Dr. N.N. van der Wel publications


Publications Dr. N.N. van der Wel

Scientific staff member (UD)
Main activities
Research, Teaching
Head Electron Microscopy Center Amsterdam
Focus of research

Focus of research
The research questions in the lab focuses on the understanding of Mycobacterium tuberculosis infections. This bacterium is responsible for one of the biggest killers causing the deadly disease tuberculosis. The vaccine currently used to prevent M.tuberculosis infections is M. bovis BCG. This live vaccine was developed almost 1 century ago and has not been improved ever since. We collaborate with several groups worldwide (Germany, France, Spain, Swiss, USA, South Africa) to improve this vaccine and understand the biology of tuberculosis infection. The subcellular localization of the pathogenic bacteria within cultured cells and in vivo is an important aspect (van der Wel et al., Cell 2007; Abdallah et al JI 2008; Abdallah et al., JI, 2011; Houben et al., Cell Microbiol. 2012; Weerdenburg et al., Cell Microbiol. 2012; Stoop et al; Cell Microbiol. 2013) and subject of our ongoing research. In addition, we discovered that the outermost layer of mycobacteria consists of a labile structure that contains several secreted products, involved in the immune responses of pathogenic bacteria (Sani et al 2010, Ates 2016). Currently we are investigating the mechanism of capsular stability and generation, as this is the first encounter of the vaccine BCG with the host. The projects serves both fundamental innovative research and has a clear application, in the vaccine manufacturing.

Electron Microscopy

At the Core facility Cellular Imaging, several different microscopy techniques are made available for the AMC-staff, and affiliated researchers (http://www.cellularimaging.nl). Since March 2014 Dr. Nicole van der Wel was appointed head Electron Microscopy. With a group of 4 technicians (Daisy Picavet, Henk van der Veen, Anita Grootemaat and Edwin Scholl) we assist researchers from the AMC and partners with performing Electron Microscopy. We give advice on the techniques needed, support performing and interpretation of the experiments.

November 2015 we opened the Electron Microscopy Centre Amsterdam (EMCA http://www.cellularimaging.nl/electron-microscopy/). The EMCA is a collaboration between several Amsterdam life science research institutes, including the Vumc, AMC, NKI, ACTA and NIN, and is housed at Core facility Cellular Imaging within the department of Cell Biology&Histology at the AMC. At the EMCA the operators and researchers from the different universities and institutes work on the various TEM and SEM microscopes and have shared work-discussions. The shared equipment is paid for by the involved partners as well as by various other academic and industrial users. As such we collaborate with most Amsterdam and various (inter)national research groups and have created an EM knowledge centre in Amsterdam. We have long standing expertise in transmission electron microscopy (TEM), scanning electron microscopy (SEM), but also apply new developments such as combined light electron microscopy (CLEM), tomography, immunoEM, and Cryo-EM.


  • Claushuis TAM, de Stoppelaar SF, de Vos AF, Grootemaat AE, van der Wel NN, Roelofs J, Ware J, van't Veer C, van der Poll T, Nbeal2 Deficiency Increases Organ Damage but Does Not Affect Host Defense During Gram-Negative Pneumonia-Derived Sepsis. ARTERIOSCL THROM VAS 2018;38 (8):1772-1784 [PubMed]
  • Claushuis TAM, van der Donk LEH, Luitse AL, van Veen HA, van der Wel NN, van Vught LA, Roelofs JJTH, de Boer OJ, Lankelma JM, Boon L, de Vos AF, van 't Veer C, van der Poll T, Role of Peptidylarginine Deiminase 4 in Neutrophil Extracellular Trap Formation and Host Defense during Klebsiella pneumoniae-Induced Pneumonia-Derived Sepsis. J IMMUNOL 2018;201 (4):1241-1252 [PubMed]
  • Michailidou I , Jongejan A, Vreijling JP, Georgakopoulou T, de Wissel MB, Wolterman RA, Ruizendaal P, Klar-Mohamad N, Grootemaat AE, Picavet DI, Kumar V, van Kooten C, Woodruff TM, Morgan BP, van der Wel NN, Ramaglia V, Fluiter K, Baas F, Systemic inhibition of the membrane attack complex impedes neuroinflammation in chronic relapsing experimental autoimmune encephalomyelitis. ACTA NEUROPATHOL COM 2018;6 (1):36 [PubMed]
  • Palao T, Medzikovic L, Rippe C, Wanga S, Al-Mardini C, van Weert A, de Vos J, van der Wel NN, van Veen HA, van Bavel ET, Swärd K, de Waard V, Bakker EN, Thrombospondin-4 mediates cardiovascular remodelling in angiotensin II-induced hypertension. CARDIOVASC PATHOL 2018;35:12-19 [PubMed]
  • Scutigliani EM, Scholl ER, Grootemaat AE, Khanal S, Kochan JA, Krawczyk PM, Reits EA, Garzan A, Ngo HX, Green KD, Garneau-Tsodikova S, Ruijter JM, van Veen HA, van der Wel NN, Interfering With DNA Decondensation as a Strategy Against Mycobacteria. FRONT MICROBIOL 2018;9:2034 [PubMed]
  • van Leeuwen LM, Boot M, Kuijl C, Picavet DI, van den Brink G, van der Pol SMA, de Vries HE, van der Wel NN, van der Kuip M, van Furth AM, van der Sar AM, Bitter W, Mycobacteria employ two different mechanisms to cross the blood-brain barrier. CELL MICROBIOL 2018;20 (9):e12858 [PubMed]
  • Yu Y, Gool E, Berckmans RJ, Coumans FAW, Barendrecht AD, Maas C, van der Wel NN, Altevogt P, Sturk A, Nieuwland R, Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets. J THROMB HAEMOST 2018;16 (6):1153-1163 [PubMed]


