Information about the course Advanced Microscopy.
Goal:
This course is organized by the core facility Cellular Imaging of the AMC and the AO|2M facility of the VUmc, and provides an in-depth view of specific advanced imaging techniques used at imaging centers of AMC and VUmc.
Format:
This course runs once a year for three consecutive days. The course focuses fully on intensive hands-on practical sessions and interactive discussions with experts in the various advanced fluorescence microscope techniques. On the final day participants present their own research topic and discuss which techniques should be implemented in their project.
Content:
The course will take place at the AMC (core facility Cellular Imaging) and the AO|2M facility of the VUmc (O2 building) with also lectures and demonstrations from other advanced microscopy groups in Amsterdam. The participants will focus in depth on specific advanced imaging techniques, including:
- Measuring intra- and inter-molecular protein interactions using FRET and FLIM.
- Confocal microscopy methods (CLSM).
- Super resolution microscopy.
- Confocal quality control.
- Correlative microscopy (bridging fluorescence microscopy and electron microscopy).
- Advanced electron microscopy.
- Usage of fluorophores such as FlAsH/ReAsH.
Scheduled dates:
Spring 2021.
Target audience:
PhD candidates and postdocs. An introduction course like the Basic Microscopy Course is a prerequisite for the Advanced Microscopy Course.
Study load:
44 hours, which is comparable to 1.6 ECTS points.
Course organizers and registration:
Prof. E.A. Reits / Department of Medical Biology AMC / e.a.reits@amsterdamumc.nl / tel. +31 (0)20 - 566 6259.
Rene Musters / AO|2M Scientific Director & Core Facility Manager / r.musters@vumc.nl.
Contact:
AMC Graduate School
E-mail: graduateschool@amc.uva.nl
Tel: +31 (0)20 - 566 3108