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Prof. dr. M.W. Hollmann MD PhD

Position
Professor
Main activities
Patient care, Research, Teaching
Specialisation
Anesthesiology, Intensive Care, Emergency Medicine, Pain Therapy and Palliative Care
Focus of research

Mission statement and ambition

Research in the Department of Anesthesiology will serve our patients by improving the safety and quality of perioperative care and (chronic) pain management. We will provide a stimulating and attractive academic environment for translational anaesthesia research of the highest quality, by providing a unique combination of top facilities for both clinical research and basic laboratory research. Continual motivation and inspiration of young researchers is the key driver in achieving our goals.

Research in the Department of Anesthesiology will focus on five main research lines, which are cardioprotection, anesthetics & signal transduction, (chronic) pain management, hemodynamic optimization and cerebral air embolism.

Cardioprotection is aligned with AMC’s main focus on cardiovascular diseases, and within our research, we set up several translational studies aiming to verify the in vitro and in vivo observed cardioprotective actions of pre- and postconditioning in humans by different pharmacological interventions and also remote ischemic conditioning.

Anesthetics and signal transduction belongs to AMC’s emphasis on inflammation and immunology. Within this area, we will further study basic mechanisms of (local) anesthetics, in particular the effects of (local) anesthetics on tumor biology.

Research related to (chronic) pain management addresses effects of regional anesthesia in diabetes pre-clinically as well as clinically. In addition, within the area of chronic pain management we focus on neuromodulation for neuropathic pain management. Research in neuromodulation will focus on two research lines; New stimulation modes and strategies for Dorsal Column Stimulation (DCS) and Neurostimulation for peripheral neuropathic pain (related to painful polyneuropathies or postsurgical pain).

Perioperative and hemodynamic optimization aims at improving the patients conditions perioperatively in close collaboration with the different surgical, nursing and paramedical specialties with a special focus on fluid- and hemodynamic management in all phases.

Within the area of cerebral air embolism research we set out to determine pathophysiological mechanisms, prophylactic and therapeutic treatments of patients suffering from cerebral air embolism.

Experimental Anesthesiology

Experimental Anesthesiology is imbedded in the common L.E.I.C.A. laboratory (Laboratory of Experimental Intensive Care & Anesthesiology). Worth mentioning, uniting the basic research efforts of the two sister disciplines in one lab, L.E.I.C.A is the first laboratory of its kind in the Netherlands and almost unique in Europe. Main research lines and good examples for translational science are cardioprotection and molecular mechanisms of anesthetics. Innovative growing ventures relate to the patho-physiology and therapy of cerebral air embolism, the quantification of mitochondrial oxygen in situ and the effects of anesthetics on tumor biology.

Research topic: Cardiovascular

L.E.I.C.A.’s cardioprotection group consists of several leading experts (Prof. Hollmann, Prof. Schlack, Prof. Preckel, Dr. Hauck-Weber and Dr. Zuurbier) in the field of Ischemia/Reperfusion (I/R) injury, pre- and postconditioning of the heart. Our research group within the LEICA laboratory focusses primarily on protection of heart, blood vessels and other organs against temporary periods of no oxygen. No oxygen conditions occur frequently in several clinical conditions (surgery, cardiac by-pass operations, shock, stroke, transplantation) and during an acute heart attack. This research therefore has a high clinical and society relevance: it can lead to improvements in clinical care and in the fight against cardiovascular diseases (still number one diseases in humans). 

We use cell culture models, isolated organs and in vivo models together with a large arsenal of different biochemical analysis methods (eg Western blotting, Real time PCR, immunohistochemistry, Elisa, FACS). Parts of the research are also performed in the clinical setting on the OR depending on the translational approach of the study. Main research focus is on 1) improving our understanding of the working mechanisms of protection, and 2) development of clinical relevant applications of protective strategies (i.e. translational research). 

1)         Unravelling the molecular machinery resulting in protection against cell death.

Our current focus is on mitochondrial function, caveolin signalling, mitochondria-hexokinase interactions and inflammatory mediators, because our research strongly indicates that these entities are the important nodal regulatory points of protective signalling

2)         Development of clinically-relevant protective strategies

Many basic laboratory derived protective strategies have ultimately failed clinically. An important reason for this failing has been that the basic researcher does not take fully into account the clinical conditions of the patients. Our laboratory is ideally suited to resolve these problems, because it constitutes the close collaboration between basic researchers and clinicians. In the protective strategies that we are developing and studying special care is therefore given to these clinical conditions, such as health status of the organisms, anaesthetics used, the presence of medical drugs. At this moment the focus is on remote ischemic preconditioning, special anaesthetic agents and helium breathing as clinically-applicable interventions to protect the organ/tissue against period of no oxygen.

Pathophysiology and therapy of cerebral air embolism

In a collaboration project with the Dutch Ministry of Defence, cerebral arterial gas embolism (a complication occurring in diving, but also as a complication of invasive medical procedures) is simulated in an experimental animal model and the pathophysiology, prevention and treatment is investigated.

 

Research topic: Molecular Mechanisms of anesthetics

GPCRs and Signaltransduction

Prof. Hollmann is a well renowned expert for the effects of anesthetics, in particular local anesthetics (LA), on signal transduction of G-protein coupled receptors (GPCRs). His findings on LA interactions with specific G protein subunits and G protein signalling transduction pathways have led to a revival of the systemic use of LA in the perioperative setting for indications others than pain management (e.g, modulation of inflammation and coagulation). Future research projects will try to identify the active parts within the LA structure, responsible for their protective effects as well as the corresponding target sites, allowing the development of new drugs: specifically indicated for modulation of coagulation and immune function, but lacking the typical “side effects” of LA, namely the sodium channel blockade. Dr. Stevens complements this research line with his experimental studies on neurotoxicity of anesthetics (mainly local anesthetics).

The basic research of Dr. Philipp Lirk is carried out in close collaboration with Departments of Neuroanatomy, Neurology, Cardiology and Clinical Neurophysiology. On the level of Basic science, we seek to explore the pharmacology and toxicology of local anesthetics. In past years, this research has focused on mechanisms and prevention of local anesthetic-induced neurotoxicity, while recently we have investigated epigenetic consequences of administration of local anesthetics and opioids. On the translational level, we are interested how diabetic neuropathy affects performance and safety of regional anesthesia, in particular peripheral nerve blockade. We have established a comprehensive model of regional anesthesia in diabetic neuropathy, including genetically altered rats (ZDF), neurophysiology to assess severity of neuropathy, percutaneous nerve blocks, behavioral testing, neurohistopathology and patch clamp electrophysiology.

 

Anesthetics & Tumorbiology

Shifting the focus of anesthesia research from the primarily perioperative outcome to long-term outcome, we set up a new, innovative research line investigating the effect of different anesthetic regimes on cell biology of tumor cells. Cancer surgery is usually performed under general anaesthesia with postoperative analgesia provided by opioids. The stress of surgery, general anesthetics, and opioid analgesics might be each immunosuppressive and specifically inhibit cell-mediated immunity that is critical for control of minimal residual disease. Opioids and stress also might increase angiogenesis, which promotes growth of cancer cells. Regional anaesthesia and analgesia may reduce the risk of cancer recurrence because it blocks the neuroendocrine stress response, decreases or eliminates the need for general anesthetics, and obviates the need for postoperative opioids

Clinical Research

Clinical research in the Department of Anesthesiology is rather diverse reflecting the diversity of the speciality. However, one of the main research lines pertains to cardioprotection. Since this is also in the spotlight of our experimental research, translation from the bench to the bedside is a strength of our research group.

Research topic: Cardiovascular

Cardioprotection: Anesthetics and Noble Gases

Combining the expertise of Prof Hollmann, Prof. Preckel, Prof. Schlack, Dr. Hauck, and Dr. Zuurbier makes for one of the world’s leading research groups in this field. Cardioprotective effects of various anesthetic strategies, tested on the cellular level and in animal experiments in L.E.I.C.A, were and will be tested in the clinical setting. In collaboration with the Department of Neurology from the AMC and the Tergooi-Ziekenhuis Blaricum we start to evaluate the application of helium in patients with acute stroke. Whether helium might also be used after cardiac arrest has been evaluated in collaboration with the Department of Intensive Care of the AMC. In addition, the department of Anesthesiology of the AMC is the leading center for an international multicenter study on the effects of ischemic remote pre- and postconditioning on outcome after coronary artery surgery. In this patient population, molecular mechanisms of remote preconditioning will be determined, allowing translation of basic findings from animal experiments (which are performed in close collaboration with the Department of Anesthesiology, University of Düsseldorf, Germany) to the clinical situation. We also intent to investigate (in collaboration with the Department of Surgery and the Department of Medical Medicine) the phenomenon of remote postconditioning in patients subjected to kidney transplantation.

