Prof. S.S. Zeerleder MD PhD publications

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Publications Prof. MD PhD S.S. Zeerleder

Position
Full Professor
Main activities
Patient care, Research
Specialisation
Internist-hematologist
Focus of research

Upon cell damage and cell death molecular structures, so-called damage-associated molecular pattern (DAMPs) are released into the circulation and induce a systemic inflammatory response by ligation of pattern recognition receptors (PRR). DAMPs released from nucleated cells include- among others- extra-cellular (cell-free) DNA, DNA-binding proteins (e.g. Histones, HMGB-1) and heme. DAMPs released from erythrocytes comprise hemoglobin, iron and heme, respectively. Extracellular DNA, e.g nucleosomes, and HMGB1 have been demonstrated to induce an inflammatory response via Toll-like receptor (TLR) 9 and histones via TLR2 and TLR4, respectively. Heme is cytotoxic through the formation of highly active reactive oxygen species (ROS), an effect that is - in part - dependent on TLR4 and induction of the NFkappaB pathway. Complement activation upon damage of nucleated and non-nucleated cells propagate the inflammatory response induced by DAMPs and may lead by itself to cell destruction.

DAMPs may have detrimental effects by inducing a systemic inflammation resulting in fatality. In addition, the systemic inflammation may also facilitate and perpetuate (uncontrolled) activation of the adaptive immune response. Therefore the organism has multiple “self-defense” strategies to neutralize the potential fatal effects of DAMPs. These strategies include a tight regulation of DAMP release and the presence of systemic inhibitors (e.g. plasma proteins) to neutralize the toxic effects of DAMPs. The last years it became evident that DAMPs may play significant role in the inflammatory processes occurring in hematological malignancies, e.g. graft versus host disease (GvHD) and neutropenic fever, as well as in benign hematological diseases, such as sickle cell disease, paroxysomal nocturnal hemoglobinuria (PNH) and autoimmune hemolytic anemia (AIHA). The research of my group focuses on the mechanism on how DAMPs are released, on the regulation of this process with a special focus on the role of plasma proteins herein, the identification of the cellular source of the DAMPs, and the systemic biological effects of the released DAMPs.

 

Focus 1: mechanism, regulation and biological effects of DAMP-release from nucleated cells

Given the highly conserved structure of DAMPs and the cross-reactivity of antibodies reacting with DAMPs reliable systemic measurement of DAMPs is troublesome. We set up a couple of assays to measure DAMPs in plasma, e.g in the form of nucleosomes or using RT-PCR detecting cell-specific DNA. Combined measurement of neutrophilic proteases and nucleosomes turned out to be reliable measures in plasma for neutrophil activation in the form of neutrophil extracellular traps (NETs). Others and we showed NET formation to be involved in the pathogenesis in various diseases (e.g. sepsis, typhoid fever, melioidosis, sickle cell disease). We also showed NET formation to contribute to venous thrombosis. DAMP release from nucleated dead cells is highly regulated. We identified Factor VII-activating protease (FSAP), a plasmatic serine protease which is activated upon contact with dead cells, to “detoxify” apoptotic cells by removing the nuclear contents in form of nucleosomes and – in concert- with DNAse1 also from necrotic cells. We identified histone 1 (H1) to be the target in necrotic cells, which is cleaved by FSAP resulting in nucleosome release. We showed that plasma protein inhibitors, such as C1-inhibitor, α2-antiplasmin, plasminogen activator inhibitor (PAI)-1 and tissue factor pathway inhibitor (TFPI) inhibit FSAP activation and activity and hence the release of nucleosomes. We unraveled the mechanism on how TFPI precisely binds and inhibits FSAP activity. We recently demonstrated FSAP to be activated by core histones (H2A, H2B, H3 and H4) as well and subsequently to cleave core histones resulting in neutralization of histone cytotoxicity. Together these results suggest that FSAP protects the host form cytotoxic effects of histones released during inflammation.

 

Focus 2: DAMPs in hemolytic diseases Hemolytic diseases are characterized by a shortened survival of erythrocytes in the circulation due to intra- and/or –extravascular hemolysis. Upon hemolysis of erythrocytes, DAMPs (e.g. free hemoglobin and heme) are released into the circulation resulting in systemic inflammation. We study the mechanism on how erythrocytes are removed from and/or lysed in circulation with a special focus on the role of the complement system herein. We show that complement mediated hemolysis of erythrocytes occurs in a significant percentage of patients with autoimmune hemolytic anemia (AIHA), which is mainly IgM driven. To date we are characterizing these antibodies with regard to their glycosylation pattern, rearrangement and isotype structure (penta- vs hexameric forms). We demonstrated that complement inhibiting strategies, e.g. plasma-derived C1-inhibitor or inhibitors of C3 can efficiently block complement mediated erythrocyte destruction in AHIA patients. At the moment we are performing an investigator-initiated clinical phase 1 trial to test the efficacy of C1-inhibitor in complement-mediated destruction of erythrocytes in patients suffering from AIHA. We also study the systemic consequences of erythrocyte lysis. The release of heme induces a strong systemic inflammatory response among others by the activation of neutrophils through the formation of NETs. We demonstrated that neutrophil activation in form of NET is significantly involved in the pathogenesis of organ damage in sickle cell disease and is surprisingly driven by iron rather than heme. Interestingly, preliminary results from our group suggest that NET formation is also involved in the pathogenesis of AIHA. We actually study the efficacy of plasma proteins hemopexin, haptoglobin and apotransferrin to neutralize the proinflammatory effects of heme and iron, respectively. In addition we study the role of inducible heme-oxygenase-1 (HO-1) in systemic inflammation induced by infection and intravascular hemolysis.

Focus 3: Therapeutic plasma proteins to neutralize systemic effects of DAMPs

We have a solid track record in protein purification and structure-function analysis. During the last 15 years we purified many plasma proteins devoid of significant impurities, such as FSAP, C1-inhibitor, TFPI, hemopexin, haptoglobin and α1-chymotrypsin. We optimized protocols in order to facilitate up- scaling of purification processes for these proteins from plasma as well as from waste fractions of Cohn fractionation in order to produce therapeutic proteins on large scale. We generated monoclonal- and polyclonal antibodies to these proteins in order to setup assays to measure protein concentration, activity and protein variants. We also explored the production of recombinant proteins with potential therapeutic indications using techniques to select proteins with optimal glycosylation resulting in comparable biological half-life to the plasma-derived product as well as screening techniques selecting specific glycol-variants of the respective proteins.

Diseases of interest: AIHA, PNH, sickle cell disease, acute graft-vs host disease, transfusion complications, sepsis

Technologies
ELISA, protein purification, protein structure-function analysis, production of mono- and polyclonal antibodies, gel electrophoresis, western blot, molecular biology techniques, mouse models for inflammation, GvHD and transfusion; rat models for transfusion

Amsterdam UMC Research Institutes
AMC departments
All publications

2021

  • Zeerleder Sacha, Engel Ruchira, Zhang Tao, Roem Dorina, van Mierlo Gerard, Wagenaar‐bos Ineke, van Ham Sija Marieke, Wuhrer Manfred, Wouters Diana, Jongerius Ilse Sugar matters: Improving in vivo clearance rate of highly glycosylated recombinant plasma proteins for therapeutic use Pharmaceuticals 2021;14 (1):1-13 [PubMed]

