Dr. J.H. Akkerdaas publications


Publications Dr. J.H. Akkerdaas

Main activities
Allergy research
Focus of research
  • Protein-chemistry and molecular biology of respiratory and food allergens: what makes an allergen an allergen?
  • Innovative approaches for in vitro and in vivo diagnosis of IgE-mediated allergy: from allergen extracts to molecular diagnostics.
  • Innovative biopharmaceutical approaches for allergen-specific immunotherapy: recombinant technology, novel adjuvants and administration routes.
  • Immuno-epidemiology of respiratory and food allergies: the role of allergen exposure, environment, diet, infections and lifestyle in the development of allergy.
  • Mouse models of allergy and asthma: how are sensitization and disease expression regulated?



  • Akkerdaas J, Totis M, Barnett B, Bell E, Davis T, Edrington T, Glenn K, Graser G, Herman R, Knulst A, Ladics G, McClain S, Poulsen LK, Ranjan R, Rascle JB, Serrano H, Speijer D, Wang R, Pereira Mouriès L, Capt A, van Ree R, Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure. CLIN TRANSL ALLERGY 2018;8:30 [PubMed]
  • Fu W, Gao Z, Gao L, Jin J, Liu M, Sun Y, Wu S, Wu L, Ma H, Dong Y, Wang X, Gao B, Wang H, Akkerdaas JH, Versteeg SA, van Ree R, Identification of a 62-kDa major allergen from Artemisia pollen as a putative galactose oxidase. ALLERGY 2018;73 (5):1041-1052 [PubMed]
  • Warners MJ, Terreehorst I, van den Wijngaard RM, Akkerdaas J, van Esch BCAM, van Ree R, Versteeg SA, Smout AJPM, Bredenoord AJ, Abnormal Responses to Local Esophageal Food Allergen Injections in Adult Patients With Eosinophilic Esophagitis. GASTROENTEROLOGY 2018;154 (1):57-60.e2 [PubMed]


  • Buhler S, Akkerdaas JH, A Pertinhez T, van Ree R, Dossena A, Sforza S, Tedeschi T, Development of a strategy for the total chemical synthesis of an allergenic protein: the peach LTP Pru p 3. J PEPT SCI 2017;23 (4):282-293 [PubMed]


  • Gao ZS, Ma YT, Zhou X, Yang ZW, Jia HJ, Gao L, Wu SD, Han LY, Yi XY, Wang HY, Akkerdaas JH, van Ree R, Quantification of Peach Fruit Allergen Lipid Transfer Protein by a Double Monoclonal Antibody-based Sandwich ELISA. FOOD ANAL METHOD 2016;9 (4):823-830
  • Gao ZS, Zhou X, Yang ZW, Versteeg SA, Gao L, Fu WY, Wang HY, Zhou JY, Akkerdaas JH, van Ree R, IgE-binding potencies of three peach Pru p 1 isoforms. MOL NUTR FOOD RES 2016;60 (11):2457-2466 [PubMed]


  • Utsch L, Folisi C, Akkerdaas JH, Logiantara A, van de Pol MA, van der Zee JS, Krop EJM, Lutter R, van Ree R, van Rijt LS, Allergic sensitization is associated with inadequate antioxidant responses in mice and men. ALLERGY 2015;70 (10):1246-1258 [PubMed]
  • van Rhijn BD, Vlieg-Boerstra BJ, Versteeg SA, Akkerdaas JH, van Ree R, Terreehorst I, Sprikkelman AB, Verheij J, Smout AJPM, Bredenoord AJ, Evaluation of allergen-microarray-guided dietary intervention as treatment of eosinophilic esophagitis. J ALLERGY CLIN IMMUN 2015;136 (4):1095-7.e3 [PubMed]
  • Zuidmeer-Jongejan L, Huber H, Swoboda I, Rigby N, Versteeg SA, Jensen BM, Quaak S, Akkerdaas JH, Blom L, Asturias J, Bindslev-Jensen C, Bernardi ML, Clausen M, Ferrara R, Hauer M, Heyse J, Kopp S, Kowalski ML, Lewandowska-Polak A, Linhart B, Maderegger B, Maillere B, Mari A, Martinez A, Mills ENC, Neubauer A, Nicoletti C, Papadopoulos NG, Portoles A, Ranta-Panula V, Santos-Magadan S, Schnoor HJ, Sigurdardottir ST, Stahl-Skov P, Stavroulakis G, Stegfellner G, Vázquez-Cortés S, Witten M, Stolz F, Poulsen LK, Fernandez-Rivas M, Valenta R, van Ree R, Development of a hypoallergenic recombinant parvalbumin for first-in-man subcutaneous immunotherapy of fish allergy. INT ARCH ALLERGY IMM 2015;166 (1):41-51 [PubMed]


