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Dr. R. Nieuwland

Position
Scientific staff member (UD)
Main activities
Research, Teaching
Specialisation
Biologist
Focus of research

Detection and function of extracellular vesicles (microparticles, exosomes) and platelets in health and disease.

Key publications
  • van der Pol E, Böing AN, Harrison P, Sturk A, Nieuwland R, Classification, Functions, and Clinical Relevance of Extracellular Vesicles. PHARMACOL REV 2012;64 (3):676-705 [PubMed]
  • Berckmans RJ, Sturk A, van Tienen LM, Schaap MCL, Nieuwland R, Cell-derived vesicles exposing coagulant tissue factor in saliva. BLOOD 2011;117 (11):3172-3180 [PubMed]
  • van der Pol E, Coumans FAW, Sturk A, Nieuwland R, van Leeuwen TG, Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis. NANO LETT 2014;14 (11):6195-6201 [PubMed]
  • Kalra H, Simpson RJ, Ji H, Aikawa E, Altevogt P, Askenase P, Bond VC, Borràs FE, Breakefield X, Budnik V, Buzas E, Camussi G, Clayton A, Cocucci E, Falcon-Perez JM, Gabrielsson S, Gho YS, Gupta D, Harsha HC, Hendrix A, Hill AF, Inal JM, Jenster G, Krämer-Albers EM, Lim SK, Llorente A, Lötvall J, Marcilla A, Mincheva-Nilsson L, Nazarenko I, Nieuwland R, Nolte-'t Hoen ENM, Pandey A, Patel T, Piper MG, Pluchino S, Prasad TSK, Rajendran L, Raposo G, Record M, Reid GE, Sánchez-Madrid F, Schiffelers RM, Siljander P, Stensballe A, Stoorvogel W, Taylor D, Thery C, Valadi H, van Balkom BWM, Vázquez J, Vidal M, Wauben MHM, Yáñez-Mó M, Zoeller M, Mathivanan S, Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation. PLOS BIOL 2012;10 (12):e1001450 [PubMed]
  • van der Pol E, Coumans FAW, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, Sturk A, van Leeuwen TG, Nieuwland R, Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J THROMB HAEMOST 2014;12 (7):1182-1192 [PubMed]
All Publications
Curriculum Vitae

Research

Member of the Executive Board of the International Society on Extracellular Vesicles (www.isev.org)   

Chair of the SSC scientific Subcommittee on Vascular Biology of the International Society on Thrombosis and Haemostasis (www.isth.org)

Coordinator of the European Metrology Research Programme METVES (www.metves.eu)  

 

Education

Lid Colloquium Doctum Commissie (Centrale Commissie Voortentamen Biologie)

Cytometry Development Workshop (San Diego, CA), Oct 2016

ISEV Massive Open Online Course Basics of Extracellular Vesicles (www.isev.org/isev-mooc-basics-of-extracellular-vesicles/), 2016

PhD course on Extracellular Vesicles of the Karolinska Institute (Sweden, Stockholm), 2016

Maastricht Summerschool on Thrombin Generation (Maastricht, Netherlands), June 2016

Invited lectures at Universities of Helsinki (Helsinki, Finland; 2016), Danube (Krems; Austria; 2016),  Warsaw (Warsaw, Poland; 2016), Vienna (Vienna, Austria; 2016), Gent (Gent, Belgium; 2015),Oxford (Oxford, UK; 2015).

Webinar on "Microvesicles: what is their function" (ISTH), Jan 2015

 

 

Research programmes

Extracellular vesicles and platelets in health and disease

All human body fluids contain not only cells but also high concentrations (10E7-10E12/mL) of cell-derived (extracellular) vesicles.The bulk (>80%) of these extracelullar vesicles (EV) are ultra-small, having a diameter < 200 nm. Because (i) the concentration, composition, cellular origin and function of extracellular vesicles (EV) change during disease, and because (ii) EV play an active role in disease development and progression, intercellular communication and protection of cells and even organisms, there is since 2000-2001 an exponentionally growing scientific and clinical interest in EV.


By an intensive and long-standing collaboration with the Department of Biomedical Engineering (Prof. Dr. T.G. van Leeuwen, Dr. F. Coumans, Dr. E. van der Pol), we have now an internationally acknowledged research group with a keen interest on EV detection, isolation and functional characterization. Earlier we coordinated an EU metrology research project focused on detection of EV (www.metves.eu). In 2014, we initiated a Dutch STW-funded perspectief program (M€ 5.8), CANCER-ID, which is a collaboration between 6 Dutch universities and 22 SME's, and aimed at developing one or more platforms to enable the reliable detection and characterization of tumor-derived vesicles in blood.


The output of our multi-disciplinary collaboration is internationally appreciated and acknowledged, as evident from invitations to present keynote/state-of the art lectures (ISTH 2013, ISAC 2015, Gordon Conference 2016), co-founding the international flow cytometry work group of ISEV-ISAC-ISTH (www.evflowcytometry.org), coordinating a first multi-centre trial –the Bouilloud study- on the prediction of venous thromboembolism in cancer patients (800-1,000 patients) by measuring the coagulant activity of EV using an AMC-developed functional EV assay, invitations to contribute to development of online teaching courses (ISEV, ISTH), invitation to contribute to training of students (e.g. Karolinska Institute 2015, CDW Course San Diego 2016), invitation from scientific journals as Circulation Research (American Heart Association) to coordinate a review on isolation and detection of EV (to be published in 2017), etc. Furthermore, we also provide an international student training facility.


Finally, we have a unique central platelet research facility within the AMC, which has been used by >10 different departments within the AMC and VUMC for clinical platelet studies.
 

Faculty
Dr. R. Nieuwland (VSL)

Postdocs
Dr. R.J. Berckmans
Dr. ir. F.A.W. Coumans

PhD Students
N. Buntsma
Ir. E. Gool MSc
Drs. D.L. Horjus
Ir. L. de Rond MSc
Ir. Y. Yu MSc
Drs. L. Rikkert MSc

Others
Ing. N. Hajji
Ing. C.M. Hau BA
Prof. dr. A. Sturk

Current research funding
  • Technologiestichting STW