Information about the course Cellular Imaging: From Pixel to Publication.
Introduction:
In recent years, Electron Microscopy (EM) has rapidly developed as a powerful research tool for high resolution imaging, leading to high ranking papers, a Nobel price and recently in The Netherlands to a big NWO award. New, more powerful electron microscopes and the possibility to work at very low temperatures (cryo-EM), made it possible to achieve atomic resolutions. Also combined light and electron microscopy (CLEM; correlative microscopy) now allows precise detection of fluorescently labelled molecules in high resolution images of cells and tissues.
Goal:
After this course, you will have insight into the possibilities of electron microscopy as a research tool and are able to decide whether you should use SEM, TEM or CLEM for your analysis. You will be able to operate an EM at basic level, make images and perform basic techniques of sample preparation. You will need some understanding of microscopes in general, but it is not necessary to have experience with EM.
Format:
5 day course: 1 shared general introduction, then split the group in 2 with 8 at WUR, 8 at AMC with expertise both labs.
Content:
During this course, you will be introduced to all these novel developments. You will learn to use both transmission (TEM) as well as scanning electron microscopes (SEM) with different detectors. Perform classical techniques for specimen preparation, like resin embedding of cells and tissues and negative staining of small structures like virus particles, as well as cryo-techniques for both TEM and SEM. The course also includes tips and tricks to perform CLEM.
Scheduled dates:
23 thru 27 September 2019
Target audience:
AMC and WUR PhD candidates
Study load:
44 hours, which is comparable with 1.6 ECTS points
Course organizers:
Jan van Lent, Marcels Giesbers (WUR), Nicole van der Wel, Henk van Veen (AMC)
Application and more information:
For more information about the course please contact: Nicole van der Wel, Department of Medical Biology, e-mail: n.n.vanderwel@amsterdamumc.nl, or Jan van Lent, e-mail: jan.vanlent@wur.nl. For registration please contact Claudia Rhebergen, e-mail: c.rhebergen@amsterdamumc.nl tel. +31 (0)20 566 4950.
Contact:
AMC Graduate School
E-mail: graduateschool@amc.uva.nl
Tel: +31 (0)20 - 566 3108