- Chang Jung-Chin, Go Simei, de Waart Dirk R., Munoz-Garrido Patricia, Beuers Ulrich, Paulusma Coen C., Oude Elferink Ronald Soluble Adenylyl Cyclase Regulates Bile Salt-Induced Apoptosis in Human Cholangiocytes Hepatology (Baltimore, Md.) 2016;64 (2):522-534 [PubMed]
- Kremer Andreas E., Bolier Ruth, Dixon Peter H., Geenes Victoria, Chambers Jenny, Tolenaars Dagmar, Ris-Stalpers Carrie, Kaess Bernhard M., Rust Christian, van der Post Joris A., Williamson Catherine, Beuers Ulrich, Oude Elferink Ronald P. J. Autotaxin activity has a high accuracy to diagnose intrahepatic cholestasis of pregnancy Journal of hepatology 2015;62 (4):897-904 [PubMed]
- Kunne Cindy, Acco Alexandra, Hohenester Simon, Duijst Suzanne, de Waart Dirk R., Zamanbin Alaleh, Oude Elferink Ronald P. J. Defective bile salt biosynthesis and hydroxylation in mice with reduced cytochrome P450 activity Hepatology (Baltimore, Md.) 2013;57 (4):1509-1517 [PubMed]
- Groen Annemiek, Romero Marta Rodriguez, Kunne Cindy, Hoosdally Sarah J., Dixon Peter H., Wooding Carol, Williamson Catherine, Seppen Jurgen, van den Oever Karin, Mok Kam S., Paulusma Coen C., Linton Kenneth J., Oude Elferink Ronald P. J. Complementary functions of the flippase ATP8B1 and the floppase ABCB4 in maintaining canalicular membrane integrity Gastroenterology 2011;141 (5):1927-37.e1-4 [PubMed]
- Kremer Andreas E., Martens Job J. W. W., Kulik Wim, Ruëff Franziska, Kuiper Edith M. M., van Buuren Henk R., van Erpecum Karel J., Kondrackiene Jurate, Prieto Jesus, Rust Christian, Geenes Victoria L., Williamson Catherine, Moolenaar Wouter H., Beuers Ulrich, Elferink Ronald P. J. Oude Lysophosphatidic Acid Is a Potential Mediator of Cholestatic Pruritus Gastroenterology 2010;139 (3):1008-U399 [PubMed]
'74 - '81 Study Biochemistry at the University of Amsterdam (graduation cum laude)
'81 - '85 PhD-student at the Dept. of Biochemistry University of Amsterdam (PhD. thesis: "Biosynthesis, transport and processing of lysosomal α-glucosidase" (promotor: prof. J.M. Tager)
'85 - '86 post-doc at the Biotechnological Center, University of Amsterdam (project: In vitro immunisation of B-cells for production of monoclonal antibodies)
'86 - '99 staff member (biochemist) of the Dept. of Gastrointestinal and Liver Diseases, Academic Medical Center, Amsterdam
'99 - '00 Associate professor and head of the Laboratory for Experimental Hepatology, Academic Medical Center, Amsterdam
2000-present Professor of Experimental Hepatology, Academic Medical Center Amsterdam
2002-present Head of the Tytgat Institute for Liver and Intestinal Research.
Prof. R.P.J. Oude Elferink PhD (Hepatic metabolism and transport processes)
1. Cholestatic pruritus. Patients with various forms of cholestasis often suffer from chronic itch (pruritus). This can be a very heavy burden to the patient. The mechanism of cholestatic itch is unknown. Bile salts and endogenous opioids have been implicated but we have found no evidence to support this contetnion. We have set up assays to analyze the effect of cholestatic factors on neuronal signalling. Currently, we are analyzing the factors that we have identified and we are trying to elucidate the mechanism by which they cause itch. This will provide means to implement existing drugs or develop new drugs against this agonizing problem (collaboration with prof. Ulrich Beuers).
2. The (patho)physiological function of soluble adenylyl cyclase (sAC). Soluble adenyl cyclase is an enzyme that synthesizes the potent signaling molecule cyclic AMP. sAC is the evolutionary oldest adenylyl cyclase but it has hardly been studied so far. We are investigating its role in regulation of apoptosis, the Warburg effect (in relation to cancer and immune cell metabolism), and barrier function of epithelial cells.It appears that sAC has a regulatory role in various important cell-autonomous functions
2. Hepatic transport functions. Many aspects of bile formation have been elucidated in the past decades. However, several aspects of normal bile formation and especially of biliary dyfunction and cholestasis are not well understood. We are improving animal models for cholestatic diseases; not only with respect to defects in transport function but also with respect to the role of bile salt signalling and the role of biliary bicarbonate secretion. In addition, we are trying to unravel the factors that contribute to the development of autoimmunitiy against bile duct epithelial cells as occurs in primary biliary cirrhosis (collaboration with prof. Ulrich Beuers)
This research group participates in the Amsterdam UMC Research Institutes: Amsterdam Gastroenterology & Metabolism as well as Cancer Center Amsterdam
R. Hoekstra PhD
- AMC (Vrijgesteld)
- KWF Kankerbestrijding
- Robarts Clinical Trials Inc.
- Stichting Dioraphte