J.C.J. Calis MD PhD

Medical Specialist
Main activities
Patient care, Research
Pediatric Intensive Care Medicine & Global Child Health
Focus of research

Pediatric critical care in low resource settings

Subtopics include

- Circulatory failure & Severe anaemia

- Severe infectious diseases 


Key publications
  • Calis Job C. J., Phiri Kamija S., Faragher E. Brian, Brabin Bernard J., Bates Imelda, Cuevas Luis E., de Haan Rob J., Phiri Ajib I., Malange Pelani, Khoka Mirriam, Hulshof Paul J. M., van Lieshout Lisette, Beld Marcel G. H. M., teo Yik Y., Rockett Kirk A., Richardson Anna, Kwiatkowski Dominic P., Molyneux Malcolm E., Boele van Hensbroek Michael Severe anemia in Malawian children New England journal of medicine 2008;358 (9):888-899 [PubMed]
  • Calis Job, van Woensel Job, Lemson Joris Severe sepsis and septic shock New England journal of medicine 2013;369 (21):2062 [PubMed]
  • Calis Job C. J. Aetiology, pathogenesis & consequences of severe anaemia in Malawian children. HIV and other factors 2008. 183p. ISBN 9789090228860. [UvA Dissertations Online]
  • Sigaloff Kim C. E., Calis Job C. J., Geelen Sibyl P., van Vugt Michèle, de Wit Tobias F. Rinke HIV-1-resistance-associated mutations after failure of first-line antiretroviral treatment among children in resource-poor regions: a systematic review Lancet infectious diseases 2011;11 (10):769-779 [PubMed]
  • Wiegers Hanke M. G., van Nijen Lisa, van Woensel Job B. M., Bem Reinout A., de Jong Menno D., Calis Job C. J. Bacterial co-infection of the respiratory tract in ventilated children with bronchiolitis; A retrospective cohort study BMC infectious diseases 2019;19 (1) [PubMed]
All Publications
Curriculum Vitae


  • University/College of Higher Education:                 University of Amsterdam

  • Date:                                                                       08-06-2000 (Arts-examen 20-06-2002)



  • University/College of Higher Education:           University of Amsterdam (Cum Laude)

  • Completion date (dd/mm/yy):                           22-05-2008

  • Supervisor (‘Promotor’):                                   Prof. B.J. Brabin

  • Title of thesis:                                                   Aetiology, pathogenesis & consequences of severe anaemia in Malawian children

  • Registration as a Specialist                             13-05-2012 (Pediatrician); 27-03-2015 (Pediatric Intensivist)


Current employment

Present appointment: Pediatrician- intensive care consultant (as of March 2015)


Work experience since completing PhD

Pediatric Registrar                          May 08 - May 12              UvA/EKZ

Fellow Pediatric intensive care    May 12-Feb15                   UvA/EKZ & LUMC

Research Staff                                 May 08- Current              Global Child health group / AIGHD UvA


Supervision of PhD and Master students


PhD Students



Daily supervision, co-promotor

FAM Jonker

Jan 09-Oct 12

Shared supervision, co-promotor

K Sigaloff

Jan 09-Feb 13

Shared supervision, co-promotor

TS Boender

Mar 13-May 16

Shared supervision, co-promotor

R Boerma

Feb 15- Mar 17

Daily supervision, co-promotor

M Huibers

Apr 12-exp 17


Master Students

Daily supervision master

L v Nijen

Jul 13-Oct13

Daily supervision, bachelor & master

H Wiegers

Jan 14-Oct15

Daily supervision, master

L Kemps

Feb 17-Jul 17

Daily supervision, bachelor

I Snik

Oct 17- current

Other Supervision

Coordinator Severe anaemia study,

        Blantyre studysite

6 persons


Weekly supervision

P Moons Paediatrician-PI

Apr 09-Mar12

International activities

Oral presentation; group discussion and lectures include:

  • Annual Wellcome Trust meeting (Liverpool 2003 & 2005, Malawi 2006, Kenya 2007)

  • Tropical Medicine and International Health global conference, Amsterdam 2007

  • NIH Gates Iron-Malaria meeting, Washington 2008

  • Amsterdam University College 2012-current (Shock)

  • Amsterdam Symposium 2013 (septic shock)

  • Paediatric Course lecturer, Medicines Sans Frontieres; Malawi 2013, 2014, 2016

  • DTM&H lecturer; Liverpool School of Tropical Medicine & Hygiene; Liverpool 2014

