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Dr. G.T.M.L. Oei BSc MD PhD

Position
Postdoc
Main activities
Patient care, Research
Specialisation
Anesthesiology, cardiovascular research
Focus of research

Cardioprotection by noble gases and inhaled anesthetics

Anesthetic gases such as sevoflurane, desflurane and isoflurane but also xenon have organ protective properties. Possible mechanisms include signaling kinases affecting several end effectors such as mitochondria and mitondrial channels. The complex mechanism underlying anesthetic-induced cardioprotection resemble the mechanisms underlying ischemic pre- and postconditioning. For the clinic, ischemic conditioning is not so much an attractive option, as it can induce significant organ damage itself.

The non-anesthetic noble gas helium can also protect the heart against episodes of ischemia/reperfusion. Application of 15 minutes of helium 24 hours before or directly after the index ischemia induces late preconditioning and postconditioning respectively. In our line of research we focus on the mechanism of action behind helium induced conditioning in the heart. We are currently investigating the transcriptional effects of helium inhalation on genes involved in cell death and -survival. We also investigate the role of helium conditioning and caveolin.

Another important aspect of our research is the protection of the diseased rat, in experimental models of diabetes and hypertension we have investigated the effectiveness of helium induced cardioprotection. Finally, we investigate whether helium can exert protective effects in humans and whether it is safe. In the future, more studies in human subjects will follow, in which possible mechanisms of action will be investigated.

Key publications
  • Oei GTML, Weber NC, Hollmann MW, Preckel B, Cellular Effects of Helium in Different Organs. ANESTHESIOLOGY 2010;112 (6):1503-1510 [PubMed]
  • Huhn R, Heinen A, Weber NC, Kerindongo RP, Oei GTML, Hollmann MW, Schlack W, Preckel B, Helium-induced early preconditioning and postconditioning are abolished in obese Zucker rats in vivo. J PHARMACOL EXP THER 2009;329 (2):600-607 [PubMed]
  • Oei GTML, Smit KF, vd Vondervoort D, Brevoord D, Hoogendijk A, Wieland CW, Hollmann MW, Preckel B, Weber NC, Effects of helium and air inhalation on the innate and early adaptive immune system in healthy volunteers ex vivo. J TRANSL MED 2012;10 (1):201 [PubMed]
  • Oei GTML, Huhn R, Heinen A, Hollmann MW, Schlack WS, Preckel B, Weber NC, Helium-induced cardioprotection of healthy and hypertensive rat myocardium in vivo. EUR J PHARMACOL 2012;684 (1-3):125-131 [PubMed]
All Publications
Curriculum Vitae

Professional working experience and internships

• 01/2013 – 04/2014 Onze Lieve Vrouwe Gasthuis, Amsterdam, AIOS Anesthesiologie

• 01/2012 – 12/2012 Academisch Medisch Centrum (AMC) Amsterdam, AIOS Anesthesiologie/ PhD student L.E.I.C.A.

• 08/2011 – 11/2011 Jeroen Bosch Ziekenhuis Den Bosch, ANIOS cardiologie voor diensten/ PhD student L.E.I.C.A.

• 08/2007 – 08/2011 Academic Medical Centre (AMC) Amsterdam, MD/PhD-program; PhD-thesis at the Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A.) ‘Mechanisms of conditioning-induced cardioprotection in healthy and diseased hearts’. Internships of choice: Cardiology, Intensive Care

• 12/2005 – 03/2006 Academic Medical Centre (AMC) Amsterdam, internship Dep. of Medical Microbiology, Parasitology. Cost-effectivity of diagnostic tests for malaria.

• 03/2004 – 05/2004 University Medical Centre Utrecht (UMCU), internship Board of Directors. Coordination of the implementation of an indicator-set for quality of care management.

Education

• 08/2007 – 12/2013 University of Amsterdam,  Dissertation: 12/2013

• 09/2004 – 02/2008 University of Amsterdam, Master of Science in Medicine. Graduation: 02/2008

• 09/2001 – 06/2004 Erasmus University Rotterdam, Bachelor of Science in Health Care Sciences. Graduation: 06/2004.


