;33 (5):401-402 [PubMed]
Stienstra R, Dijk W, van Beek L, Jansen H, Heemskerk M, Houtkooper RH, Denis S, van Harmelen V, Willems van Dijk K, Tack CJ, Kersten S, Mannose-binding lectin is required for the effective clearance of apoptotic cells by adipose tissue macrophages during obesity. DIABETES 2014;63 (12):4143-4153 [PubMed]
Wijnen WJ, van der Made I, van den Oever S, Hiller M, de Boer BA, Picavet DI, Chatzispyrou IA, Houtkooper RH, Tijsen AJ, Hagoort J, van Veen H, Everts V, Ruijter JM, Pinto YM, Creemers EE, Cardiomyocyte-specific miRNA-30c over-expression causes dilated cardiomyopathy. PLOS ONE 2014;9 (5):e96290 [PubMed]
Ye C, Lou W, Li Y, Chatzispyrou IA, Hüttemann M, Lee I, Houtkooper RH, Vaz FM, Chen S, Greenberg ML, Deletion of the cardiolipin-specific phospholipase Cld1 rescues growth and life span defects in the tafazzin mutant: implications for Barth syndrome. J BIOL CHEM 2014;289 (6):3114-3125 [PubMed]
Andreux P. Identification of genetic determinants and pharmacological modulators of mitochondrial function. S.l.: s.n.; 2013. 194p. Proefschrift École polytechnique fédérale de Lausanne EPFL, Faculté des sciences de la vie. (Promotor(s): Auwerx J; copromotor(s): Houtkooper RHL)
Andreux PA, Houtkooper RH, Auwerx J, Pharmacological approaches to restore mitochondrial function. NAT REV DRUG DISCOV 2013;12 (6):465-483 [PubMed]
Gonzalvez F, D'Aurelio M, Boutant M, Moustapha A, Puech JP, Landes T, Arnauné-Pelloquin L, Vial G, Taleux N, Slomianny C, Wanders RJ, Houtkooper RH, Bellenguer P, Møller IM, Gottlieb E, Vaz FM, Manfredi G, Petit PX, Barth syndrome: cellular compensation of mitochondrial dysfunction and apoptosis inhibition due to changes in cardiolipin remodeling linked to tafazzin (TAZ) gene mutation. BBA-MOL BASIS DIS 2013;1832 (8):1194-1206 [PubMed]
Houtkooper RH, Mouchiroud L, Ryu D, Moullan N, Katsyuba E, Knott G, Williams RW, Auwerx J, Mitonuclear protein imbalance as a conserved longevity mechanism. NATURE 2013;497 (7450):451-+ [PubMed]
Mouchiroud L, Houtkooper RH, Auwerx J, NAD(+) metabolism: A therapeutic target for age-related metabolic disease. CRIT REV BIOCHEM MOL 2013;48 (4):397-408 [PubMed]
Mouchiroud L, Houtkooper RH, Moullan N, Katsyuba E, Ryu D, Cantó C, Mottis A, Jo YS, Viswanathan M, Schoonjans K, Guarente L, Auwerx J, The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling. CELL 2013;154 (2):430-441 [PubMed]
Andreux PA, Williams EG, Koutnikova H, Houtkooper RH, Champy MF, Henry H, Schoonjans K, Williams RW, Auwerx J, Systems genetics of metabolism: the use of the BXD murine reference panel for multiscalar integration of traits. CELL 2012;150 (6):1287-1299 [PubMed]
Cantó C, Houtkooper RH, Pirinen E, Youn DY, Oosterveer MH, Cen Y, Fernandez-Marcos PJ, Yamamoto H, Andreux PA, Cettour-Rose P, Gademann K, Rinsch C, Schoonjans K, Sauve AA, Auwerx J, The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. CELL METAB 2012;15 (6):838-847 [PubMed]
Hinard V, Mikhail C, Pradervand S, Curie T, Houtkooper RH, Auwerx J, Franken P, Tafti M, Key electrophysiological, molecular, and metabolic signatures of sleep and wakefulness revealed in primary cortical cultures. J NEUROSCI 2012;32 (36):12506-12517 [PubMed]
Houtkooper RH, Auwerx J, Exploring the therapeutic space around NAD(+). J CELL BIOL 2012;199 (2):205-209 [PubMed]
Houtkooper RH, Pirinen E, Auwerx J, Sirtuins as regulators of metabolism and healthspan. NAT REV MOL CELL BIO 2012;13 (4):225-238 [PubMed]
Janiszewska M, Suvà ML, Riggi N, Houtkooper RH, Auwerx J, Clément-Schatlo V, Radovanovic I, Rheinbay E, Provero P, Stamenkovic I, Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells. GENE DEV 2012;26 (17):1926-1944 [PubMed]
Kloek JJ, Maréchal X, Roelofsen J, Houtkooper RH, van Kuilenburg ABP, Kulik W, Bezemer R, Nevière R, van Gulik TM, Heger M, Cholestasis is associated with hepatic microvascular dysfunction and aberrant energy metabolism before and during ischemia-reperfusion. ANTIOXID REDOX SIGN 2012;17 (8):1109-1123 [PubMed]
Acehan D, Vaz F, Houtkooper RH, James J, Moore V, Tokunaga C, Kulik W, Wansapura J, Toth MJ, Strauss A, Khuchua Z, Cardiac and Skeletal Muscle Defects in a Mouse Model of Human Barth Syndrome. J BIOL CHEM 2011;286 (2):899-908 [PubMed]
Bai P, Cantó C, Oudart H, Brunyánszki A, Cen Y, Thomas C, Yamamoto H, Huber A, Kiss B, Houtkooper RH, Schoonjans K, Schreiber V, Sauve AA, Menissier-de Murcia J, Auwerx J, PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation. CELL METAB 2011;13 (4):461-468 [PubMed]
