R.A. Bem MD PhD publications

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Publications MD PhD R.A. Bem

Position
Medical Specialist
Main activities
Patient care, Research
Specialisation
Pediatrics, Intensive Care
Focus of research

My main focus of research is Acute Lung Disease and Injury in Children.

Within this theme I have special interest in acute, severe viral (respiratory syncytial virus, RSV) infections and the effects of (pediatric) intensive care unit treatments such as mechanical ventilation, hyperoxia and fluid therapy. Research studies include clinical studies, translational animal studies (mice, rats, lambs and calves) and laboratory in vitro studies using complex co-cultures with immune (eg, neutrophils) and airway epithelial cells. With this work, I have characterized pneumovirus animal models for severe RSV infections in the last years. Furthermore, I am unraveling the importance of neutrophil extracellular traps in the pathophysiology of airway mucus obstruction.

 

Within this theme I have successfully initiated and established collaboration with multiple (inter)national partners from the:

- National Institutes of Allergy and Infectious Diseases (NIAID, NIH), Bethesda, MD, USA

- Department of Pulmonary and Critical Care, University of Washington, Seattle, WA, USA

- Bioveterinary Research University of Wageningen, Lelystad, The Netherlands

- Department of Pediatric Infectious Diseases, Wilhelmina Children’s Hospital, Utrecht, The Netherlands

- Department of Pediatric Pulmonology, Children’s Hospital Richmond, VG, USA

- Department of Lung Development and Remodeling, Max Planck Institute, Bad Nauheim, Germany

 

Other established research lines include:

- Non-invasive ventilation face mask personalization by 3D scanning and printing techniques (in collaboration with Technical medicine from the TU Twente and Industrial Engineers from the TU Delft)

- Aerosols and SARS-CoV-2 transmission (in collaboration with Van der Waals-Zeeman Institute, Institute of Physics, University of Amsterdam)

 

 

Amsterdam UMC Research Institutes
AMC departments
All publications

2021

  • Linssen Rosalie S., Chai Guihong, Ma Jonathan, Kummarapurugu Apparao B., van Woensel Job B. M., Bem Reinout A., Kaler Logan, Duncan Gregg A., Zhou Lei, Rubin Bruce K., Xu Qingguo Neutrophil extracellular traps increase airway mucus viscoelasticity and slow mucus particle transit American journal of respiratory cell and molecular biology 2021;64 (1):69-78 [PubMed]

2020

  • Smith Scott H., Somsen G. Aernout, van Rijn Cees, Kooij Stefan, van der Hoek Lia, Bem Reinout A., Bonn Daniel Aerosol persistence in relation to possible transmission of SARS-CoV-2 Physics of Fluids 2020;32 (10) [PubMed]
  • Bem Reinout A. Inhaled Nitric Oxide in Pediatric Acute Hypoxic Respiratory Failure: No Time to Relax Pediatric critical care medicine 2020;21 (8):767-768 [PubMed]
  • de Groot Marcel G, de Neef Marjorie, Otten Marieke H, van Woensel Job B M, Bem Reinout A Interobserver Agreement on Clinical Judgment of Work of Breathing in Spontaneously Breathing Children in the Pediatric Intensive Care Unit Pediatric intensive care nursing 2020;9 (1):34-39 [PubMed]
  • Bem Reinout A. Is there a causal relationship between respiratory syncytial virus lower respiratory tract infection and chronic wheezing? lancet. Respiratory medicine 2020;8 (8):749-750
  • Bem Reinout A., Bont Louis J., van Woensel Job B. M. Life-threatening bronchiolitis in children: eight decades of critical care lancet. Respiratory medicine 2020;8 (2):142-144
  • Ingelse Sarah A., Pisani Luigi, Westdorp Marloes H. A., Almakdase Mariam, Schultz Marcus J., van Woensel Job B. M., Bem Reinout A. Lung ultrasound scoring in invasive mechanically ventilated children with severe bronchiolitis Pediatric pulmonology 2020;55 (10):2799-2805 [PubMed]
  • Somsen G. Aernout, van Rijn Cees J. M., Kooij Stefan, Bem Reinout A., Bonn Daniel Measurement of small droplet aerosol concentrations in public spaces using handheld particle counters Physics of Fluids 2020;32 (12) [PubMed]
  • Linssen R. S. N., Ma J., Bem R. A., Rubin B. K. Rational use of mucoactive medications to treat pediatric airway disease Paediatric respiratory reviews 2020;36:8-14 [PubMed]
  • van Rijn Cees, Somsen G. Aernout, Hofstra Leonard, Dahhan Ghassan, Bem Reinout A., Kooij Stefan, Bonn Daniel Reducing aerosol transmission of SARS-CoV-2 in hospital elevators Indoor air 2020;30 (6):1065-1066 [PubMed]
  • Somsen G. Aernout, van Rijn Cees, Kooij Stefan, Bem Reinout A., Bonn Daniel Small droplet aerosols in poorly ventilated spaces and SARS-CoV-2 transmission lancet. Respiratory medicine 2020;8 (7):658-659 [PubMed]
  • den Hollander Bibiche, Linssen Rosalie S. N., Cortjens Bart, van Etten-Jamaludin Fardi S., van Woensel Job B. M., Bem Reinout A. Use of dornase alfa in the paediatric intensive care unit: Current literature and a national cross-sectional survey European journal of hospital pharmacy 2020 [PubMed]