Grants/Scholarships/prizes

• 2011 Spinoza beurs voor Annual Meeting American Society of Anesthesiologists, October 2010, San Diego CA, USA (€560,00)

• 2010 Nederlandse Vereniging voor Anesthesiologie, eerste prijs (€350,00) voor voordracht ‘The effect of helium on the immune system ex vivo’

• 2010 Spinoza beurs voor Cursus Microchirurgie in rat en muis, Wake Forest University, USA (€1524,00)

• 2008-2009 Personal scholarship for research activities: Charlotte Jacobs Studiefonds, € 900,00 at once and € 100 a month for 16 months.

• 2008 Society of Cardiovascular Anesthesiologists (SCA), 2008 Foundation Starter Grant for project ‘Does the Noble Gas Helium Protect the Myocardium in State of Hypertension in vivo?’

• 2008 Nederlandse Hartstichting, derde prijs (€200,00) voor voordracht ‘Protective effects of helium-induced POSTconditioning in the rat heart in vivo cannot be enlarged by addition of helium-induced early- (EPC) or late PREconditioning (LPC)’

• 2007 Personal scholarship for research activities: Hendrik Muller’s Vaderlandsch Fonds: € 2000,00

Skills

Computer skills: SPSS, Prism, Labchart, Bookends (Mac), Adobe photoshop/indesign

Research techniques and models:
• Molecular techniques : Western blot, EMSA, CBA, ELISA
• Models: immune stimulation of whole blood ex vivo, Langendorff and in vivo ischemia/reperfusion rat model
• Miscellaneous: intrathoracic echocardiography, pressure-volume measurements of left ventricle with conductance catheter.


Conferences and abstracts

Euroanesthesia 2014, European Society of Anesthesiologists, Stockholm, Sweden. Helium postconditioning increases Caveolin 1 and 3 protein levels in serum of rats. Steenstra R.J., Oei G.T.M.L., Preckel B., Hollmann M.W., Patel H., Weber N.C.

Experimental Biology 2013, Federation of American Societies for Experimental Biology, Boston MA, USA. Transcriptional regulation of cardiac cell death and survival signaling by helium postconditioning in a rat model of regional cardiac ischemia/reperfusion. G.T.M.L. Oei, M. Heger, R.F. van Golen, B. Preckel, M.W. Hollmann, N.C.Weber.

Euroanaesthesia 2013, European Society of Anesthesiologists, Barcelona, Spain. Helium induced-cardioprotection and inflammatory cytokines in reperfused rat myocardium in vivo. G.T.M.L. Oei, R.P. Kerindongo, M.W. Hollmann, B. Preckel, N.C. Weber.

American Society of Anesthesiologists Annual Meeting 2012, Washington, USA. Cardioprotection after a short episode of 70% helium inhalation at early reperfusion is abrogated by prolonged inhalation during reperfusion. G.T.M.L. Oei, M.W. Hollmann, B.Preckel, N.C. Weber.

Euranesthesia 2012, European Society of Anesthesiologists Annual Meeting 2012, Paris, France. Effect on innate immunity after regular and prolonged 79% helium inhalation in healthy males. G.T.M.L. Oei, K.F. Smit, B.Preckel, N.C. Weber

Euranesthesia 2011, European Society of Anesthesiologists Annual Meeting 2011, Amsterdam, the Netherlands. Helium-induced conditioning of the hypertensive rat heart. G.T.M.L. Oei, R. Huhn, M.W. Hollmann, W. Schlack, B. Preckel, N.C. Weber

American Society of Anesthesiologists Annual Meeting 2010, San Diego, USA. The effect of helium inhalation on the immune system ex vivo. G.T.M.L.Oei, K.F.Smit, D. v.d. Vondervoort, B.Preckel, N.C.Weber.