Houtkooper RH, Argmann C, Houten SM, Cantó C, Jeninga EH, Andreux PA, Thomas C, Doenlen R, Schoonjans K, Auwerx J, The metabolic footprint of aging in mice. SCI REP-UK 2011;1:134 [PubMed]
Schug ZT, Gonzalvez F, Houtkooper RH, Vaz FM, Gottlieb E, BID is cleaved by caspase-8 within a native complex on the mitochondrial membrane. CELL DEATH DIFFER 2011;18 (3):538-548 [PubMed]
Timmers S, Konings E, Bilet L, Houtkooper RH, van de Weijer T, Goossens GH, Hoeks J, van der Krieken S, Ryu D, Kersten S, Moonen-Kornips E, Hesselink MKC, Kunz I, Schrauwen-Hinderling VB, Blaak EE, Auwerx J, Schrauwen P, Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans. CELL METAB 2011;14 (5):612-622 [PubMed]
Houtkooper RH, Auwerx J, Obesity: New life for antidiabetic drugs. NATURE 2010;466 (7305):443-444 [PubMed]
Houtkooper RH, Cantó C, Wanders RJ, Auwerx J, The Secret Life of NAD(+): An Old Metabolite Controlling New Metabolic Signaling Pathways. ENDOCR REV 2010;31 (2):194-223 [PubMed]
Houtkooper RH, Williams RW, Auwerx J, Metabolic networks of longevity. CELL 2010;142 (1):9-14 [PubMed]
Xu FY, McBride H, Acehan D, Vaz FM, Houtkooper RH, Lee RM, Mowat MA, Hatch GM, The dynamics of cardiolipin synthesis post-mitochondrial fusion. BBA-BIOMEMBRANES 2010;1798 (8):1577-1585 [PubMed]
Zaltsman Y, Shachnai L, Yivgi-Ohana N, Schwarz M, Maryanovich M, Houtkooper RH, Vaz FM, de Leonardis F, Fiermonte G, Palmieri F, Gillissen B, Daniel PT, Jimenez E, Walsh S, Koehler CM, Roy SS, Walter L, Hajnóczky G, Gross A, MTCH2/MIMP is a major facilitator of tBID recruitment to mitochondria. NAT CELL BIOL 2010;12 (6):553-562 [PubMed]
Acehan D, Khuchua Z, Houtkooper RH, Malhotra A, Kaufman J, Vaz FM, Ren M, Rockman HA, Stokes DL, Schlame M, Distinct effects of tafazzin deletion in differentiated and undifferentiated mitochondria. MITOCHONDRION 2009;9 (2):86-95 [PubMed]
Houtkooper RHL. Cardiolipin metabolism in Barth syndrome. s.l.: s.n.; 2009. 122p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Wanders RJA; copromotor(s): Vaz FM)
Houtkooper RH, Lenthe Hv, Stet FS, Wanders RJA, Kulik W, Vaz FM, HPLC-massaspectrometrie voor de diagnose van het Barth-syndroom. ANALYSE (AMSTERDAM) 2009;64 (7):200-205
Houtkooper RH, Rodenburg RJ, Thiels C, van Lenthe H, Stet F, Poll-The BT, Stone JE, Steward CG, Wanders RJ, Smeitink J, Kulik W, Vaz FM, Cardiolipin and monolysocardiolipin analysis in fibroblasts, lymphocytes, and tissues using high-performance liquid chromatography-mass spectrometry as a diagnostic test for Barth syndrome. ANAL BIOCHEM 2009;387 (2):230-237 [PubMed]
Houtkooper RH, Turkenburg M, Poll-The BT, Karall D, Pérez-Cerdá C, Morrone A, Malvagia S, Wanders RJ, Kulik W, Vaz FM, The enigmatic role of tafazzin in cardiolipin metabolism. BBA-BIOMEMBRANES 2009;1788 (10):2003-2014 [PubMed]
Rijken PJ, Houtkooper RH, Akbari H, Brouwers JF, Koorengevel MC, de Kruijff B, Frentzen M, Vaz FM, de Kroon AIPM, Cardiolipin molecular species with shorter acyl chains accumulate in Saccharomyces cerevisiae mutants lacking the acyl coenzyme A-binding protein Acb1p: new insights into acyl chain remodeling of cardiolipin. J BIOL CHEM 2009;284 (40):27609-27619 [PubMed]
Gonzalvez F, Schug ZT, Houtkooper RH, Mackenzie ED, Brooks DG, Wanders RJA, Petit PX, Vaz FM, Gottlieb E, Cardiolipin provides an essential activating platform for caspase-8 on mitochondria. J CELL BIOL 2008;183 (4):681-696 [PubMed]
Houtkooper RH, Vaz FM, Cardiolipin, the heart of mitochondrial metabolism. CELL MOL LIFE SCI 2008;65 (16):2493-2506 [PubMed]
Kulik W, van Lenthe H, Stet FS, Houtkooper RH, Kemp H, Stone JE, Steward CG, Wanders RJ, Vaz FM, Bloodspot assay using HPLC-tandem mass spectrometry for detection of Barth syndrome. CLIN CHEM 2008;54 (2):371-378 [PubMed]
Zeharia A, Shaag A, Houtkooper RH, Hindi T, de Lonlay P, Erez G, Hubert L, Saada A, de Keyzer Y, Eshel G, Vaz FM, Pines O, Elpeleg O, Mutations in LPIN1 cause recurrent acute myoglobinuria in childhood. AM J HUM GENET 2008;83 (4):489-494 [PubMed]
Houtkooper RH, Akbari H, van Lenthe H, Kulik W, Wanders RJA, Frentzen M, Vaz FM, Identification and characterization of human cardiolipin synthase. FEBS LETT 2006;580 (13):3059-3064 [PubMed]
Vaz FM, Houtkooper RH, Valianpour F, Barth PG, Wanders RJA, Only one splice variant of the human TAZ gene encodes a functional protein with a role in cardiolipin metabolism. J BIOL CHEM 2003;278 (44):43089-43094 [PubMed]
Key publications

