• Principal Investigator
  • Male

Dr. S. Mastenbroek PhD

Position: Scientific staff member (UD)
Main activities: Patient care, Research
Specialisation: Clinical Embryologist
foto S. Mastenbroek is one of the AMC Principal Investigators
Focus of research:

Dr. Sebastiaan Mastenbroek is an Assistant Professor and one of the Clinical Embryologists of the Center for Reproductive Medicine of the Academic Medical Center.

An important focus of his research in past years has been Preimplantation Genetic Screening (PGS) and human preimplantation embryology. PGS is a technique to select embryos in an IVF treatment based on their chromosomal content. In 2007, publication of his randomized controlled trial on PGS in the New England Journal of Medicine started a fiercely debated controversy on the use of PGS as it showed that the technique lowered pregnancy rates after IVF instead of increasing the pregnancy rates. He then published research that provided technical as well as biological reasons for the inefficacy of PGS.

In a broader perspective he is interested in ovarian aging, early human development, implantation, assisted reproductive techniques and evidence based laboratory practice. He is supervising multiple randomized controlled trials in IVF, involved in the development of techniques that are able to analyse single oocytes and embryos, and uses these techniques to find biological plausibility for the clinical outcomes of his trials.

He is a board member of the SAF foundation (stichting-saf.nl), chair of the LSFD laboratory working group, board member of the special interest group ART of the Dutch Society of Obstetrics and Gynaecology, associate editor of Human Reproduction Online, editor of the Cochrane Gynaecology and Fertility Group, and member of the Science Committee of the Dutch Society for Clinical Embryology. 

 

