PhD D.M.J. Milstein

Research Associate
Main activities
Oral & Maxillofacial Diagnostics, Translational Research
Focus of research

Research in cardiovascular physiology, surgery, and oral & maxillofacial specialties with a particular focus on tissue oxygenation, microcirculation, (orapathology, regeneration, and oral diagnostic sciences. Research projects include studies in hyperbaric medicine for wound healing, ischemia-reperfusion injuries, osteoradionecrosis & medication-related osteonecrosis of the jaw, photodynamic therapy for head & neck malignancies, clinical value of monitoring perioperative patient microcirculation, and evaluating the influences of both oral prosthesis and their materials on normal and pathological lesions.


Key publications
  • Tytgat Stefaan H. A. J., van der Zee David C., Ince Can, Milstein Dan M. J. Carbon dioxide gas pneumoperitoneum induces minimal microcirculatory changes in neonates during laparoscopic pyloromyotomy Surgical endoscopy 2013;27 (9):3465-3473 [PubMed]
  • Milstein Dan M. J., van Kuijen Anne-Marije, Copper Marcel P., Karakullukçu Barış, Tan I. Bing, Lindeboom Jérôme A. H., Fokkens Wytske J., Ince Can Monitoring microcirculatory alterations in oral squamous cell carcinoma following photodynamic therapy Photodiagnosis and photodynamic therapy 2012;9 (1):69-75 [PubMed]
  • Milstein Dan M. J., te Boome Liane C. J., Cheung Yuk Wah, Lindeboom Jérôme A. H., van den Akker Hans P., Biemond Bart J., Ince Can Use of sidestream dark-field (SDF) imaging for assessing the effects of high-dose melphalan and autologous stem cell transplantation on oral mucosal microcirculation in myeloma patients Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2010;109 (1):91-97 [PubMed]
  • Milstein Dan M. J., Bezemer Rick, Lindeboom Jérôme A. H., Ince Can The acute effects of CMF-based chemotherapy on maxillary periodontal microcirculation Cancer chemotherapy and pharmacology 2009;64 (5):1047-1052 [PubMed]
  • Milstein Dan M. J., Mathura Keshen R., Lindeboom Jerôme A. H., Ramsoekh Dewkoemar, Lindeboom Robert, Ince Can The temporal course of mucoperiosteal flap revascularization at guided bone regeneration-treated implant sites: a pilot study Journal of clinical periodontology 2009;36 (10):892-897 [PubMed]
All Publications
Research programmes

Prof. PhD C. Ince (Cardiovascular aspects of Surgery and Critical Illness)

Our research group has three main lines of research; 1)Clinical microcirculation research in perioperative phase 2)The red blood cell in critical illnes and surgery, 3)Experimental investigations into microcirculatory and mitochondrial dysfunction in sepsis, shock and resuscitation. Our research is translational in nature where we develop concepts and techniques fron bench-to-bedside. In theme 1 we identified 5 different classes of microcirculatory alterations in distributive shock. We identified microcirculatory recruitment maneuvers in the treatment of sepsis, and developed microcirculation research in cardiac and maxillofacial surgery. In theme 2 we investigated how storage affects the quality of blood transfusions. We found that fluid resuscitation causes enhancement in renal  oxygen consumption and redistribution of oxygen transport. This line of research resulted in a grant awarded by the Landsteiner Foundation for Blood Transfusion Research. In theme 3 we developed a two-wave length technique for measuring the redistribution of tissue oxygenation in organs. Our year was rounded off by our publication in Nature Methods where we identified an endogenous mitochondrial molecule, protoporyphrin IX, with which, using quenching of delayed fluorescence, mitochondrial pO2 can be measured quantitatively  in vivo. This new technique is expected to provide important new insights into the nature of mitochondrial dysfunction in critical illness.

Theme: Cardiovascular Diseases

MSc B. Ergin

MD PhD E.C. Boerma
PhD D.M.J. Milstein

PhD Students
P. Guerci
H.W. He

Y. Ince

PhD D.M.J. Milstein (Promoties)