  • Burney PGJ, Potts J, Kummeling I, Mills ENC, Clausen M, Dubakiene R, Barreales L, Fernandez-Perez C, Fernandez-Rivas M, Le TM, Knulst AC, Kowalski ML, Lidholm J, Ballmer-Weber BK, Braun-Fahlander C, Mustakov T, Kralimarkova T, Popov T, Sakellariou A, Papadopoulos NG, Versteeg SA, Zuidmeer L, Akkerdaas JH, Hoffmann-Sommergruber K, van Ree R, The prevalence and distribution of food sensitization in European adults. ALLERGY 2014;69 (3):365-371 [PubMed]
  • Zuidmeer-Jongejan L, Fernández-Rivas M, Winter MGT, Akkerdaas JH, Summers C, Lebens A, Knulst AC, Schilte P, Briza P, Gadermaier G, van Ree R, Oil body-associated hazelnut allergens including oleosins are underrepresented in diagnostic extracts but associated with severe symptoms. CLIN TRANSL ALLERGY 2014;4 (1):4 [PubMed]


  • Amoah AS, Obeng BB, Larbi IA, Versteeg SA, Aryeetey Y, Akkerdaas JH, Zuidmeer L, Lidholm J, Fernández-Rivas M, Hartgers FC, Boakye DA, van Ree R, Yazdanbakhsh M, Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity. J ALLERGY CLIN IMMUN 2013;132 (3):639-647 [PubMed]
  • Masthoff LJN, Mattsson L, Zuidmeer-Jongejan L, Lidholm J, Andersson K, Akkerdaas JH, Versteeg SA, Garino C, Meijer Y, Kentie P, Versluis A, den Hartog Jager CF, Bruijnzeel-Koomen CAFM, Knulst AC, van Ree R, van Hoffen E, Pasmans SGMA, Sensitization to Cor a 9 and Cor a 14 is highly specific for a hazelnut allergy with objective symptoms in Dutch children and adults. J ALLERGY CLIN IMMUN 2013;132 (2):393-399 [PubMed]


  • Akkerdaas J, Finkina EI, Balandin SV, Santos Magadán S, Knulst A, Fernandez-Rivas M, Asero R, van Ree R, Ovchinnikova TV, Lentil (Lens culinaris) Lipid Transfer Protein Len c 3: A Novel Legume Allergen. INT ARCH ALLERGY IMM 2012;157 (1):51-57 [PubMed]


  • van Zuuren EJ, Terreehorst I, Tupker RA, Hiemstra PS, Akkerdaas JH, Anaphylaxis after consuming soy products in patients with birch pollinosis. ALLERGY 2010;65 (10):1348-1349 [PubMed]


  • Flinterman AE, Akkerdaas JH, den Hartog Jager CF, Rigby NM, Fernandez-Rivas M, Hoekstra MO, Bruijnzeel-Koomen CA, Knulst AC, van Ree R, Pasmans SG, Lipid transfer protein-linked hazelnut allergy in children from a non-Mediterranean birch-endemic area. J ALLERGY CLIN IMMUN 2008;121 (2):423-428.e2 [PubMed]
  • Flinterman AE, Akkerdaas JH, Knulst AC, van Ree R, Pasmans SG, Hazelnut allergy: from pollen-associated mild allergy to severe anaphylactic reactions. CURR OPIN ALLERGY CL 2008;8 (3):261-265 [PubMed]
  • Marsh J, Rigby N, Wellner K, Reese G, Knulst A, Akkerdaas J, van Ree R, Radauer C, Lovegrove A, Sancho A, Mills C, Vieths S, Hoffmann-Sommergruber K, Shewry PR, Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis. MOL NUTR FOOD RES 2008;52 (Suppl. 2):S272-S285 [PubMed]
  • Rigby NM, Marsh J, Sancho AI, Wellner K, Akkerdaas J, van Ree R, Knulst A, Fernández-Rivas M, Brettlova V, Schilte PP, Summer C, Pumphrey R, Shewry PR, Mills ENC, The purification and characterisation of allergenic hazelnut seed proteins. MOL NUTR FOOD RES 2008;52 (Suppl. 2):S251-S261 [PubMed]


  • Vassilopoulou EV, Zuidmeer L, Akkerdaas J, Rigby N, Moren FJ, Papadopoulos NG, Saxoni-Papageorgiou P, Milis C, van Ree R, Optimized techniques for the extraction of grape allergens appropriate for in vivo and in vitro testing and diagnosis. MOL NUTR FOOD RES 2007;51 (3):360-366 [PubMed]
  • Vassilopoulou E, Zuidmeer L, Akkerdaas J, Tassios I, Rigby NR, Mills ENC, van Ree R, Saxoni-Papageorgiou P, Papadopoulos NG, Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome. INT ARCH ALLERGY IMM 2007;143 (2):92-102 [PubMed]