2020

  • van Lier Yannouck F., de Bree Godelieve J., Jonkers René E., Roelofs Joris J. T. H., ten Berge Ineke J. M., Rutten Caroline E., Nur Erfan, Kuijpers Taco W., Hazenberg Mette D., Zeerleder Sacha S. Allogeneic hematopoietic cell transplantation in the management of GATA2 deficiency and pulmonary alveolar proteinosis Clinical immunology (Orlando, Fla.) 2020;218 [PubMed]
  • Pabst Thomas, Joncourt Raphael, Shumilov Evgenii, Heini Alexander, Wiedemann Gertrud, Legros Myriam, Seipel Katja, Schild Christof, Jalowiec Katarzyna, Mansouri Taleghani Behrouz, Fux Michaela, Novak Urban, Porret Naomi, Zeerleder Sacha, Bacher Ulrike Analysis of IL-6 serum levels and CAR T cell-specific digital PCR in the context of cytokine release syndrome Experimental hematology 2020;88:7-14.e3 [PubMed]
  • Baas Inge, Delvasto-Nuñez Laura, Ligthart Peter, Brouwer Conny, Folman Claudia, Reis Edimara S., Ricklin Daniel, Lambris John D., Wouters Diana, de Haas Masja, Jongerius Ilse, Zeerleder Sacha S. Complement C3 inhibition by compstatin Cp40 prevents intra- and extravascular hemolysis of red blood cells Haematologica 2020;105 (2):e57-e60 [PubMed]
  • van Lier Yannouck F., Davids Mark, Haverkate Nienke J. E., de Groot Pieter F., Donker Marjolein L., Meijer Ellen, Heubel-Moenen Floor C. J. I., Nur Erfan, Zeerleder Sacha S., Nieuwdorp Max, Blom Bianca, Hazenberg Mette D. Donor fecal microbiota transplantation ameliorates intestinal graft-versus-host disease in allogeneic hematopoietic cell transplant recipients Science translational medicine 2020;12 (556) [PubMed]
  • Yang Jack, van Engelen Tjitske S. R., Haak Bastiaan W., Bonta Peter I., Majoor Christof J., van 't Veer Cornelis, de Vos Alex F., Kemper E. Marleen, Lutter René, van Mierlo Gerard, Zeerleder Sacha S., Bel Elisabeth H., van der Poll Tom Effect of C1-inhibitor in adults with mild asthma: A randomized controlled trial Allergy 2020;75 (4):953-955 [PubMed]
  • van de Geer Annemarie Neutrophil defects and deficiencies 2020. 238p. ISBN 9789463614740. [UvA Dissertations Online] University of Amsterdam. (Supervisors: Kuijpers Taco W., Zeerleder Sacha S.)
  • de Beer Friso M, Wieske Luuk, van Mierlo Gerard, Wouters Diana, Zeerleder Sacha, Bos Lieuwe D, Juffermans Nicole P, Schultz Marcus J, van der Poll Tom, Lagrand Wim K, Horn Janneke The effects of tidal volume size and driving pressure levels on pulmonary complement activation: an observational study in critically ill patients Intensive Care Medicine Experimental 2020;8 (Suppl 1):74 [PubMed]
  • D'Alessandro Elisa, Becker Christian, Bergmeier Wolfgang, Bode Christoph, Bourne Joshua H., Brown Helena, Buller Harry R., ten Cate-Hoek Arina J., ten Cate Vincent, van Cauteren Yvonne J. M., Cheung Yam F. H., Cleuren Audrey, Coenen Danielle, Crijns Harry J. G. M., de Simone Ilaria, Dolleman Sophie C., Klein Christine Espinola, Fernandez Delia I., Granneman Lianne, van t Hof Arnoud, Henke Peter, Henskens Yvonne M. C., Huang Jingnan, Jennings Lisa K., Jooss Natalie, Karel Mieke, van den Kerkhof Danique, Klok Frederik A., Kremers Bram, Lämmle Bernhard, Leader Avi, Lundstrom Annika, Mackman Nigel, Mannucci Pier M., Maqsood Zahra, van der Meijden Paola E. J., van Moorsel Marc, Moran Luis A., Morser John, van Mourik Manouk, Navarro Stefano, Neagoe Raluca A. I., Olie Renske H., van Paridon Pauline, Posma Jens, Provenzale Isabella, Reitsma Pieter H., Scaf Billy, Schurgers Leon, Seelig Jaap, Siegbahn Agneta, Siegerink Bob, Soehnlein Oliver, Soriano Eva Maria, Sowa Marcin A., Spronk Henri M. H., Storey Robert F., Tantiwong Chukiat, Veninga Alicia, Wang Xueqing, Watson Steve P., Weitz Jeff, Zeerleder Sacha S., ten Cate Hugo, Lina Badimon, Christoph Binder, Marc Hoylaerts, Peter Karlheinz, Rory Koenen, Gregory Lip, Steffen Massberg, Philipp Von Hundelshausen, Christian Weber, Johann Wojta Thrombo-Inflammation in Cardiovascular Disease: An Expert Consensus Document from the Third Maastricht Consensus Conference on Thrombosis Thrombosis and haemostasis 2020;120 (4):538-564 [PubMed]

2019

  • Cohn Danny M., Zeerleder Sacha S., Meijers Joost C. M., Stroes Erik S. G., Levi Marcel Albumin plasma exchange for life-threatening angioedema with normal C1-inhibitor Journal of Allergy and Clinical Immunology: In Practice 2019;7 (4):1360-1361 [PubMed]
  • Rijkers Maaike, Schmidt David, Lu Nina, Kramer Cynthia S M, Heidt Sebastiaan, Mulder Arend, Porcelijn Leendert, Claas Frans H J, Leebeek Frank W G, Jansen A J Gerard, Jongerius Ilse, Zeerleder Sacha S, Vidarsson Gestur, Voorberg Jan, de Haas Masja Anti-HLA antibodies with complementary and synergistic interaction geometries promote classical complement activation on platelets Haematologica 2019;104 (2):403-416 [PubMed]
  • Jäger Ulrich, Barcellini Wilma, Broome Catherine M., Gertz Morie A., Hill Anita, Hill Quentin A., Jilma Bernd, Kuter David J., Michel Marc, Montillo Marco, Röth Alexander, Zeerleder Sacha S., Berentsen Sigbjørn Diagnosis and treatment of autoimmune hemolytic anemia in adults: Recommendations from the First International Consensus Meeting Blood reviews 2019 [PubMed]
  • Kalin Burak, ter Borg Mariëtte, Wijers Rebecca, Somers Judith A. E., van der Holt Bronno, van Bergen Cornelis A. M., Petersen Eefke J., Kuball Jurgen, Meijer Ellen, Schaap Nicolaas P. M., Zeerleder Sacha S., Broers Annoek E., Braakman Eric, Frederik Falkenburg J. H., Lamers Cor H. J., Cornelissen Jan J. Double Umbilical Cord Blood Transplantation in High-Risk Hematological Patients: A Phase II Study Focusing on the Mechanism of Graft Predominance HemaSphere 2019;3 (5) [PubMed]
  • Garcia Garrido Hannah M, van Aalst Mariëlle, Schinkel Janke, Koen Gerrit, Defoer Jacqueline M, Hazenberg Mette D, Nur Erfan, Grobusch Martin P, Zeerleder Sascha S, Goorhuis Abraham, de Bree Godelieve J Early loss of immunity against measles following allogeneic hematopoietic stem cell transplantation American journal of hematology 2019;94 (10):E270-E272 [PubMed]
  • Geneugelijk Kirsten, Thus Kirsten A., van Deutekom Hanneke W. M., Calis Jorg J. A., Borst Eric, Keşmir Can, Oudshoorn Machteld, van der Holt Bronno, Meijer Ellen, Zeerleder Sacha, de Groot Marco R., von dem Borne Peter A., Schaap Nicolaas, Cornelissen Jan, Kuball J. rgen, Spierings Eric Exploratory Study of Predicted Indirectly ReCognizable HLA Epitopes in Mismatched Hematopoietic Cell Transplantations Frontiers in immunology 2019;10:880 [PubMed]
  • Levi Marcel, Cohn Danny M, Zeerleder Sacha Hereditary angioedema: Linking complement regulation to the coagulation system Research and practice in thrombosis and haemostasis 2019;3 (1):38-43 [PubMed]
  • Levi Marcel, Cohn Danny, Zeerleder Sacha, Dziadzio Magdalena, Longhurst Hilary Long-term effects upon rituximab treatment of acquired angioedema due to C1-inhibitor deficiency Allergy 2019;74 (4):834-840 [PubMed]
  • Langedijk Annefleur C., van Aalst Mariëlle, Meek Bob, van Leeuwen Ester M. M., Zeerleder Sacha, Meijer Ellen, Hazenberg Mette D., Grobusch Martin P., Goorhuis Abraham Long-term pneumococcal vaccine immunogenicity following allogeneic hematopoietic stem cell transplantation Vaccine 2019;37 (3):510-515 [PubMed]
  • van Avondt Kristof, Nur Erfan, Zeerleder Sacha Mechanisms of haemolysis-induced kidney injury Nature reviews. Nephrology 2019;15 (11):671-692 [PubMed]
  • Heestermans Marco, Salloum-Asfar Salam, Streef Tom, Laghmani El Houari, Salvatori Daniela, Luken Brenda M., Zeerleder Sacha S., Spronk Henri M. H., Korporaal Suzanne J., Kirchhofer Daniel, Wagenaar Gerry T. M., Versteeg Henri H., Reitsma Pieter H., Renné Thomas, van Vlijmen Bart J. M. Mouse venous thrombosis upon silencing of anticoagulants depends on tissue factor and platelets, not FXII or neutrophils Blood 2019;133 (19):2090-2099 [PubMed]
  • van der Meer Anne Jan, Ding Chao, Hoogendijk Arie Johan, de Vos Alex F., Boon Louis, Zeerleder Sacha S., van der Poll Tom Neutrophils mitigate the systemic host response during endotoxemia in mice Immunology 2019;156 (3):277-281 [PubMed]
  • Thielen Astrid J. F. Regulation of complement activation on red blood cells 2019. 161p. ISBN 9789463264504. [UvA Dissertations Online] University of Amsterdam. (Supervisors: Zeerleder S. S., van Ham S. M., Wouters D.)
  • Kremers Bram M. M., Birocchi Simone, van Oerle Rene, Zeerleder Sacha, Spronk Henri M. H., Mees Barend M. E., Luken Brenda M., ten Cate Hugo, ten Cate-Hoek Arina J. Searching for a Common Thrombo-Inflammatory Basis in Patients With Deep Vein Thrombosis or Peripheral Artery Disease Frontiers in cardiovascular medicine 2019;6 [PubMed]
  • de Jong Greta, Janssen Jeroen J. W. M., Biemond Bart J., Zeerleder Sacha S., Ossenkoppele Gert J., Visser Otto, Nur Erfan, Meijer Ellen, Hazenberg Mette D. Survival of early posthematopoietic stem cell transplantation relapse of myeloid malignancies European journal of haematology 2019;103 (5):491-499 [PubMed]
  • van der Meer Anne Jan, Kroeze Anna, Hoogendijk Arie J., Soussan Aicha Ait, Ellen van der Schoot C., Wuillemin Walter A., Voermans Carlijn, van der Poll Tom, Zeerleder Sacha Systemic inflammation induces release of cell-free DNA from hematopoietic and parenchymal cells in mice and humans Blood advances 2019;3 (5):724-728 [PubMed]
  • van Golen Rowan F., Reiniers Megan J., Marsman Gerben, Alles Lindy K., van Rooyen Derrick M., Petri Björn, van der Mark Vincent A., van Beek Adriaan A., Meijer Ben, Maas Martinus A., Zeerleder Sacha, Verheij Joanne, Farrell Geoffrey C., Luken Brenda M., Teoh Narci C., van Gulik Thomas M., Murphy Michael P., Heger Michal The damage-associated molecular pattern HMGB1 is released early after clinical hepatic ischemia/reperfusion BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 2019;1865 (6):1192-1200 [PubMed]
  • Schuster Stephen J., Bishop Michael R., Tam Constantine S., Waller Edmund K., Borchmann Peter, McGuirk Joseph P., Jäger Ulrich, Jaglowski Samantha, Andreadis Charalambos, Westin Jason R., Fleury Isabelle, Bachanova Veronika, Foley S. Ronan, Ho P. Joy, Mielke Stephan, Magenau John M., Holte Harald, Pantano Serafino, Pacaud Lida B., Awasthi Rakesh, Chu Jufen, Anak Özlem, Salles Gilles, Maziarz Richard T., Study group members AMC , Kersten Marie José J., Biemond Bart J., Burger Patrick, Claessen Marie-José J. A. G., Hazenberg Mette D., Kater Arnon P., Kemper E. M., Nur Erfan, Spiering Marjolein, Tonino Sanne H., Voermans Carlijn, Zeerleder Sacha S. Tisagenlecleucel in adult relapsed or refractory diffuse large B-cell lymphoma New England journal of medicine 2019;380 (1):45-56 [PubMed]