  • Bedussi B, van der Wel NN, de Vos J, van Veen H, Siebes M, VanBavel E, Bakker ENTP, Paravascular channels, cisterns, and the subarachnoid space in the rat brain: A single compartment with preferential pathways. J CEREBR BLOOD F MET 2017;37 (4):1374-1385 [PubMed]
  • Beldman TJ, Senders ML, Alaarg A, Pérez-Medina C, Tang J, Zhao Y, Fay F, Deichmöller J, Born B, Desclos E, van der Wel NN, Hoebe RA, Kohen F, Kartvelishvily E, Neeman M, Reiner T, Calcagno C, Fayad ZA, de Winther MPJ, Lutgens E, Mulder WJM, Kluza E, Hyaluronan Nanoparticles Selectively Target Plaque-Associated Macrophages and Improve Plaque Stability in Atherosclerosis. ACS NANO 2017;11 (6):5785-5799 [PubMed]
  • Gao Y, Vidal-Itriago A, Kalsbeek MJ, Layritz C, García-Cáceres C, Tom RZ, Eichmann TO, Vaz FM, Houtkooper RH, van der Wel N, Verhoeven AJ, Yan J, Kalsbeek A, Eckel RH, Hofmann SM, Yi CX, Lipoprotein Lipase Maintains Microglial Innate Immunity in Obesity. CELL REP 2017;20 (13):3034-3042 [PubMed]
  • Maat DS, Biggs T, Evans C, van Bleijswijk JDL, van der Wel NN, Dutilh BE, Brussaard CPD, Characterization and Temperature Dependence of Arctic Micromonas polaris Viruses. VIRUSES-BASEL 2017;9 (6):134 [PubMed]
  • van Zon M, de Punder K, van der Sar A, Brosch R, Pando RH, Grootemaat A , Tigchelaar-Gutter W, van der Wel N, Subcellular localization of M. tuberculosis in vivo and effect of the adaptive immunity. ULTRASTRUCT PATHOL 2017;41 (1):133 [PubMed]


  • Abhyankar WR, Kamphorst K, Swarge BN, van Veen H, van der Wel NN, Brul S, de Koster CG, de Koning LJ, The Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores. FRONT MICROBIOL 2016;7:1636 [PubMed]
  • Ates LS, van der Woude AD, Bestebroer J, van Stempvoort G, Musters RJP, Garcia-Vallejo JJ, Picavet DI, Weerd Rvd, Maletta M, Kuijl CP, van der Wel NN, Bitter W, The ESX-5 System of Pathogenic Mycobacteria Is Involved In Capsule Integrity and Virulence through Its Substrate PPE10. PLOS PATHOG 2016;12 (6):e1005696 [PubMed]
  • Potze L, Di Franco S, Grandela C, Pras-Raves ML, Picavet DI, van Veen HA, van Lenthe H, Mullauer FB, van der Wel NN, Luyf A, van Kampen AHC, Kemp S, Everts V, Kessler JH, Vaz FM, Medema JP, Betulinic acid induces a novel cell death pathway that depends on cardiolipin modification. ONCOGENE 2016;35 (4):427-437 [PubMed]
  • Ribeiro CMS, Sarrami-Forooshani R, Setiawan LC, Zijlstra-Willems EM, van Hamme JL, Tigchelaar W, van der Wel NN, Kootstra NA, Gringhuis SI, Geijtenbeek TBH, Receptor usage dictates HIV-1 restriction by human TRIM5α in dendritic cell subsets. NATURE 2016;540 (7633):448-452 [PubMed]
  • van der Spek AH, Bloise FF, Tigchelaar W, Dentice M, Salvatore D, van der Wel NN, Fliers E, Boelen A, The Thyroid Hormone Inactivating Enzyme Type 3 Deiodinase is Present in Bactericidal Granules and the Cytoplasm of Human Neutrophils. ENDOCRINOLOGY 2016;157 (8):3293-3305 [PubMed]
  • Zheng L, Abhyankar W, Ouwerling N, Dekker HL, van Veen H, van der Wel NN, Roseboom W, de Koning LJ, Brul S, de Koster CG, Bacillus subtilis Spore Inner Membrane Proteome. J PROTEOME RES 2016;15 (2):585-594 [PubMed]