Cerebral Perfusion

The main aim of this research line is to unravel the effect of oxygen on brain blood flow regulation. The results have important implications for amongst others the understanding and treatment of acute mountain sickness. For the treatment of patients with compromised cerebral autoregulation (CA) and oxygenation of the brain our studies evaluate(d) the importance of arterial hypertension and/or oxygen administration. Employing Near Infra-Red Spectroscopy (NIRS) to monitor cerebral tissue oxygenation, the role of oxygen in CA during changes in blood pressure or different levels of arterial blood oxygenation will be determined. Using new self-developed analyzing techniques, we are able to quantify the ability of the cerebral vasculature to counter-regulate sudden changes in blood pressure, which occur frequently in the perioperative setting. Vasoactive medication, intravenous and volatile anesthetics influence autonomic nervous system activity and thereby result in considerable changes in blood pressure and alteration of the systemic cardiovascular regulation. Cerebral vessels are richly innervated by sympathetic nerves, but there is controversy about the role of sympathetic nerves in regulation of cerebral blood flow (CBF). Leaving all other regulatory mechanisms of CA unaffected we will create a cerebral sympathetic activity “knock out” model by blocking the superior stellate ganglion (SGB) unilaterally to quantify sympathetic contribution to the regulation of cerebral blood flow (CBF). If  the regulation of the cerebral blood flow deteriorates during sympathetic blockade, the brain could be extra vulnerable to a decrease in blood pressure during the use of anesthetics.    

In addition, we will investigate the influence of changes in cerebral perfusion pressure and cerebral blood flow on CA in patients undergoing cardiac surgery with extracorporeal bypass, with and without pre-existing diseases (such as diabetes, stroke etc.).

Hemodynamic Optimization

Perioperative fluid and cardiovascular management is a core competence of the anaesthetists domain. Haemodynamic control has been linked to anaesthetic benchmarks, like nausea and vomiting, pain, tissue oxygenation, cardiopulmonary disorders, need of revision surgery, duration of hospital stay, and bowel recovery time. However, perioperative fluid handling comprises the type of fluids in general, their exact composition and the respective amounts. There is, for example, increasing evidence that an exclusive use of crystalloids might overload the interstitial space, leading to tissue edema, anastomotic leakage and thereby hampering patient outcome. Recent studies indicate perioperative fluid “restriction” during major surgical interventions to be beneficial to the patient. In different patient populations undergoing major abdominal surgery, we seek to determine optimal perioperative fluid management to reduce morbidity and improve gastrointestinal function, thereby reducing hospital stay and health care costs throughout the perioperative process. For this topic we work together with international experts and more than ten national partner anaesthesia departments

Decision Support

Clinical guidelines are a commonly used means to translate scientific evidence to good clinical care. They serve many purposes, but are generally agreed on to get the right (state of art) treatment for the right patient, reduce uncontrolled variation and improve clinical care. However, in practice, the implementation of guidelines may be limited by poor adherence. The aim of this project is to investigate which factors that influence non adherence to guidelines can be addressed by using decision support as an integrated system within the Patient Data Management System.  This system constantly monitors the data in the patient record and provides assistance in recognizing or even predicting potential complications and impending non-adherence and provide decision assistance with as little disruption of clinical care as possible. We proved that these systems can improve guideline adherence and can improve quality of care for a specific guideline and now aim to expand the system and optimize it to be more robust and applicable to other guidelines and situations. Moreover, we will look in to unintended consequences of decision support and reasons for residual non-adherence.

Optimizing perioperative metabolism as a cardiovascular risk factor

The incidence of diabetes mellitus in the general population is increasing into epidemic proportions and this is reflected in the surgical population. Patients with diabetes mellitus are at risk of postoperative complications; however the evidence for treatment of perioperative diabetes is surprisingly scarce. In this clinical research line we aim to identify risk factors for postoperative complications in patients with diabetes, improve detection of hyperglycaemia with novel monitoring techniques and study perioperative treatment in randomized clinical trials. Another focus is perioperative hyperglycaemia without a pre-existing diagnosis of diabetes mellitus and the diagnosis and impact of diabetes mellitus on perioperative cerebral and cardiac autonomic failure.

Research topic: other clinical research

Long-term effects of Regional anesthesia:

This research line has two objectives: The first is to define the impact of regional anesthesia (in particular ultrasound-guided peripheral nerve blockade) on functional outcome in orthopaedic surgery and development of chronic pain in a mixed surgical population. Secondly, to determine the effects of an opioid-sparing anesthetic regimen on tumor recurrence in patients undergoing breast cancer surgery. This project will be done in close collaboration with the German (Prof. H. Walczak) and Dutch (Dr. J. Jonkers) Cancer Center and translates our basic science findings on anesthetics and tumor biology to the clinical environment.

Other research efforts (Dr. P. Lirk) include the relevance of peripheral nerve block in the Emergency room focusing on hip fractures in the elderly (DEPTHIP study), the role of regional anesthesia in the prevention of phantom limb pain (PLATA study series), and safety aspects of Epidural anesthesia, a project carried out on behalf of the Dutch Society of Anesthesia.

 Big data and Patient Monitoring using Novel techniques

The future of science lies in the collaboration between research groups and departments. The Department of Anesthesiology at the AMC is one of only two non-US divisions to join the Multicenter Perioperative Outcomes Group (MPOG) project (Dr. P. Lirk, Drs. F. Kooij), and we have uploaded our first data the „Anesthesia Quality Institute (AQI)“ of the American Society of Anesthesiology (ASA). We are actively contributing towards MPOG research projects, and have submitted our first Research Proposal to the MPOG Research Committee.

In addition, we are exploring the potential clinical benefits of wireless monitoring of surgical (high-risk) patients using a new RFID technology (Sensium®), and have recently been awarded an Innovative Patient Care project grant to investigate how this monitoring might influence the “failure to detect” of the surgical patient, and thereby improve patient outcome.

 

Perioperative optimization

Enhanced Recovery After Surgery (ERAS) has proven to reduce surgical complication rates. We perform (implementation) research in close cooperation with GI surgeons, cardiothoracic surgeons, cardiologists, ICU physicians, dieticians, physiotherapists, nurses and ICT experts to improve current care practices (eating, drinking, mobilization and pain management), improve compliance and empower the perioperative patient (eHealth solutions). We also work together with machine and deep learning experts to develop real-time and continuous warning systems to signal and treat complications early.

Neuromodulation for chronic neuropathic pain treatment

The research line “New stimulation modes and strategies for Dorsal Column Stimulation” addresses the dose-response relationship between the amount of energy applied to the spinal cord and clinical outcome. This in relation to other variables, such as the anatomical target of stimulation and electrode configuration. Examples are the multi-center High And LOw energyfrequency RCT (HALO study) and the multicenter PAResthesia Vs Anatomical guided stimulation RCT (PARVA study).  The research line “Neurostimulation for peripheral neuropathic pain” is dedicated to gathering evidence for specific neuropathic pain diagnosis and to optimize clinical outcome in neurostimulation for this condition. Examples of investigator-initiated studies are Dorsal Root Ganglion (DRG) stimulation for neuropathic arm pain (AMS-01 study) and for phantom limb pain (AMS-02 study). In addition, we participate in several multi-center sponsored post-market studies An , for example: RCT foot pain (12-SMI-2014), RCT groin pain (24-SMI-2014), low back pain (18-SMI-2013) and post-surgical pain (20-SMI-2013).

Sedation - Safety &  Effectivity

 

In cooperation of the Departments of Anesthesiology and the Departments of Gastroenterology & Hepatology of the Academic Medical Center, Amsterdam and the University Medical Center Utrecht, this research line focusses on the differences in patient safety and satisfaction, effectivity and experiences by the gastroenterologists when sedation is given by a sedation anesthesia nurse (SAN), experienced in airway management, or a gastroenterologist, who is doing the gastroenterological procedure at the same time.