2018

  • Vlijm-Kievit Anniek, Jorna Nori G. E., Moll Etelka, Pajkrt Eva, Pals Steven T., Middeldorp Saskia, Biemond Bart J., Zeerleder Sacha S., Tio Marieke A., Kemper E. Marleen, Hazenberg Mette D. Acute lymphoblastic leukemia during the third trimester of pregnancy Leukemia & lymphoma 2018;59 (5):1274-1276 [PubMed]
  • Thielen Astrid J. F., Meulenbroek Elisabeth M., Baas Inge, Bruggen Robin, Zeerleder Sacha S., Wouters Diana Complement Deposition and IgG Binding on Stored Red Blood Cells Are Independent of Storage Time Transfusion medicine and hemotherapy 2018;45:378-384 [PubMed]
  • Thielen Astrid J. F., Zeerleder Sacha, Wouters Diana Consequences of dysregulated complement regulators on red blood cells Blood reviews 2018;32 (4):280-288 [PubMed]
  • Thielen Astrid J. F., van Baarsen Iris M., Jongsma Marlieke L., Zeerleder Sacha, Spaapen Robbert M., Wouters Diana CRISPR/Cas9 generated human CD46, CD55 and CD59 knockout cell lines as a tool for complement research Journal of immunological methods 2018;456:15-22 [PubMed]
  • Heestermans M., Salloum-Asfar S., Salvatori D., Laghmani E. H., Luken B. M., Zeerleder S. S., Spronk H. M. H., Korporaal S. J., Wagenaar G. T. M., Reitsma P. H., van Vlijmen B. J. M. Erratum: Role of platelets, neutrophils, and factor XII in spontaneous venous thrombosis in mice (Blood. (2016) 127: 21 (2630-2637) DOI: 10.1182/blood-2015-10-672766) Blood 2018;131 (26):2996 [PubMed]
  • Zeerleder Sacha Factor VII-Activating Protease: Hemostatic Protein or Immune Regulator? Seminars in thrombosis and hemostasis 2018;44 (2):151-158 [PubMed]
  • Korte Wolfgang, Zeerleder Sacha S. Hemotherapy with New Blood Products Transfusion medicine and hemotherapy 2018;45 (2):84 [PubMed]
  • Stroo Ingrid, Ding Chao, Novak Andreja, Yang Jack, Roelofs Joris J. T. H., Meijers Joost C. M., Revenko Alexey S., van ’t Veer Cornelis, Zeerleder Sacha, Crosby Jeff R., van der Poll Tom Inhibition of the extrinsic or intrinsic coagulation pathway during pneumonia-derived sepsis American journal of physiology. Lung cellular and molecular physiology 2018;315 (5):L799-L809 [PubMed]
  • Stavenhagen Kathrin, Kayili H. Mehmet, Holst Stephanie, Koeleman Carolien, Engel Ruchira, Wouters Diana, Zeerleder Sacha, Salih Bekir, Wuhrer Manfred N- and O-glycosylation analysis of human C1-inhibitor reveals extensive mucin-type O-glycosylation Molecular & cellular proteomics 2018;17 (6):1225-1238 [PubMed]
  • Popescu Narcis I., Silasi Robert, Keshari Ravi S., Girton Alanson, Burgett Tarea, Zeerleder Sacha S., Gailani David, Gruber Andras, Lupu Florea, Mark Coggeshall K. Peptidoglycan induces disseminated intravascular coagulation in baboons through activation of both coagulation pathways Blood 2018;132 (8):849-860 [PubMed]
  • van de Weerdt Emma K., Biemond Bart J., Zeerleder Sacha S., van Lienden Krijn P., Binnekade Jan M., Vlaar Alexander P. J., van Leent A., Koeman M., Ypma P. F., Arbous M. S., Demandt A. M. P., van Mook W. N. K. A., Tordoir J. H. M., Wolthuis E., Blomjous J. G. A. M., Determann R. M., Endeman H., Kerver E. D., van der Velden W. J. F. M., Vink R., Bokkers R. P. H., Mäkelburg A. B. U., van den Bergh W. M., Tuinman P. R., van Leent A., Koeman M., Ypma P. F., Arbous M. S., Demandt A. M. P., van Mook W. N. K. A., Tordoir J. H. M., Wolthuis E., Blomjous J. G. A. M., Determann R. M., Endeman H., Kerver E. D., van der Velden W. J. F. M., Vink R., Bokkers R. P. H., Mäkelburg A. B. U., van den Bergh W. M., Tuinman P. R. Prophylactic platelet transfusion prior to central venous catheter placement in patients with thrombocytopenia: Study protocol for a randomised controlled trial Trials 2018;19 (1) [PubMed]
  • Peters Anna L., van de Weerdt Emma K., Goudswaard Eline J., Binnekade Jan M., Zwaginga Jaap J., Beckers Erik A. M., Zeerleder Sacha S., van Kraaij Marian G. J., Juffermans Nicole P., Vlaar Alexander P. J. Reporting transfusion-related acute lung injury by clinical and preclinical disciplines Blood transfusion 2018;16 (3):227-234 [PubMed]
  • Tjon Jennifer M.-L., de Groot Marco R., Sypkens Smit Saskia M. A., de Wreede Liesbeth C., Snijders Tjeerd J. F., Koene Harry R., Meijer Ellen, Raaijmakers Marc H. G., Schaap Michel, Raymakers Reinier, Zeerleder Sacha S., Halkes Constantijn J. M. Short-term efficacy and safety of antithymocyte globulin treatment in elderly patients with acquired aplastic anaemia British journal of haematology 2018;180 (3):459-462 [PubMed]
  • Bosboom J. J., Klanderman R. B., Peters A. L., van de Weerdt E. K., Goudswaard E. J., Binnekade J. M., Zwaginga J. J., Beckers E. A. M., Geerts B. F., Hollmann M. W., Zeerleder S. S., van Kraaij M., Vlaar A. P. The practice of diagnosing and reporting transfusion-associated circulatory overload: a national survey among physicians and haemovigilance officers Transfusion medicine (Oxford, England) 2018;28 (5):363-370 [PubMed]
  • Engel Ruchira, Delvasto-Nuñez Laura, Roem Dorina, van Mierlo Gerard, Holst Stephanie, Hipgrave Ederveen Agnes L., van Buul Jaap D., Wuhrer Manfred, Wouters Diana, Zeerleder Sacha α1-Antichymotrypsin Present in Therapeutic C1-Inhibitor Products Competes with Selectin-Sialyl Lewis X Interaction Thrombosis and haemostasis 2018;118 (12):2134-2144 [PubMed]