ORCID: orcid.org/0000-0002-7550-2924

AMC themes: Reproduction and development
Departments: Center for Reproductive Medicine, Obstetrics and Gynaecology
Braakhekke M, Mol F, Mastenbroek S, Mol BWJ, van der Veen F, Equipoise and the RCT. HUM REPROD 2017;32 (2):257-260 [PubMed]
Kleijkers SHM, Mantikou E, Slappendel E, Consten D, van Echten-Arends J, Wetzels AM, van Wely M, Smits LJM, van Montfoort APA, Repping S, Dumoulin JCM, Mastenbroek S, Reply II: Embryo culture media effects. HUM REPROD 2017;32 (3):717-718 [PubMed]
Wong KM, van Wely M, Mol F, Repping S, Mastenbroek S, Fresh versus frozen embryo transfers in assisted reproduction. COCHRANE DB SYST REV 2017;3 (3):CD011184 [PubMed]
Zheng Y, Jongejan A, Mulder CL, Mastenbroek S, Repping S, Wang Y, Li J, Hamer G, Trivial role for NSMCE2 during in vitro proliferation and differentiation of male germline stem cells. REPRODUCTION 2017;154 (3):181-195 [PubMed]
Franz C, Böing AN, Montag M, Strowitzki T, Markert UR, Mastenbroek S, Nieuwland R, Toth B, Extracellular vesicles in human follicular fluid do not promote coagulation. REPROD BIOMED ONLINE 2016;33 (5):652-655 [PubMed]
Kleijkers SHM, Mantikou E, Slappendel E, Consten D, van Echten-Arends J, Wetzels AM, van Wely M, Smits LJM, van Montfoort APA, Repping S, Dumoulin JCM, Mastenbroek S, Influence of embryo culture medium (G5 and HTF) on pregnancy and perinatal outcome after IVF: a multicenter RCT. HUM REPROD 2016;31 (10):2219-2230 [PubMed]
Mantikou E, Bruning O, Mastenbroek S, Repping S, Breit TM, de Jong M, Evaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis. FERTIL STERIL 2016;105 (2):511-9.e4 [PubMed]
Mantikou E, Jonker MJ, Wong KM, van Montfoort APA, de Jong M, Breit TM, Repping S, Mastenbroek S, Factors affecting the gene expression of in vitro cultured human preimplantation embryos. HUM REPROD 2016;31 (2):298-311 [PubMed]
Sermon K, Capalbo A, Cohen J, Coonen E, de Rycke M, de Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, Grifo J, Handyside A, Harper J, Kokkali G, Mastenbroek S, Meldrum D, Meseguer M, Montag M, Munné S, Rienzi L, Rubio C, Scott K, Scott R, Simon C, Swain J, Treff N, Ubaldi F, Vassena R, Vermeesch JR, Verpoest W, Wells D, Geraedts J, The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. MOL HUM REPROD 2016;22 (8):545-557 [PubMed]
Armstrong S, Vail A, Mastenbroek S, Jordan V, Farquhar C, Reply: time-lapse in the IVF lab: how should we assess potential benefit?. HUM REPROD 2015;30 (5):1277 [PubMed]
Armstrong S, Vail A, Mastenbroek S, Jordan V, Farquhar C, Time-lapse in the IVF-lab: how should we assess potential benefit?. HUM REPROD 2015;30 (1):3-8 [PubMed]
Kleijkers SHM, Eijssen LMT, Coonen E, Derhaag JG, Mantikou E, Jonker MJ, Mastenbroek S, Repping S, Evers JLH, Dumoulin JCM, van Montfoort APA, Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media. HUM REPROD 2015;30 (10):2303-2311 [PubMed]
Vissenberg R, Manders VD, Mastenbroek S, Fliers E, Afink GB, Ris-Stalpers C, Goddijn M, Bisschop PH, Pathophysiological aspects of thyroid hormone disorders/thyroid peroxidase autoantibodies and reproduction. HUM REPROD UPDATE 2015;21 (3):378-387 [PubMed]
Youssef MMA, Mantikou E, van Wely M, van der Veen F, Al-Inany HG, Repping S, Mastenbroek S, Culture media for human pre-implantation embryos in assisted reproductive technology cycles. COCHRANE DB SYST REV 2015;2015 (11):CD007876 [PubMed]
Mastenbroek S, Repping S, Preimplantation genetic screening: back to the future. HUM REPROD 2014;29 (9):1846-1850 [PubMed]
Wong KM, Mastenbroek S, Repping S, Cryopreservation of human embryos and its contribution to in vitro fertilization success rates. FERTIL STERIL 2014;102 (1):19-26 [PubMed]
Wong KM, Repping S, Mastenbroek S, Limitations of embryo selection methods. SEMIN REPROD MED 2014;32 (2):127-133 [PubMed]
Beukers F, van der Heide M, Middelburg KJ, Cobben JM, Mastenbroek S, Breur R, van der Lee JH, Hadders-Algra M, Bos AF, Kok JH [Contributors: Cobben JM, van der Heide M, Houtzager BA , Kok JH, Mastenbroek S, Repping S, Twisk M, van der Veen F, Bos AF, Haadsma M, Hadders-Algra M, Heineman MJ, van Hoften J, Jongbloed-Pereboom M, Keating P, Middelburg K, Seggers J]] , Morphologic abnormalities in 2-year-old children born after in vitro fertilization/intracytoplasmic sperm injection with preimplantation genetic screening: follow-up of a randomized controlled trial. FERTIL STERIL 2013;99 (2):408-413 [PubMed]
Mantikou E. Improving embryo quality in assisted reproduction. s.l.: s.n.; 2013. 181p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Repping S; copromotor(s): Mastenbroek S)
Mantikou E, Bontekoe S, van Wely M, Seshadri S, Repping S, Mastenbroek S, Low oxygen concentrations for embryo culture in assisted reproductive technologies. HUM REPROD UPDATE 2013;19 (3):209 [PubMed]
Mantikou E, van Echten-Arends J, Sikkema-Raddatz B, van der Veen F, Repping S, Mastenbroek S, Temporal and developmental-stage variation in the occurrence of mitotic errors in tripronuclear human preimplantation embryos. BIOL REPROD 2013;89 (2):42 [PubMed]
Mantikou E, Youssef MAFM, van Wely M, van der Veen F, Al-Inany HG, Repping S, Mastenbroek S, Embryo culture media and IVF/ICSI success rates: a systematic review. HUM REPROD UPDATE 2013;19 (3):210-220 [PubMed]
Mastenbroek S, One swallow does not make a summer. FERTIL STERIL 2013;99 (5):1205-1206 [PubMed]
Mastenbroek S, Twisk M, van der Veen F, Repping S, Preimplantation genetic screening: a systematic review and meta-analysis of RCTs : Erratum. HUM REPROD UPDATE 2013;19 (2):206