Dutch Society of Anesthesiologists, annual meeting 2010, Ede, the Netherlands. The effect of helium on the immune system ex vivo. Oral presentation (first price). G.T.M.L.Oei, K.F.Smit, D. v.d. Vondervoort, B.Preckel, N.C.Weber

Dutch Society of Anesthesiologists, annual meeting 2010, Ede, the Netherlands. Helium induces early and late preconditioning in human endothelium in vivo. K.F.Smit, N.C.Weber, E.S. Stroes, G.T.M.L. Oei, M.W. Hollmann, B.Preckel

International Anesthesia Research Society Annual Meeting 2010, Honolulu, USA. Influence of helium on production of microparticles and its relation to preconditioning in human endothelium. B.Preckel, K.F.Smit, G.T.M.L.Oei, R.Nieuwland, M.W.Hollmann, N.C.Weber

International Anesthesia Research Society Annual Meeting 2010, Honolulu, USA. Helium induces early and late preconditioning in human endothelium in vivo. K.F.Smit, N.C.Weber, E.S. Stroes, G.T.M.L. Oei, M.W. Hollmann, B.Preckel

American Society of Anesthesiologists Annual Meeting 2009, New Orleans, USA. Helium-induced postconditioning in the rat heart in vivo- possible role for GSK3-β and PKC-ε. G.T.M.L. Oei, R. Huhn, M.W. Hollmann, W. Schlack, B. Preckel, N.C. Weber

Euroanesthesia 2009, European Society of Anesthesiologists, Milan, Italy. Helium-induced postconditioning in the rat heart in vivo. G.T.M.L. Oei, R. Huhn, M.W. Hollmann, W. Schlack, B. Preckel, N.C. Weber

Deutsche Anästhesie Kongress 2009, Leipzig, Germany. Helium induziert Postkonditionierung im Rattenherzen in vivo G.T.M.L. Oei, R. Huhn, M.W. Hollmann, W. Schlack, B. Preckel, N.C. Weber

Nederlandse Hartstichting/ Dutch Heart Foundation course 2008, Papendal, the Netherlands. Helium induced preconditioning in human endothelium in vivo. K.F.Smit, G.T.M.L.Oei, N.C.Weber, M.W. Hollmann, B. Preckel

Nederlandse Hartstichting/ Dutch Heart Foundation course 2008, Papendal, the Netherlands. Protective effects of helium-induced POSTconditioning in the rat heart in vivo cannot be enlarged by addition of helium-induced early- (EPC) or late PREconditioning (LPC). Oral presentation (third price). G.T.M.L. Oei, R. Huhn, N.C. Weber, M.W. Hollmann, W.S. Schlack, B. Preckel

Hauptstadtkongress der DGAI für Anästhesiologie und Intensivmedizin 2008, Berlin, Germany. Heliox- ein “neues“ Therapeutikum in der Intensivmedizin. Kardioprotektive Effekte von Helium-Sauerstoff-Mischungen. G.T.M.L. Oei, R. Huhn, K.F.Smit, B.Preckel
 

(Academic) activities

2013 Symposium: Perioperatieve hemostase en bloedstolling, VU Amsterdam
2013 Cursus: Coach de Co, OMSO Amsterdam

2012 Cursus: Advanced Trauma Life Support, Harlem Hospital Centre, NY, USA
2012 Bijscholing: Difficult Airway Management, Annual Meeting ASA, Washington DC, USA
2012 Cursus: BROK/GCP, Graduate School AMC
2012 Cursus: Systematic Reviews, Graduate School AMC
2012 Cursus: Advanced Life Support, ERC, AMC
2012 Cursus: Clinical Data Management, Graduate School AMC

2011 Cursus: BLS en opvang SEH, Jeroen Bosch Ziekenhuis, Den Bosch

2009 Cursus: “Microvascular Hemodynamic Surgery and Survival Surgery Techniques”, Wake Forest University- School of Medicine, Winston-Salem NC, United States
2009 Symposium Pressure-Volume Loop measurements, Scisense
2008 Cursus ‘Practical Biostatistics’, Afdeling Klinische Epidemiologie en Biostatistiek, Graduate School AMC

2008 Cursus ‘Cardiac Functioning and Repair’, Nederlandse Hartstichting
2008 Begeleiding studenten AMC/UvA Summerschool 2008 ‘Heart Failure and Repair’
2008 Cursus ‘Proteomics’, Afdeling Biochemie, Graduate School AMC
2008 Cursus ‘Proefdierkunde’ voor artikel 9 bevoegdheid, Graduate School AMC

 

Research programmes

Prof. dr. B. Preckel MA MD PhD (Patient safety, procedural sedation, cardiovascular protection, optimizing perioperative metabolism, cerebral perfusion, Wireless Monitoring)