2019

  • Wiegers Hanke M. G., van Nijen Lisa, van Woensel Job B. M., Bem Reinout A., de Jong Menno D., Calis Job C. J. Bacterial co-infection of the respiratory tract in ventilated children with bronchiolitis; A retrospective cohort study BMC infectious diseases 2019;19 (1) [PubMed]
  • Ingelse Sarah A., Juschten Jenny, Maas Martinus A. W., Matute-Bello Gustavo, Juffermans Nicole P., van Woensel Job B. M., Bem Reinout A. Fluid restriction reduces pulmonary edema in a model of acute lung injury in mechanically ventilated rats PLoS ONE 2019;14 (1) [PubMed]
  • Cortjens B., de Jong R., Bonsing J. G., van Woensel J. B. M., Bem R. A., Antonis A. F. G. Human respiratory syncytial virus infection in the pre-clinical calf model Comparative Immunology, Microbiology and Infectious Diseases 2019;65:213-218 [PubMed]
  • Ingelse Sarah A., Geukers Vincent G., Dijsselhof Monique E., Lemson Joris, Bem Reinout A., van Woensel Job B. Less Is More?—A Feasibility Study of Fluid Strategy in Critically Ill Children With Acute Respiratory Tract Infection Frontiers in pediatrics 2019;7 [PubMed]
  • Bem Reinout A. Noninvasive High Flow Versus Noninvasive Positive Pressure in Children With Severe Bronchiolitis: The Battle of the PICU Continues Pediatric critical care medicine 2019;20 (2):192-193 [PubMed]
  • Bem Reinout A. The author replies Pediatric critical care medicine 2019;20 (8):798 [PubMed]

2018

  • Cortjens Bart, de Jong Rineke, Bonsing Judith G., van Woensel Job B. M., Antonis Adriaan F. G., Bem Reinout A. Local dornase alfa treatment reduces NETs-induced airway obstruction during severe RSV infection Thorax 2018;73 (6):578-580 [PubMed]
  • Vester M. E. M., van Rijn R. R., Bem R. A., Maes A., Kubat B. Subdural hematoma in an infant with alpha-1-antitrypsin deficiency and a rare primary intra-osseous vascular malformation Journal of Forensic Radiology and Imaging 2018;13:23-26