Houtkooper RH, Mouchiroud L, Ryu D, Moullan N, Katsyuba E, Knott G, Williams RW, Auwerx J, Mitonuclear protein imbalance as a conserved longevity mechanism. NATURE 2013;497 (7450):451-+ [PubMed]
Houtkooper RH, Pirinen E, Auwerx J, Sirtuins as regulators of metabolism and healthspan. NAT REV MOL CELL BIO 2012;13 (4):225-238 [PubMed]
Mouchiroud L, Houtkooper RH, Moullan N, Katsyuba E, Ryu D, Cantó C, Mottis A, Jo YS, Viswanathan M, Schoonjans K, Guarente L, Auwerx J, The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling. CELL 2013;154 (2):430-441 [PubMed]
Moullan N, Mouchiroud L, Wang X, Ryu D, Williams EG, Mottis A, Jovaisaite V, Frochaux MV, Quiros PM, Deplancke B, Houtkooper RH, Auwerx J, Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research. CELL REP 2015;10 (10):1681-1691 [PubMed]
Andreux PA, Houtkooper RH, Auwerx J, Pharmacological approaches to restore mitochondrial function. NAT REV DRUG DISCOV 2013;12 (6):465-483 [PubMed]


All Publications

Research programmes

Translational metabolism

Faculty
Dr. R.H. Houtkooper

Postdocs
Dr. A.W. MacInnes
Dr. R.L. Smith
Dr. M. Van Weeghel
Dr. R.C.I. Wüst

PhD Students
Drs. J.C. Bleeker MD
I.A. Chatzispyrou
E.G. Daniels
W. Gao
N.M. Held
S.J.G. Knottnerus
J. Liu
M. Molenaars

Others
Ing. A.M. Van Adrichem
Ing. S. Denis
Ir. L. IJlst
R.S. Kamble MSc
Dr. M. Lezzerini

Prof. dr. R.J.A. Wanders (Biochemistry & Enzymology of Metabolic Disorders)

Other research related activities
  • Membership of editorial board / Editorship, FRONTIERS IN PHYSIOLOGY
  • Membership of editorial board / Editorship, MOLECULAR METABOLISM
Current research funding
  • AMC
  • AMC (Vrijgesteld)
  • Amsterdam University College, UvA
  • Diamond Blackfan Anaemia (DBA) Charity
  • Europese Unie
  • ZonMw