Prehospital Advanced Life Support – Airway management and ventilation during out-of-hospital resuscitation

In cooperation with the AmsteRdam REsuscitation STudies (ARREST) group of dr. Ruud Koster (AMC), this research focusses on airway management, ventilation and wave-form capnography during prehospital resuscitation. Main questions are: is endotracheal intubation or a supraglottic airway device the best airway management technique during Advanced Life Support? What ventilation frequency is optimal? What does the level of end-tidal CO2 tell us about the cause of the arrest? 

Research Networks & Large Clinical Trials

The Department of Anesthesiology is not only involved but also quite frequently main coordinating center for numerous national and international large-scale multi-center trials. The Department is/was frequently funded by the European Society of Anesthesiologists (ESA) Clinical Network (ESA-CTN, e.g. EUSOS, POPULAR (Steering committee), PROVHILO (Main center), LAS VEGAS (Main center), PROBESE (National coordinator), PROTHOR (National coordinator), PLATA (Main center), NECTARINE). Prof. Hollmann and Dr. Hemmes are part of the Protective Ventilation Network (PROVENET). Prof. Hollmann and Dr. Lirk together with Dr. Ridderikhof (Emergency Department AMC) are the leading investigators for the ZONMW supported PANAMA and DEPTHHip trial. Prof. Hollmann, Prof. Boer (VuMC) and Prof. Buhre (MUMC) are the Principal Investigators for the ZonMW funded national TRACE trial. Together with Prof. Boermeester (Department of Surgery, AMC) Prof. Hollmann is the lead investigator for the ZonMw supported EPOCH study. In addition, Prof. Hollmann is part of the NIMIT consortium (STW funded) and national coordinator for the EU COST project on anesthesia & cancer.

Collaborations – research environment

Nationally

The Anesthesiology Research Department has extensive and varying national collaborations within the AMC, but also with other Dutch academic centers. Among others the most intensive and prominent are:

Within the AMC we have established collaborations with the Dept. of Intensive Care (Prof. Vroom & Prof. M. Schultz), Dept. of Experimental Surgery (Prof. T.M. v Gulik), Dept. of Surgery (Prof. W.A. Bemelman & Dr. M. Idu & Dr. M.A. Boermeester), Dept. of Experimental Internal Medicine (Prof. T van de Poll), Dept. of Endocrinology (Prof. E. Fliers), Dept. of Gatroenterology & Hepatology (Prof. G.E.E. Boeckxstaens), Dept. of traumatology (Dr. C. Goslings), Dept. of Neurology (Dr. Y. Roos), Dept. of Cardiology (Dr. R. Koster – ARREST group)  and Dept. of Epidemiology (Dr. J.C. Korevaar)

Externally with the:

-          Department of Anesthesiology, Erasmus MC Rotterdam for research on Xenon & cardioprotection (Dr. J. Hofland)

-          Department of Anesthesiology, LUMC for research on preoperative assessment, pain and GPCR signal transduction (Prof. A. Dahan & Dr. A de Roode & Dr. F . Boer)

-          Department of Anesthesiology, UMCU for research on Sedation for Non-Anesthesiologists (Prof. C.J. Kalkman & Prof. H. Knape)

-          Department of Anesthesiology, OLVG for Decision Support research (Dr. J.E. Kal & Drs T. Klok)

-          Department of Anesthesiology, Diakonessen Hospital Utrecht for research on Neuromodulation (Dr. X. Zuidema)

-          Department of Neurology, Tergooi-Ziekenhuis Blaricum for Helium in stroke patients (dr. J. de Kruijk)

-          Department of Anesthesiology, AVL Amsterdam for research on pain therapy (Dr. M. Sramek)

-          Division of Molecular Biology, Netherlands Cancer Institute for research on anesthetics and tumor biology (Dr. J. Jonkers)

-          Ministry of Defense, Royal Netherlands Navy, and specifically the Diving Medical Center, Den Helder for research on cerebral arterial gas embolism.

-          ‘Dutch GDT & Benchmark network’; Departments of Anesthesiology of Westfriesgasthuis, Onze Lieve Vrouwe Gasthuis, VUmc, LUMC, Albert Schweitzer Ziekenhuis, Reinier de Graaf ziekenhuis, UMCG, Anthoni van Leeuwenhoek, Medisch Centrum Alkmaar

-          Prof. dr. J. Hans DeVries, Department of Endocrinology, AMC

-          Dr. J. Hermanides, Department of Anesthesiology, Pain and Palliative Care, RadboudUMC, Nijmegen

-          Department of Anesthesiology, Rijnstate Hospital Arnhem for research on Neuromodulation (dr. J.W. Kallewaard)

-          Department of Anesthesiology, Alrijne Hospital Leiderdorp for research on Neuromodulation (dr. K. Burger)

-          Department of Anesthesiology, Noordwest Hospital Group Alkmaar for research on Neuromodulation (dr. S. de Graaf and dr. H. Pelleboer)

 

Internationally

-          Department of Anesthesiology, Univ. of Heidelberg, Germany for research on local anesthetics, ultrasound-guided regional anesthesia and lung physiology (Dr. S. Herroeder & Dr. J Kessler)

-          Department of Anesthesiology, Univ. of Duesseldorf, Germany, Basic mechanisms of remote pre- and post-conditioning (Prof. Dr. I. Bauer, Prof. dr. B. Pannen)

-          Department of Anesthesiology, Univ. of Virginia, US for research on local anesthetics & GPCRs (Prof. M.E. Durieux)

-          Department of Anesthesiology, Perioperative and Critical Care Medicine, Parcelsus Medical University, Austria, for research on local anesthetics & pain therapy (Prof. Dr. P. Gerner)

-          Department of Anatomy, Innsbruck Medical University, Austria, for research on neurotoxicity of local anesthetics (Prof. Dr. Lars Klimaschewski)

-          Department of Gynecology, Laboratory of Clinical Biochemistry, Innsbruck Medical University, Austria, for research on epigenetic effects of local anesthetics

-          Department of Anesthesiology, Hospital for Joint Diseases, New York University, NY, for research on clinical application of peripheral nerve blocks (Prof. Dr. Arthur Atchabahian)

-          Department of Anesthesiology, University of Vienna, Austria for research on regional anesthesia & Outcome (Prof. P. Marhofer & Dr. E. Fleischmann)

-          Department of Anesthesiology, University of California, San Diego,VA San Diego Healthcare System, VS (Professor Hemal H. Patel, PhD Professor & Vice-Chair for Research,Director, UCSD Cardiac/Neuro Protection Laboratories

-          Klinik für Anästhesiologie und Operative Intensivmedizin Campus Kiel, Germany, (Prof. Dr. Martin Albrecht)

-          Department of Anesthesiology & Institute of Biochemistry and Molecular Cell Biology, RWTH Aachen University, Germany (Priv.-Doz. Dr. med. Christian Stoppe)

-     Universitätsklinik für Anästhesiologie und Schmerztherapie, Inselspital, 3010 Bern, Switzerland (Priv.-Doz. Dr. med. Jürgen Knapp, DESA EDIC)

-          Department of Critical Care, University of Pittsburgh for research on mean systemic filling pressure (Prof. M. Pinsky)

-          Department of Anesthesiology, Critical Care and Physiology, McGill University, Montreal, Canada (Prof. S. Magder)

-          Department of Anesthesiology and Critical Care, Hôspital Universitaire de Genève, Geneva, Switzerland (Dr K. Bendjelid & Dr B. Bollen)

-          Department of Anesthesiology, Rutgers University, New Jersey, USA (Prof. G. Atlas)

-          Department of Anesthesiology, Critical Care and Pain Medicine, "Sapienza" University of Rome, Rome, Italy, (Dr. F. Bilotta)

  

Funding
The work is funded by the German Research Society (DFG), NWO, Society of Cardiovascular Anesthesia (SCA), European Society of Anesthesiologists (ESA), Dutch Ministry of Defense, Medical Faculty of the University of Heidelberg, Germany, Medical Faculty of the Academic Medical Center (AMC), Amsterdam, The Netherlands and Institutional grants.