2017

  • Ingelse Sarah A., Wiegers Hanke M. G., Calis Job C., van Woensel Job B., Bem Reinout A. Early Fluid Overload Prolongs Mechanical Ventilation in Children With Viral-Lower Respiratory Tract Disease Pediatric critical care medicine 2017;18 (3):e106-e111 [PubMed]
  • Cortjens B., van Woensel J. B. M., Bem R. A. Neutrophil Extracellular Traps in Respiratory Disease: guided anti-microbial traps or toxic webs? Paediatric respiratory reviews 2017;21:54-61 [PubMed]
  • Cortjens Bart Neutrophils in respiratory syncytial virus disease: Untangling the NET 2017. 191p. [UvA Dissertations Online] University of Amsterdam. (Supervisor: van Woensel J. B. M.; Co-supervisor: Bem R. A.)
  • Cortjens Bart, Ingelse Sarah A., Calis Job C., Vlaar Alexander P., Koenderman Leo, Bem Reinout A., van Woensel Job B. Neutrophil subset responses in infants with severe viral respiratory infection Clinical immunology (Orlando, Fla.) 2017;176:100-106 [PubMed]
  • Bem Reinout A., Kneyber Martin C., van Woensel Job B. M. Respiratory syncytial virus-induced paediatric ARDS: why we should unpack the syndrome lancet. Respiratory medicine 2017;5 (1):9-10 [PubMed]

2016

  • Cortjens Bart, de Boer Onno J., de Jong Rineke, Antonis Adriaan Fg, Sabogal Piñeros Yanaika S., Lutter René, van Woensel Job Bm, Bem Reinout A. Neutrophil extracellular traps cause airway obstruction during respiratory syncytial virus disease Journal of pathology 2016;238 (3):401-411 [PubMed]
  • Ingelse Sarah A., Wösten-van Asperen Roelie M., Lemson Joris, Daams Joost G., Bem Reinout A., van Woensel Job B. Pediatric Acute Respiratory Distress Syndrome: Fluid Management in the PICU Frontiers in pediatrics 2016;4:UNSP 21 [PubMed]
  • Cortjens Bart, Lutter René, Boon Louis, Bem Reinout A., van Woensel Job B. M. Pneumovirus-Induced Lung Disease in Mice Is Independent of Neutrophil-Driven Inflammation PLoS ONE 2016;11 (12):e0168779 [PubMed]

2015

  • van den Berg Elske Mechanisms and consequences of lung epithelial injury in severe RSV disease 2015. 151p. ISBN 9789462597334. [UvA Dissertations Online] University of Amsterdam. (Supervisor: Bos A. P.; Co-supervisors: van Woensel J. B. M., Bem R. A.)
  • van den Berg Elske, Bal Suzanne M., Kuipers Maria T., Matute-Bello Gustavo, Lutter René, Bos Albert P., van Woensel Job B. M., Bem Reinout A. The caspase inhibitor zVAD increases lung inflammation in pneumovirus infection in mice Physiological reports 2015;3 (3):e12332 [PubMed]

2014

  • van den Berg Elske, Bem Reinout A., Bos Albert P., Lutter Rene, van Woensel Job B. M. The effect of TIP on pneumovirus-induced pulmonary edema in mice PLoS ONE 2014;9 (7):e102749 [PubMed]

2013

  • van den Berg Elske, van Woensel Job B. M., Bem Reinout A. Apoptosis in pneumovirus infection Viruses 2013;5 (1):406-422 [PubMed]
  • Bem Reinout A., van den Berg Elske, Suidgeest Ernst, van der Weerd Louise, van Woensel Job B. M., Grotenhuis Heynric B. Cardiac dysfunction in pneumovirus-induced lung injury in mice Pediatric critical care medicine 2013;14 (5):e243-e249 [PubMed]
  • Wösten-van Asperen Roelie M., Bos Albert P., Bem Reinout A., Dierdorp Barbara S., Dekker Tamara, van Goor Harry, Kamilic Jelena, van der Loos Chris M., van den Berg Elske, Bruijn Martijn, van Woensel Job B., Lutter René Imbalance between pulmonary angiotensin-converting enzyme and angiotensin-converting enzyme 2 activity in acute respiratory distress syndrome Pediatric critical care medicine 2013;14 (9):e438-e441 [PubMed]