  • Akkerdaas JH, Schocker F, Vieths S, Versteeg S, Zuidmeer L, Hefle SL, Aalberse RC, Richter K, Ferreira F, van Ree R, Cloning of oleosin, a putative new hazelnut allergen, using a hazelnut cDNA library. MOL NUTR FOOD RES 2006;50 (1):18-23 [PubMed]
  • Flinterman AE, Hoekstra MO, Meijer Y, van Ree R, Akkerdaas JH, Bruijnzeel-Koomen CA, Knulst AC, Pasmans SG, Clinical reactivity to hazelnut in children: association with sensitization to birch pollen or nuts?. J ALLERGY CLIN IMMUN 2006;118 (5):1186-1189 [PubMed]
  • Vassilopoulou E, Rigby N, Moreno FJ, Zuidmeer L, Akkerdaas J, Tassios I, Papadopoulos NG, Saxoni-Papageorgiou P, van Ree R, Mills C, Effect of in vitro gastric and duodenal digestion on the allergenicity of grape lipid transfer protein. J ALLERGY CLIN IMMUN 2006;118 (2):473-480 [PubMed]


  • Akkerdaas JH. Identification and quantification of hazelnut allergens. s.l.: s.n.; 2005. 176p. ISBN 90-6464-243-5 [UvA Dissertations Online] Proefschrift Universiteit van Amsterdam. (Promotor(s): Aalberse RC; copromotor(s): van Ree R)
  • Akkerdaas JH, Wensing M, Asero R, Fernandez Rivas M, Rivas MF, Knulst AC, Bolhaar S, Hefle SL, Aalberse RC, van Ree R, IgE binding to pepsin-digested food extracts. INT ARCH ALLERGY IMM 2005;138 (3):203-208 [PubMed]
  • Gilissen LJWJ, Bolhaar STHP, Matos CI, Rouwendal GJA, Boone MJ, Krens FA, Zuidmeer L, van Leeuwen A, Akkerdaas J, Hoffmann-Sommergruber K, Knulst AC, Bosch D, van de Weg WE, van Ree R, Silencing the major apple allergen Mal d 1 by using the RNA interference approach. J ALLERGY CLIN IMMUN 2005;115 (2):364-369 [PubMed]
  • Zuidmeer L, van Leeuwen WA, Budde IK, Cornelissen J, Bulder I, Rafalska I, Besolí NT, Akkerdaas JH, Asero R, Fernandez Rivas M, Rivas MF, Gonzalez Mancebo E, Mancebo EG, van Ree R, Lipid transfer proteins from fruit: cloning, expression and quantification. INT ARCH ALLERGY IMM 2005;137 (4):273-281 [PubMed]


  • Akkerdaas JH, Wensing M, Knulst AC, Stephan O, Hefle SL, Aalberse RC, van Ree R, A novel approach for the detection of potentially hazardous pepsin stable hazelnut proteins as contaminants in chocolate-based food. J AGR FOOD CHEM 2004;52 (25):7726-7731 [PubMed]
  • Mittag D, Akkerdaas J, Ballmer-Weber BK, Vogel L, Wensing M, Becker WM, Koppelman SJ, Knulst AC, Helbling A, Hefle SL, van Ree R, Vieths S, Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J ALLERGY CLIN IMMUN 2004;114 (6):1410-1417 [PubMed]
  • Schocker F, Lüttkopf D, Scheurer S, Petersen A, Cisteró-Bahima A, Enrique E, San Miguel-Moncín M, Akkerdaas J, van Ree R, Vieths S, Becker WM, Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy. J ALLERGY CLIN IMMUN 2004;113 (1):141-147 [PubMed]


  • Akkerdaas JH, Wensing M, Knulst AC, Aalberse RC, Hefle SL, van Ree R, In vitro and in vivo characterization of hazelnut skin prick test extracts. ARB PAUL EHRLICH INST BUNDESAMT SERA IMPFSTOFFE FRANKF A M 2003;ahead of print [PubMed]
  • Akkerdaas JH, Wensing M, Knulst AC, Krebitz M, Breiteneder H, de Vries S, Penninks AH, Aalberse RC, Hefle SL, van Ree R, How accurate and safe is the diagnosis of hazelnut allergy by means of commercial skin prick test reagents?. INT ARCH ALLERGY IMM 2003;132 (2):132-140 [PubMed]


