Description / Indication
For analysis of accumulation of very long chain fatty acids in peroxisomal disorders (e.g. X-linked adrenoleucodystrofy (X-ALD)) we analyse C26:0-Lyso-PC (blood) and/or C26:0-Lyso-PC (fibroblasts).
Method
GC-MS
TAT
4 weeks (excl. culture time for fibroblasts).
Price
€ 216
Materials:
Plasma
- Amount: 500 µl
- Conditions: Dry ice.
- Specifics: EDTA plasma (preferred material), heparin plasma also suitable.
Cultured skin fibroblasts
- Conditions: Room temperature
- Specifics: Grow the fibroblasts to near confluence in a T-25 flask, fill the flask completely with sterile buffered (25mM Hepes) culture media supplemented with 10% serum and 1% penicilline-streptomycine. Cap the flasks tightly and wrap the cap with parafilm. Label the flask clearly with identifying information and date of collection, wrap the culture flaks in protective material and place it in a sealed plastic bag in the Styrofoam shipping box and ship AT ROOM TEMPERATURE by courier service. Fibroblasts need to arrive in our laboratory within 48 hours. Please complete the test request form and send it along with the sample.
If it is not possible to culture fibroblasts from a skin biopsy in a local institute, you can send a skin biopsy in sterile buffered (25mM Hepes) culture medium without serum and antibiotics AT ROOM TEMPERATURE by courier service. The skin biopsy needs to arrive in our laboratory within 48 hours. Please complete the test request form and send it along with the sample.