  • MSF: Ped Scientific Days shock Treatment; Stockholm 2016

    Work visits include:

  • Blantyre (Malawi, Wellcome Trust Research Laboratories) residency 2002-05, visits

  • Kilifi (Kenya; Wellcome Trust Center, Kemri ) 2006

  • Oxford (England; Wellcome Trust Centre for Human Genetics) 2007

  • Lagos/Illorin (Nigeria; Lagos&Illorin University Teaching hospital) visits 2010-11

  • Liverpool (England; Liverpool School of Tropical Medicine) several visits 2005-14


    Other academic activities

    Management activities

  • Spokesman PhD Students, Wellcome Trust Malawi 2003-2005

  • Spokesman paediatric registrars, SLAZ hospital 2008

  • Chief Registrar (Chair of paediatric registrars AMC) 2009-2012



  • Organisation/co-facilitator Tropical child health&paediatrics symposium, Amsterdam 2005

  • Coordinator paediatric training A&E nurses; 2009-2012

  • Lecturer global child health group course AIOS (HIV/TB/research); 2009-current

  • Lecturer Amsterdam University College –PICU 2012-current

  • Coordinator paediatric intensive care training - paediatric registrars; 2011-current

  • Lecturer circulation & upper airway problems; MSF-paediatric course, Malawi 2013-current

  • Lecturer International Child Health Liverpool School of Tropical Medicine 2013








    (Dutch pediatric society)

    Young Investigator Award (2nd)



    PhD-Thesis Award (2nd)



    Best paediatric resident (1st)



    Best paediatrician (nominee)


    Scholarship/Grants PI or co-PI

    Ter Meulen Fonds PhD scholarship: HIV and anaemia;



    Numico stipendium PhD scholarship: HIV and anaemia;



    NWO-NACCAP co-PI: simple markers to start HAART in children;



    St. steun Emma: co-PI simple markers to start HAART in children;



    Janivo: co-PI hepcidin and other iron markers in Malawian children;



    St steun Emma: co-PI hepcidin & other iron markers in children;



    Grant as co-applicant



    Min Foreign Aff: health &insurance in Nigerian Children collaborator



    Numico stipendium PhD scholarship: Iron and anaemia;



2. List of publications

Neutrophil subset responses in infants with severe viral respiratory infection.

Cortjens B, Ingelse SA, Calis JC, Vlaar AP, Koenderman L, Bem RA, van Woensel JB.

Clin Immunol. 2017 Jan 17;176:100-106.


Early Fluid Overload Prolongs Mechanical Ventilation in Children With Viral-Lower Respiratory Tract Disease.

Ingelse SA, Wiegers HM, Calis JC, van Woensel JB, Bem RA.

Pediatr Crit Care Med. 2017 Jan 19.


Alarming increase in pretreatment HIV drug resistance in children living in sub-Saharan Africa: a systematic review and meta-analysis.

Boerma RS, Sigaloff KC, Akanmu AS, Inzaule S, Boele van Hensbroek M, Rinke de Wit TF, Calis JC.

J Antimicrob Chemother. 2017 Feb;72(2):365-371.


Severe anemia in Malawian children.

Calis JC, Phiri KS, Faragher EB, Brabin BJ, Bates I, Cuevas LE, de Haan RJ, Phiri AI, Malange P, Khoka M, Hulshof PJ, van Lieshout L, Beld MG, Teo YY, Rockett KA, Richardson A, Kwiatkowski DP, Molyneux ME, van Hensbroek MB.

Malawi Med J. 2016 Sep;28(3):99-107.


High levels of pre-treatment HIV drug resistance and treatment failure in Nigerian children.

Boerma RS, Boender TS, Sigaloff KC, Rinke de Wit TF, van Hensbroek MB, Ndembi N, Adeyemo T, Temiye EO, Osibogun A, Ondoa P, Calis JC, Akanmu AS.

J Int AIDS Soc. 2016 Nov 10;19(1):21140.


Suboptimal Viral Suppression Rates Among HIV-Infected Children in Low- and Middle-Income Countries: A Meta-analysis.

Boerma RS, Boender TS, Bussink AP, Calis JC, Bertagnolio S, Rinke de Wit TF, Boele van Hensbroek M, Sigaloff KC.

Clin Infect Dis. 2016 Dec 15;63(12):1645-1654.