  • Daleke-Schermerhorn MH, Felix T, Soprova Z, ten Hagen-Jongman CM, Vikström D, Majlessi L, Beskers J, Follmann F, de Punder K, van der Wel NN, Baumgarten T, Pham TV, Piersma SR, Jiménez CR, van Ulsen P, de Gier JW, Leclerc C, Jong WSP, Luirink J, Decoration of outer membrane vesicles with multiple antigens by using an autotransporter approach. APPL ENVIRON MICROB 2014;80 (18):5854-5865 [PubMed]
  • Jong WSP, Daleke-Schermerhorn MH, Vikström D, ten Hagen-Jongman CM, de Punder K, van der Wel NN, van de Sandt CE, Rimmelzwaan GF, Follmann F, Agger EM, Andersen P, de Gier JW, Luirink J, An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines. MICROB CELL FACT 2014;13:162 [PubMed]


  • Stoop EJM, Mishra AK, Driessen NN, van Stempvoort G, Bouchier P, Verboom T, van Leeuwen LM, Sparrius M, Raadsen SA, van Zon M, van der Wel NN, Besra GS, Geurtsen J, Bitter W, Appelmelk BJ, van der Sar AM, Mannan core branching of lipo(arabino)mannan is required for mycobacterial virulence in the context of innate immunity. CELL MICROBIOL 2013;15 (12):2093-2108 [PubMed]
  • van der Woude AD, Mahendran KR, Ummels R, Piersma SR, Pham TV, Jiménez CR, de Punder K, van der Wel NN, Winterhalter M, Luirink J, Bitter W, Houben ENG, Differential detergent extraction of mycobacterium marinum cell envelope proteins identifies an extensively modified threonine-rich outer membrane protein with channel activity. J BACTERIOL 2013;195 (9):2050-2059 [PubMed]


  • Houben D, Demangel C, van Ingen J, Perez J, Baldeón L, Abdallah AM, Caleechurn L, Bottai D, van Zon M, de Punder K, van der Laan T, Kant A, Bossers-de Vries R, Willemsen P, Bitter W, van Soolingen D, Brosch R, van der Wel N, Peters PJ, ESX-1-mediated translocation to the cytosol controls virulence of mycobacteria. CELL MICROBIOL 2012;14 (8):1287-1298 [PubMed]
  • Jong WSP, Soprova Z, de Punder K, ten Hagen-Jongman CM, Wagner S, Wickström D, de Gier JW, Andersen P, van der Wel NN, Luirink J, A structurally informed autotransporter platform for efficient heterologous protein secretion and display. MICROB CELL FACT 2012;11:85 [PubMed]
  • Weerdenburg EM, Abdallah AM, Mitra S, de Punder K, van der Wel NN, Bird S, Appelmelk BJ, Bitter W, van der Sar AM, ESX-5-deficient Mycobacterium marinum is hypervirulent in adult zebrafish. CELL MICROBIOL 2012;14 (5):728-739 [PubMed]


  • Abdallah AM, Bestebroer J, Savage NDL, de Punder K, van Zon M, Wilson L, Korbee CJ, van der Sar AM, Ottenhoff THM, van der Wel NN, Bitter W, Peters PJ, Mycobacterial Secretion Systems ESX-1 and ESX-5 Play Distinct Roles in Host Cell Death and Inflammasome Activation. J IMMUNOL 2011;187 (9):4744-4753 [PubMed]
  • Daleke MH, Cascioferro A, de Punder K, Ummels R, Abdallah AM, van der Wel N, Peters PJ, Luirink J, Manganelli R, Bitter W, Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) Protein Domains Target LipY Lipases of Pathogenic Mycobacteria to the Cell Surface via the ESX-5 Pathway. J BIOL CHEM 2011;286 (21):19024-19034 [PubMed]


  • Sani M, Houben ENG, Geurtsen J, Pierson J, de Punder K, van Zon M, Wever B, Piersma SR, Jiménez CR, Daffé M, Appelmelk BJ, Bitter W, van der Wel N, Peters PJ, Direct visualization by cryo-EM of the mycobacterial capsular layer: a labile structure containing ESX-1-secreted proteins. PLOS PATHOG 2010;6 (3):e1000794 [PubMed]
  • Weerdenburg EM, Peters PJ, van der Wel NN, How do mycobacteria activate CD8(+) T cells?. TRENDS MICROBIOL 2010;18 (1):1-10 [PubMed]