2011

  • Bem Reinout A., Domachowske Joseph B., Rosenberg Helene F. Animal models of human respiratory syncytial virus disease American journal of physiology. Lung cellular and molecular physiology 2011;301 (2):L148-L156 [PubMed]
  • van den Berg Elske, van Woensel Job B. M., Bos Albert P., Bem Reinout A., Altemeier William A., Gill Sean E., Martin Thomas R., Matute-Bello Gustavo Role of the Fas/FasL system in a model of RSV infection in mechanically ventilated mice American journal of physiology. Lung cellular and molecular physiology 2011;301 (4):L451-L460 [PubMed]

2010

  • Bem R. A. Acute lung injury in children: from viral infection and mechanical ventilation to inflammation and apoptosis 2010. 175p. [UvA Dissertations Online] University of Amsterdam. (Supervisor: Heymans H. S. A.; Co-supervisors: Bos A. P., van Woensel J. B. M.)
  • Bruijn Martijn, Bem Reinout A., Bos Albert P., van Woensel Job B. M. Acute lung injury in children: Importance of host factors Critical care medicine 2010;38 (1):350 [PubMed]
  • Bem Reinout A., van der Loos Chris M., van Woensel Job B. M., Bos Albert P. Cleaved caspase-3 in lung epithelium of children who died with acute respiratory distress syndrome Pediatric critical care medicine 2010;11 (5):556-560 [PubMed]
  • Bem Reinout A., van Woensel Job B. M., Lutter Rene, Domachowske Joseph B., Medema Jan Paul, Rosenberg Helene F., Bos Albert P. Granzyme A- and B-Cluster Deficiency Delays Acute Lung Injury in Pneumovirus-Infected Mice Journal of immunology (Baltimore, Md. 2010;184 (2):931-938 [PubMed]
  • Bem Reinout A., Bos Albert P., Wösten-van Asperen Roelie M., Bruijn Martijn, Lutter Rene, Sprick Martin R., van Woensel Job B. M. Potential Role of Soluble TRAIL in Epithelial Injury in Children with Severe RSV Infection American journal of respiratory cell and molecular biology 2010;42 (6):697-705 [PubMed]

2009

  • Bem Reinout Alexander, van Woensel Job B. M., Bos Albert P., Koski Amy, Farnand Alex W., Domachowske Joseph B., Rosenberg Helene F., Martin Thomas R., Matute-Bello Gustavo Mechanical ventilation enhances lung inflammation and caspase activity in a model of mouse pneumovirus infection American journal of physiology. Lung cellular and molecular physiology 2009;296 (1):L46-L56 [PubMed]

2008

  • Bem Reinout A., Bos Albert P., Bots Michael, Wolbink Angela M., van Ham S. Marieke, Medema Jan Paul, Lutter Rene, van Woensel Job B. M. Activation of the granzyme pathway in children with severe respiratory syncytial virus infection Pediatric research 2008;63 (6):650-655 [PubMed]
  • Bem R. A., Farnand A. W., Wong V., Koski A., Rosenfeld M. E., van Rooijen N., Frevert C. W., Martin T. R., Matute-Bello G. Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice American journal of physiology. Lung cellular and molecular physiology 2008;295 (2):L314-L325 [PubMed]

2007

  • Bem Reinout A., Bos Albert P., Matute-Bello Gustavo, van Tuyl Minke, van Woensel Job B. M. Lung epithelial cell apoptosis during acute lung injury in infancy Pediatric critical care medicine 2007;8 (2):132-137 [PubMed]
  • Bem Reinout A., Bos Albert P., van Woensel Job B. M. The regulation of respiratory epithelial cell apoptosis by nitric oxide in acute lung injury - Reply Pediatric critical care medicine 2007;8 (4):410
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