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A.T. Das PhD

Position
Associate Professor
Main activities
Research
Specialisation
Molecular biology of human immunodeficiency virus (HIV)
Focus of research

HIV replication and anti-viral strategies

This research line includes both basic and applied HIV research, which are intermingled and complement each other.

Basic research focuses on:
1. The structure and function of the HIV genome. In particular, we investigate the structure of the viral RNA and its role in different replication steps, such as transcription, RNA splicing, dimerization and packaging.
2. The role of the viral Tat protein in HIV replication.

Applied research focuses on:
1. Novel strategies to inhibit HIV replication (based on the CRISPR/Cas9 system and RNA interference mechanism).
2. Development of conditionally replicating HIV variants as a novel approach toward a safe live-attenuated HIV vaccine and as a tool to study the host (immune) responses that correlate with protection induced by live-attenuated virus vaccines.
3. The use of viral evolution as a tool to develop new gene expression systems (Tet-On systems).
 

Key publications
  • Harwig Alex, Jongejan Aldo, van Kampen Antoine H. C., Berkhout Ben, Das Atze T. Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA Nucleic acids research 2016;44 (9):4340-4353 [PubMed]
  • Wang Gang, Zhao Na, Berkhout Ben, Das Atze T. CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape Molecular therapy 2016;24 (3):522-526 [PubMed]
  • Wang Gang, Zhao Na, Berkhout Ben, Das Atze T. A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures Cell reports 2016;17 (11):2819-2826 [PubMed]
All Publications