Key publications
  • Biere SSAY, van Berge Henegouwen MI, Maas KW, Bonavina L, Rosman C, Garcia JR, Gisbertz SS, Klinkenbijl JHG, Hollmann MW, de Lange ESM, Bonjer HJ, van der Peet DL, Cuesta MA, Minimally invasive versus open oesophagectomy for patients with oesophageal cancer: a multicentre, open-label, randomised controlled trial. LANCET 2012;379 (9829):1887-1892 [PubMed]
  • Pearse RM, Moreno RP, Bauer P, Pelosi P, Metnitz P, Spies C, Vallet B, Vincent JL, Hoeft A, Rhodes A [Contributors: Bauer P, Hoeft A, Metnitz P, Moreno R, Pearse R, Pelosi P, Rhodes A, Spies C, Vallet B, Vincent JL, Damster S, Golder K, Hewson R, Januszewska M, Leva B, Ramos V, Hoste E, Huyghens L, Jacobs R, van Mossevelde V, Opdenacker G, Poelaert J, Spapen H, Leleu K, Rijckaert D, de Decker K, Foubert L, de Neve N, Biston P, Piagnerelli M, Collin V, Blauwen Nd, Clauwaert C, de Crop L, Verbeke A, Roeselare HH, Derumeaux P, Gardin C, Kindt S, Louage S, Verhamme B, Druwé P, Lahaye I, Rosseel F, Rutsaert R, Vanlinthout L, de Kock M, Forget P, Georges P, Grosu I, Kahn D, Lois F, Momeni M, Pospiech A, Yemnga B, Jadoul JL, Malbrain M, Bosinceanu D, Collard E, Jorens P, Reyntiens D, Smitz C, Vercauteren M, Fagnoul D, van Obbergh L, Goranović T, Mazul-Sunko B, Toplice K, Oremuš K, Bešlić G, Duzel V, Hauptman A, Peremin S, Šribar A, Župčić M, Brod S, Mirković I, Bauer ZŠ, Belavić M, Blažanin B, Katušin ML, Krijan AB, Mišković P, Šimić-Korać N, Topić J, Žilić A, Žuni J, Acan I, Adanić M, Ivanov N, Šarić JP, Tomulić K, Visković N, Bošnjak S, Drenjančevic IH, Kristek G, Kvolik S, Markić S, Rakipovic AS, Tot OK, Venzera- Azenic D, Fabris LK, Bačak-Kocman I, Balenović I, Bandić D, Deutsch PAJ, Divjak L, Filipović IG, Gužvinec Z, Krznarić Z, Lončarić Y, Magaš JV, Mitrović M, Okić M, Pavlek M, Ramov E, Rezek K, Sekulić A, Tomasevic B, Mirić M, Tomašević A, Mahečić TT, Vrbanović V, Bobinac M, Božić A, Debelic D, Frkovic V, Batinica IM, Baranović S, Gavranović Ž, Kikec M, Maldini B, Marić S, Agnić I, Delić N, Dropulić N, Gašpić TK, Ilić D, Ivančev B, Karanović N, Kuščević D, Marović Z, Milić M, Nevešćanin A, Petković T, Smoje M, Brozović G, Jelisavac M, Matolić M, Oberhofer D, Pavičić AM, Šakić K, Bozovic MD, Krecek ZK, Krobot R, Andabaka T, Bratanić M, Dzepina O, Kraljev M, Šeric J, Šimurina T, Grujić R, Nacevski-Bulaja B, Barižon M, Danira V, Dražen B, Dušanka K, Halužan MB, Joško Ž, Katica R, Labor M, Marinković TG, Mihovilčević D, Marija B, Srečko M, Vranković S, Kyprianou T, Neophytou K, Cerny V, Cvachovec K, Belikova B, Drab M, Hudacek K, Krikava I, Stourac P, Zadrazilova K, Bicek V, Brabcová M, Klozová R, Vajter J, Vymazal T, Toft P, Blichfeldt L, Hansen BD, Moller K, Nielsen JS, Frederiksen J, Andersen JD, Kühne JP, Leivdal S, Stendell L, Simonsen M, Zoltowski MK, Ali ZS, Freundlich M, Pilypaite J, Clausen NG, Thorup L, Hansen F, Bestle M, Hansen CS, Afshari A, Bille AB, Lefort M, Secher EL, Liboriussen L, Herodes V, Härma E, Marvet K, Pool K, Kallas P, Mägi TK, Sütt J, Vijar K, Visk E, Vinnal M, Ellermaa J, Liibusk L, Tikkerberi A, Falk I, Mällo E, Talving J, Pettilä V, Hovilehto S, Kirsi A, Mustola S, Tiainen P, Toivonen J, Dabnell S, Kaminski T, Sysimetsa A, Kaukonen M, Silvasti P, Vainio K, Lund V, Sjövall S, Saarinen K, Viitanen M, Ahonen T, Alaspää A, Zittling R, Saarinen A, Moisander A, Wagner B, Laru-Sompa R, Elomaa E, Lavonen L, Nevantaus J, Geier K, Kavasmaa T, Koorits U, Kubjas M, Lauritsalo S, Ottelin L, Palve M, Pynnönen J, Rääbis I, Saarelainen M, Heikkilä T, Kontula T, Lehtimäki M, Liimatainen J, Moilanen-Oikarinen M, Pakarinen M, Palanne R, Seppänen H, Pulkkinen A, Vääräniemi H, Paananen S, Koskenkari J, Sälkiö S, Vakkala M, Koskue T, Loisa P, Laitio R, Hautamäki R, Koivisto SP, Futier E, Lefrant JY, Leon A, Bonnet F, Marret E, Spielvogel C, Papageorgiou C, Szymkiewicz O, Tounou-Akue F, Aubrun F, Bonnet A, Gazon M, Guiraud M, Laurent V, Béclère A, Tachon G, Demars N, Dumenil AS, Mercier F, Landais A, Mentec H, Bazin M, Gonnu S, Petit A, Albaladejo P, Almeras L, Bataillard A, Rossi-Blancher M, Lefrant JY, Barthel F, Hallel D, Sbai H, Khalifeh P, Lidzborski L, Jully M, Platon E, Pottecher J, Baumgarten R, Schultz C, ElMiloudi F, Lefebvre J, Waton K, Sprunck A, Steib A, Thibaud A, Thuet V, Kieffer V, Dubois-Vallaud D, Jacob L, Becanne X, Cherfaoui S, Gauzit R, Godier A, Lakhdari M, Samma C, Bigeon JY, Burtin P, Halchini C, Lacroix M, Pinna F, Barbes A, Just B, Mateu P, Benayoun L, Berger P, Granier N, Perrigault PF, Libert N, de Rudnicki S, Merat S, Bourdet B, Ferré F, Minville V, Piriou V, Rague P, Wallet F, Lebuffe G, Desbordes J, Robin E, Ichai C, Orban JC, Marx G, Sander M, Gottschalk A, Piontek A, Unterberg M, Hilpert J, Kees M, Triltsch A, Wiegand-Löhnert C, Glöckner C, Hohn A, Rose E, Schröder S, Wiese O, Awlakpui E, Scheidemann M, Wittmann M, Ramminger A, Dresden C, Gama de Abreu M, Heller A, Marx C, Neidel J, Goldmann A, von Heymann C, Laetsch B, Maahs E, Scholz L, Frenzel D, Massarat K, Lenhart FP, Reichle F, Rudlof K, Borchers F, Buettner C, Schmutzler M, Burgard G, Lucht A, Wagner J, Pilge S, Schneider G, Untergehrer G, Bis B, Krassler J, Dittmann J, Haberkorn J, Eberitsch J, Eberitsch K, Nippraschk T, Wepler U, Engelen WC, Nau C, Scholler A, Schüttler J, Wintzheimer S, Bloos F, Braune A, Fergen D, Ludewig K, Paxian M, Reinhart K, Graf N, Schwarzkopf K, Berger K, Habicher M, Kasperiunaite R, Savelsberg S, Krep H, Reindl M, Weber M, Bauer W, Bingold F, Christ S, Friederich P, Kaviani R, Auer P, Bonnländer G, Drescher J, Braun R, Eichenauer T, Kerner J, Bierbaum K, Brünner H, Grond S, Perez-Platz U, Andresen B, Linstedt U, Stegmann N, Erkens U, Kopcke J, Meyer A, Brestrich H, Ernst S, Merkel S, Krieger L, Luers F, Weyland A, Noeldge-Schomburg G, Menckie T, Wasmund C, Bredtmann RD, Erler I, Raufhake C, Haumann C, Möllemann A, Oehmichen U, Sergejewa O, Lehning B, Czeslick E, Geyer M, Malcharek M, Sablotzki A, Stier M, Feld F, Rossaint R, Simon V, Armaganidis A, Koulenti D, Kotanidou A, Nanas S, Papastylianou A, Psevdi A, Stathopoulos A, Voulas A, Kanna E, Koutsikou A, Moustaka A, Chovas A, Komnos A, Zafiridis T, Franses J, Lavrentieva A, Koraki E, Katsenos C, Kasianidou MF, Nasopoulou P, Spyropoulou E, Gousia C, Katsanoulas C, Lathyris D, Kyriazopoulos