Laboratory for Medical Immunology (LMIM)

The Laboratory for Medical Immunology (LMIM) performs laboratory tests for the diagnosis and follow-up of immunological disorders.

Who are we?

The laboratory is equipped with a broad spectrum state-of-the-art techniques and instrumentation. Two specifically trained Medical Immunologists supervise the laboratory and are in close contact with clinicians for interpretation of test results and advice. A group of well trained technicians stands for a high standard of performance. LMIM also participates in clinical research projects. LMIM is accreditated by the Dutch Accreditation Council (RvA) according to the rules given in NEN-EN-ISO 15189:2012 (registration number M153). LMIM is part of the Department of Experimental Immunology where both fundamental and translational immunological research is performed. This enables an up-to-date insight into recent advances in immunological topics and implementation of new diagnostic tests. Since April 2011 LMIM is participating in a Lean program which leads to continuous improvement of the quality of our service.

The laboratory test relate to:

  • Primary and secondary immunodeficiency
  • Autoimmune diseases: e.g. Rheumatoid Arthritis, SLE, Sjögren Syndrome, Systemic Sclerosis, Vasculitis, Myositis
  • Plasmacell dyscrasias: e.g. Multiple Myeloma, AL Amyloidosis
  • Inflammatory Bowel Disease: e.g. Ulcerative Colitis, Crohn's Disease
  • (Latent) Tuberculosis infection
  • Monitoring of immune therapies: e.g. B-cell depletion by Rituximab

What do we do?

Serology tests:

  • anti-nuclear antibodies (ANA)
  • anti-dsDNA-Nucleosome antibodies
  • anti-ENA
  • anti-tTG (tissue transglutaminase) IgA and IgG*
  • anti-deamidated gliadin IgG*
  • anti-smooth muscle
  • anti-mitochondria
  • anti-LKM-1
  • anti-parietal cells
  • antibodies specific for systemic sclerosis (immunoblot)
  • antibodies specific for myositis (immunoblot)
  • antibodies specific for auto immune liver diseases (immunoblot)
  • anti-phospholipase A2 receptor (anti-PLA2R)
  • anti-Aquaporin 4
  • ANCA (vasculitis)
  • anti-PR3
  • anti-MPO
  • anti-GBM
  • pANCA (colitis ulcerosa, autoimmune hepatitis, primary sclerosing cholangitis)
  • anti-CCP
  • Rheumatoid Factor (IgM)
  • IgD
  • antibodies against tetanus toxoid (before and after vaccination)
  • antibodies against Streptococcus pneumoniae (before and after vaccination)
  • function of classical and alternative pathway of the complement system
  • determination of M-proteins (serum and urine) by immunofixation
  • quantification of free light chains (serum)*
  • quantification and classsification of cryoglobulins

* authorisation and responsibility LMIM, practical realisation by the Department of Clinical Chemistry

Cellular tests:

  • T, B and NK cells (% and absolute number)
  • naïve, memory and effector T-cell subpopulations
  • naïve and memory B-cell subpopulations
  • enumeration of B-cells i.e. after Rituximab treatment (highly sensitive method)
  • CD4+ regulatory T-cells
  • activated T-cells (CD4+ and CD8+ T-cells)
  • TCRγδ T-cells
  • CD4-CD8-TCRαβ+ T-cells
  • iNKT-cells
  • Perforin and Granzyme-B
  • proliferative capacity of T, B and/or NK cells
  • IgG and IgM production upon B-cell stimulation in vitro**
  • NK-cell function: degranulation (CD107a) and cytotoxicity
  • NKG2D
  • CD40**
  • CD40L**


  • Calprotectin (in faeces)
  • IGRA (IFNγ release assay upon TB antigen stimulation)
  • Cryopreservation of PBMC, plasma and serum

** these assays are not validated and fall outside the scope of the ISO 15189 accreditation

We also perform diagnostic tests for external clients. For further details see the NVKC Wie-doet-wat-database or contact Dr. E.M.M. (Ester) van Leeuwen or Dr. P.A. (Paul) Baars.

Contact information

Head: Dr. E.M.M. van Leeuwen

Medical Immunologists: Dr. E.M.M. van Leeuwen / Dr. P.A. Baars

Address: Academic Medical Center, Department Experimental Immunology G1-111, Meibergdreef 9, 1105 AZ Amsterdam Head: +31-20-5667022

For questions regarding tests and/or results:  E-mail Medical Immunologists: / Telephone: +31-6-50063950

For questions regarding logistics: E-mail laboratory:

For complaints, remarks and/or suggestions for improvement: E-mail:


Tijdens de feestdagen zijn onze poliklinieken gesloten. Lees meer

Address for sending patient material for diagnostic tests

AMC Receptie LAKC,
Meibergdreef 9,
1105 AZ Amsterdam
The Netherlands

Naar de Nederlandstalige versie LMIM