Second-line HIV Treatment in Ugandan Children: Favorable Outcomes and No Protease Inhibitor Resistance.

Boerma RS, Kityo C, Boender TS, Kaudha E, Kayiwa J, Musiime V, Mukuye A, Kiconco M, Nankya I, Nakatudde L, Mugyenyi PN, Boele van Hensbroek M, Rinke de Wit TF, Sigaloff KC, Calis JC.

J Trop Pediatr. 2016 Sep 15.


Accumulation of HIV-1 drug resistance after continued virological failure on first-line ART in adults and children in sub-Saharan Africa.

Boender TS, Kityo CM, Boerma RS, Hamers RL, Ondoa P, Wellington M, Siwale M, Nankya I, Kaudha E, Akanmu AS, Botes ME, Steegen K, Calis JC, Rinke de Wit TF, Sigaloff KC.

J Antimicrob Chemother. 2016 Oct;71(10):2918-27.


HIV drug resistance among children initiating first-line Antiretroviral Treatment (ART) in Uganda.

Kityo C, Sigaloff KC, Boender ST, Kaudha E, Kayiwa J, Musiime V, Mukuye A, Kiconco M, Nankya I, Nakatudde L, Calis JC, Rinke de Wit TF, Mugyenyi P.

AIDS Res Hum Retroviruses. 2016 Jul;32(7):628-35


An Evaluation of Alternative Markers to Guide Initiation of Anti-retroviral Therapy in HIV-Infected Children in Settings where CD4 Assays are not Available.

Huibers MH, Moons P, Maseko N, Gushu MB, Wit FW, Graham SM, van Hensbroek MB, Calis JC.

J Trop Pediatr. 2016 Feb;62(1):19-28


The Impact of Rhinovirus Infections in Paediatric Cardiac Surgery (Risk): Study Protocol for a Prospective Cohort Study

van Rijn AL, Roeleveld PP, de Wilde RBP, van Zwet EW, Hazekamp MG, Wink J, Calis JCJ, Kroes ACM,  de Vries JJC

J Clin Trials 2015, 5: 246


Conventional and novel peripheral blood iron markers compared against bone marrow in Malawian children.

Jonker FA, Boele van Hensbroek M, Leenstra T, Vet RJ, Brabin BJ, Maseko N, Gushu MB, Emana M, Kraaijenhagen R, Tjalsma H, Swinkels DW, Calis JC.

J Clin Pathol. 2014 Aug;67(8):717-23.


The interaction between malaria and human immunodeficiency virus infection in severely anaemic Malawian children: a prospective longitudinal study.

Kyeyune FX, Calis JC, Phiri KS, Faragher B, Kachala D, Brabin BJ, van Hensbroek MB.

Trop Med Int Health. 2014 Jun;19(6):698-705.


Low hepcidin levels in severely anemic malawian children with high incidence of infectious diseases and bone marrow iron deficiency.

Jonker FA, Calis JC, Phiri K, Kraaijenhagen RJ, Brabin BJ, Faragher B, Wiegerinck ET, Tjalsma H, Swinkels DW, van Hensbroek MB.

PLoS One. 2013 Dec 5;8(12):e78964.


Severe sepsis and septic shock.

Calis J, van Woensel J, Lemson J.

N Engl J Med. 2013 Nov 21;369(21):2062.


Short communication: high rates of thymidine analogue mutations and dual-class resistance among HIV-infected Ugandan children failing first-line antiretroviral therapy.

Sigaloff KC, Kayiwa J, Musiime V, Calis JC, Kaudha E, Mukuye A, Matama C, Nankya I, Nakatudde L, Dekker JT, Hamers RL, Mugyenyi P, Rinke De Wit TF, Kityo C.

AIDS Res Hum Retroviruses. 2013 Jun;29(6):925-30.


Iron status predicts malaria risk in Malawian preschool children.

Jonker FA, Calis JC, van Hensbroek MB, Phiri K, Geskus RB, Brabin BJ, Leenstra T.

PLoS One. 2012;7(8):e42670.


Iron deficiency in children with HIV-associated anaemia: a systematic review and meta-analysis.

Esan MO, Jonker FA, Hensbroek MB, Calis JC, Phiri KS.

Trans R Soc Trop Med Hyg. 2012 Oct;106(10):579-87.


Real-time PCR demonstrates Ancylostoma duodenale is a key factor in the etiology of severe anemia and iron deficiency in Malawian pre-school children.