2017

  • Emmens Reindert W., Oedayrajsingh-Varma Maikel, Woudstra Linde, Kamp Otto, Meinster Elisa, van Dijk Annemieke, Helder Marco N., Wouters Diana, Zeerleder Sacha, van Ham S. Marieke, de Jong Nico, Niessen Hans W. M., Juffermans Lynda J. M., Krijnen Paul A. J. A comparison in therapeutic efficacy of several time points of intravenous StemBell administration in a rat model of acute myocardial infarction Cytotherapy 2017;19 (1):131-140 [PubMed]
  • van de Geer A., Gazendam R. P., Tool A. T. J., van Hamme J. L., de Korte D., van den Berg T. K., Zeerleder S. S., Kuijpers T. W. Characterization of buffy coat-derived granulocytes for clinical use: a comparison with granulocyte colony-stimulating factor/dexamethasone-pretreated donor-derived products Vox sanguinis 2017;112 (2):173-182 [PubMed]
  • Stroo Ingrid, Zeerleder Sacha, Ding Chao, Luken Brenda M., Roelofs Joris J. T. H., de Boer Onno J., Meijers Joost C. M., Castellino Francis J., van 't Veer Cornelis, van der Poll Tom Coagulation factor XI improves host defence during murine pneumonia-derived sepsis independent of factor XII activation Thrombosis and haemostasis 2017;117 (8):1601-1614 [PubMed]
  • Marsman Gerben, von Richthofen Helen, Bulder Ingrid, Lupu Florea, Hazelzet Jan, Luken Brenda M., Zeerleder Sacha DNA and factor VII-activating protease protect against the cytotoxicity of histones Blood advances 2017;1 (26):2491-2502 [PubMed]
  • Sins J. W. R., Schimmel M., Luken B. M., Nur E., Zeerleder S. S., van Tuijn C. F. J., Brandjes D. P. M., Kopatz W. F., Urbanus R. T., Meijers J. C. M., Biemond B. J., Fijnvandraat K. Dynamics of von Willebrand factor reactivity in sickle cell disease during vaso-occlusive crisis and steady state Journal of thrombosis and haemostasis 2017;15 (7):1392-1402 [PubMed]
  • Marsman Gerben Factor VII-activating protease: Unraveling the release and regulation of dead cell nuclear damps: Unraveling the release and regulation of dead cell nuclear damps 2017. 213p. ISBN 9789461699954. [UvA Dissertations Online] University of Amsterdam. (Supervisors: Zeerleder S. S., van Ham S. M.; Co-supervisor: Luken B. M.)
  • Stroo Ingrid, Yang Jack, de Boer Johannes Daan, Roelofs Joris J. T. H., van 't Veer Cornelis, Castellino Francis J., Zeerleder Sacha, van der Poll Tom Factor XI deficiency enhances the pulmonary allergic response to house dust mite in mice independent of factor XII American journal of physiology. Lung cellular and molecular physiology 2017;312 (2):L163-L171 [PubMed]
  • Stroo Ingrid, Yang Jack, Anas Adam A., de Boer J. Daan, van Mierlo Gerard, Roem Dorina, Wouters Diana, Engel Ruchira, Roelofs Joris J. T. H., van 't Veer Cornelis, van der Poll Tom, Zeerleder Sacha Human plasma-derived C1 esterase inhibitor concentrate has limited effect on house dust mite-induced allergic lung inflammation in mice PLoS ONE 2017;12 (10):e0186652 [PubMed]
  • van der Valk Fleur M., Kuijk Carlijn, Verweij Simone L., Stiekema Lotte C. A., Kaiser Y., Zeerleder Sacha, Nahrendorf Matthias, Voermans Carlijn, Stroes Erik S. G. Increased haematopoietic activity in patients with atherosclerosis European heart journal 2017;38 (6):425-432 [PubMed]
  • Schimmel Marein, Luken Brenda M., Nur Erfan, van Tuijn Charlotte F. J., Sins Joep W., Brandjes Dees P. M., Zeerleder Sacha S., Biemond Bart J. Inflammatory and endothelial markers during vaso-occlusive crisis and acute chest syndrome in sickle cell disease American journal of hematology 2017;92 (11):E634-E636 [PubMed]
  • van der Meer Anne Jan, Zeerleder Sacha, Blok Dana C., Kager Liesbeth M., Lede Ivar O., Rahman Wahid, Afroz Rumana, Ghose Aniruddha, Visser Caroline E., Zahed Abu Shahed Md, Husain Md Anwar, Alam Khan Mashrequl, Barua Pravat Chandra, Hassan Mahtabuddin, Tayab Md Abu, Dondorp Arjen M., van der Poll Tom Neutrophil extracellular traps in patients with pulmonary tuberculosis Respiratory research 2017;18 (1):181 [PubMed]
  • Emmens Reindert W., Wouters Diana, Zeerleder Sacha, van Ham S. Marieke, Niessen Hans W. M., Krijnen Paul A. J. On the value of therapeutic interventions targeting the complement system in acute myocardial infarction Translational research 2017;182:103-122 [PubMed]
  • Sonneveld Myrthe E., de Haas Masja, Koeleman Carolien, de Haan Noortje, Zeerleder Sacha S., Ligthart Peter C., Wuhrer Manfred, van der Schoot C. Ellen, Vidarsson Gestur Patients with IgG1-anti-red blood cell autoantibodies show aberrant Fc-glycosylation Scientific reports 2017;7 (1):8187 [PubMed]
  • Smilde Bernard J., Woudstra Linde, Fong Hing Gene, Wouters Diana, Zeerleder Sacha, Murk Jean-Luc, van Ham Marieke, Heymans Stephane, Juffermans Lynda J. M., van Rossum Albert C., Niessen Hans W. M., Krijnen Paul A. J., Emmens Reindert W. Reply to the letter to the editor "Is colchicine really harmful in viral myocarditis?" International journal of cardiology 2017;229:43 [PubMed]
  • Schimmel Marein Sickle cell disease: Pathogenesis and biomarkers 2017. 205p. ISBN 9789492683441. [UvA Dissertations Online] University of Amsterdam. (Supervisors: Brandjes D. P. M. , van Oers M. H. J.; Co-supervisors: Biemond B. J. , Zeerleder S. S.)
  • Boshuizen M., van der Ploeg K., von Bonsdorff L., Biemond B. J., Zeerleder S. S., van Bruggen R., Juffermans N. P. Therapeutic use of transferrin to modulate anemia and conditions of iron toxicity Blood reviews 2017;31 (6):400-405 [PubMed]