  • Abdallah AM, Savage NDL, van Zon M, Wilson L, Vandenbroucke-Grauls CMJE, van der Wel NN, Ottenhoff THM, Bitter W, The ESX-5 Secretion System of Mycobacterium marinum Modulates the Macrophage Response. J IMMUNOL 2008;181 (10):7166-7175 [PubMed]
  • Hava DL, van der Wel N, Cohen N, Dascher CC, Houben D, León L, Agarwal S, Sugita M, van Zon M, Kent SC, Shams H, Peters PJ, Brenner MB, Evasion of peptide, but not lipid antigen presentation, through pathogen-induced dendritic cell maturation. P NATL ACAD SCI USA 2008;105 (32):11281-11286 [PubMed]


  • van der Wel N, Hava D, Houben D, Fluitsma D, van Zon M, Pierson J, Brenner M, Peters PJ, M-tuberculosis and M-leprae translocate from the phagolysosome to the cytosol in myeloid cells. CELL 2007;129 (7):1287-1298 [PubMed]


  • Angénieux C, Fraisier V, Maître B, Racine V, van der Wel N, Fricker D, Proamer F, Sachse M, Cazenave JP, Peters P, Goud B, Hanau D, Sibarita JB, Salamero J, de la Salle H, The cellular pathway of CD1e in immature and maturing dendritic cells. TRAFFIC 2005;6 (4):286-302 [PubMed]
  • Boes M, van der Wel N, Peperzak V, Kim YM, Peters PJ, Ploegh H, In vivo control of endosomal architecture by class II-associated invariant chain and cathepsin S. EUR J IMMUNOL 2005;35 (9):2552-2562 [PubMed]
  • Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S, Quantitative and dynamic assessment of the contribution of the ER to phagosome formation. CELL 2005;123 (1):157-170 [PubMed]
  • van der Wel NN, Fluitsma DM, Dascher CC, Brenner MB, Peters PJ, Subcellular localization of mycobacteria in tissues and detection of lipid antigens in organelles using cryo-techniques for light and electron microscopy. CURR OPIN MICROBIOL 2005;8 (3):323-330 [PubMed]


  • Baas AF, Kuipers J, van der Wel NN, Batlle E, Koerten HK, Peters PJ, Clevers HC, Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD. CELL 2004;116 (3):457-466 [PubMed]
  • Griparic L, van der Wel NN, Orozco IJ, Peters PJ, van der Bliek AM, Loss of the intermembrane space protein Mgm1/OPA1 induces swelling and localized constrictions along the lengths of mitochondria. J BIOL CHEM 2004;279 (18):18792-18798 [PubMed]


  • Bertens P, Heijne W, van der Wel N, Wellink J, van Kammen A, Studies on the C-terminus of the Cowpea mosaic virus movement protein. ARCH VIROL 2003;148 (2):265-279 [PubMed]
  • Cernadas M, Sugita M, van der Wel N, Cao X, Gumperz JE, Maltsev S, Besra GS, Behar SM, Peters PJ, Brenner MB, Lysosomal localization of murine CD1d mediated by AP-3 is necessary for NK T cell development. J IMMUNOL 2003;171 (8):4149-4155 [PubMed]
  • Puertollano R, van der Wel NN, Greene LE, Eisenberg E, Peters PJ, Bonifacino JS, Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network. MOL BIOL CELL 2003;14 (4):1545-1557 [PubMed]
  • van der Wel NN, Sugita M, Fluitsma DM, Cao X, Schreibelt G, Brenner MB, Peters PJ, CD1 and major histocompatibility complex II molecules follow a different course during dendritic cell maturation. MOL BIOL CELL 2003;14 (8):3378-3388 [PubMed]


  • Cao X, Sugita M, van der Wel N, Lai J, Rogers RA, Peters PJ, Brenner MB, CD1 molecules efficiently present antigen in immature dendritic cells and traffic independently of MHC class II during dendritic cell maturation. J IMMUNOL 2002;169 (9):4770-4777 [PubMed]


  • Sugita M, van der Wel N, Rogers RA, Peters PJ, Brenner MB, CD1c molecules broadly survey the endocytic system. P NATL ACAD SCI USA 2000;97 (15):8445-8450 [PubMed]


  • van der Wel NN, Goldbach RW, van Lent JW, The movement protein and coat protein of alfalfa mosaic virus accumulate in structurally modified plasmodesmata. VIROLOGY 1998;244 (2):322-329 [PubMed]


  • Kasteel DT, van der Wel NN, Jansen KA, Goldbach RW, van Lent JW, Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus. J GEN VIROL 1997;78 (8):2089-2093 [PubMed]