G, Sfyras D, Tsirogianni A, Kostopanagiotou G, Lignos M, Matsota P, Christopoulos C, Mouratidou A, Vrettou E, Boufidis S, Moka E, Arnaoutoglou E, Koulouras V, Nakos G, Papathanakos G, Anthopoulos G, Choutas G, Karapanos D, Tzani V, Gkiokas G, Nastos K, Nikolakopoulos F, Dragoumanis C, Nikitidis N, Pneumatikos I, Theodorou V, Zacharouli D, Kandi S, Tasopoulos K, Arvaniti K, Matamis D, Mplougoura E, Petropoulou P, Soumpasis I, Amaniti E, Giannakou-Peftoulidou M, Gkeka E, Soultati I, Kokinou M, Papatheodorou L, Stafylaraki M, Giasnetsova T, Gritsi-Gerogianni N, Kydona C, Kiskira O, Koulentis I, Apsokardos A, Dimitropoulos K, Soldatou O, Nathanail C, Papazotos A, Tsakas P, Clouva-Molyvdas PM, Kolotoura A, Sartzi M, Papanikolaou S, Polakis P, Karatzas S, Kyparissi A, Papavasilopoulou T, Koukoubani T, Mastora E, Spyropoulou-Pagdatoglou K, Nyktari V, Malliotakis P, Papaioannou A, Bekos V, Maragkou E, Spring A, Evagelatos S, Ioakeimidou A, Noulas N, Molnár Z, Csüllög E, Elekes E, Molnár T, Katona Z, Kremer I, Miko A, Csomos A, Galambos Z, Szucs A, Nyikos G, Szekeres G, Szabo E, Kranitz K, Simon M, Szigeti J, Gaál E, Havas A, Ille A, Bráz K, Nagy G, Sigurdsson G, Sigurbjörnsson FT, Sigurdsson GH, Kárason S, Sigurdardottir EE, Blöndal Á, Gunnarsson B, Westbrook A, Broderick A, Hafeez P, Hanumanthaiah D, Brohan J, O'Chroinin D, Bailey K, Ramamoorthy K, Doyle Y, Freir N, O'Rourke J, Jonson P, Saeed S, Hayes I, Loughrey J, Frohlich S, McCauley N, Ryan D, Fitzpatrick G, Kevin L, Thomas J, Warde B, Woolhead A, Duggan M, Egan C, Crowley S, Lebese S, Bergin A, Page R, Collins D, McKenny M, Della Rocca G, Grasso S, Bresciani A, Carmino L, Ghelfi S, Lorenzelli L, Novelli MT, Pescarmona C, Roasio A, Gatta A, Nastasi M, Sanseverino M, Tinti C, Bianchin A, Tormena M, Franco A, Marini F, Di Mauro P, Rapido F, Tommasino C, Bellotti F, Boninsegna D, Castellani G, Sances D, Spano G, Tredici S, Vezzoli D, Fucecchio I, Bacci A, Coppini R, Dell'unto S, Mori E, Stanzani MR, Tosi M, Collareta M, Forfori F, Franchi M, Mancino G, Battistella M, Baricocchi E, Bona F, Debernardi F, Giacoletto G, Iacobellis A, Massucco P, Moselli N, Muratore A, Palomba G, Sardo E, de Simone M, Suita L, Zocca E, Bucci B, Della Corte F, Piciucco T, Viarengo V, Bettelli G, Cantarini E, Giampieri M, Tanfani A, Recchia E, Milano SR, Bignami E, Bruno G, Costagliola R, Gandolfi A, Greco M, Lembo R, Monti G, Nicelli E, Pasculli N, Turi S, Baroselli A, Brazzoni M, Buttazzoni M, Buttera S, Centonze C, Serena G, Spagnesi L, Toretti I, Vilardi A, Zearo E, Arpino I, Baraldi S, Guarnerio C, Molene V, Monea MC, Vaccarisi E, Vicari L, Albante A, Aversano M, Loiacono C, Marandola M, Fusari M, Petrucci N, Galla A, Mascia A, Primieri P, Di Noto A, Gratarola A, Molin A, Spagnolo L, Spena C, Calligaro P, Marchiotto S, Merlini A, Pedrazzoli E, Perina G, Visentin R, Fumagalli R, Garbagnati A, Manetti B, Snaier C, Somaini M, Farnia A, Nani R, Pierantonio N, de Michele M, Gazzanelli S, Pugliese F, Ruberto F, Anna US, Bergamini E, Tassinati T, Capuzzo M, Cirillo V, Tufano R, Oggioni R, Parrini V, Brunori E, Capone M, Carbone L, Corradetti F, Elisei D, Fiorentino S, Francesconi M, Gattari D, Gorgoglione M, Lacobone E, Minnucci F, Montironi C, Riccioni G, Tappata G, Zompanti V, Verdenelli P, Cerutti E, Ranieri VM, Golubovska I, Grigorjevs S, Rikmane M, Rozkalne D, Stepanovs J, Suba O, Kazune S, Miscuk A, Nemme J, Oss P, Sipylaite J, Macas A, Ragaisis V, Kontrimaviciute E, Tomkute G, Boerma C, Kramer IF, Poeze M, Ziekenhuis A, Maria J, Pelzer G, Winsser L, Nijsten M, Schoorl M, Spanjersberg R, Buhre W, Dieleman S, van Klei W, Bouw M, Pickkers P, van der A M, Schreiner F, Zandvliet R, van den Berg R, de Wit E, Keijzer C, Hollmann M, Preckel B, van Acker G, Dennesen P, Veld B, Kuijpers-Visser A, Inan T, Koopman-van Gemert A, Ponssen H, Brouwer T, Koopmans M, van Bommel J, van Duijn D, van der Hoven B, Ormskerk P, Beck O, Schiere S, Reidinga A, Venema A, Hoogendoorn M, Olthof K, Flaatten H, Jammer I, Dokka V, Monsen SA, Ytrebo LM, Noursadeghi M, Shahzad A, Boksasp OD, Roiss C, Strietzel HF, Gina A, Berntsen S, Haugland H, Vingsnes SO, Axelsson P, Olsen T, Katre S, Aakeroey K, Mikstacki A, Tamowicz B, Bożiłow D, Goch R, Grabowski P, Kupisiak J, Małłek M, Szyca R, Kostyrka W, Choma R, Jankowski G, Kościelniak W, Pietraszek P, Szarowar B, Matos R, França C, Lacerda AP, Ormonde L, Rosa R, Pereira I, Vitor P, Bento HC, Lopes MR, Carvalho M, Faria M, de Sousa AC, de Freitas P, Almeida E, Mealha R, Vicente R, Monte R, Rua F, Barros N, Esteves F, Gouveia Pinheiro CM, Real V, Oliveira VM, Oliveira MF, Martins I, Saraiva JP, Assunção JP, Bártolo A, Carvalho A, Correia C, Martins S, Milheiro R, Diaz A, Gonçalves MI, Ribeiro R, Estilita J, Glória C, de Almeida J, Barros F, Ramos A, Camara M, Maul ER, Nobrega J, Langner A, Maia D, Afonso O, Faria F, Serra S, Botelho MM, Ferreira P, Mourão L, Oliveira AV, Resende M, Aleman M, Fonseca J, Isidoro M, de Meneses H, Pêgas A, Pereira J, Pereira L, Ramos B, Matos F, Castro MdLG, Martins A, Ramos C, de Sousa M, Bento L, Botas C, Lopes V, Mendes R, Grigoras I, Blaj M, Damian M, Lupusoru A, Ristescu I, Codreanu M, Diaconescu C, Nistor A, Stelian DS, Streanga L, Berneanu M, Bordeianu C, Florenta C, Iacob A, Lupu MN, Mocanu I, Moraru C, Meran C, Nicolae B, Sandu M, Turcanu R, Epure F, Grigore M, Hotaranu C, Popescu N, Baban O, Baciu M, Ciobanu A, Denciu CI, Gurau V, Maftei I, Moldovan I, Ungureanu L, Bogdan P, Corneci D, Dinu M, Madalina D, Rely M, Silvius N, Tomescu D, Gabriela D, Dinescu SA, Calin M, Ionescu D, Margarit S, Vasian H, Albu C, Balasa C, Cadrigati A, Dragulescu D, Gavra L, Hentia C, Macarie C, Manescu M, Nediglea I, Ocica D, Ovidiu B, Papurica M, Plavat C, Popa C, Ramneantu M, Sandesc D, Sandici Z, Sarandan M, Belciu I, Tincu E, Ursu I, Aignatoaie M, Huzuneanu M, Cocu S, Hagau N, Ciubotaru R, Copotoiu SM, Copotoiu R, Ioana G, Kovacs J, Leonard A, Szederjesi I, Genoveva V, Mosnegutu S, Surbatovic M, Djordjevic D, Djordjevic B, Grujic K, Jovanovic D, Krstic-Lecic I, Obradovic J, Zeba S, Jevdjic J, Miletic M, Zunic F, Bulasevic A, Brko R, Gazibegovic N, Kendrisic M, Vojinovic R, Firment J, Zahorec R, Capková J, Grochova M, Trenkler S, Griger M, Bakosova E, Kvasnica M, Saniova B, Sulaj M, Zacharovska