2016

  • Hofman Zonne L. M., Relan Anurag, Zeerleder Sacha, Drouet Christian, Zuraw Bruce, Hack C. Erik Angioedema attacks in patients with hereditary angioedema: Local manifestations of a systemic activation process Journal of allergy and clinical immunology 2016;138 (2):359-366 [PubMed]
  • Balvers Kirsten, Saleh Sarah, Zeerleder Sacha S., Klinkspoor J. Henriette, Goslings J. Carel, Juffermans Nicole P. Are there any alternatives for transfusion of AB plasma as universal donor in an emergency release setting? Transfusion 2016;56 (6):1469-1474 [PubMed]
  • Heestermans Marco, Cunha Marisa L. R., Reitsma Pieter H., Zeerleder Sacha S., Middeldorp Saskia, van Vlijmen Bart J. M. Circulating nucleosomes and elastase α1-antitrypsin complexes and the novel thrombosis susceptibility locus SLC44A2 Thrombosis research 2016;142:8-10 [PubMed]
  • Smilde Bernard J., Woudstra Linde, Fong Hing Gene, Wouters Diana, Zeerleder Sacha, Murk Jean-Luc, van Ham Marieke, Heymans Stephane, Juffermans Lynda J. M., van Rossum Albert C., Niessen Hans W. M., Krijnen Paul A. J., Emmens Reindert W. Colchicine aggravates coxsackievirus B3 infection in mice International journal of cardiology 2016;216:58-65 [PubMed]
  • Pouw Richard B., Brouwer Mieke C., Geissler Judy, van Herpen Laurens V., Zeerleder Sacha S., Wuillemin Walter A., Wouters Diana, Kuijpers Taco W. Complement Factor H-Related Protein 3 Serum Levels Are Low Compared to Factor H and Mainly Determined by Gene Copy Number Variation in CFHR3 PLoS ONE 2016;11 (3):e0152164 [PubMed]
  • Liaw Patricia C., Ito Takashi, Iba Toshiaki, Thachil Jecko, Zeerleder Sacha DAMP and DIC: The role of extracellular DNA and DNA-binding proteins in the pathogenesis of DIC Blood reviews 2016;30 (4):257-261 [PubMed]
  • Emmens Reindert W., Baylan Umit, Juffermans Lynda J. M., Karia Rashmi V., Ylstra Bauke, Wouters Diana, Zeerleder Sacha, Simsek Suat, van Ham Marieke, Niessen Hans W. M., Krijnen Paul A. J. Endogenous C1-inhibitor production and expression in the heart after acute myocardial infarction Cardiovascular pathology 2016;25 (1):33-39 [PubMed]
  • Marsman Gerben, Zeerleder Sacha, Luken Brenda M. Extracellular histones, cell-free DNA, or nucleosomes: differences in immunostimulation Cell death & disease 2016;7 (12):e2518 [PubMed]
  • Marsman Gerben, Stephan Femke, de Leeuw Karina, Bulder Ingrid, Ruinard Jessica T., de Jong Jan, Westra Johanna, Bultink Irene E. M., Voskuyl Alexandre E., Aarden Lucien A., Luken Brenda M., Kallenberg Cees G. M., Zeerleder Sacha FSAP-mediated nucleosome release from late apoptotic cells is inhibited by autoantibodies present in SLE European journal of immunology 2016;46 (3):762-771 [PubMed]
  • Zeerleder Sacha, Levi Marcel Hereditary and acquired C1-inhibitor-dependent angioedema: from pathophysiology to treatment Annals of medicine 2016;48 (4):256-267 [PubMed]
  • Begieneman Mark Pv, Emmens Reindert W., Rijvers Liza, Woudstra Linde, Paulus Walter J., Kubat Bela, Vonk Alexander Ba, van Rossum Albert C., Wouters Diana, Zeerleder Sacha, van Ham Marieke, Schalkwijk Casper G., Niessen Hans Wm, Krijnen Paul Aj Myocardial infarction induces atrial inflammation that can be prevented by C1-esterase inhibitor Journal of clinical pathology 2016;69 (12):1093-1099 [PubMed]
  • de Beer Friso, Lagrand Wim, Glas Gerie J., Beurskens Charlotte J. P., van Mierlo Gerard, Wouters Diana, Zeerleder Sacha, Roelofs Joris J. T. H., Juffermans Nicole P., Horn Janneke, Schultz Marcus J. Nebulized C1-Esterase Inhibitor does not Reduce Pulmonary Complement Activation in Rats with Severe Streptococcus Pneumoniae Pneumonia Cell biochemistry and biophysics 2016;74 (4):545-552 [PubMed]
  • Heestermans Marco, Salloum-Asfar Salam, Salvatori Daniela, Laghmani El Houari, Luken Brenda M., Zeerleder Sacha S., Spronk Henri M. H., Korporaal Suzanne J., Wagenaar Gerry T. M., Reitsma Pieter H., van Vlijmen Bart J. M. Role of platelets, neutrophils, and factor XII in spontaneous venous thrombosis in mice Blood 2016;127 (21):2630-2637 [PubMed]
  • Bots M., de Grouw E. P. L. M., van Rooyen-Schreurs I. H. M., van den Akker G. J., Sturk A., Klinkspoor J. H., Zeerleder S. S. Strategies to reduce wastage of red blood cell units Vox sanguinis 2016;110 (2):143-149 [PubMed]
  • Engert Andreas, Balduini Carlo, Brand Anneke, Coiffier Bertrand, Cordonnier Catherine, Döhner Hartmut, de Wit Thom Duyvené, Eichinger Sabine, Fibbe Willem, Green Tony, de Haas Fleur, Iolascon Achille, Jaffredo Thierry, Rodeghiero Francesco, Salles Gilles, Schuringa Jan Jacob, André Marc, Andre-Schmutz Isabelle, Bacigalupo Andrea, Bochud Pierre-Yves, Boer Monique den, Bonini Chiara, Camaschella Clara, Cant Andrew, Cappellini Maria Domenica, Cazzola Mario, Celso Cristina Lo, Dimopoulos Meletios, Douay Luc, Dzierzak Elaine, Einsele Hermann, Ferreri Andrés, de Franceschi Lucia, Gaulard Philippe, Gottgens Berthold, Greinacher Andreas, Gresele Paolo, Gribben John, de Haan Gerald, Hansen John-Bjarne, Hochhaus Andreas, Kadir Rezan, Kaveri Srini, Kouskoff Valerie, Kühne Thomas, Kyrle Paul, Ljungman Per, Reitsma Pieter, Zaaijer Hans, Zeerleder Sacha The European Hematology Association Roadmap for European Hematology Research: a consensus document Haematologica 2016;101 (2):115-208 [PubMed]
  • van der Meer Anne Jan, Achouiti Achmed, van der Ende Arie, Soussan Aicha A., Florquin Sandrine, de Vos Alex, Zeerleder Sacha S., van der Poll Tom Toll-like receptor 9 enhances bacterial clearance and limits lung consolidation in murine pneumonia caused by methicillin resistant Staphylococcus aureus Molecular medicine (Cambridge, Mass.) 2016;22:292-299 [PubMed]
  • Peters Anna L., van Hezel Maike E., Cortjens Bart, Tuip-de Boer Anita M., van Bruggen Robin, de Korte Dirk, Jonkers René E., Bonta Peter I., Zeerleder Sacha S., Lutter Rene, Juffermans Nicole P., Vlaar Alexander P. J. Transfusion of 35-Day Stored RBCs in the Presence of Endotoxemia Does Not Result in Lung Injury in Humans Critical care medicine 2016;44 (6):e412-e419 [PubMed]
  • Choi G., Snijder S., Zeerleder Sacha S. Uw diagnose? Nederlands tijdschrift voor hematologie 2016;2016 (13):62-63
  • Begieneman Mark P. V., Emmens Reindert W., Rijvers Liza, Kubat Bela, Paulus Walter J., Vonk Alexander B. A., Rozendaal Lawrence, Biesbroek P. Stefan, Wouters Diana, Zeerleder Sacha, van Ham Marieke, Heymans Stephane, van Rossum Albert C., Niessen Hans W. M., Krijnen Paul A. J. Ventricular myocarditis coincides with atrial myocarditis in patients Cardiovascular pathology 2016;25 (2):141-148 [PubMed]