Beukers F, Houtzager BA, Paap MCS, Middelburg KJ, Hadders-Algra M, Bos AF, Kok JH [Contributors: Beukers F, Cobben JM, van der Heide M, Houtzager BA , Kok JH, Koomen A, Repping S, Silberbusch L, Twisk M, Mastenbroek S, van der Veen F, Bos AF, Haadsma M, Hadders-Algra M, Heineman MJ, van Hoften J, Jongbloed-Pereboom M, Keating P, Middelburg K, Seggers J]] , Parental psychological distress and anxiety after a successful IVF/ICSI procedure with and without preimplantation genetic screening: Follow-up of a randomised controlled trial. EARLY HUM DEV 2012;88 (9):725-730 [PubMed]
Bontekoe S, Mantikou E, van Wely M, Seshadri S, Repping S, Mastenbroek S, Low oxygen concentrations for embryo culture in assisted reproductive technologies. COCHRANE DB SYST REV 2012;2012 (7):CD008950 [PubMed]
Mantikou E, Wong KM, Repping S, Mastenbroek S, Molecular origin of mitotic aneuploidies in preimplantation embryos. BBA-MOL BASIS DIS 2012;1822 (12):1921-1930 [PubMed]
Musters AM, van Wely M, Mastenbroek S, Kaaijk EM, Repping S, van der Veen F, Mochtar MH, The effect of recombinant LH on embryo quality: a randomized controlled trial in women with poor ovarian reserve. HUM REPROD 2012;27 (1):244-250 [PubMed]
Mastenbroek S. Preimplantation genetic screening. A reappraisal. s.l.: s.n.; 2011. 127p. [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): van der Veen F, Repping S; copromotor(s): Heineman MJ, Bossuyt PM)
Mastenbroek S, Twisk M, van der Veen F, Repping S, Preimplantation genetic screening: a systematic review and meta-analysis of RCTs. HUM REPROD UPDATE 2011;17 (4):454-466 [PubMed]
Mastenbroek S, van der Veen F, Aflatoonian A, Shapiro B, Bossuyt P, Repping S, Embryo selection in IVF. HUM REPROD 2011;26 (5):964-966 [PubMed]
Musters AM, Repping S, Korevaar JC, Mastenbroek S, Limpens J, van der Veen F, Goddijn M, Pregnancy outcome after preimplantation genetic screening or natural conception in couples with unexplained recurrent miscarriage: a systematic review of the best available evidence. FERTIL STERIL 2011;95 (6):2153-2157 [PubMed]
van Echten-Arends J, Mastenbroek S, Sikkema-Raddatz B, Korevaar JC, Heineman MJ, van der Veen F, Repping S, Chromosomal mosaicism in human preimplantation embryos: a systematic review. HUM REPROD UPDATE 2011;17 (5):620-627 [PubMed]
Mastenbroek S, Scriven P, Twisk M, Viville S, van der Veen F, Repping S, What next for preimplantation genetic screening? More randomized controlled trials needed?. HUM REPROD 2008;23 (12):2626-2628 [PubMed]
Mastenbroek S, Twisk M, van der Veen F, Repping S, Preimplantation genetic screening. REPROD BIOMED ONLINE 2008;17 (2):293; author reply 294-293; author reply 295 [PubMed]
Musters AM, Goddijn M, Korevaar JC, van der Veen F, Mastenbroek S, Repping S, Benefits of PGD in patients with recurrent miscarriages?. FERTIL STERIL 2008;90 (1):240-241 [PubMed]
Twisk M, Mastenbroek S, Hoek A, Heineman MJ, van der Veen F, Bossuyt PM, Repping S, Korevaar JC, No beneficial effect of preimplantation genetic screening in women of advanced maternal age with a high risk for embryonic aneuploidy. HUM REPROD 2008;23 (12):2813-2817 [PubMed]
Mastenbroek S, Twisk M, van Echten-Arends J, Sikkema-Raddatz B, Korevaar JC, Verhoeve HR, Vogel NEA, Arts EGJM, de Vries JWA, Bossuyt PM, Buys CHCM, Heineman MJ, Repping S, van der Veen F, In vitro fertilization with preimplantation genetic screening. NEW ENGL J MED 2007;357 (1):9-17 [PubMed]
Mastenbroek S, van der Veen F, Repping S, In vitro fertilization with preimplantation genetic screening - Reply. NEW ENGL J MED 2007;357 (17):1770-1771
Twisk M, Haadsma ML, van der Veen F, Repping S, Mastenbroek S, Heineman MJ, Bossuyt PMM, Korevaar JC, Preimplantation genetic screening as an alternative to prenatal testing for Down syndrome: preferences of women undergoing in vitro fertilization/intracytoplasmic sperm injection treatment. FERTIL STERIL 2007;88 (4):804-810 [PubMed]
Mastenbroek S, Goddijn M, van der Veen F, Repping S, Bossuyt PMM, Korevaar JC, Twisk M, Heineman MJ, PGD--a model to evaluate efficacy?. FERTIL STERIL 2006;85 (2):534-5; author reply 535-6 [PubMed]
Twisk M, Mastenbroek S, van Wely M, Heineman MJ, van der Veen F, Repping S, Preimplantation genetic screening for abnormal number of chromosomes (aneuploidies) in in vitro fertilisation or intracytoplasmic sperm injection. COCHRANE DB SYST REV 2006;2006 (1):CD005291 [PubMed]
Mastenbroek S, M M Bossuyt P, Heineman MJ, Repping S, van der Veen F, Comment 1 on Staessen et al. (2004). Design and analysis of a randomized controlled trial studying preimplantation genetic screening. HUM REPROD 2005;20 (8):2362-2363 [PubMed]
Twisk M, Mastenbroek S, Bossuyt PMM, Korevaar JC, Heineman MJ, Repping S, van der Veen F, The effectiveness of preimplantation genetic screening. REPROD BIOMED ONLINE 2005;11 (4):519-520 [PubMed]
Mastenbroek S, Engel C, van Echten-Arends J, Sikkema-Raddatz B, van Wassenaer AG, de Vries JWA, Heineman MJ, Repping S, van der Veen F, Preimplantatiegenetische screening op numerieke chromosoomafwijkingen bij embryo's van vrouwen van 35 jaar en ouder; de eerste resultaten in Nederland. NED TIJDSCHR GENEESKD 2004;148 (50):2486-2490 [PubMed]
Feenstra MG, Botterblom MH, Mastenbroek S, Dopamine and noradrenaline efflux in the prefrontal cortex in the light and dark period: Effects of novelty and handling and comparison to the nucleus accumbens. NEUROSCIENCE 2000;100 (4):741-748 [PubMed]
Key publications