  • Wensing M, Akkerdaas JH, van Leeuwen WA, Stapel SO, Bruijnzeel-Koomen CAFM, Aalberse RC, Bast BJEG, Knulst AC, van Ree R, IgE to Bet v 1 and profilin: cross-reactivity patterns and clinical relevance. J ALLERGY CLIN IMMUN 2002;110 (3):435-442 [PubMed]
  • Wensing M, Penninks AH, Hefle SL, Akkerdaas JH, van Ree R, Koppelman SJ, Bruijnzeel-Koomen CAFM, Knulst AC, The range of minimum provoking doses in hazelnut-allergic patients as determined by double-blind, placebo-controlled food challenges. CLIN EXP ALLERGY 2002;32 (12):1757-1762 [PubMed]


  • Aalberse RC, Akkerdaas J, van Ree R, Cross-reactivity of IgE antibodies to allergens. ALLERGY 2001;56 (6):478-490 [PubMed]
  • Asero R, Mistrello G, Roncarolo D, de Vries SC, Gautier MF, Ciurana CL, Verbeek E, Mohammadi T, Knul-Brettlova V, Akkerdaas JH, Bulder I, Aalberse RC, van Ree R, Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. INT ARCH ALLERGY IMM 2001;124 (1-3):67-69 [PubMed]


  • Asero R, Mistrello G, Roncarolo D, de Vries SC, Gautier MF, Ciurana CL, Verbeek E, Mohammadi T, Knul-Brettlova V, Akkerdaas JH, Bulder I, Aalberse RC, van Ree R, Lipid transfer protein: a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion. INT ARCH ALLERGY IMM 2000;122 (1):20-32 [PubMed]
  • van Ree R, Cabanes-Macheteau M, Akkerdaas J, Milazzo JP, Loutelier-Bourhis C, Rayon C, Villalba M, Koppelman S, Aalberse R, Rodriguez R, Faye L, Lerouge P, Beta(1,2)-xylose and alpha(1,3)-fucose residues have a strong contribution in IgE binding to plant glycoallergens. J BIOL CHEM 2000;275 (15):11451-11458 [PubMed]


  • van Ree R, van Leeuwen WA, Akkerdaas JH, Aalberse RC, How far can we simplify in vitro diagnostics for Fagales tree pollen allergy? A study with three whole pollen extracts and purified natural and recombinant allergens. CLIN EXP ALLERGY 1999;29 (6):848-855 [PubMed]


  • van der Veen MJ, van Ree R, Aalberse RC, Akkerdaas J, Koppelman SJ, Jansen HM, van der Zee JS, Poor biologic activity of cross-reactive IgE directed to carbohydrate determinants of glycoproteins. J ALLERGY CLIN IMMUN 1997;100 (3):327-334 [PubMed]


  • van Ree R, Antonicelli L, Akkerdaas JH, Garritani MS, Aalberse RC, Bonifazi F, Possible induction of food allergy during mite immunotherapy. ALLERGY 1996;51 (2):108-113 [PubMed]
  • van Ree R, Antonicelli L, Akkerdaas JH, Pajno GB, Barberio G, Corbetta L, Ferro G, Zambito M, Garritani MS, Aalberse RC, Bonifazi F, Asthma after consumption of snails in house-dust-mite-allergic patients: a case of IgE cross-reactivity. ALLERGY 1996;51 (6):387-393 [PubMed]
  • Witteman AM, Voorneman R, van den Oudenrijn S, van Leeuwen J, Akkerdaas J, van der Zee JS, Aalberse RC, Silverfish protein in house dust in relation to mite and total arthropod level. CLIN EXP ALLERGY 1996;26 (10):1171-1176 [PubMed]


  • Akkerdaas JH, van Ree R, Aalbers M, Stapel SO, Aalberse RC, Multiplicity of cross-reactive epitopes on Bet v I as detected with monoclonal antibodies and human IgE. ALLERGY 1995;50 (3):215-220 [PubMed]
  • de Maat-Bleeker F, Akkerdaas JH, van Ree R, Aalberse RC, Vineyard snail allergy possibly induced by sensitization to house-dust mite (Dermatophagoides pteronyssinus). ALLERGY 1995;50 (5):438-440 [PubMed]
  • Witteman AM, van den Oudenrijn S, van Leeuwen J, Akkerdaas J, van der Zee JS, Aalberse RC, IgE antibodies reactive with silverfish, cockroach and chironomid are frequently found in mite-positive allergic patients. INT ARCH ALLERGY IMM 1995;108 (2):165-169 [PubMed]


  • Witteman AM, Akkerdaas JH, van Leeuwen J, van der Zee JS, Aalberse RC, Identification of a cross-reactive allergen (presumably tropomyosin) in shrimp, mite and insects. INT ARCH ALLERGY IMM 1994;105 (1):56-61 [PubMed]