2015

  • de Boer J. Daan, Berger Marieke, Majoor Christof J., Kager Liesbeth M., Meijers Joost C. M., Terpstra Sanne, Nieuwland Rienk, Boing Anita N., Lutter René, Wouters Diana, van Mierlo Gerard J., Zeerleder Sacha S., Bel Elisabeth H., van't Veer Cornelis, de Vos Alex F., van der Zee Jaring S., van der Poll Tom Activated protein C inhibits neutrophil migration in allergic asthma: a randomised trial European respiratory journal 2015;46 (6):1636-1644 [PubMed]
  • Solati Shabnam, Aarden Lucien, Zeerleder Sacha, Wouters Diana An improved monocyte activation test using cryopreserved pooled human mononuclear cells Innate immunity 2015;21 (7):677-684 [PubMed]
  • te Boome L. C. J., Mansilla C., van der Wagen L. E., Lindemans C. A., Petersen E. J., Spierings E., Thus K. A., Westinga K., Plantinga M., Bierings M., Broers A. E. C., Cuijpers M. L. H., van Imhoff G. W., Janssen J. J., Huisman C., Zeerleder S., Huls G., Boelens J. J., Wulffraat N. M., Slaper-Cortenbach I. C. M., Kuball J. Biomarker profiling of steroid-resistant acute GVHD in patients after infusion of mesenchymal stromal cells Leukemia 2015;29 (9):1839-1846 [PubMed]
  • Meulenbroek Elisabeth M., de Haas Masja, Brouwer Conny, Folman Claudia, Zeerleder Sacha S., Wouters Diana Complement deposition in autoimmune hemolytic anemia is a footprint for difficult-to-detect IgM autoantibodies Haematologica 2015;100 (11):1407-1414 [PubMed]
  • Wouters Diana, Zeerleder Sacha Complement inhibitors to treat IgM-mediated autoimmune hemolysis Haematologica 2015;100 (11):1388-1395 [PubMed]
  • de Jong H. K., Koh G. C. K. W., Bulder I., Stephan F., Wiersinga W. J., Zeerleder S. S. Diabetes-independent increase of factor VII-activating protease activation in patients with Gram-negative sepsis (melioidosis) Journal of thrombosis and haemostasis 2015;13 (1):41-46 [PubMed]
  • Balvers Kirsten, Coppens Michiel, van Dieren Susan, van Rooyen-Schreurs Ingeborg H. M., Klinkspoor Henriëtte J., Zeerleder Sacha S., Baumann Holger M., Goslings J. Carel, Juffermans Nicole P. Effects of a hospital-wide introduction of a massive transfusion protocol on blood product ratio and blood product waste Journal of emergencies, trauma, and shock 2015;8 (4):199-204 [PubMed]
  • Engel Ruchira, Rensink Irma, Roem Dorina, Brouwer Mieke, Kalei Asma, Perry Dawn, Zeerleder Sacha, Wouters Diana, Hamann Dörte ELISA to measure neutralizing capacity of anti-C1-inhibitor antibodies in plasma of angioedema patients Journal of immunological methods 2015;426:114-119 [PubMed]
  • Huson Michaëla A. M., Wouters Diana, van Mierlo Gerard, Grobusch Martin P., Zeerleder Sacha S., van der Poll Tom HIV Coinfection Enhances Complement Activation During Sepsis Journal of infectious diseases 2015;212 (3):474-483 [PubMed]
  • Huson Michaëla A. M., Zeerleder Sacha S., van Mierlo Gerard, Wouters Diana, Grobusch Martin P., van der Poll Tom HIV infection is associated with elevated nucleosomes in asymptomatic patients and during sepsis or malaria Journal of infection 2015;71 (2):266-269 [PubMed]
  • Paris Daniel H., Stephan Femke, Bulder Ingrid, Wouters Diana, van der Poll Tom, Newton Paul N., Day Nicholas P. J., Zeerleder Sacha Increased Nucleosomes and Neutrophil Activation Link to Disease Progression in Patients with Scrub Typhus but Not Murine Typhus in Laos PLoS neglected tropical diseases 2015;9 (8):e0003990 [PubMed]
  • Asgarian-Omran Hossein, Amirzargar Ali Akbar, Zeerleder Sacha, Mahdavi Marzieh, van Mierlo Gerard, Solati Shabnam, Jeddi-Tehrani Mahmood, Rabbani Hodjatallah, Aarden Leucien, Shokri Fazel Interaction of Bordetella pertussis filamentous hemagglutinin with human TLR2: identification of the TLR2-binding domain APMIS 2015;123 (2):156-162 [PubMed]
  • Meulenbroek Elisabeth M., Wouters Diana, Zeerleder Sacha S. Lyse or not to lyse: Clinical significance of red blood cell autoantibodies Blood reviews 2015;29 (6):369-376 [PubMed]
  • Achouiti Ahmed, Vogl Thomas, van der Meer Anne J., Stroo Ingrid, Florquin Sandrine, de Boer Onno J., Roth Johannes, Zeerleder Sacha, van 't Veer Cornelis, de Vos Alex F., van der Poll Tom Myeloid-related protein-14 deficiency promotes inflammation in staphylococcal pneumonia European respiratory journal 2015;46 (2):464-473 [PubMed]
  • van Bijnen S. T. A., Wouters D., van Mierlo G. J., Muus P., Zeerleder S. Neutrophil activation and nucleosomes as markers of systemic inflammation in paroxysmal nocturnal hemoglobinuria: effects of eculizumab Journal of thrombosis and haemostasis 2015;13 (11):2004-2011 [PubMed]
  • Keizer Mischa P., Pouw Richard B., Kamp Angela M., Patiwael Sanne, Marsman Gerben, Hart Margreet H., Zeerleder Sacha, Kuijpers Taco W., Wouters Diana TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2 European journal of immunology 2015;45 (2):544-550 [PubMed]

2014

  • de Witte M. A., Determann R. M., Zeerleder S. S. A man with "black fingers". Cold agglutinin disease (CAD) Netherlands journal of medicine 2014;72 (1):35, 39-35, 40 [PubMed]
  • Stephan F., Bulder I., Luken B. M., Hazelzet J., Wuillemin W. A., Zeerleder S. Complexes of factor VII-activating protease with plasminogen activator inhibitor-1 in human sepsis Thrombosis and haemostasis 2014;112 (1):219-221 [PubMed]
  • Stephan Femke, Marsman Gerben, Bakker Liza M., Bulder Ingrid, Stavenuiter Fabian, Aarden Lucien A., Zeerleder Sacha Cooperation of Factor VII-Activating Protease and Serum DNase I in the Release of Nucleosomes From Necrotic Cells Arthritis & rheumatology (Hoboken, N.J.) 2014;66 (3):686-693 [PubMed]
  • Emmens Reindert W., Naaijkens Benno A., Roem Dorina, Kramer Klaas, Wouters Diana, Zeerleder Sacha, van Ham Marieke S., Niessen Hans W., Krijnen Paul A. Evaluating the efficacy of subcutaneous C1-esterase inhibitor administration for use in rat models of inflammatory diseases Drug delivery 2014;21 (4):302-306 [PubMed]
  • Stephan Femke Factor VII-activating protease: Mechanism and regulation of nucleosome release from dead cells 2014. 131p. ISBN 9789462591776. [UvA Dissertations Online] University of Amsterdam. (Supervisor: Aarden L. A.; Co-supervisor: Zeerleder S. S.)
  • Emmens Reindert W., Woudstra Linde, Papageorgiou Anna, Carai Paolo, Smit Stefanie, Seven-Deniz Sevgi, Rozendaal Lawrence, Paulus Walter J., Wouters Diana, Zeerleder Sacha, Murk Jean-Luc, van Ham Marieke S., Heymans Stephane, van Rossum Albert C., Niessen Hans W. M., Krijnen Paul A. J. Lymphocytes infiltrate the quadriceps muscle in lymphocytic myocarditis patients: a potential new diagnostic tool Canadian journal of cardiology 2014;30 (12):1547-1554 [PubMed]
  • Meulenbroek Elisabeth M., Wouters Diana, Zeerleder Sacha Methods for quantitative detection of antibody-induced complement activation on red blood cells Journal of visualized experiments 2014;2014 (83):e51161 [PubMed]
  • de Jong Hanna K., Koh Gavin C. K. W., Achouiti Ahmed, van der Meer Anne J., Bulder Ingrid, Stephan Femke, Roelofs Joris J. T. H., Day Nick P. J., Peacock Sharon J., Zeerleder Sacha, Wiersinga W. Joost Neutrophil extracellular traps in the host defense against sepsis induced by Burkholderia pseudomallei (melioidosis) Intensive Care Medicine Experimental 2014;2 (1):21 [PubMed]
  • de Rooij Sophia E., Zeerleder Sacha, Burtman David, van Munster Barbara C. Nucleosomes in individuals with hip fracture with and without delirium Journal of the American Geriatrics Society 2014;62 (2):395-396 [PubMed]
  • de Beer F. M., Aslami H., Hoeksma J., van Mierlo G., Wouters D., Zeerleder S., Roelofs J. J. T. H., Juffermans N. P., Schultz M. J., Lagrand W. K. Plasma-derived human C1-esterase inhibitor does not prevent mechanical ventilation-induced pulmonary complement activation in a rat model of Streptococcus pneumoniae pneumonia Cell biochemistry and biophysics 2014;70 (2):795-803 [PubMed]
  • Müller M. C. A., Stroo I., Wouters D., Zeerleder S. S., Roelofs J. J. T. H., Boon L., Vroom M. B., Juffermans N. P. The effect of C1-inhibitor in a murine model of transfusion-related acute lung injury Vox sanguinis 2014;107 (1):71-75 [PubMed]

