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J.E.J. Guikema PhD

Position
Scientific staff member (UD)
Main activities
Research
Specialisation
Molecular biology of immunoglobulin gene recombinations
Focus of research

Our research is focused on immunoglobulin gene recombinations in B cells. We aim to understand the basic mechanisms of these recombinations, and the contribution to genomic instability in malignant leukemias and lymphomas. We are especially interested in the generation and resolution of DNA double-strand breaks during immunoglobulin gene recombinations. To that end, we use genome-wide screening approaches to detect DNA breaks in developing B cells that arise as 'collateral damage' of RAG1/2-mediated V(D)J recombinations, and in activated mature B cells during AID-dependent somatic hypermutations and class switch recombination. 

Key publications
  • Hoogeboom R, van Kessel KPM, Hochstenbach F, Wormhoudt TA, Reinten RJA, Wagner K, Kater AP, Guikema JEJ, Bende RJ, van Noesel CJM, A mutated B cell chronic lymphocytic leukemia subset that recognizes and responds to fungi. J EXP MED 2013;210 (1):59-70 [PubMed]
  • Staszewski O, Baker RE, Ucher AJ, Martier R, Stavnezer J, Guikema JEJ, Activation-induced cytidine deaminase induces reproducible DNA breaks at many non-Ig Loci in activated B cells. MOL CELL 2011;41 (2):232-242 [PubMed]
  • Guikema JEJ, Gerstein RM, Linehan EK, Cloherty EK, Evan-Browning E, Tsuchimoto D, Nakabeppu Y, Schrader CE, Apurinic/Apyrimidinic endonuclease 2 is necessary for normal B cell development and recovery of lymphoid progenitors after chemotherapeutic challenge. J IMMUNOL 2011;186 (4):1943-1950 [PubMed]
  • Stavnezer J, Guikema JEJ, Schrader CE, Mechanism and regulation of class switch recombination. ANNU REV IMMUNOL 2008;26:261-292 [PubMed]
  • Guikema JEJ, Linehan EK, Tsuchimoto D, Nakabeppu Y, Strauss PR, Stavnezer J, Schrader CE, APE1- and APE2-dependent DNA breaks in immunoglobulin class switch recombination. J EXP MED 2007;204 (12):3017-3026 [PubMed]
All Publications
Curriculum Vitae

 NAME
Jeroen E.J. Guikema, Ph.D.

TITLE
Assistant Professor (tenure track), AMC Fellow
Academic Medical Center, University of Amsterdam, The Netherlands

ADDRESS
Department of Pathology
Academic Medical Center, University of Amsterdam
Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands
Phone: +31-20-566-5708
Fax: +31-20-566-9523
E-mail: J.E.Guikema@amc.uva.nl

EDUCATION
•2004: Ph.D. Departments of Cell Biology and Hematology, University Medical Center Groningen, University of Groningen, The Netherlands. Thesis title: ‘Heterogeneity of Clonal B cells in Multiple Myeloma’
•1998: M.Sc. Medical and Molecular Biology, University of Groningen, The Netherlands, cum laude

POSITIONS
•2010–present: Assistant Professor (tenure track), AMC Fellow
Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands
•2005–2010: Postdoctoral Fellow with Prof. Janet Stavnezer, Department of Microbiology and Molecular Genetics, University of Massachusetts, Worcester, MA, USA
•2004–2005: Postdoctoral Fellow with Prof. Philip Kluin, Department of Pathology, University Medical Center Groningen, The Netherlands

INTERNATIONAL ACTIVITIES
•Postdoctoral fellow in the lab of Prof. Janet Stavnezer at the University of Massachusetts Medical School, Worcester, MA, USA: October 2005-March 2010.
•Rotation project in the lab of Prof. Kris Thielemans at the Free University of Brussels, Belgium, 7 months: August 1996-March 1997.

OTHER ACADEMIC ACTIVITIES
•Chairman of the ‘Malignant Lymphoma’ subcommittee of the Dutch Cancer Society (KWF).
•Member of the Dutch Hematology Congress (NVvH) program committee.
•Co-organizer of the biweekly ‘Amsterdam Immunology Meeting’ (AIM) at the Academic Medical Center, Amsterdam.
•Ad hoc reviewer for Journal of Immunology, Journal of Experimental Medicine, British Journal of Haematology, Genes Chromosomes and Cancer.

NON-ACADEMIC ACTIVITIES
•Scientific consultant and member of the scientific advisory board at ‘ImmunoQuality Therapeutics Corporation’ based in Groningen, The Netherlands.

MEMBERSHIPS
•American Association of Immunologists (AAI)
•Dutch Society for Immunology (NVVI)
•Dutch Society for Hematology (NVvH)

SCHOLARSHIPS AND PRIZES

  • 2012-2017: NWO-VIDI €800,000; 1/10/2012-31/09/2017
  • 2010-2015: Amsterdam Academic Medical Center Fellowship Award €700,000; 1/04/2010-31/3/2015
  • 2007-2009: Cancer Research Institute Award $130,500; 1/01/2007-12/31/2009

 

Research programmes

Prof. dr. C.J.M. van Noesel (B cell biology and the pathogenesis of B-NHL/ The serrated neoplasia pathway to colorectal cancer)

Other research related activities
  • Membership of advisory board / Consultant, IQ Therapeutics
Current research funding
  • AMC
  • AMC (Vrijgesteld)
  • Europese Unie
  • KWF Kankerbestrijding
  • Stichting KIKA