A, Simkova A, Číková A, Gebhardtova A, Hanuljaková S, Koutun J, Martonová A, Žilinčárová V, Galkova K, Krbila S, Sobona V, Ocenasova M, Novak-Jankovic V, Stecher A, Stivan F, Grynyuk A, Damjanovska M, Kostadinov I, Knezevic M, Malivojevic M, Borovsak Z, Kamenik M, Mekiš D, Osojnik I, Kosec L, Kapš SO, Aleksic D, Gerjevič B, Kalan K, Ursic T, Aldecoa C, González JM, Artigas A, Garcia A, Lisi A, Perez I, Perez G, Poch N, Vaquer S, Balciscueta G, Barrasa H, Cabanes S, Maynar J, Poveda Y, Rodero AQ, Vallejo A, Duque P, Garcia-Bunger B, Elvira MA, Lajara AM, Palencia M, Ramos R, Fernandez AS, León JT, López JI, Murillo FY, Ramirez ET, Rico PU, Patricia M, Vizcaíno M, Bernat Álvarez MJ, Real KP, Serra AM, Aracil N, Bodega BM, García RF, García MÁ, Gordon BdlQ, Jodrá AG, López ADS, Ros JJL, Soto RA, Sepúlveda I, Díez EP, Fernández LF, Gulina CS, Arviza LP, Fernandez LM, Gómez AR, Martínez CA, Rodríguez ABR, Soto CL, Garcia C, Lorenzo M, Pinilla E, Rico J, Ruperez I, Alonso E, Leira F, Maseda DPE, Royo C, Villagran J, Candi GM, Esteva GE, Folgado RM, Fornaguera NJ, Montse PC, Prat AS, Sintes D, Arteta DA, Delgado HG, López-Cuervo JF, López MC, Ramírez A, Saldaña FJ, Aliste P, Anchuelo AH, Campos AG, Catalán M, Gómez MG, Gonzalaez OG, López EL, Navacerrada IR, de Quevedo SA, Serrano MG, Silvestre FPC, Torrente FM, Arocas B, Martinez EP, Soro M, Maroto F, Algarra RR, Aleixandre IS, Argente GR, Lleó AB, Rubio AV, Sánchez JLV, Valcárcel IE, Balust C, Balust J, Borrat X, Carretero MJ, Gracia I, Matute P, Mercadal J, Pujol R, Tena B, Ubre M, Albalad DD, Alcaide CM, Caballero J, Cervantes AC, Clanchet MdN, Estruch NM, Ferrer MB, Fornells AL, Galera ET, Martinez IG, Muñoz SM, Pelavski A, Perez PT, Posada MAG, de Prat IC, Rello J, Serrano LG, Sieiro JMN, Silva L, Sole MJC, Suñé AB, Villach IR, Herreras JIG, Poves R, Rafael BM, Almeida IM, Collates AF, Bartolomé MJ, Cimadevilla B, González AMG, Llevot JMR, Mira JCDdT, Molina BG, Pardo S, Sánchez CL, Williams M, Zaldibar E, Corsini LM, Fraile JRR, de la Lastra M, Sacramento MK, Saña FJL, Ålvarez JT, Bulnes MLC, Carrasco VG, Crespo MdRM, Cubillos DN, Laza EL, Pérez MdPH, Seisdedos ÁA, Torres BF, Ampuero MS, Llano MC, Mata E, Munoz M, Orts M, Planas A, Ramasco F, Roman C, Durán MV, Fernandez SdR, Otero YS, Pineiro SL, Pardal CB, Alcantud JF, Antolinos MA, Barrios F, Casanova AC, Castro MR, Crespo BI, Felipe UL, Fuster ML, García MS, Garrote BH, Gonzalez RM, Granero MJM, de la Guía CL, López RC, López SdF, Martinez JJM, Mostaza AG, Moreno AJ, Osado IR, Pastor AB, Peña R, Pérez MR, Piña MA, Romero CA, Rodríguez ER, Sáez VP, Safatle F, Salvan JH, Sampedro MG, de la Torre PA, Toro JP, Unzúe CL, Vargas MJ, Bernal DG, Echevarria M, Iglesias AU, Loza A, Morillo AR, Serrano PD, Sevilla FCB, Cacho E, Calderón R, Dufur M, Marginet C, Monedero P, Yepes MJ, Alvarez LR, Carballal FF, Castiñeiras AP, García PD, López LR, Maceiras PR, Puente MSM, Rilo MTR, Alonso AET, Fernández SR, García RO, García AP, Puentes RB, Aguado DN, Carballo CL, Fernandez RF, Presedo AT, de Rabago RBD, Velasco AR, Capel YJ, Cortés AF, García EM, Gimeno LM, Klamburg J, Omedas RC, Núñez MG, Maristany CL, Ruiz EM, Artigas X, Castrillón S, Espinosa N, Gomez-Caro AM, Illa S, India I, Martín-Huerta B, Moral V, Moreno M, Fernández CI, García VF, Hernández PP, Checa AAH, Diaz DS, Noguera ML, Varela IP, Gallego MG, García OM, Irujo JJA, Perrino CG, Picazo JR, Timoneda FL, Arroyo MM, Blanco IA, Borja MM, Burcio SM, Castro NM, Cerdeiriña AP, Concostrina MdlT, Cristina MV, Díaz TD, Esteruelas JA, Ingelmo II, Insuga PD, Llamas EC, Lopez JJM, Martín MB, Martín EE, Mesa EU, Monterde ML, Montoiro PA, Móstoles MLG, Olarte EV, Pérez AM, Perez FD, Romero AS, Rous DP, Ruiz NM, Ruiz JAP, Saiz AR, Terol AdlV, Toha AC, Utrera FA, Alberdi F, Elósegui I, García J, Garde PM, Mintegui EI, Sáez IG, Salas E, Zabarte M, Diaz-Boladeras RM, Mora-Guevara E, Zamora JF, Bonet A, Salo L, Salinas U, Zaballos J, Alvarez AA, Garrido CJ, Roa JRH, Vidal FG, Garcia-Egea J, Elson MZ, Seron-Arbeloa C, Asensio MAM, Simeón RG, Alameda LEM, Angulo GO, Aranzubia M, Arcas JJ, Arevalo J, Belvert BQ, Calvo CP, Cremades M, Crespo P, Cuarental A, del Olmo M, Fernández PT, Vega JLFGIG, Herrera EII, Llorente MAA, Rabes CM, de Maeyer AG, Marquez MP, Mendoza DL, Muñoz JMM, Martínez NA, Oviedo AS, Garrigues PB, Íñigo JA, Ferrandiz ST, Sanchez-Morcillo S, Sánchez ML, Parra AM, Vidal SG, Allué RM, Etayo BZ, Rodriguez RB, Villen LM, Jimenez CM, de Zayas RS, Moreno CD, Pacheco FS, Pascual JLA, Gude FT, Riestra EM, Delgado FC, Prados MVdlT, Barrios J, Cervera-Montes M, García-Sanz M, García V, Sanmiguel G, Álvaro JL, Barrachima BB, Bermejo FJR, Pilar AGF, Garcia M, Ramírez CN, Ramos JG, Samaniego LA, Belenguer-Muncharaz A, Ferrándiz-Selles A, Mateu-Campos ML, Domínguez D, Espinosa E, León T, Betancor NO, Cortes JG, Díaz JJD, Canalechevarria AM, Novales BF, Peña JMG, Delgado TR, Roquerio BS, Sainz JJG, Soto TT, Chew M, Seeman-Lodding H, Dahm P, Hergès HO, Lundborg C, Söndergaard S, Rylander C, Sari F, Tibblin AO, Adolfsson A, Klarin B, Schrey S, Merisson E, Rydén J, Divander MB, Hedin A, Hedlund D, Lindkvist MA, Jawad M, Layous L, Wernerman J, Björne H, Brattström O, Olheden S, Oldner A, Sellden E, Walder B, Wickboldt N, Rossi A, Steiner L, Djurdjevic M, Lussmann R, Geisen M, Hofer C, Turina M, Grocott M, Goldhill D, Everett L, Harris K, Wright M, Adams D, Alderson L, Baker J, Christie I, Ferguson C, Hill M, Holmes K, Hutton A, Minto G, Moor P, Porter A, Struthers R, Akotia N, Belhaj A, Chang S, Collantes E, Eigener K, Husband M, Khan A, Kong ML, McAlees EJ, MacDonald N, Niebrzegowska E, Parnell W, Smith A, Chhatwal A, Jhingan S, Muswell R, Poon Y, Singh N, Stephens R, Vasan R, Waife N, Weda T, Clarke A, Szakmany T, Fletcher S, Rosbergen M, Blunt M, Prince L, Wong K, Kumar R, Stilwell S, Couper K, Crooks N, Gao-Smith F, Melody T, Snaith C, Patel J, Parekh D, Yeung J, Loughnan B, Moosajee V, Rope T, Edger L, Dawson J, Hadfield D, Hopkins P, McDonald L, Willars C, Campbell G, Craig J, Smith A, Ladipo K, Lockwood G, Moreno J, Ballington R, Hamandishe S, Rogerson D, Cowman S, Hayden P, Pinto N, Sandhar T, Arawwawala D, Brotherston L, Mitchell-Inwang C, Walsh H, Alagarsamy F, Goon S, Karcheva S, Krepska A, McKinney