2013

  • Wouters Diana, Stephan Femke, Strengers Paul, de Haas Masja, Brouwer Conny, Hagenbeek Anton, van Oers Marinus H. J., Zeerleder Sacha C1-esterase inhibitor concentrate rescues erythrocytes from complement-mediated destruction in autoimmune hemolytic anemia Blood 2013;121 (7):1242-1244 [PubMed]
  • van Montfoort Maurits L., Stephan Femke, Lauw Mandy N., Hutten Barbara A., van Mierlo Gerard J., Solati Shabnam, Middeldorp Saskia, Meijers Joost C. M., Zeerleder Sacha Circulating nucleosomes and neutrophil activation as risk factors for deep vein thrombosis Arteriosclerosis, thrombosis, and vascular biology 2013;33 (1):147-151 [PubMed]
  • Kager L. M., Wiersinga W. J., Roelofs J. J., Meijers J. C. M., Zeerleder S. S., Esmon C. T., van't Veer C., van der Poll T. Endogenous protein C has a protective role during Gram-negative pneumosepsis (melioidosis) Journal of thrombosis and haemostasis 2013;11 (2):282-292 [PubMed]
  • Kager Liesbeth M., de Boer J. Daan, Bresser Paul, van der Zee Jaring S., Zeerleder Sacha, Meijers Joost C. M., van 't Veer Cornelis, van der Poll Tom Intrabronchial activated protein C enhances lipopolysaccharide-induced pulmonary responses European respiratory journal 2013;42 (1):188-197 [PubMed]
  • Schimmel Marein, Nur Erfan, Biemond Bart J., van Mierlo Gerard J., Solati Shabnam, Brandjes Dees P., Otten Hans-Martin, Schnog John-John, Zeerleder Sacha Nucleosomes and neutrophil activation in sickle cell disease painful crisis Haematologica 2013;98 (11):1797-1803 [PubMed]

2012

  • Zeerleder Sacha, van Oers Marinus Aleukemic variant of mast cell leukemia Blood 2012;119 (9):1961 [PubMed]
  • Krijnen Paul A. J., Kupreishvili Koba, de Vries Margreet R., Schepers Abbey, Stooker Wim, Vonk Alexander B. A., Eijsman Leon, van Hinsbergh Victor W. M., Zeerleder Sacha, Wouters Diana, van Ham Marieke, Quax Paul H. A., Niessen Hans W. M. C1-esterase inhibitor protects against early vein graft remodeling under arterial blood pressure Atherosclerosis 2012;220 (1):86-92 [PubMed]
  • Zeerleder Sacha, Stephan Femke, Emonts Marieke, de Kleijn Ester D., Esmon Charles T., Varadi Katalin, Hack Cornelis Erik, Hazelzet Jan A. Circulating nucleosomes and severity of illness in children suffering from meningococcal sepsis treated with protein C Critical care medicine 2012;40 (12):3224-3229 [PubMed]
  • Cicardi M., Bork K., Caballero T., Craig T., Li H. H., Longhurst H., Reshef A., Zuraw B., Werner Aberer, Aygören-Pürsün Emel, Banerji Aleena, Bjorkander Janne, Boccon-Gibod Isabelle, Konrad Bork, Bouillet Laurence, Bova Maria, Bowen Tom, Branco Ferreira Manuel, Bygum Anette, Caballero Teresa, Cancian Mauro, Castel-Branco Maria Graça, Cicardi Marco, Craig Timothy, de Carolis Caterina, Mihály Enikö, Josè Fabiani, Farkas Henriette, Gompels Mark, Gower Richard, Groffik Adriane, Grumach Anete, Guillarte Mar, Hernandez Landeros Maria Esthela, Kaplan Allen, Leibovich Iris, Li Henry, Lock Bob, Longhurst Hilary, Lumry William, Malbran Alejandro, Martinez-Saguer Immaculada, Campos Matta, Maurer Marcus, Moldovan Dumitru, Montinaro Vincenzo, Nieto Sandra, Nordenfelt Patrik, Obtulovicz Krystana, Zeerleder Sacha Evidence-based recommendations for the therapeutic management of angioedema owing to hereditary C1 inhibitor deficiency: consensus report of an International Working Group Allergy 2012;67 (2):147-157 [PubMed]
  • Kanse Sandip M., Gallenmueller Andrea, Zeerleder Sacha, Stephan Femke, Rannou Olivier, Denk Stephanie, Etscheid Michael, Lochnit Guenter, Krueger Marcus, Huber-Lang Markus Factor VII-Activating Protease Is Activated in Multiple Trauma Patients and Generates Anaphylatoxin C5a Journal of immunology (Baltimore, Md. 2012;188 (6):2858-2865 [PubMed]
  • Stephan F., Aarden L. A., Zeerleder S. FSAP, a new player in inflammation? Hamostaseologie 2012;32 (1):51-55 [PubMed]
  • Begieneman Mark P. V., Kubat Bela, Ulrich Magda M. W., Hahn Nynke E., Stumpf-Stolker Yvette, Tempelaars Miranda, Middelkoop Esther, Zeerleder Sacha, Wouters Diana, van Ham Marieke S., Niessen Hans W. M., Krijnen Paul A. J. Prolonged C1 Inhibitor Administration Improves Local Healing of Burn Wounds and Reduces Myocardial Inflammation in a Rat Burn Wound Model Journal of burn care & research 2012;33 (4):544-551 [PubMed]
  • Stephan F., Dienava-Verdoold I., Bulder I., Wouters D., Mast A. E., te Velthuis H., Aarden L. A., Zeerleder S. Tissue factor pathway inhibitor is an inhibitor of factor VII-activating protease Journal of thrombosis and haemostasis 2012;10 (6):1165-1171 [PubMed]
  • Vlaar Alexander P. J., Hofstra Jorrit J., Determann Rogier M., Veelo Denise P., Paulus Frederique, Levi Marcel, Zeerleder Sacha, Vroom Margreeth B., Schultz Marcus J., Juffermans Nicole P. Transfusion-related acute lung injury in cardiac surgery patients is characterized by pulmonary inflammation and coagulopathy: A prospective nested case-control study Critical care medicine 2012;40 (10):2813-2820 [PubMed]

2011

  • Stephan Femke, Hazelzet Jan A., Bulder Ingrid, Boermeester Marja A., van Till Jw Olivier, van der Poll Tom, Wuillemin Walter A., Aarden Lucien A., Zeerleder Sacha Activation of factor VII-activating protease in human inflammation: a sensor for cell death Critical care (London, England) 2011;15 (2):R110 [PubMed]
  • Zeerleder S. Autoimmune haemolytic anaemia - a practical guide to cope with a diagnostic and therapeutic challenge Netherlands journal of medicine 2011;69 (4):177-184 [PubMed]
  • Zeerleder Sacha C1-inhibitor: more than a serine protease inhibitor Seminars in thrombosis and hemostasis 2011;37 (4):362-374 [PubMed]
  • van der Windt Gerritje J. W., Schouten Marcel, Zeerleder Sacha, Florquin Sandrine, van der Poll Tom CD44 Is Protective during Hyperoxia-Induced Lung Injury American journal of respiratory cell and molecular biology 2011;44 (3):377-383 [PubMed]
  • Vlaar A. P. J., Rietdijk S. T., Zeerleder S. S., Boerman T., Beuers U. Malignancies associated with chronic hepatitis C: case report and review of the literature Netherlands journal of medicine 2011;69 (5):211-215 [PubMed]

2010

  • ter Weeme M., Vonk A. B. A., Kupreishvili K., van Ham M., Zeerleder S., Wouters D., Stooker W., Eijsman L., van Hinsbergh V. W. M., Krijnen P. A. J., Niessen H. W. M. Activated complement is more extensively present in diseased aortic valves than naturally occurring complement inhibitors: a sign of ongoing inflammation European journal of clinical investigation 2010;40 (1):4-10 [PubMed]

2008

  • Wouters Diana, Wagenaar-Bos Ineke, van Ham Marieke, Zeerleder Sacha C1 inhibitor: just a serine protease inhibitor? New and old considerations on therapeutic applications of C1 inhibitor Expert opinion on biological therapy 2008;8 (8):1225-1240 [PubMed]
  • Zeerleder Sacha, Zwart Bas, te Velthuis Henk, Stephan Femke, Manoe Rishi, Rensink Irma, Aarden Lucien A. Nucleosome-releasing factor: a new role for factor VII-activating protease (FSAP) FASEB journal 2008;22 (12):4077-4084 [PubMed]