Mastenbroek S, Twisk M, van Echten-Arends J, Sikkema-Raddatz B, Korevaar JC, Verhoeve HR, Vogel NEA, Arts EGJM, de Vries JWA, Bossuyt PM, Buys CHCM, Heineman MJ, Repping S, van der Veen F, In vitro fertilization with preimplantation genetic screening. NEW ENGL J MED 2007;357 (1):9-17 [PubMed]
Mastenbroek S, Twisk M, van der Veen F, Repping S, Preimplantation genetic screening: a systematic review and meta-analysis of RCTs. HUM REPROD UPDATE 2011;17 (4):454-466 [PubMed]
Kleijkers SHM, Mantikou E, Slappendel E, Consten D, van Echten-Arends J, Wetzels AM, van Wely M, Smits LJM, van Montfoort APA, Repping S, Dumoulin JCM, Mastenbroek S, Influence of embryo culture medium (G5 and HTF) on pregnancy and perinatal outcome after IVF: a multicenter RCT. HUM REPROD 2016;31 (10):2219-2230 [PubMed]
Mantikou E, Youssef MAFM, van Wely M, van der Veen F, Al-Inany HG, Repping S, Mastenbroek S, Embryo culture media and IVF/ICSI success rates: a systematic review. HUM REPROD UPDATE 2013;19 (3):210-220 [PubMed]
Mantikou E, Jonker MJ, Wong KM, van Montfoort APA, de Jong M, Breit TM, Repping S, Mastenbroek S, Factors affecting the gene expression of in vitro cultured human preimplantation embryos. HUM REPROD 2016;31 (2):298-311 [PubMed]


All Publications

Research programmes

Reproduction & Development - Human Reproduction - Clinical Embryology

  Our research focusses on clinical and basic aspects of Human Reproductive Biology.

Faculty
Dr. S. Mastenbroek PhD

PhD Students
Drs. R.P. Berkhout MD
M.A.J. Smits
M. Tarahomi MD

Prof. dr. S. Repping (Reproduction & Development -- Human Reproduction)

Prof. dr. F. van der Veen (Reproduction and development])

Other research related activities
  • Board of directors / Trustees / Supervisory board, Stichting Automatisering Fertiliteit (SAF)
  • Board of directors / Trustees / Supervisory board, Working group lab ´Landelijk Specialistisch Fertiliteits Dossier´
  • Board of directors / Trustees / Supervisory board, Dutch Society for Obstetrics and Gynaecology - SIG ART
  • Membership of editorial board / Editorship, COCHRANE DATABASE OF SYSTEMATIC REVIEWS
  • Membership of medical or scientific committee, Society for Clinical Embryology, Scientific Committee
Current research funding
  • AMC
  • Amsterdam Reproduction and Development