B, Patil V, Batchelor N, Day C, Finch L, Gibson C, Grayling M, Hubble S, Key W, Knight T, Loosley A, Margetts P, Stewart H, Bewley J, Hurley K, Murphy R, Philpott C, Pollock K, Sweet K, Thomas M, Tucker K, Windsor D, Conway D, Gold S, Quraishi T, Cupitt J, Baddeley S, Brown JD, Foo I, Mantle D, Carvalho P, Huddart S, Kirk-Bayley J, Smith R, Milligan L, Poulose S, Sarkar S, Nolan J, Pedley E, Padkin A, Pesian S, Rajamanickam S, Ramkumar K, Thomas J, Crayford A, Turner A, Bottrill F, Webb S, Jhanji S, MacCallum N, Wessels K, Wigmore T, Meikle A, Wilson S, White S, Bonnett A, Rushton A, Williams C, Zuzan O, Hall A, Montgomery J, Piggot A, Read R, Stocker M, Tamm T, Agarwal B, Ward S, Brown L, Joy M, Venkatesh S, Hughes T, Zsisku L, Roy A, Hooper V, Mouland J, Nightingale J, Rose S, Chiam P, Chohan H, Dickson C, Gibb S, Higham C, Harvey C, Janarthanan C, Jones L, Kapoor A, Moll M, Roberts L, Saunders D, Arnold G, Gibbs C, Jhurgursing M, Pierro D, Pritchard F, Doyle P, Templeton M, Wilson R, Zantua K, Collyer T, Featherstone J, Worton R, Bruce J, McGuigan K, Price G, Moreton S, Pulletz M, Anderson H, Baxter I, Beckingsale A, Callaghan M, Datta A, Dawson J, Gollogly J, Izod C, Lobaz S, MacFie C, Patel M, Payne H, Singh R, Timms G, McLeod S, O'Brian P, Horner E, Joshi V, Stuart-Smith K, Seale T, Bolger C, Collins H, Ekins E, Hawkins L, Jonas M, Linford K, Wadams B, Beach M, Vizcaychipi M, Jewsbury W, Davies S, Balaji P, Kangaraj M, Pissay N, Smith N, Gopalakrishnan S, MacKinnon J, Strandvik G, Francis R, Jennings A, Keating M, Kumar S, Leese S, Magee C, Pilsbury J, Ralph J, Riddington D, Sachdeva R, Snelson C, Vasanth S, Wilde J, Lavender B, Lyons R, Watters M, Adams T, Dyer S, Tindall L, Claxton A, Netke M, Akouds E, Bates D, Gallagher H, Hatton J, Holroyd W, Mitra A, Nurse T, Reed D, Desikan S, Barber R, Childs S, O'Carroll-Kuehn B, Wyldbore M, Al-Abdaly A, Amatya S, Bhaskaran S, Chandan G, Chaudhry S, Chikungwa M, Earnshaw G, Grewal M, Haque S, Hawkins J, Javaid A, Jackson C, Kamel M, Marla R, Mculloch DA, Parker T, Salib Y, Saravanmuthu R, Secker C, Sockalingam S, Taylor A, Austine P, Kanade V, Paal D, Mok MUS, Burtenshaw A, Davis L, Ellahee P, Freeman D, Pierson R, Wollaston J, Karmarkar A, Ball C, Calton E, Maxwell L, Walker R, Bland M, Bullock L, Harrison-Briggs D, Hodge P, Krige A, Dempsey G, Hammell C, Loveridge R, Parker R, Snell J, Wright C, Baker A, Barr K, Belcher A, Bonnington S, Bougeard AM, Fitzgerald E, Ford R, Gillard C, Griffiths L, Greenberg L, Huber J, Mathieu S, Richardson N, Tompsett L, White N, Patel S, Corner V, Thomas R, Trodd D, Wilson J, Copley E, Flutter L, Hulme J, Susarla J, Thwaites A, Jayasundera S, McAfee S, Chantler J, McKechnie S, Neely J, Mouton R, Scarth E, Soar J, Buss J, Currie V, Sange M, Kuttler A, Power F, Alexander D, Dunne K, Shinner G, Black E, Haldane G, Kerr J, Saran T, Ward G, Jefferies F, Alexander P, Royle A, Nahla F, Bowles T, Gregory M, Ahern R, Cartlidge D, Craker L, Thompson C, Bidd H, Giles J, Manser A, Parry G, Chan P, Das D, Fahmy N, Higgins D, Khader A, Stone A, Leonardi S, Rose O, Bright E, Ercole A, Rafi MA, Ramasamy R, Sheshgiri B, Merrill C, Page V, Walker E, Harris S, Hughes S, Morrison A, Razouk K, Ayman M, al-Subaie N, Arif F, Cashman J, Cecconi M, Edsell M, Fossati N, Hammond SJ, Hamilton M, Lonsdale D, Moran C, Siegmueller C, Velzeboer F, Wong P, Jakeman A, Mowatt C]] , Mortality after surgery in Europe: a 7 day cohort study. LANCET 2012;380 (9847):1059-1065 [PubMed]
  • Vlug MS, Wind J, Hollmann MW, Ubbink DT, Cense HA, Engel AF, Gerhards MF, van Wagensveld BA, van der Zaag ES, van Geloven AAW, Sprangers MAG, Cuesta MA, Bemelman WA [Contributors: Preckel B, Bossuyt P, Gouma D, van Berge Henegouwen M, Fuhring J, van Lenthe IP, Bakker C, King-Kalimanis B, Hofland J, Dejong C, van Dam R, van der Peet D, van Zalingen E, Noordhuis A, de Jong D, Goei TH, de Stoppelaar E, van den Dongen M, van Tets W, van den Elsen M, Swart A, deWit L, Siepel M, Molly G, Juttmann J, Clevers W, Bieleman A, Coenen L, Bonekamp E, van Abeelen J, van Iterson-de Jong D, Krombeen M]] , Laparoscopy in combination with fast track multimodal management is the best perioperative strategy in patients undergoing colonic surgery: a randomized clinical trial (LAFA-study). ANN SURG 2011;254 (6):868-875 [PubMed]
  • Sierink JC, Treskes K, Edwards MJR, Beuker BJA, den Hartog D, Hohmann J, Dijkgraaf MGW, Luitse JSK, Beenen LFM , Hollmann MW, Goslings JC [Contributors: Saltzherr TP, Schep NWL, Streekstra GJ, van Lieshout EMM, Patka P, Klimek M, van Vugt R, Tromp TJN, Brink M, Harbers JS, El Moumni M, Wendt KW, Bingisser R, Ummenhofer W, Bless N]] , Immediate total-body CT scanning versus conventional imaging and selective CT scanning in patients with severe trauma (REACT-2): a randomised controlled trial. LANCET 2016;388 (10045):673-683 [PubMed]
  • Hemmes SNT, Gama de Abreu M, Pelosi P, Schultz MJ [Contributors: Hemmes SNT, Gama de Abreu M, Severgnini P, Hollmann MW, Binnekade JM, Wrigge H, Canet J, Hiesmayr M, Schmid W, Jaber S, Hedenstierna G, Putensen C, Pelosi P, Schultz MJ, Sessler DI, Lachmann B, Kacmarek RM, Slutsky AS, de Baerdemaeker L, de Hert S, Heyse B, van Limmen J, Mulier JP, Velghe D, Jamaer L, Vandenbrande J, Bugedo G, Florez J, Goranović T, Mazul-Sunko B, Bluth T, Güldner A, Kiss T, Koch T, Spieth PM, Uhlig C, Yaqub J, Bastin B, Geib J, Schaefer MS, Weiss M, Treschan TA, Reske AW, Simon P, Brodhun A, Ferner M, Hartmann E, Laufenberg-Feldmann R, Strys L, de Robertis E, Perilli V, Proietti R, Amantea B, Caroleo S, Tropea F, Bacuzzi A, Vanoni M, Cinnella G, Caggianelli G, D'Antini D, La Bella D, Mollica G, Cortegiani A, Giarratano A, Montalto F, Raineri SM, Barberis B, Celentano C, Grio M, Spagnolo L, Gratarola A, Molin A, Pellerano G, Pezzato S, Rusca R, Della Rocca G, Bos LDJ, Brunelli A, Marti A, Cegarra V, Merten A, Moral MV, Parera A, Unzueta MC, Sabaté S, Sierra P, Mayoral JF, Prieto M, Gil MG, Marín CM, Mills GH, Bodger P, Vidal Melo MF, Sulemanji D, Sprung J]] , High versus low positive end-expiratory pressure during general anaesthesia for open abdominal surgery (PROVHILO trial): a multicentre randomised controlled trial. LANCET 2014;384 (9942):495-503 [PubMed]
All Publications
Research programmes