2007

  • Kremer Hovinga J. A., Zeerleder S., Kessler P., Romani de Wit T., van Mourik J. A., Hack C. E., ten Cate H., Reitsma P. H., Wuillemin W. A., Lämmle B. ADAMTS-13, von Willebrand factor and related parameters in severe sepsis and septic shock Journal of thrombosis and haemostasis 2007;5 (11):2284-2290 [PubMed]
  • Zeerleder Sacha, Zwart Bas, te Veldhuis Henk, Manoe Rishi, Bulder Ingrid, Rensink Irma, Aarden Lucien A. A plasma nucleosome releasing factor (NRF) with serine protease activity is instrumental in removal of nucleosomes from secondary necrotic cells FEBS letters 2007;581 (28):5382-5388 [PubMed]
  • Zeerleder Sacha, Schroeder Verena, Lämmle Bernhard, Wuillemin Walter A., Hack C. Erik, Kohler Hans Peter Factor XIII in severe sepsis and septic shock Thrombosis research 2007;119 (3):311-318 [PubMed]
  • Zeerleder S. S. Studies on inflammatory and coagulation pathways in humans with sepsis 2007. 142p. ISBN 9789090214078. [UvA Dissertations Online] University of Amsterdam. (Supervisors: Hack C. Erik, Wuillemin Walter A.)

2006

  • Voves Claudia, Wuillemin Walter A., Zeerleder Sacha International Society on Thrombosis and Haemostasis score for overt disseminated intravascular coagulation predicts organ dysfunction and fatality in sepsis patients Blood coagulation & fibrinolysis 2006;17 (6):445-451 [PubMed]
  • Zeerleder Sacha, Schroeder Verena, Hack C. Erik, Kohler Hans Peter, Wuillemin Walter A. TAFI and PAI-1 levels in human sepsis Thrombosis research 2006;118 (2):205-212 [PubMed]
  • Bouman Catherine S. C., de Pont Anne-Cornélie J. M., Meijers Joost C. M., Bakhtiari Kamran, Roem Dorina, Zeerleder Sacha, Wolbink Gertjan, Korevaar Johanna C., Levi Marcel, de Jonge Evert The effects of continuous venovenous hemofiltration on coagulation activation Critical care (London, England) 2006;10 (5):R150 [PubMed]
  • Zeerleder Sacha The struggle to detect circulating DNA Critical care (London, England) 2006;10 (3):142 [PubMed]

2005

  • Zeerleder Sacha, Hack C. Erik, Caliezi Christoph, van Mierlo Gerard, Eerenberg-Belmer Anke, Wolbink Angela, Wuillenmin Walter A. Activated cytotoxic T cells and NK cells in severe sepsis and septic shock and their role in multiple organ dysfunction Clinical immunology (Orlando, Fla.) 2005;116 (2):158-165 [PubMed]
  • Zeerleder Sacha, Voves Claudia, Wuillemin Walter A. Comment on hemostatic markers and the sepsis-related organ failure assessment score in patients with disseminated intravascular coagulation in an intensive care unit by Okabayashi et al American journal of hematology 2005;79 (4):344 [PubMed]
  • Zeerleder Sacha, Hack C. Erik, Wuillemin Walter A. Disseminated intravascular coagulation in sepsis Chest 2005;128 (4):2864-2875 [PubMed]

2003

  • Zeerleder Sacha, Caliezi Christoph, van Mierlo Gerard, Eerenberg-Belmer Anke, Sulzer Irmela, Hack C. Erik, Wuillemin Walter A. Administration of C1 inhibitor reduces neutrophil activation in patients with sepsis Clinical and diagnostic laboratory immunology 2003;10 (4):529-535 [PubMed]
  • Zeerleder S., Zürcher Zenklusen R., Hack C. E., Wuillemin W. A. Disseminated intravascular coagulation in meningococcal sepsis. Case 7 Hamostaseologie 2003;23 (3):125-130 [PubMed]
  • Zeerleder Sacha, Zwart Bas, Wuillemin Walter A., Aarden Lucien A., Groeneveld A. B. Johan, Caliezi Christoph, van Nieuwenhuijze Annemarie E. M., van Mierlo Gerard J., Eerenberg Anke J. M., Lämmle Bernhard, Hack C. Erik Elevated nucleosome levels in systemic inflammation and sepsis Critical care medicine 2003;31 (7):1947-1951 [PubMed]

2002

  • Caliezi Christoph, Zeerleder Sacha, Redondo Maurice, Regli Bruno, Rothen Hans-Ulrich, Zürcher-Zenklusen Regula, Rieben Robert, Devay Jan, Hack C. Erik, Lämmle Bernhard, Wuillemin Walter A. C1-inhibitor in patients with severe sepsis and septic shock: beneficial effect on renal dysfunction Critical care medicine 2002;30 (8):1722-1728 [PubMed]
  • Zeerleder Sacha, Mauron Thomas, Lämmle Bernhard, Wuillemin Walter A. Effect of low-molecular weight dextran sulfate on coagulation and platelet function tests Thrombosis research 2002;105 (5):441-446 [PubMed]
  • Wuillemin Walter A., Gasser K. atherina M., Zeerleder Sacha S., Lämmle Bernhard Evaluation of a Platelet Function Analyser (PFA-100) in patients with a bleeding tendency Swiss medical weekly 2002;132 (31-32):443-448 [PubMed]

2001

  • Zeerleder S., Hack C. E., Caliezi C., Hebeisen R., Wuillemin W. A. Bacillus Calmette-Guérin sepsis: shift of an intended local toward a detrimental systemic cytotoxic immune response Blood 2001;98 (3):890-891 [PubMed]
  • Hack C. E., Zeerleder S. The endothelium in sepsis: source of and a target for inflammation Critical care medicine 2001;29 (7 Suppl):S21-S27 [PubMed]

2000

  • Caliezi C., Wuillemin W. A., Zeerleder S., Redondo M., Eisele B., Hack C. E. C1-Esterase inhibitor: an anti-inflammatory agent and its potential use in the treatment of diseases other than hereditary angioedema Pharmacological reviews 2000;52 (1):91-112 [PubMed]

1999

  • Zeerleder S., Caliezi C., Redondo M., Devay J., Wuillemin W. A. Aktivierung der plasmatischen Kaskadensysteme bei Sepsis: Rolle von C1-Inhibitor Schweizerische medizinische Wochenschrift 1999;129 (39):1410-1417 [PubMed]
  • Zeerleder S., Asmis L., Redondo M., Sulzer I., Lämmle B. Eine Patientin mit isolierter Verlängerung der aPTT ohne Anamnese einer hämorrhagischen Diathese: schwerer hereditärer Faktor XII-Mangel Therapeutische Umschau. Revue therapeutique 1999;56 (9):509-512 [PubMed]
  • Fontana S., Zeerleder S., Lämmle B. Entgleiste orale Antikoagulation mit sehr hohen INR-Werten und schlechtem Ansprechen auf perorales Vitamin K--Cholestase als mögliche Ursache Therapeutische Umschau. Revue therapeutique 1999;56 (9):477-480 [PubMed]
  • Redondo M., Solenthaler M., Zeerleder S., Wuillemin W. A. Isoliert verlängerte aPTT mit anamnestischer hämorrhagischer Diathese--Schwerer FXI-Mangel Therapeutische Umschau. Revue therapeutique 1999;56 (9):502-504 [PubMed]
  • Zeerleder S., Schloesser M., Redondo M., Wuillemin W. A., Engel W., Furlan M., Lämmle B. Reevaluation of the incidence of thromboembolic complications in congenital factor XII deficiency--a study on 73 subjects from 14 Swiss families Thrombosis and haemostasis 1999;82 (4):1240-1246 [PubMed]

1997

  • Hassink R. I., Zeerleder S. S., Truttmann A. C., Bianchetti M. G. Hemolytic-uremic syndrome after Escherichia coli urinary tract infection Pediatric infectious disease journal 1997;16 (8):828 [PubMed]
  • Schloesser M., Zeerleder S., Lutze G., Halbmayer W. M., Hofferbert S., Hinney B., Koestering H., Lämmle B., Pindur G., Thies K., Köhler M., Engel W. Mutations in the human factor XII gene Blood 1997;90 (10):3967-3977 [PubMed]
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