Jonker FA, Calis JC, Phiri K, Brienen EA, Khoffi H, Brabin BJ, Verweij JJ, van Hensbroek MB, van Lieshout L.

PLoS Negl Trop Dis. 2012;6(3):e1555.


Barriers to Initiation of Pediatric HIV Treatment in Uganda: A Mixed-Method Study.

Boender TS, Sigaloff KC, Kayiwa J, Musiime V, Calis JC, Hamers RL, Nakatudde LK, Khauda E, Mukuye A, Ditai J, Geelen SP, Mugyenyi P, Rinke de Wit TF, Kityo C.

AIDS Res Treat. 2012;2012:817506.




HIV-1-resistance-associated mutations after failure of first-line antiretroviral treatment among children in resource-poor regions: a systematic review.

Sigaloff KC, Calis JC, Geelen SP, van Vugt M, de Wit TF.

Lancet Infect Dis. 2011 Oct;11(10):769-79.


JCJ Calis, KS Phiri, RJWM Vet, RJ de Haan, F Munthali, RJ Kraaijenhagen, PJM Hulshof, ME Molyneux, BJ Brabin, M Boele van Hensbroek, I Bates. Erythropoiesis in HIV-infected and uninfected Malawian children with severe anemia.

AIDS 2010 Nov 27;24(18):2883-7.


M. Boele van Hensbroek, JCJ Calis, KS Phiri, RJWM Vet, F Munthali, R Kraaijenhagen, H van den Berg, B Faragher, I Bates, ME Molyneux. Pathophysiological Mechanisms of Severe Ane-mia in Malawian Children.

PLos One, 2010 Sep 7;5(9):e12589


Phiri KS, Calis JC, Siyasiya A, Bates I, Brabin B, van Hensbroek MB. New cut-off values for ferritin and soluble transferrin receptor for the assessment of iron defi-ciency in children in a high infection pressure area.

J Clin Pathol. 2009 Dec;62(12):1103-6.


Phiri KS, Calis JC, Kachala D, Borgstein E, Waluza J, Bates I, Brabin B, van Hensbroek MB. Improved method for assessing iron stores in the bone marrow.

J Clin Pathol. 2009 Aug;62(8):685-9.


Calis JC, Phiri KS, Faragher EB, Brabin BJ, Bates I, Cuevas LE, de Haan RJ, Phiri AI, Malange P, Khoka M, Hulshof PJ, van Lieshout L, Beld MG, Teo YY, Rockett KA, Richardson A, Kwiat-kowski DP, Molyneux ME, van Hensbroek MB. Severe Anaemia in Malawian Children.

N Engl J Med. 2008 Feb 28;358(9):888-99.


Calis JC, van Hensbroek MB, de Haan RJ, Moons P, Brabin BJ, Bates I. HIV-associated anemia in children: a systematic review from a global perspective.

AIDS. 2008 Jun 19;22(10):1099-112


JCJ Calis, HP Rotteveel, AC van der Kuyl, F Zorgdrager, D Kachala, M Boele van Hensbroek, M Cornelissen. Severe anaemia is not associated with HIV-1 env gene characteristics in Mala-wian children.

BMC Infectious Diseases 2008, 8:26


Phiri KS, Calis JC, Faragher B, Nkhoma E, Ng'oma K, Mangochi B, Molyneux ME, van Hens-broek MB. Long term outcome of severe anaemia in Malawian children.

PLoS ONE. 2008 Aug 6;3(8):e2903.


J Milledge, JCJ Calis, SM Graham, A Phiri, LK Wilson, D Soko, M Mbvwinji, AL Walsh, SR Rogerson, ME Molyneux, EM Molyneux; Etiology of neonatal sepsis in Blantyre, Malawi: 1996-2001;

Annals of Tropical Paediatrics; 2005 Jun; 25(2):101-10.


JCJ Calis, ML Bakker, RB Elens, M Borgdorff, AD Harries; Mortality in smear-negative Tubercu-losis patients in Phalombe;

Malawi Medical Journal; 2002; 14(2):13-14.


Books, or contributions to books


Aetiology, Pathogenesis & consequences of Severe Anaemia in Malawian Children. HIV and other factors. PhD Thesis; Amsterdam 2008; ISBN: 978-9022886-0


Chapters: Severe anaemia and Sickle cell disease in Protocols for the management of common childhood illnesses in Malawi; Blantyre 2004.