Organprotection

In the range of cardioprotection we established different experimental models to investigate cardioprotective effects of anesthetics. 1) an in vivo model to study Sevoflurane-induced cardioprotection in hyperglycaemic rats, 2)  an in vitro model to determine the influence of different substrate conditions and preconditioning protocols on morphine-induced cardioprotection in the isolated rat heart and 3) Isolation of mitochondria from rat heart to identify the role of mitochondrial function in cardioprotection. Additionally, we evaluated the role of cardiac hexokinase in cell death. We examined how morphine affects cardiac hexokinase, and its relation to anesthesia, preconditioning, and genetic manipulation. Furthermore in collaboration with Rotterdam (EG Mik) we determined for the first time ever liver mitochondrial oxygen tension in vivo. Clinically, we studied how insulin therapy affects the neurohumoral stress response in cardiac surgery patients. With regard to anesthetics and intracellular signal transduction we were able to show that local anesthetics (LA) inhibit the activation of human NMDA receptors in a concentration dependent manner, independent of the charge of the LA and by an intracellular mechanism most likely mediated via inhibition of proteinkinase C (PKC). This effect may contribute to reduced hyperalgesia and opiate tolerance observed after systemic administration of LA.
Theme: Immunity & Infection

Faculty
Prof. dr. M.W. Hollmann MD PhD
Dr. J. Frässdorf MD PhD
Dr. N.C. Hauck-Weber PhD
Prof. dr. S.G. de Hert MD
Dr. J. Horn MD PhD
Prof. dr. R.A. van Hulst MD PhD (Royal Netherlands Navy)
Prof. N.P. Juffermans MD PhD
Dr. P.B. Lirk MSc
Prof. dr. B. Preckel MA MD PhD
Prof. W.S. Schlack MD PhD
Prof. M.J. Schultz MD PhD
Dr. M.F. Stevens MD PhD
Dr. D.P. Veelo MD PhD
Dr. J.H. Vranken
Dr. H.C.H. Wartenberg
Dr. J.T. Wegener MD PhD
Dr. ir. C.J. Zuurbier PhD
Dr. J.K. Zuur MD PhD

Postdocs
Dr. H.J. Avis MD
Dr. M.M. Berger (University of Heidelberg)
Dr. L.D.J. Bos
Dr. J. Hermanides MD PhD
Dr. J. Kessler MD (University of Heidelberg)
Dr. A.P.J Vlaar MD PhD

PhD Students
Drs. J. Bosboom
Drs. E.M.E. Bos MD
Drs. D. Brevoord MD
Drs. C.W. Doornebal (NKI)
Drs. G.J. Glas
M. van Haperen
Dr. H. Hermanns MD
L. Hol
Drs. W. ten Hoope
Drs. A.H. Hulst MD
S.W. de Jonge
D.J.B. Kleinveld
Drs. L. Koers
T.H. Mungroop MD MSc
S.G.L.H. Nijbroek
Dr. S. Picardi MD
Drs. L.M. Posthuma MD
Drs. J.L. van Schuppen
A. Smorenberg
L.E. Terwindt
Drs. L. Uthman
M. Wijnberge
T.T. Wingelaar MD (Royal Netherlands Navy)
Y. Xiao PhD
M.L. van Zuylen
G. Balestra MD
Drs. F.M. de Beer
Dr. B.L.P. Bloemers
M. Boshuizen MSc (Doctor)
Drs. J.S. Breel (Diak Utrecht)
Drs. J. Faker MSc
Drs. M.B.A. Harmon MD
Drs. H.J.F. Helmerhorst MD (Leiden University Medical Center)
Drs. L. Hemmik MD
Drs. M.E. van Hezel MSc (Doctor)
Drs. S.M. van der Hoeven
Drs. J. Juschten
Drs. B.J. Ruijter (Technical University Twente)
Drs. L.R.A. Schouten
F.D. Simonis MD
F. van Someren Gréve MD
Drs. J. Sommers MD
Drs. M.F.M. van Stijn BSc MD MSc
Drs. C.J. Wensing (Diak Utrecht)
Drs. F. Wille MD
Drs. X. Zuidema (Diak Utrecht)

Others
J.F. Buchner-Doeven
A. Van der Graaf
J. Hoeksma
R.P. Kerindongo
Drs. M. Kronkel
A. Tuip-de Boer
Y. Wang MD

Dr. J. Horn MD PhD (Neurology on the Intensive Care Unit)

Prof. M.J. Schultz MD PhD (Prevention and treatment of organ failure in intensive care medicine)

Dr. A.P.J Vlaar MD PhD (Transfusion related morbidity and mortality)

Prof. N.P. Juffermans MD PhD (Pathogenesis and treatment of organ failure in the critically ill)

Dr. ir. C.J. Zuurbier PhD (Cell survival mechanisms in acute and chronic cardiovascular pathology)

Other research related activities
  • Membership of advisory board / Consultant, Eurocept
  • Contribution to guidelines and protocols, Perioperative Pain therapy
  • Contribution to training of professionals, Schering Plough BV
  • Membership of editorial board / Editorship, ANESTHESIA AND ANALGESIA
  • Membership of editorial board / Editorship, NEDERLANDS TIJDSCHRIFT VOOR ANESTHESIOLOGIE
  • Speakers bureau / Lectures, Abbott BV
  • Speakers bureau / Lectures, Pfizer BV
  • Speakers bureau / Lectures, Schering Plough BV
  • Speakers bureau / Lectures, BBraun
  • Membership of medical or scientific committee, NvA, Research Committee
  • Membership of medical or scientific committee, German Society of Anesthesia & Intensive Care (DGAI), Research Committee
  • Membership of medical or scientific committee, European Society of Anaesthesology (ESA), Subcommittee on Regioanl Anaesthesia & Local Anaesthesia
  • Contribution to guidelines and protocols, Ultrasound guided Regional Anaesthesia
  • Membership of medical or scientific committee, Expert Group on Ultrasound in Regional Anaesthesia, Expert Panel (ZUERS)
  • Membership of advisory board / Consultant, ECHO
Current research funding
  • B. Braun Melsungen AG
  • Edwards Lifesciences
  • Medtronic Europe Sarl
  • Ministerie van Defensie (Kerkrade)
  • Orion Clinical Services Limited
  • Stichting Global Ichthyosis Syndrome Network
  • ZonMw